Click here to switch to the map view.

The map label for this gene is glcF [H]

Identifier: 158335885

GI number: 158335885

Start: 2777864

End: 2779267

Strand: Reverse

Name: glcF [H]

Synonym: AM1_2743

Alternate gene names: 158335885

Gene position: 2779267-2777864 (Counterclockwise)

Preceding gene: 158335886

Following gene: 158335884

Centisome position: 42.73

GC content: 52.49

Gene sequence:

>1404_bases
ATGACTACTTCTGAATCACCCCATTCTCTGCAGGTGACGGCTGCGACAGTGGATTGGTCCGAGCCGCCAGCGCAGCCCTC
TACATCCCCTGAACAGTCCTTGATCGATAGCTGTGTCCATTGTGGATTTTGTCTATCCACCTGTCCTAGTTACCGTGTGA
TTGGCAAAGAAACCGATTCGCCTCGGGGCCGGGTCTATTTAATGAACGGCCTCAATCAAGGTGACTTTACTTTGACGGAT
GCTGCTGTGGGCCATTTTGATACCTGCTTAGGCTGTTTGGCCTGTGTCAGCACTTGTCCGTCAGGGGTTGAGTATGACCA
GCTAATCACCTCAACCCGAGCCCAGATTGAACAGAATTATCAACGCAGTCTGCCCCAGCGACTCCTGCGGAAAATGCTGT
TCTCGTTACTGCCCTATCCTCAGCGATTGCGACGACTACTAGGTCCCCTACAGCTTTACCAAAAACTACTAGGGTTGCAA
AGTATGTTGCGCCAGTCAGGGCTGTTGGCACGCGTCTTGCCAAAGTCTCTCGTCGCTATGGAGTCGCTGCTTCCTCCCAT
TGAAGCCCAAGCCTTTACGGATCCAGAATCGGAGATCTTTCCCGCCCAGGGAAAACGTCGCTATCGGGTGGGCTTGATTT
TGGGCTGTATCCAGCGTCTCTTTTTATCTGAAGTCAATCAAGCGACCATTCGGGTGTTGACGGCGAATGGCTGCGAAGTG
GTGGTTCCCAGAAGTCAGGGGTGCTGTGCTGCCTTGCCCCACCACCAAGGCCAAGTGGATCAGGCCCAAGACTTAGCCCG
ACAGATGATGGATAGTTTTGCCGATTATGACTTGGATGCTGTGATCGTCAATGCCTCCGGCTGTGGTCATACCCTCAAGG
AATATGGCCACATCTTGCAGGACGATCCAGCCTATCGCGATCGCGCCCAAGCCTTTGCCCACCAAGTCAAAGATGTTCAG
GAATTTCTGGATGAAGTGGGCTTAACGACCCCCTTGTCTCCTCTGCAGCCAGAGCCATTGGCTGTCGTCTATCAAGATGC
CTGTCATATGCTCCATGGCCAAAAAATCCAGGCGCAACCCCGTCGCCTCCTGCAGCAAATTCCGGGTTTAGAACTCCGCG
AACCCCGTGATGCTTTTCTCTGTTGTGGCAGTGCTGGGGTCTATAACATTCTCCAACCCGACATTGCGGAAGAGTTGGGG
CAGCAAAAAGTGCAGAATCTTACCCAGACGGGGGCCACTTTAATTGCCTCGGCTAATGTGGGTTGTACCATGCAAATTCG
CAAACATTTAGATCAACAAGACCATACCACTCCTGTGCTCCATCCCATGCAGCTGTTAGATTGCTCTATTCGGGGTGTTT
CTTGGTCAACGGCTGTAGGGAATGCTCAGGACAGACCCCTATAA

Upstream 100 bases:

>100_bases
GGGGATATCCTGGCAACGCCCTCGCCAGTATGAAACAGCTCAAACAGCAATTTGACCCTCACAATATTTTGAATCCTGAT
CGTTTCGTTGGTGGAATTTA

Downstream 100 bases:

>100_bases
ACTGGCCTGATTGCGGCTGCCCTCCTTTCTATGTATCTCATCCACAAACTCGCATCTTGGTTAGAAACCCATTGGGCTGT
GCCAGCCTATGCGGGCTGGG

Product: glycolate oxidase subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 467; Mature: 466

Protein sequence:

>467_residues
MTTSESPHSLQVTAATVDWSEPPAQPSTSPEQSLIDSCVHCGFCLSTCPSYRVIGKETDSPRGRVYLMNGLNQGDFTLTD
AAVGHFDTCLGCLACVSTCPSGVEYDQLITSTRAQIEQNYQRSLPQRLLRKMLFSLLPYPQRLRRLLGPLQLYQKLLGLQ
SMLRQSGLLARVLPKSLVAMESLLPPIEAQAFTDPESEIFPAQGKRRYRVGLILGCIQRLFLSEVNQATIRVLTANGCEV
VVPRSQGCCAALPHHQGQVDQAQDLARQMMDSFADYDLDAVIVNASGCGHTLKEYGHILQDDPAYRDRAQAFAHQVKDVQ
EFLDEVGLTTPLSPLQPEPLAVVYQDACHMLHGQKIQAQPRRLLQQIPGLELREPRDAFLCCGSAGVYNILQPDIAEELG
QQKVQNLTQTGATLIASANVGCTMQIRKHLDQQDHTTPVLHPMQLLDCSIRGVSWSTAVGNAQDRPL

Sequences:

>Translated_467_residues
MTTSESPHSLQVTAATVDWSEPPAQPSTSPEQSLIDSCVHCGFCLSTCPSYRVIGKETDSPRGRVYLMNGLNQGDFTLTD
AAVGHFDTCLGCLACVSTCPSGVEYDQLITSTRAQIEQNYQRSLPQRLLRKMLFSLLPYPQRLRRLLGPLQLYQKLLGLQ
SMLRQSGLLARVLPKSLVAMESLLPPIEAQAFTDPESEIFPAQGKRRYRVGLILGCIQRLFLSEVNQATIRVLTANGCEV
VVPRSQGCCAALPHHQGQVDQAQDLARQMMDSFADYDLDAVIVNASGCGHTLKEYGHILQDDPAYRDRAQAFAHQVKDVQ
EFLDEVGLTTPLSPLQPEPLAVVYQDACHMLHGQKIQAQPRRLLQQIPGLELREPRDAFLCCGSAGVYNILQPDIAEELG
QQKVQNLTQTGATLIASANVGCTMQIRKHLDQQDHTTPVLHPMQLLDCSIRGVSWSTAVGNAQDRPL
>Mature_466_residues
TTSESPHSLQVTAATVDWSEPPAQPSTSPEQSLIDSCVHCGFCLSTCPSYRVIGKETDSPRGRVYLMNGLNQGDFTLTDA
AVGHFDTCLGCLACVSTCPSGVEYDQLITSTRAQIEQNYQRSLPQRLLRKMLFSLLPYPQRLRRLLGPLQLYQKLLGLQS
MLRQSGLLARVLPKSLVAMESLLPPIEAQAFTDPESEIFPAQGKRRYRVGLILGCIQRLFLSEVNQATIRVLTANGCEVV
VPRSQGCCAALPHHQGQVDQAQDLARQMMDSFADYDLDAVIVNASGCGHTLKEYGHILQDDPAYRDRAQAFAHQVKDVQE
FLDEVGLTTPLSPLQPEPLAVVYQDACHMLHGQKIQAQPRRLLQQIPGLELREPRDAFLCCGSAGVYNILQPDIAEELGQ
QKVQNLTQTGATLIASANVGCTMQIRKHLDQQDHTTPVLHPMQLLDCSIRGVSWSTAVGNAQDRPL

Specific function: Unknown

COG id: COG0247

COG function: function code C; Fe-S oxidoreductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI48994913, Length=420, Percent_Identity=33.3333333333333, Blast_Score=214, Evalue=1e-56,
Organism=Escherichia coli, GI1786497, Length=238, Percent_Identity=25.6302521008403, Blast_Score=104, Evalue=1e-23,
Organism=Escherichia coli, GI1788576, Length=426, Percent_Identity=25.5868544600939, Blast_Score=87, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR004017
- InterPro:   IPR012285
- InterPro:   IPR012257 [H]

Pfam domain/function: PF02754 CCG [H]

EC number: NA

Molecular weight: Translated: 51466; Mature: 51334

Theoretical pI: Translated: 6.31; Mature: 6.31

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.9 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
3.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTSESPHSLQVTAATVDWSEPPAQPSTSPEQSLIDSCVHCGFCLSTCPSYRVIGKETDS
CCCCCCCCEEEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEECCCCCC
PRGRVYLMNGLNQGDFTLTDAAVGHFDTCLGCLACVSTCPSGVEYDQLITSTRAQIEQNY
CCCEEEEECCCCCCCEEEHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHH
QRSLPQRLLRKMLFSLLPYPQRLRRLLGPLQLYQKLLGLQSMLRQSGLLARVLPKSLVAM
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
ESLLPPIEAQAFTDPESEIFPAQGKRRYRVGLILGCIQRLFLSEVNQATIRVLTANGCEV
HHHCCCCCHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCEE
VVPRSQGCCAALPHHQGQVDQAQDLARQMMDSFADYDLDAVIVNASGCGHTLKEYGHILQ
EEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHCHHHC
DDPAYRDRAQAFAHQVKDVQEFLDEVGLTTPLSPLQPEPLAVVYQDACHMLHGQKIQAQP
CCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHH
RRLLQQIPGLELREPRDAFLCCGSAGVYNILQPDIAEELGQQKVQNLTQTGATLIASANV
HHHHHHCCCCCCCCCCCCEEECCCCCCHHHCCHHHHHHHHHHHHHHHHHHCHHEEEECCC
GCTMQIRKHLDQQDHTTPVLHPMQLLDCSIRGVSWSTAVGNAQDRPL
CCHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCEECCCCCCCCCCCC
>Mature Secondary Structure 
TTSESPHSLQVTAATVDWSEPPAQPSTSPEQSLIDSCVHCGFCLSTCPSYRVIGKETDS
CCCCCCCEEEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEECCCCCC
PRGRVYLMNGLNQGDFTLTDAAVGHFDTCLGCLACVSTCPSGVEYDQLITSTRAQIEQNY
CCCEEEEECCCCCCCEEEHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHH
QRSLPQRLLRKMLFSLLPYPQRLRRLLGPLQLYQKLLGLQSMLRQSGLLARVLPKSLVAM
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
ESLLPPIEAQAFTDPESEIFPAQGKRRYRVGLILGCIQRLFLSEVNQATIRVLTANGCEV
HHHCCCCCHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCEE
VVPRSQGCCAALPHHQGQVDQAQDLARQMMDSFADYDLDAVIVNASGCGHTLKEYGHILQ
EEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHCHHHC
DDPAYRDRAQAFAHQVKDVQEFLDEVGLTTPLSPLQPEPLAVVYQDACHMLHGQKIQAQP
CCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHH
RRLLQQIPGLELREPRDAFLCCGSAGVYNILQPDIAEELGQQKVQNLTQTGATLIASANV
HHHHHHCCCCCCCCCCCCEEECCCCCCHHHCCHHHHHHHHHHHHHHHHHHCHHEEEECCC
GCTMQIRKHLDQQDHTTPVLHPMQLLDCSIRGVSWSTAVGNAQDRPL
CCHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCEECCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969504; 9384377 [H]