Click here to switch to the map view.

The map label for this gene is frhB

Identifier: 158335991

GI number: 158335991

Start: 2891216

End: 2892415

Strand: Reverse

Name: frhB

Synonym: AM1_2849

Alternate gene names: NA

Gene position: 2892415-2891216 (Counterclockwise)

Preceding gene: 158335992

Following gene: 158335990

Centisome position: 44.47

GC content: 50.5

Gene sequence:

>1200_bases
TTGACTATGACTGCGGTACAACCCCATAAAAAAGCCAAAGCCCTCAAGTCTACGAGTCGCCGACCGGCTAAAGAACTCTG
TAGTGAATGTGGTCTGTGCGATACCTACTACATTCACTATGTAAAAGAAGCCTGTGCCTTTTTAAATCAGCAGGTAGCTG
AATTAGAAGAAAAAGCCCACGGTCGTAGCCGTGACTTGGATAACGAAGATGATTGGTACTTTGGGGTCAGCAACCAAATG
ATGACGGCCCGCAAGGTCGATCCCATTCCGGGAGCGCAATGGACAGGCATTGTCAGCACCATCGGTATGGAAATGCTGAA
GCAGGGCAAAGTGGACGGCGTTGTCTGTATCCAAAATACCGATGAAGATCGGTTCCAACCCAAGCCGATTATTGCTCGTA
CTCCAGAAGAGGTGTTGGCAGCTCGGGTGAATAAGCCCACCCTCTCCCCCAATCTCTCGGTATTGGAGCAGATTGAACAG
TCGGGCCTGAAGCGTTTGTGTGTGATTGGCGTGGGCTGTCAAACCCAGGCTTTGCGGTCTGTTCAGGACAGCTTAGGACT
AGAAAAGCTGTATGTTCTGGGAACCCCTTGTGTAGACAATGTGAATCGAGCCGGGTTACAGACTTTTTTAGAAACCACGA
GCCGATCTCCAGATACGGTTGTCCACTACGAATTTATGCAGGATTTCCGCATCCACTTTAAGCATGAAGATGGCTCGATT
GAAACGGTGCCGTTCTTTGGCCTGAAGACCAATAAGCTCAAGGATATTTTTGCGCCGTCCTGCATGAGCTGCTTCGACTA
CACCAATTCCCTGGCGGATCTTGTGGTGGGCTATATGGGGTCGCCCTATGGCTGGCAGTGGCTTGTGGTGCGTAATGAAA
CGGGGCAAGAAATGCTGGATCTGGTGATGGATCAGCTGGAAACCCAGCCCGTGATTTCCGAAGGCGATCGCCATGCCGCA
GTGCAAAATAGTATCCCTGCTTATGATAAAGGGGTGACCTTGCCCATGTGGGCGGCGAAGCTGATGGGCGTGGTGATCGA
CAAGATTGGTCCCCGAGGTTTAGAATATGCGCGGTTCTCCATTGATTCCCACTTTACCCGGAACTACCTGTATGTGAAGC
GCAACTATCCTGATAAGGTAGATGCCCATGTACCTGAGTACGCCAAGCGCATTGTCAGTCAATATGACTTACCGGACTAA

Upstream 100 bases:

>100_bases
AGGGGGCTTGGGTCGCGGTCCCGAAACTGTTTACAATCGGAATGCGATATCACCATTCAGTTCGGTCTCCTGCCCTTACT
TCGCAGGACTGAACCCTAAT

Downstream 100 bases:

>100_bases
ATTTGCATGACCCGACCGTCTGACGCTCTATCCCGGTCGCCCAATTTGAAAAATTTGGGTGGTGCATTCCGCATTGTGGG
CTGGATCAGTTTCTGGATCA

Product: coenzyme F420 hydrogenase beta subunit

Products: reduced coenzyme F420

Alternate protein names: NA

Number of amino acids: Translated: 399; Mature: 398

Protein sequence:

>399_residues
MTMTAVQPHKKAKALKSTSRRPAKELCSECGLCDTYYIHYVKEACAFLNQQVAELEEKAHGRSRDLDNEDDWYFGVSNQM
MTARKVDPIPGAQWTGIVSTIGMEMLKQGKVDGVVCIQNTDEDRFQPKPIIARTPEEVLAARVNKPTLSPNLSVLEQIEQ
SGLKRLCVIGVGCQTQALRSVQDSLGLEKLYVLGTPCVDNVNRAGLQTFLETTSRSPDTVVHYEFMQDFRIHFKHEDGSI
ETVPFFGLKTNKLKDIFAPSCMSCFDYTNSLADLVVGYMGSPYGWQWLVVRNETGQEMLDLVMDQLETQPVISEGDRHAA
VQNSIPAYDKGVTLPMWAAKLMGVVIDKIGPRGLEYARFSIDSHFTRNYLYVKRNYPDKVDAHVPEYAKRIVSQYDLPD

Sequences:

>Translated_399_residues
MTMTAVQPHKKAKALKSTSRRPAKELCSECGLCDTYYIHYVKEACAFLNQQVAELEEKAHGRSRDLDNEDDWYFGVSNQM
MTARKVDPIPGAQWTGIVSTIGMEMLKQGKVDGVVCIQNTDEDRFQPKPIIARTPEEVLAARVNKPTLSPNLSVLEQIEQ
SGLKRLCVIGVGCQTQALRSVQDSLGLEKLYVLGTPCVDNVNRAGLQTFLETTSRSPDTVVHYEFMQDFRIHFKHEDGSI
ETVPFFGLKTNKLKDIFAPSCMSCFDYTNSLADLVVGYMGSPYGWQWLVVRNETGQEMLDLVMDQLETQPVISEGDRHAA
VQNSIPAYDKGVTLPMWAAKLMGVVIDKIGPRGLEYARFSIDSHFTRNYLYVKRNYPDKVDAHVPEYAKRIVSQYDLPD
>Mature_398_residues
TMTAVQPHKKAKALKSTSRRPAKELCSECGLCDTYYIHYVKEACAFLNQQVAELEEKAHGRSRDLDNEDDWYFGVSNQMM
TARKVDPIPGAQWTGIVSTIGMEMLKQGKVDGVVCIQNTDEDRFQPKPIIARTPEEVLAARVNKPTLSPNLSVLEQIEQS
GLKRLCVIGVGCQTQALRSVQDSLGLEKLYVLGTPCVDNVNRAGLQTFLETTSRSPDTVVHYEFMQDFRIHFKHEDGSIE
TVPFFGLKTNKLKDIFAPSCMSCFDYTNSLADLVVGYMGSPYGWQWLVVRNETGQEMLDLVMDQLETQPVISEGDRHAAV
QNSIPAYDKGVTLPMWAAKLMGVVIDKIGPRGLEYARFSIDSHFTRNYLYVKRNYPDKVDAHVPEYAKRIVSQYDLPD

Specific function: Unknown

COG id: COG1035

COG function: function code C; Coenzyme F420-reducing hydrogenase, beta subunit

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007516
- InterPro:   IPR007525 [H]

Pfam domain/function: PF04432 FrhB_FdhB_C; PF04422 FrhB_FdhB_N [H]

EC number: 1.12.98.1

Molecular weight: Translated: 44951; Mature: 44820

Theoretical pI: Translated: 6.06; Mature: 6.06

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTMTAVQPHKKAKALKSTSRRPAKELCSECGLCDTYYIHYVKEACAFLNQQVAELEEKAH
CCCCCCCCHHHHHHHHHHCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHC
GRSRDLDNEDDWYFGVSNQMMTARKVDPIPGAQWTGIVSTIGMEMLKQGKVDGVVCIQNT
CCCCCCCCCCCEEEECCCCHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEECC
DEDRFQPKPIIARTPEEVLAARVNKPTLSPNLSVLEQIEQSGLKRLCVIGVGCQTQALRS
CCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCEEEEEECCCHHHHHHH
VQDSLGLEKLYVLGTPCVDNVNRAGLQTFLETTSRSPDTVVHYEFMQDFRIHFKHEDGSI
HHHHCCCEEEEEECCCCCCCCHHHHHHHHHHHHCCCCCCEEEHHHHHCCEEEEEECCCCE
ETVPFFGLKTNKLKDIFAPSCMSCFDYTNSLADLVVGYMGSPYGWQWLVVRNETGQEMLD
EEEEEECCCCCCHHHHHCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCHHHHHH
LVMDQLETQPVISEGDRHAAVQNSIPAYDKGVTLPMWAAKLMGVVIDKIGPRGLEYARFS
HHHHHHCCCCCCCCCCCHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHEEE
IDSHFTRNYLYVKRNYPDKVDAHVPEYAKRIVSQYDLPD
CCCCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHCCCCC
>Mature Secondary Structure 
TMTAVQPHKKAKALKSTSRRPAKELCSECGLCDTYYIHYVKEACAFLNQQVAELEEKAH
CCCCCCCHHHHHHHHHHCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHC
GRSRDLDNEDDWYFGVSNQMMTARKVDPIPGAQWTGIVSTIGMEMLKQGKVDGVVCIQNT
CCCCCCCCCCCEEEECCCCHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEECC
DEDRFQPKPIIARTPEEVLAARVNKPTLSPNLSVLEQIEQSGLKRLCVIGVGCQTQALRS
CCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCEEEEEECCCHHHHHHH
VQDSLGLEKLYVLGTPCVDNVNRAGLQTFLETTSRSPDTVVHYEFMQDFRIHFKHEDGSI
HHHHCCCEEEEEECCCCCCCCHHHHHHHHHHHHCCCCCCEEEHHHHHCCEEEEEECCCCE
ETVPFFGLKTNKLKDIFAPSCMSCFDYTNSLADLVVGYMGSPYGWQWLVVRNETGQEMLD
EEEEEECCCCCCHHHHHCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCHHHHHH
LVMDQLETQPVISEGDRHAAVQNSIPAYDKGVTLPMWAAKLMGVVIDKIGPRGLEYARFS
HHHHHHCCCCCCCCCCCHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHEEE
IDSHFTRNYLYVKRNYPDKVDAHVPEYAKRIVSQYDLPD
CCCCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: H2; coenzyme F420

Specific reaction: H2 + coenzyme F420 = reduced coenzyme F420

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11759840 [H]