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The map label for this gene is pknA [H]

Identifier: 158335934

GI number: 158335934

Start: 2823773

End: 2824738

Strand: Direct

Name: pknA [H]

Synonym: AM1_2792

Alternate gene names: 158335934

Gene position: 2823773-2824738 (Clockwise)

Preceding gene: 158335932

Following gene: 158335935

Centisome position: 43.42

GC content: 48.86

Gene sequence:

>966_bases
ATGGTTAGCACTTGTATCGCACCAACACCAACTTTACTCAATAATCGCTATCAGATTGTTCGCATCCTTGAGGACGGACC
CTGGAGCATTGCCTGGTTGGCTGAGGATACACAGCTTCCTTCTCGTCGGCAATGCGTTATTCGCCAGGTCAAGCCCCTGA
CCGCCGATGCAGACATTCAAAAACAGCTTTGCGATCGCATTCAAGCAGAAATCGAAATCTGGCAAAAGCTAGGCGGCATC
CATGACCAAATTCCTTGGTTATCCAGTTTTGCCGATAGCACTGGCCAAGTCTATCTCATGGAAGAATGGGTACCAGGCCT
CACTCTCACCCAAACCGTGCAAGAACAAGGCGCATTAGACGAAGCGACTGTTCAACAGATCATGACCAAGCTTCTGTCTT
TGGTCGATTATCTACACCAAAATCAAATCGTCCACTGTACCTTGGAACCCAACAATATTATGTTGCGACAAGACGACAAT
AAGCCTGTCCTTCAGTATGGCGGCATGTTAAATCAGGTCATTGCCAACACATTTCTGGACAACAACATTCGGCCCACCCC
TTGGATATCCACATCTGGCTTCTTACCCCATGAGCAGGTCATCCTCGATCAGCACTACTACTCCAGCAACCTTTACAGTT
TGGGTCTGATCGGCATTTATTTGTTGACCGGGCAAGATCCTTACGATCTGTTTAACCTCAACACCGGGTCCTGTGATTGG
AATACCCAGACCTTAGACCCAGATTCAGCCTTTGCTGCCATTTTGAACAAAGCCATTGCTCGATTCCCAGGAGAGCGCTT
CCTAACCGCTCGGCAAATGATGCAGGCTTTGGCAGCACCCGGGCAATTCGTCCAACAATCCCTCTCGGTTCACTCCACGC
TAGTGGAGCAAATGGGAGCCTATTTAGATTCATGCCTCCTGAGTCTAAATCCCTCTGAGCCACCCCTTAATTCCTATTCG
GGATAG

Upstream 100 bases:

>100_bases
CTTTGTGATTAGCTTCAGGTGATTTCTGTGAGATGTGTCACTGAGTAACCCCAATGGAACTGGCATTGTCTAGACATAGA
TGTTCTGGGGAATACCCAAG

Downstream 100 bases:

>100_bases
TCAAGTCTCTCCTCCGAACATCTTAAGTTTGCTTTGGGTGATACCCATCACGTTAACAGCGTGATAGCTCTCAGTCGTGG
TCAGTGATGATTCTCATCGC

Product: serine/threonine protein kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 321; Mature: 321

Protein sequence:

>321_residues
MVSTCIAPTPTLLNNRYQIVRILEDGPWSIAWLAEDTQLPSRRQCVIRQVKPLTADADIQKQLCDRIQAEIEIWQKLGGI
HDQIPWLSSFADSTGQVYLMEEWVPGLTLTQTVQEQGALDEATVQQIMTKLLSLVDYLHQNQIVHCTLEPNNIMLRQDDN
KPVLQYGGMLNQVIANTFLDNNIRPTPWISTSGFLPHEQVILDQHYYSSNLYSLGLIGIYLLTGQDPYDLFNLNTGSCDW
NTQTLDPDSAFAAILNKAIARFPGERFLTARQMMQALAAPGQFVQQSLSVHSTLVEQMGAYLDSCLLSLNPSEPPLNSYS
G

Sequences:

>Translated_321_residues
MVSTCIAPTPTLLNNRYQIVRILEDGPWSIAWLAEDTQLPSRRQCVIRQVKPLTADADIQKQLCDRIQAEIEIWQKLGGI
HDQIPWLSSFADSTGQVYLMEEWVPGLTLTQTVQEQGALDEATVQQIMTKLLSLVDYLHQNQIVHCTLEPNNIMLRQDDN
KPVLQYGGMLNQVIANTFLDNNIRPTPWISTSGFLPHEQVILDQHYYSSNLYSLGLIGIYLLTGQDPYDLFNLNTGSCDW
NTQTLDPDSAFAAILNKAIARFPGERFLTARQMMQALAAPGQFVQQSLSVHSTLVEQMGAYLDSCLLSLNPSEPPLNSYS
G
>Mature_321_residues
MVSTCIAPTPTLLNNRYQIVRILEDGPWSIAWLAEDTQLPSRRQCVIRQVKPLTADADIQKQLCDRIQAEIEIWQKLGGI
HDQIPWLSSFADSTGQVYLMEEWVPGLTLTQTVQEQGALDEATVQQIMTKLLSLVDYLHQNQIVHCTLEPNNIMLRQDDN
KPVLQYGGMLNQVIANTFLDNNIRPTPWISTSGFLPHEQVILDQHYYSSNLYSLGLIGIYLLTGQDPYDLFNLNTGSCDW
NTQTLDPDSAFAAILNKAIARFPGERFLTARQMMQALAAPGQFVQQSLSVHSTLVEQMGAYLDSCLLSLNPSEPPLNSYS
G

Specific function: Probably required for both normal cellular growth and differentiation. Inactivation of pknA leads to colonies that appear light green and rough in the absence of combined nitrogen [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 protein kinase domain [H]

Homologues:

Organism=Saccharomyces cerevisiae, GI6325104, Length=176, Percent_Identity=28.9772727272727, Blast_Score=65, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011009
- InterPro:   IPR000719
- InterPro:   IPR017441
- InterPro:   IPR017442
- InterPro:   IPR008271 [H]

Pfam domain/function: PF00069 Pkinase [H]

EC number: =2.7.11.1 [H]

Molecular weight: Translated: 36025; Mature: 36025

Theoretical pI: Translated: 4.40; Mature: 4.40

Prosite motif: PS50011 PROTEIN_KINASE_DOM

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVSTCIAPTPTLLNNRYQIVRILEDGPWSIAWLAEDTQLPSRRQCVIRQVKPLTADADIQ
CCCCCCCCCHHHHCCCEEEEEEEECCCEEEEEEECCCCCCHHHHHHHHHHCCCCCCHHHH
KQLCDRIQAEIEIWQKLGGIHDQIPWLSSFADSTGQVYLMEEWVPGLTLTQTVQEQGALD
HHHHHHHHHHHHHHHHHCCCHHCCCHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHCCCC
EATVQQIMTKLLSLVDYLHQNQIVHCTLEPNNIMLRQDDNKPVLQYGGMLNQVIANTFLD
HHHHHHHHHHHHHHHHHHHCCCEEEEEECCCEEEEEECCCCCHHHHHHHHHHHHHHHHHC
NNIRPTPWISTSGFLPHEQVILDQHYYSSNLYSLGLIGIYLLTGQDPYDLFNLNTGSCDW
CCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHEEEEEEECCCCCCCEEECCCCCCCC
NTQTLDPDSAFAAILNKAIARFPGERFLTARQMMQALAAPGQFVQQSLSVHSTLVEQMGA
CCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
YLDSCLLSLNPSEPPLNSYSG
HHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure
MVSTCIAPTPTLLNNRYQIVRILEDGPWSIAWLAEDTQLPSRRQCVIRQVKPLTADADIQ
CCCCCCCCCHHHHCCCEEEEEEEECCCEEEEEEECCCCCCHHHHHHHHHHCCCCCCHHHH
KQLCDRIQAEIEIWQKLGGIHDQIPWLSSFADSTGQVYLMEEWVPGLTLTQTVQEQGALD
HHHHHHHHHHHHHHHHHCCCHHCCCHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHCCCC
EATVQQIMTKLLSLVDYLHQNQIVHCTLEPNNIMLRQDDNKPVLQYGGMLNQVIANTFLD
HHHHHHHHHHHHHHHHHHHCCCEEEEEECCCEEEEEECCCCCHHHHHHHHHHHHHHHHHC
NNIRPTPWISTSGFLPHEQVILDQHYYSSNLYSLGLIGIYLLTGQDPYDLFNLNTGSCDW
CCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHEEEEEEECCCCCCCEEECCCCCCCC
NTQTLDPDSAFAAILNKAIARFPGERFLTARQMMQALAAPGQFVQQSLSVHSTLVEQMGA
CCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
YLDSCLLSLNPSEPPLNSYSG
HHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7505448; 11759840 [H]