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The map label for this gene is rffG [C]

Identifier: 158335848

GI number: 158335848

Start: 2737620

End: 2738654

Strand: Direct

Name: rffG [C]

Synonym: AM1_2706

Alternate gene names: 158335848

Gene position: 2737620-2738654 (Clockwise)

Preceding gene: 158335847

Following gene: 158335849

Centisome position: 42.09

GC content: 41.55

Gene sequence:

>1035_bases
ATGTCTTCAACAACAGCTACCGAGAACACCATTAGTCCTGGTCAACGTGTTTTAGTAACTGGAGCAACTGGCTATACAGG
CAGCACCTTAGTCCAAAAACTGCTAAACCAGAATGTCGAGGTTGTTGCAATAGCTCGTCCATCCTCTGATTTAAGCCGTT
GGCAAGATGCGCCCATTCGATGGATTCAAGGAGATGTTTTTGATCCAAAGCTCATTCAGGAGGCGATGGAAGGAGTGAAC
TATATATTTCATATGGTCACCCCATTTCGGGAAGCAAAGTCATCTGATGATGTTTATTACAATGTCCATGTTAAAAGTAC
ACAGTTACTAGCGAACGAAGCCTTGAAGCAACCCGACTTCAAGCGCTTTGTTCATATCTCGACAATCGGAGTTCATGGAC
ATATCGAAAATCCTCCTGCTGATGAGACCTATAGAACTTCTCCTGGAGATTTATACCAATCCACCAAATTAGAAGGTGAA
ATATGGATCAAGCAATTTGGTGCGGAAACTGGGCTCCCCATAACCATCATTAGACCTGCAGGCATTATCGGTCCTGGCGA
AAAGAGACTGCTAAAAATCTACAAGATGGTTTGTTCTGGTTTAGTACCTGCCATTGGTAATGGAGGCAACCTACTGCATT
TAATTCACGTTGATGATTTGACCAACTGTTTTTTACTAGCATCCCAACATCCAAATGCAGTGAATGAAATCTTCATCTGT
GGAAATCAAGACTCGATTTCTTTCAAAGAAATGGTCAATCTCATCTCCAAGTACTACCAGAAAAAAGCCACACTTCTACA
ACTCCCTTCCGCCCCCTTATTTCTTCTGGGAGATATTCTTGAGTTTGTTTGTAAACCTTTAAATATCGAGCCTCCCATAT
ACCGGAGAAGACTTGCCTTTTACACCAAAGATCGGTCATTCAATGTTAACAAGCTTAAATCAAAACTAGGGTTTGTTCCC
CAGCATTCAAATGAGCAGTGTATTACTGAATTGGCACAGTGGTATTTAGAAGAAGGATGGACAAACATTAGCTAA

Upstream 100 bases:

>100_bases
TGGGGCTCTCCAGTGATTACCAGATTTCATAAAGTGCATTAATCTATTGATGACTCAATTTCATCATTAATCTAACTATC
AACATCTATATCACTACTGT

Downstream 100 bases:

>100_bases
TTTATACGAGTAGATAAAATTTACTGTTAGAGGTGACATTACGAAACTATAATTCTTAGGTATCAATCTAATTAATGTAA
CACAATATTGTTCTAGTGAT

Product: NAD-dependent epimerase/dehydratase

Products: dTDP-4-dehydro-6-deoxy-D-glucose; H2O [C]

Alternate protein names: NA

Number of amino acids: Translated: 344; Mature: 343

Protein sequence:

>344_residues
MSSTTATENTISPGQRVLVTGATGYTGSTLVQKLLNQNVEVVAIARPSSDLSRWQDAPIRWIQGDVFDPKLIQEAMEGVN
YIFHMVTPFREAKSSDDVYYNVHVKSTQLLANEALKQPDFKRFVHISTIGVHGHIENPPADETYRTSPGDLYQSTKLEGE
IWIKQFGAETGLPITIIRPAGIIGPGEKRLLKIYKMVCSGLVPAIGNGGNLLHLIHVDDLTNCFLLASQHPNAVNEIFIC
GNQDSISFKEMVNLISKYYQKKATLLQLPSAPLFLLGDILEFVCKPLNIEPPIYRRRLAFYTKDRSFNVNKLKSKLGFVP
QHSNEQCITELAQWYLEEGWTNIS

Sequences:

>Translated_344_residues
MSSTTATENTISPGQRVLVTGATGYTGSTLVQKLLNQNVEVVAIARPSSDLSRWQDAPIRWIQGDVFDPKLIQEAMEGVN
YIFHMVTPFREAKSSDDVYYNVHVKSTQLLANEALKQPDFKRFVHISTIGVHGHIENPPADETYRTSPGDLYQSTKLEGE
IWIKQFGAETGLPITIIRPAGIIGPGEKRLLKIYKMVCSGLVPAIGNGGNLLHLIHVDDLTNCFLLASQHPNAVNEIFIC
GNQDSISFKEMVNLISKYYQKKATLLQLPSAPLFLLGDILEFVCKPLNIEPPIYRRRLAFYTKDRSFNVNKLKSKLGFVP
QHSNEQCITELAQWYLEEGWTNIS
>Mature_343_residues
SSTTATENTISPGQRVLVTGATGYTGSTLVQKLLNQNVEVVAIARPSSDLSRWQDAPIRWIQGDVFDPKLIQEAMEGVNY
IFHMVTPFREAKSSDDVYYNVHVKSTQLLANEALKQPDFKRFVHISTIGVHGHIENPPADETYRTSPGDLYQSTKLEGEI
WIKQFGAETGLPITIIRPAGIIGPGEKRLLKIYKMVCSGLVPAIGNGGNLLHLIHVDDLTNCFLLASQHPNAVNEIFICG
NQDSISFKEMVNLISKYYQKKATLLQLPSAPLFLLGDILEFVCKPLNIEPPIYRRRLAFYTKDRSFNVNKLKSKLGFVPQ
HSNEQCITELAQWYLEEGWTNIS

Specific function: Putative nucleotide sugar epimerase/dehydrogenase [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Homo sapiens, GI239745448, Length=350, Percent_Identity=23.4285714285714, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI310132178, Length=350, Percent_Identity=23.4285714285714, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI310113012, Length=350, Percent_Identity=23.4285714285714, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI193211614, Length=226, Percent_Identity=27.4336283185841, Blast_Score=72, Evalue=1e-12,
Organism=Homo sapiens, GI8393516, Length=226, Percent_Identity=27.4336283185841, Blast_Score=72, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: 4.2.1.46 [C]

Molecular weight: Translated: 38549; Mature: 38417

Theoretical pI: Translated: 7.26; Mature: 7.26

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSTTATENTISPGQRVLVTGATGYTGSTLVQKLLNQNVEVVAIARPSSDLSRWQDAPIR
CCCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHCCCCEEEEEECCCHHHHHHCCCCCE
WIQGDVFDPKLIQEAMEGVNYIFHMVTPFREAKSSDDVYYNVHVKSTQLLANEALKQPDF
EEECCCCCHHHHHHHHHHHHHHHHHHCCHHHCCCCCCEEEEEEECHHHHHHHHHHCCCCH
KRFVHISTIGVHGHIENPPADETYRTSPGDLYQSTKLEGEIWIKQFGAETGLPITIIRPA
HHEEEEEEEEEECCCCCCCCCCCCCCCCCHHHHHCCCCHHHHHHHHCCCCCCCEEEECCC
GIIGPGEKRLLKIYKMVCSGLVPAIGNGGNLLHLIHVDDLTNCFLLASQHPNAVNEIFIC
CCCCCCHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEECCCCCEEEEECCCCCCCCEEEEE
GNQDSISFKEMVNLISKYYQKKATLLQLPSAPLFLLGDILEFVCKPLNIEPPIYRRRLAF
CCCCCCCHHHHHHHHHHHHHHHHHEEECCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHE
YTKDRSFNVNKLKSKLGFVPQHSNEQCITELAQWYLEEGWTNIS
EECCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
SSTTATENTISPGQRVLVTGATGYTGSTLVQKLLNQNVEVVAIARPSSDLSRWQDAPIR
CCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHCCCCEEEEEECCCHHHHHHCCCCCE
WIQGDVFDPKLIQEAMEGVNYIFHMVTPFREAKSSDDVYYNVHVKSTQLLANEALKQPDF
EEECCCCCHHHHHHHHHHHHHHHHHHCCHHHCCCCCCEEEEEEECHHHHHHHHHHCCCCH
KRFVHISTIGVHGHIENPPADETYRTSPGDLYQSTKLEGEIWIKQFGAETGLPITIIRPA
HHEEEEEEEEEECCCCCCCCCCCCCCCCCHHHHHCCCCHHHHHHHHCCCCCCCEEEECCC
GIIGPGEKRLLKIYKMVCSGLVPAIGNGGNLLHLIHVDDLTNCFLLASQHPNAVNEIFIC
CCCCCCHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEECCCCCEEEEECCCCCCCCEEEEE
GNQDSISFKEMVNLISKYYQKKATLLQLPSAPLFLLGDILEFVCKPLNIEPPIYRRRLAF
CCCCCCCHHHHHHHHHHHHHHHHHEEECCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHE
YTKDRSFNVNKLKSKLGFVPQHSNEQCITELAQWYLEEGWTNIS
EECCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NAD. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.093 {dTDPglucose}} [C]

Substrates: dTDPglucose [C]

Specific reaction: dTDPglucose --> dTDP-4-dehydro-6-deoxy-D-glucose + H2O [C]

General reaction: Elimination (of H2O C-O bond cleavage [C]

Inhibitor: p-Chloromercuribenzoate; TMP [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9163424 [H]