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The map label for this gene is wecG [H]

Identifier: 158335826

GI number: 158335826

Start: 2719737

End: 2721143

Strand: Direct

Name: wecG [H]

Synonym: AM1_2682

Alternate gene names: 158335826

Gene position: 2719737-2721143 (Clockwise)

Preceding gene: 158335825

Following gene: 158335827

Centisome position: 41.82

GC content: 46.48

Gene sequence:

>1407_bases
TTGTGGTATCAACCGGTGTATGACGTTTCTTCAGGGAACGTTCTTCAAAATGAAGTGCTTTTACGATGGCGGGATACCCA
AGGGCTGATCCATCGGCCGAAAGAGTTTATGCCAGTGGTTCAAAATGCGGATGCTGAGCCATGGTTAGATCGCTATGTGA
CGGGACAGGCCATTGCCCAATTAAAGCAACACGCTCACCTTAAGCTTTCTATTAATTTATCAAAGCGCATTGTCGAAGAT
CGCTTTATCGCCGAAGATATTTACGATCTGCTGGTCAACCACAGCGTTCAACCGCACCAACTGCACTTTGAAGTATCAGA
AGCGAGTCTGGCCAAAAATTTCCAGGATTCAGTTGCTTTAATCCGAGATCTAAAAGAACTCGGATGCTCCGTCACCTTAG
ACAATTTTGCTAATCAACATCTCACCTTTCTGCAGTGGGAAAAGCTAGGGGTTGATGCCGTTAAAATCGACCCGTCTTTG
ATTCGCGACCTGAGACAAGATTCGCCCCAAACGCGATTGGCCAAAGCCATCATAGAAACCAGTACCGCCTTGGCACAACC
TGCGATCGCAACTTCCGTAGACGCTTACGCCACCTCTCGTCACTTAGTGAATCTAAGCTTTAAGTCCGCCCAGGGATATC
ACTTCAAGCCTCCTAGCGATCACCCTTGGCTCACAAACACAGTTGATATTTTAGGGGTTCCCATTGATAACGTGACCCAA
GACGAGTTGCTCCAGGAACTGAAATCGGGCATCGTTTTTACTCCCAATGTTGACCATCTCATGAACCTGAAGCAGGATGA
AGAGTTTCGAGCTGCTTACAACATCGCCGACTACAAAATTTGCGATAGCCAGATTCTTTACTTTTCCTCCCATTTCCTTG
GCTCACCGATCAAAGAAAAGATTTCAGGATCAGATTTATTTCCCGCCTTTTACACCTATCACAAAGATAATCCAGACATC
ACCATTTTCCTGTTAGGTGCTGCGCCAGGCGTAGCCGCTCAAGCGCAACAAAACATCAATGCCCATGTGGGTCGTGATAT
CGTCGTTGGTTCTTACTCTCCCTCCTATGGTTTTGAAAACAACCCCGAGGAATGTGCAGAGATTATCGAACGGATTCATC
ACTCTGGAGCAACGGTTCTCGCCATTGGAGTCGGATCGCCCAAGCAAGAGCGATGGATTTATCGGTATCGTCAACAGCTA
GCAGAGTCTGTAAATATTATTTTTGCCATAGGCGGCACTTTAGATTTTGAGGCTGGTACTCAAAAACGGGCTCCAAAGCT
CATCAGCACCATGGGCGTTGAGTGGCTCTTTCGCCTGATGGGTGAACCGAAACGCCTCTGGAAACGTTATTTAATTAACG
ACCTGCCATTCTTGAAATTAATGCTGCATCAAAAATTTGATCGCTAA

Upstream 100 bases:

>100_bases
TCATGTTTCGATAGCTTATCTCTCTCTACAACCTATGCTGGCTAAGCCTCAAACCATTCTGCCTTCTCCACAACTTGCAA
TTAATCCCCGTGGGTTTGAG

Downstream 100 bases:

>100_bases
TGCGTCCTACAAGCTGCTTCTAACAGCGATTTAATCTATAACTCCCATTCTTACCGCTGAGTGAGCAGTGGGTTGTCTTG
TACGGCTGCACAAGCCATGA

Product: glycosyl transferase WecB/TagA/CpsF

Products: C55-PP-GlcNAc-ManNAcA; UDP [C]

Alternate protein names: UDP-ManNAcA transferase [H]

Number of amino acids: Translated: 468; Mature: 468

Protein sequence:

>468_residues
MWYQPVYDVSSGNVLQNEVLLRWRDTQGLIHRPKEFMPVVQNADAEPWLDRYVTGQAIAQLKQHAHLKLSINLSKRIVED
RFIAEDIYDLLVNHSVQPHQLHFEVSEASLAKNFQDSVALIRDLKELGCSVTLDNFANQHLTFLQWEKLGVDAVKIDPSL
IRDLRQDSPQTRLAKAIIETSTALAQPAIATSVDAYATSRHLVNLSFKSAQGYHFKPPSDHPWLTNTVDILGVPIDNVTQ
DELLQELKSGIVFTPNVDHLMNLKQDEEFRAAYNIADYKICDSQILYFSSHFLGSPIKEKISGSDLFPAFYTYHKDNPDI
TIFLLGAAPGVAAQAQQNINAHVGRDIVVGSYSPSYGFENNPEECAEIIERIHHSGATVLAIGVGSPKQERWIYRYRQQL
AESVNIIFAIGGTLDFEAGTQKRAPKLISTMGVEWLFRLMGEPKRLWKRYLINDLPFLKLMLHQKFDR

Sequences:

>Translated_468_residues
MWYQPVYDVSSGNVLQNEVLLRWRDTQGLIHRPKEFMPVVQNADAEPWLDRYVTGQAIAQLKQHAHLKLSINLSKRIVED
RFIAEDIYDLLVNHSVQPHQLHFEVSEASLAKNFQDSVALIRDLKELGCSVTLDNFANQHLTFLQWEKLGVDAVKIDPSL
IRDLRQDSPQTRLAKAIIETSTALAQPAIATSVDAYATSRHLVNLSFKSAQGYHFKPPSDHPWLTNTVDILGVPIDNVTQ
DELLQELKSGIVFTPNVDHLMNLKQDEEFRAAYNIADYKICDSQILYFSSHFLGSPIKEKISGSDLFPAFYTYHKDNPDI
TIFLLGAAPGVAAQAQQNINAHVGRDIVVGSYSPSYGFENNPEECAEIIERIHHSGATVLAIGVGSPKQERWIYRYRQQL
AESVNIIFAIGGTLDFEAGTQKRAPKLISTMGVEWLFRLMGEPKRLWKRYLINDLPFLKLMLHQKFDR
>Mature_468_residues
MWYQPVYDVSSGNVLQNEVLLRWRDTQGLIHRPKEFMPVVQNADAEPWLDRYVTGQAIAQLKQHAHLKLSINLSKRIVED
RFIAEDIYDLLVNHSVQPHQLHFEVSEASLAKNFQDSVALIRDLKELGCSVTLDNFANQHLTFLQWEKLGVDAVKIDPSL
IRDLRQDSPQTRLAKAIIETSTALAQPAIATSVDAYATSRHLVNLSFKSAQGYHFKPPSDHPWLTNTVDILGVPIDNVTQ
DELLQELKSGIVFTPNVDHLMNLKQDEEFRAAYNIADYKICDSQILYFSSHFLGSPIKEKISGSDLFPAFYTYHKDNPDI
TIFLLGAAPGVAAQAQQNINAHVGRDIVVGSYSPSYGFENNPEECAEIIERIHHSGATVLAIGVGSPKQERWIYRYRQQL
AESVNIIFAIGGTLDFEAGTQKRAPKLISTMGVEWLFRLMGEPKRLWKRYLINDLPFLKLMLHQKFDR

Specific function: Catalyzes the synthesis of Und-PP-GlcNAc-ManNAcA (Lipid II), the second lipid-linked intermediate involved in ECA synthesis [H]

COG id: COG1922

COG function: function code M; Teichoic acid biosynthesis proteins

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 26 family [H]

Homologues:

Organism=Escherichia coli, GI2367289, Length=203, Percent_Identity=30.0492610837438, Blast_Score=98, Evalue=1e-21,
Organism=Escherichia coli, GI1790496, Length=225, Percent_Identity=27.1111111111111, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1788849, Length=208, Percent_Identity=26.4423076923077, Blast_Score=78, Evalue=9e-16,
Organism=Escherichia coli, GI1787541, Length=220, Percent_Identity=24.0909090909091, Blast_Score=76, Evalue=4e-15,
Organism=Escherichia coli, GI87081743, Length=217, Percent_Identity=25.8064516129032, Blast_Score=76, Evalue=6e-15,
Organism=Escherichia coli, GI226510982, Length=225, Percent_Identity=27.1111111111111, Blast_Score=75, Evalue=1e-14,
Organism=Escherichia coli, GI87081921, Length=221, Percent_Identity=23.9819004524887, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1787055, Length=223, Percent_Identity=21.52466367713, Blast_Score=66, Evalue=4e-12,
Organism=Escherichia coli, GI1786507, Length=222, Percent_Identity=22.0720720720721, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI87082096, Length=206, Percent_Identity=22.3300970873786, Blast_Score=62, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023085
- InterPro:   IPR004629 [H]

Pfam domain/function: PF03808 Glyco_tran_WecB [H]

EC number: 2.4.1.- [C]

Molecular weight: Translated: 53090; Mature: 53090

Theoretical pI: Translated: 6.46; Mature: 6.46

Prosite motif: PS50883 EAL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MWYQPVYDVSSGNVLQNEVLLRWRDTQGLIHRPKEFMPVVQNADAEPWLDRYVTGQAIAQ
CCCCCCEECCCCCEEECCEEEEEECCCCHHHCCHHHHHHHHCCCCCHHHHHHHHHHHHHH
LKQHAHLKLSINLSKRIVEDRFIAEDIYDLLVNHSVQPHQLHFEVSEASLAKNFQDSVAL
HHHCCEEEEEECHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEHHHHHHHHHHHHHHH
IRDLKELGCSVTLDNFANQHLTFLQWEKLGVDAVKIDPSLIRDLRQDSPQTRLAKAIIET
HHHHHHCCCEEEEHHHCCCCEEEEEHHHCCCCEEEECHHHHHHHHHCCCHHHHHHHHHHH
STALAQPAIATSVDAYATSRHLVNLSFKSAQGYHFKPPSDHPWLTNTVDILGVPIDNVTQ
HHHHHCCHHHHHHHHHHCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCEEEECCCCCCCH
DELLQELKSGIVFTPNVDHLMNLKQDEEFRAAYNIADYKICDSQILYFSSHFLGSPIKEK
HHHHHHHHCCEEECCCHHHHHCCCCCHHHHHHHCCCCEEECCCHHEEHHHHHCCCCHHHH
ISGSDLFPAFYTYHKDNPDITIFLLGAAPGVAAQAQQNINAHVGRDIVVGSYSPSYGFEN
CCCCCCCHHHHEEECCCCCEEEEEEECCCCCHHHHHHCCCHHCCCEEEEECCCCCCCCCC
NPEECAEIIERIHHSGATVLAIGVGSPKQERWIYRYRQQLAESVNIIFAIGGTLDFEAGT
CHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHHCCEEEEEECCEEECCCCC
QKRAPKLISTMGVEWLFRLMGEPKRLWKRYLINDLPFLKLMLHQKFDR
CHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCHHHHHHHHHHCCC
>Mature Secondary Structure
MWYQPVYDVSSGNVLQNEVLLRWRDTQGLIHRPKEFMPVVQNADAEPWLDRYVTGQAIAQ
CCCCCCEECCCCCEEECCEEEEEECCCCHHHCCHHHHHHHHCCCCCHHHHHHHHHHHHHH
LKQHAHLKLSINLSKRIVEDRFIAEDIYDLLVNHSVQPHQLHFEVSEASLAKNFQDSVAL
HHHCCEEEEEECHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEHHHHHHHHHHHHHHH
IRDLKELGCSVTLDNFANQHLTFLQWEKLGVDAVKIDPSLIRDLRQDSPQTRLAKAIIET
HHHHHHCCCEEEEHHHCCCCEEEEEHHHCCCCEEEECHHHHHHHHHCCCHHHHHHHHHHH
STALAQPAIATSVDAYATSRHLVNLSFKSAQGYHFKPPSDHPWLTNTVDILGVPIDNVTQ
HHHHHCCHHHHHHHHHHCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCEEEECCCCCCCH
DELLQELKSGIVFTPNVDHLMNLKQDEEFRAAYNIADYKICDSQILYFSSHFLGSPIKEK
HHHHHHHHCCEEECCCHHHHHCCCCCHHHHHHHCCCCEEECCCHHEEHHHHHCCCCHHHH
ISGSDLFPAFYTYHKDNPDITIFLLGAAPGVAAQAQQNINAHVGRDIVVGSYSPSYGFEN
CCCCCCCHHHHEEECCCCCEEEEEEECCCCCHHHHHHCCCHHCCCEEEEECCCCCCCCCC
NPEECAEIIERIHHSGATVLAIGVGSPKQERWIYRYRQQLAESVNIIFAIGGTLDFEAGT
CHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHHCCEEEEEECCEEECCCCC
QKRAPKLISTMGVEWLFRLMGEPKRLWKRYLINDLPFLKLMLHQKFDR
CHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: UDP-N-acetyl-D-mannosaminuronic-acid; undecaprenyl-N-acetyl-alpha-D-glucosaminyl-pyrophosphate [C]

Specific reaction: UDP-N-acetyl-D-mannosaminuronic-acid + undecaprenyl-N-acetyl-alpha-D-glucosaminyl-pyrophosphate = C55-PP-GlcNAc-ManNAcA + UDP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA