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The map label for this gene is rph

Identifier: 158335996

GI number: 158335996

Start: 2896650

End: 2897369

Strand: Reverse

Name: rph

Synonym: AM1_2854

Alternate gene names: 158335996

Gene position: 2897369-2896650 (Counterclockwise)

Preceding gene: 158335997

Following gene: 158335993

Centisome position: 44.55

GC content: 54.03

Gene sequence:

>720_bases
ATGACTTGGCAACGTCCTGATGGTCGTCAACCGGATCAGCTTCGACCGATTCGGTTTCACCGAGAGTATACCCACTTTGC
CGCTGGATCGGTACTGGCAGAATGTGGACAGACGAAAGTTCTGTGTACGGTCTCTGTGCAGCCCGGTGTCCCCCGGTTTT
TAGAAGATACGGGCCAGGGGTGGTTAACGGCAGAATATCGCATGCTCCCTGGCTCAACCCCAGAACGGCAGCGCCGAGAA
CTGATGAAATTATCGGGACGCACTCAAGAAATTCAGCGGTTAATCGGTCGGAGTTTGCGGGCAGCCCTCGATTTACAGGC
TCTAGGAGAACGAACCTTGGTGGTGGATGCCGATGTCTTACAAGCCGATGCAGGAACGCGCACGACATCCATTACAGGTG
GATTTGTGGCCCTTGTGGATGCGGTGAATCAGCTGGTGAGCAAGGGAGAAATTCCCCAATCCCCCATTCGTCATCATGTC
GCCGCCGTATCGGTTGGGTTGCTAGAAGGTCAGCCCTTTCTAGATCTCAACTACCCAGAAGATGTCGGTGCGGCGGTGGA
TTTGAATGTCGTGGCAACGGAACAGCTAGAGCTGCTAGAAGTTCAAGGGACGGCGGAAGAAGGCACTTTGCCTCGTCCCC
AGCTCAATCAAATGTTAGACCTGGCAGAAATTGGCATTCAACAGTTAATCGAGGCCCAGCGTCAGGCGTTAGCCTCGTAA

Upstream 100 bases:

>100_bases
GGGTGATCTAGAGTATGCTGGTTGGGATTTAAACTGGCTCACCCACAATACTTAGGTCAGTGGTGGATGCCAGCCCCTGT
GTAGTTGAGAATAAGCATAC

Downstream 100 bases:

>100_bases
GCGCCTAAGCCTTGACTTGGGTGAAATGGTCGACAATGCGGTCTAGCCCCACGGAGTGGGACGCTTTGCAGAGAAGACGA
TCGCCCGGCTGAATATGCTG

Product: ribonuclease PH

Products: NA

Alternate protein names: RNase PH; tRNA nucleotidyltransferase

Number of amino acids: Translated: 239; Mature: 238

Protein sequence:

>239_residues
MTWQRPDGRQPDQLRPIRFHREYTHFAAGSVLAECGQTKVLCTVSVQPGVPRFLEDTGQGWLTAEYRMLPGSTPERQRRE
LMKLSGRTQEIQRLIGRSLRAALDLQALGERTLVVDADVLQADAGTRTTSITGGFVALVDAVNQLVSKGEIPQSPIRHHV
AAVSVGLLEGQPFLDLNYPEDVGAAVDLNVVATEQLELLEVQGTAEEGTLPRPQLNQMLDLAEIGIQQLIEAQRQALAS

Sequences:

>Translated_239_residues
MTWQRPDGRQPDQLRPIRFHREYTHFAAGSVLAECGQTKVLCTVSVQPGVPRFLEDTGQGWLTAEYRMLPGSTPERQRRE
LMKLSGRTQEIQRLIGRSLRAALDLQALGERTLVVDADVLQADAGTRTTSITGGFVALVDAVNQLVSKGEIPQSPIRHHV
AAVSVGLLEGQPFLDLNYPEDVGAAVDLNVVATEQLELLEVQGTAEEGTLPRPQLNQMLDLAEIGIQQLIEAQRQALAS
>Mature_238_residues
TWQRPDGRQPDQLRPIRFHREYTHFAAGSVLAECGQTKVLCTVSVQPGVPRFLEDTGQGWLTAEYRMLPGSTPERQRREL
MKLSGRTQEIQRLIGRSLRAALDLQALGERTLVVDADVLQADAGTRTTSITGGFVALVDAVNQLVSKGEIPQSPIRHHVA
AVSVGLLEGQPFLDLNYPEDVGAAVDLNVVATEQLELLEVQGTAEEGTLPRPQLNQMLDLAEIGIQQLIEAQRQALAS

Specific function: Phosphorolytic exoribonuclease that removes nucleotide residues following the -CCA terminus of tRNA and adds nucleotides to the ends of RNA molecules by using nucleoside diphosphates as substrates

COG id: COG0689

COG function: function code J; RNase PH

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNase PH family

Homologues:

Organism=Escherichia coli, GI157672248, Length=204, Percent_Identity=58.8235294117647, Blast_Score=235, Evalue=2e-63,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RNPH_ACAM1 (B0CAB9)

Other databases:

- EMBL:   CP000828
- RefSeq:   YP_001517170.1
- ProteinModelPortal:   B0CAB9
- SMR:   B0CAB9
- GeneID:   5681662
- GenomeReviews:   CP000828_GR
- KEGG:   amr:AM1_2854
- HOGENOM:   HBG737187
- OMA:   MLPRATG
- ProtClustDB:   PRK00173
- BioCyc:   AMAR329726:AM1_2854-MONOMER
- HAMAP:   MF_00564
- InterPro:   IPR001247
- InterPro:   IPR015847
- InterPro:   IPR020568
- InterPro:   IPR002381
- InterPro:   IPR018336
- TIGRFAMs:   TIGR01966

Pfam domain/function: PF01138 RNase_PH; PF03725 RNase_PH_C; SSF55666 3_ExoRNase; SSF54211 Ribosomal_S5_D2-typ_fold

EC number: =2.7.7.56

Molecular weight: Translated: 26193; Mature: 26062

Theoretical pI: Translated: 4.84; Mature: 4.84

Prosite motif: PS01277 RIBONUCLEASE_PH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTWQRPDGRQPDQLRPIRFHREYTHFAAGSVLAECGQTKVLCTVSVQPGVPRFLEDTGQG
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCHHHHHCCCC
WLTAEYRMLPGSTPERQRRELMKLSGRTQEIQRLIGRSLRAALDLQALGERTLVVDADVL
EEEEEEEECCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEEEHHHH
QADAGTRTTSITGGFVALVDAVNQLVSKGEIPQSPIRHHVAAVSVGLLEGQPFLDLNYPE
HCCCCCCEEECCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCEEECCCCC
DVGAAVDLNVVATEQLELLEVQGTAEEGTLPRPQLNQMLDLAEIGIQQLIEAQRQALAS
CCCCEEEEEEEECCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TWQRPDGRQPDQLRPIRFHREYTHFAAGSVLAECGQTKVLCTVSVQPGVPRFLEDTGQG
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCHHHHHCCCC
WLTAEYRMLPGSTPERQRRELMKLSGRTQEIQRLIGRSLRAALDLQALGERTLVVDADVL
EEEEEEEECCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEEEHHHH
QADAGTRTTSITGGFVALVDAVNQLVSKGEIPQSPIRHHVAAVSVGLLEGQPFLDLNYPE
HCCCCCCEEECCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCEEECCCCC
DVGAAVDLNVVATEQLELLEVQGTAEEGTLPRPQLNQMLDLAEIGIQQLIEAQRQALAS
CCCCEEEEEEEECCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA