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The map label for this gene is ruvA

Identifier: 158336024

GI number: 158336024

Start: 2922482

End: 2923108

Strand: Reverse

Name: ruvA

Synonym: AM1_2883

Alternate gene names: 158336024

Gene position: 2923108-2922482 (Counterclockwise)

Preceding gene: 158336025

Following gene: 158336023

Centisome position: 44.95

GC content: 48.01

Gene sequence:

>627_bases
ATGATTGGTTTTCTGCAAGGGTATGTCGTTAGTCTTCAGCAAACCAGTACCCATCGAACCTTGTTGACTTTGGATGTGAA
CCAGGTCGGATATGATCTCTATGTTCCCAGTCGTATCCGCCAACATTTGCCCCCAGACCAGGAACAGATGCGGGTGTTTA
CCCATTTGCAAGTTCGTGAAGATCAAATGGTATTGTTTGGCTTTGCAGTGGTAGCGGAGCGCGATTTGTTTCGTCAACTG
ATTGCAGTGAGCGGGATTGGCCCCCAGCTGGCATTGGCCTTAATTGATACTTTAGGATTACAGGATCTTGTGCAAGCGAT
TGTTAATAGCAACACAAAGATGCTTGTTAAGACGCCTGGTGTTGGAGCCAAGACGGCTGAACGTATTGCCTTAGAGCTGC
GATCCAAGTTGGCCGAATGGCGAGATCAGGCAGGTTTAAAGACCTTACCGAGTGCTGGGCCGATTGATAGTGTGCAAGAA
GATGTGGAGATGACGCTTTTAGCTTTGGGATATACCAGCCAAGAAGTGATGCGGGCGTTACAGGCCGTAGGGCAAAATAC
AGCGTTGGCCAAAAATTCAGATACTGAAGCCTGGATTCGAGAAGCTATTGCGTGGCTAAGTCAATAG

Upstream 100 bases:

>100_bases
TGCAATGGTATCAAGAACATAATCAGGATGATATATACCTTGCTAAAGCGAATTGTGCGGGCGGTATTTTAGAAGGGCTC
CGTCATTTTAGTTATGTCCA

Downstream 100 bases:

>100_bases
CTTAGAAACTGGTATGGCTTGGGATGCTTTTCTAGAGTGTTTTGGAGACATATCTTATGTCAGCTAGAGCAGTTTTGCCA
GGATAAAACAACATAATTCT

Product: Holliday junction DNA helicase RuvA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 208; Mature: 208

Protein sequence:

>208_residues
MIGFLQGYVVSLQQTSTHRTLLTLDVNQVGYDLYVPSRIRQHLPPDQEQMRVFTHLQVREDQMVLFGFAVVAERDLFRQL
IAVSGIGPQLALALIDTLGLQDLVQAIVNSNTKMLVKTPGVGAKTAERIALELRSKLAEWRDQAGLKTLPSAGPIDSVQE
DVEMTLLALGYTSQEVMRALQAVGQNTALAKNSDTEAWIREAIAWLSQ

Sequences:

>Translated_208_residues
MIGFLQGYVVSLQQTSTHRTLLTLDVNQVGYDLYVPSRIRQHLPPDQEQMRVFTHLQVREDQMVLFGFAVVAERDLFRQL
IAVSGIGPQLALALIDTLGLQDLVQAIVNSNTKMLVKTPGVGAKTAERIALELRSKLAEWRDQAGLKTLPSAGPIDSVQE
DVEMTLLALGYTSQEVMRALQAVGQNTALAKNSDTEAWIREAIAWLSQ
>Mature_208_residues
MIGFLQGYVVSLQQTSTHRTLLTLDVNQVGYDLYVPSRIRQHLPPDQEQMRVFTHLQVREDQMVLFGFAVVAERDLFRQL
IAVSGIGPQLALALIDTLGLQDLVQAIVNSNTKMLVKTPGVGAKTAERIALELRSKLAEWRDQAGLKTLPSAGPIDSVQE
DVEMTLLALGYTSQEVMRALQAVGQNTALAKNSDTEAWIREAIAWLSQ

Specific function: The ruvA-ruvB complex in the presence of ATP renatures cruciform structure in supercoiled DNA with palindromic sequence, indicating that it may promote strand exchange reactions in homologous recombination. RuvAB is an helicase that mediates the Holliday

COG id: COG0632

COG function: function code L; Holliday junction resolvasome, DNA-binding subunit

Gene ontology:
GO:0000166: Holliday junction ATP-dependent DNA helicase ruvA
GO:0003677: Holliday junction ATP-dependent DNA helicase ruvA
GO:0003678: Holliday junction ATP-dependent DNA helicase ruvA
GO:0004386: Holliday junction ATP-dependent DNA helicase ruvA
GO:0005524: Holliday junction ATP-dependent DNA helicase ruvA
GO:0006281: Holliday junction ATP-dependent DNA helicase ruvA
GO:0006310: Holliday junction ATP-dependent DNA helicase ruvA
GO:0006974: Holliday junction ATP-dependent DNA helicase ruvA
GO:0009378: Holliday junction ATP-dependent DNA helicase ruvA
GO:0009379: Holliday junction ATP-dependent DNA helicase ruvA
GO:0009432: Holliday junction ATP-dependent DNA helicase ruvA
GO:0016787: Holliday junction ATP-dependent DNA helicase ruvA

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ruvA family

Homologues:

Organism=Escherichia coli, GI1788168, Length=209, Percent_Identity=33.9712918660287, Blast_Score=120, Evalue=6e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RUVA_ACAM1 (B0CAE7)

Other databases:

- EMBL:   CP000828
- RefSeq:   YP_001517198.1
- ProteinModelPortal:   B0CAE7
- SMR:   B0CAE7
- GeneID:   5681691
- GenomeReviews:   CP000828_GR
- KEGG:   amr:AM1_2883
- HOGENOM:   HBG635309
- OMA:   THLQVRE
- ProtClustDB:   PRK00116
- BioCyc:   AMAR329726:AM1_2883-MONOMER
- HAMAP:   MF_00031
- InterPro:   IPR011114
- InterPro:   IPR013849
- InterPro:   IPR003583
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR000085
- InterPro:   IPR010994
- Gene3D:   G3DSA:2.40.50.140
- SMART:   SM00278
- TIGRFAMs:   TIGR00084

Pfam domain/function: PF07499 RuvA_C; PF01330 RuvA_N; SSF50249 Nucleic_acid_OB; SSF47781 RuvA_2_like; SSF46929 RuvA_C-like

EC number: =3.6.4.12

Molecular weight: Translated: 23012; Mature: 23012

Theoretical pI: Translated: 5.27; Mature: 5.27

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIGFLQGYVVSLQQTSTHRTLLTLDVNQVGYDLYVPSRIRQHLPPDQEQMRVFTHLQVRE
CCCCHHHHHHHHHHCCCCEEEEEEEHHHCCEEEECHHHHHHCCCCCHHHHHHHHHHHHCC
DQMVLFGFAVVAERDLFRQLIAVSGIGPQLALALIDTLGLQDLVQAIVNSNTKMLVKTPG
CCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCHHHHHHHHHCCCCEEEEECCC
VGAKTAERIALELRSKLAEWRDQAGLKTLPSAGPIDSVQEDVEMTLLALGYTSQEVMRAL
CCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHH
QAVGQNTALAKNSDTEAWIREAIAWLSQ
HHHCCCCCCCCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MIGFLQGYVVSLQQTSTHRTLLTLDVNQVGYDLYVPSRIRQHLPPDQEQMRVFTHLQVRE
CCCCHHHHHHHHHHCCCCEEEEEEEHHHCCEEEECHHHHHHCCCCCHHHHHHHHHHHHCC
DQMVLFGFAVVAERDLFRQLIAVSGIGPQLALALIDTLGLQDLVQAIVNSNTKMLVKTPG
CCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCHHHHHHHHHCCCCEEEEECCC
VGAKTAERIALELRSKLAEWRDQAGLKTLPSAGPIDSVQEDVEMTLLALGYTSQEVMRAL
CCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHH
QAVGQNTALAKNSDTEAWIREAIAWLSQ
HHHCCCCCCCCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA