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The map label for this gene is gidA

Identifier: 158335804

GI number: 158335804

Start: 2697270

End: 2698667

Strand: Direct

Name: gidA

Synonym: AM1_2655

Alternate gene names: 158335804

Gene position: 2697270-2698667 (Clockwise)

Preceding gene: 158335803

Following gene: 158335805

Centisome position: 41.47

GC content: 53.51

Gene sequence:

>1398_bases
ATGGACCATCAACAACCCATTCAGGTGATTGGGGGAGGACTCGCAGGGACTGAGGCCGCTTGGCAAATTGCCCAAGCGGG
ATGGCCCGTGATTTTGCATGAGATGCGTTCTGTGGAGGAAGAGCGGCTGACGCCCGCTCACCACACAGACCAGCTGGCTG
AGTTGGTCTGTAGTAATTCCTTTGGTGCACAAGCTAGCGATCGCGCCGCCGGATTGCTCCATGCTGAACTGCGCCAGCTC
GGTTCCATCATTATTCGCAAAGCCGATGAACATCAGGTCCCTGCTGGGGGAGCACTAGCAGTAGACCGAGGCGTTTTTAG
CCGAAATTTGACAGAAACCTTGGCTGAGCATCCTAATGTCGAATTGCGCCGGGGTGAAGTTCCCACCTTACCCATCGATG
GCACTGTCGTATTGACCTCTGGACCATTGACCAGTGCCGCTTTAACGGAGAGTTTGCACCAATTTACCGGCCAAGATTAT
CTCAGCTTTTTTGATGCTGCCAGTCCCATCATCGTCGGTGAATCCATTAACCGAGATGTGGCATTTCTGGCCTCTCGCTA
CGACCGAGGCGAAGCGGCCTATCTCAACTGCCCCATGAATCGGGAGCAATATTTAGCCTTTTGGCAGGCCCTCTGTGCAG
CGGAACAGGCTGAACTCAAAGACTTTGAAAAAGAAACCGCTAAATTCTTCGAGGGCTGCTTACCCATTGAAGAAATGGCC
CGCCGGGGTGAAGACACAATGCGCTATGGCCCCCTCAAGCCTGTAGGTCTATTCGATGCCCGCTATGGCGACTTTAAAGC
ACCGGAAAACCAGAAGTATCGTCCCTATGCCGTGGCTCAACTCCGCCAGGAAGATAAGGCAGGCAAGCTCTGGAATCTAG
TCGGTTTCCAGACTAATCTGCGCTGGGGTGAACAAAAGCGGGTATTCCAGATGATTCCAGGATTGGAATCGGCAGAGTTT
GTACGCATGGGGGTGATGCATCGCAATACGTTTATCAATGCGCCGGAATTGCTTCATCCCACCTTACAGTTTAAGCAACG
GCCGACGTTACTGGCAGCGGGACAGTTGATCGGTACGGAGGGCTATACAGCGGCGACGGCAGGAGGGTGGCTAGCAGGCA
CAAATGCAGTGCGCTTAGCTCAGGGGCTGAACCCTGTGGTTCTACCGACAACCACCATGAGTGGTTCGCTGTTTGAATTT
ATCAGTTCAGCTACACCGAAGCATTTTCAGCCGATGCCCCCTAACTTTGGCATTATTCCTGATCTGCCGCAGCGAGTTCG
GAATAAGCGTGAGCGTTATGGAGTCTATCGCGATCGCGCCTTAGCCGATCTAACCGCCTGGATTGCGGAACCTTCTCTCT
TTCGGCAAACGGTTGCGGCTAACTCGGCCAGTATGTAA

Upstream 100 bases:

>100_bases
CACCTTGCTGCCAGGAGAGGATACCCGCCAATCTTCAATCAGCCCTTAAGAACCAAGACTGCGACTATCCTGGTAAAGGA
GACGCTTGCAGACGAACACC

Downstream 100 bases:

>100_bases
TTCAGCCAGACGCTCAACGAACCCTTGTAATGTATTGATTTGAATACGACATAACGCTACTTGAAACAGCCCAATACACG
AGAACCGTTGGTGAAGAATA

Product: tRNA (uracil-5-)-methyltransferase Gid

Products: NA

Alternate protein names: Folate-dependent tRNA (uracil-5-)-methyltransferase; Folate-dependent tRNA(M-5-U54)-methyltransferase

Number of amino acids: Translated: 465; Mature: 465

Protein sequence:

>465_residues
MDHQQPIQVIGGGLAGTEAAWQIAQAGWPVILHEMRSVEEERLTPAHHTDQLAELVCSNSFGAQASDRAAGLLHAELRQL
GSIIIRKADEHQVPAGGALAVDRGVFSRNLTETLAEHPNVELRRGEVPTLPIDGTVVLTSGPLTSAALTESLHQFTGQDY
LSFFDAASPIIVGESINRDVAFLASRYDRGEAAYLNCPMNREQYLAFWQALCAAEQAELKDFEKETAKFFEGCLPIEEMA
RRGEDTMRYGPLKPVGLFDARYGDFKAPENQKYRPYAVAQLRQEDKAGKLWNLVGFQTNLRWGEQKRVFQMIPGLESAEF
VRMGVMHRNTFINAPELLHPTLQFKQRPTLLAAGQLIGTEGYTAATAGGWLAGTNAVRLAQGLNPVVLPTTTMSGSLFEF
ISSATPKHFQPMPPNFGIIPDLPQRVRNKRERYGVYRDRALADLTAWIAEPSLFRQTVAANSASM

Sequences:

>Translated_465_residues
MDHQQPIQVIGGGLAGTEAAWQIAQAGWPVILHEMRSVEEERLTPAHHTDQLAELVCSNSFGAQASDRAAGLLHAELRQL
GSIIIRKADEHQVPAGGALAVDRGVFSRNLTETLAEHPNVELRRGEVPTLPIDGTVVLTSGPLTSAALTESLHQFTGQDY
LSFFDAASPIIVGESINRDVAFLASRYDRGEAAYLNCPMNREQYLAFWQALCAAEQAELKDFEKETAKFFEGCLPIEEMA
RRGEDTMRYGPLKPVGLFDARYGDFKAPENQKYRPYAVAQLRQEDKAGKLWNLVGFQTNLRWGEQKRVFQMIPGLESAEF
VRMGVMHRNTFINAPELLHPTLQFKQRPTLLAAGQLIGTEGYTAATAGGWLAGTNAVRLAQGLNPVVLPTTTMSGSLFEF
ISSATPKHFQPMPPNFGIIPDLPQRVRNKRERYGVYRDRALADLTAWIAEPSLFRQTVAANSASM
>Mature_465_residues
MDHQQPIQVIGGGLAGTEAAWQIAQAGWPVILHEMRSVEEERLTPAHHTDQLAELVCSNSFGAQASDRAAGLLHAELRQL
GSIIIRKADEHQVPAGGALAVDRGVFSRNLTETLAEHPNVELRRGEVPTLPIDGTVVLTSGPLTSAALTESLHQFTGQDY
LSFFDAASPIIVGESINRDVAFLASRYDRGEAAYLNCPMNREQYLAFWQALCAAEQAELKDFEKETAKFFEGCLPIEEMA
RRGEDTMRYGPLKPVGLFDARYGDFKAPENQKYRPYAVAQLRQEDKAGKLWNLVGFQTNLRWGEQKRVFQMIPGLESAEF
VRMGVMHRNTFINAPELLHPTLQFKQRPTLLAAGQLIGTEGYTAATAGGWLAGTNAVRLAQGLNPVVLPTTTMSGSLFEF
ISSATPKHFQPMPPNFGIIPDLPQRVRNKRERYGVYRDRALADLTAWIAEPSLFRQTVAANSASM

Specific function: Catalyzes the folate-dependent formation of 5-methyl- uridine at position 54 (M-5-U54) in all tRNAs

COG id: COG1206

COG function: function code J; NAD(FAD)-utilizing enzyme possibly involved in translation

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MnmG family. TrmFO subfamily

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRMFO_ACAM1 (B0C6V8)

Other databases:

- EMBL:   CP000828
- RefSeq:   YP_001516978.1
- ProteinModelPortal:   B0C6V8
- GeneID:   5681467
- GenomeReviews:   CP000828_GR
- KEGG:   amr:AM1_2655
- HOGENOM:   HBG285041
- OMA:   FEGCLPI
- ProtClustDB:   PRK05335
- BioCyc:   AMAR329726:AM1_2655-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01037
- InterPro:   IPR004417
- InterPro:   IPR002218
- TIGRFAMs:   TIGR00137

Pfam domain/function: PF01134 GIDA

EC number: =2.1.1.74

Molecular weight: Translated: 51375; Mature: 51375

Theoretical pI: Translated: 6.30; Mature: 6.30

Prosite motif: PS01280 GIDA_1; PS01281 GIDA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDHQQPIQVIGGGLAGTEAAWQIAQAGWPVILHEMRSVEEERLTPAHHTDQLAELVCSNS
CCCCCCHHHHCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCC
FGAQASDRAAGLLHAELRQLGSIIIRKADEHQVPAGGALAVDRGVFSRNLTETLAEHPNV
CCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEECCCHHHHHHHHHHHHCCCC
ELRRGEVPTLPIDGTVVLTSGPLTSAALTESLHQFTGQDYLSFFDAASPIIVGESINRDV
EEECCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHCCHHHHHHHHCCCCEEECCCCCCHH
AFLASRYDRGEAAYLNCPMNREQYLAFWQALCAAEQAELKDFEKETAKFFEGCLPIEEMA
HHHHHHCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
RRGEDTMRYGPLKPVGLFDARYGDFKAPENQKYRPYAVAQLRQEDKAGKLWNLVGFQTNL
HCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCEEEECCCCCCC
RWGEQKRVFQMIPGLESAEFVRMGVMHRNTFINAPELLHPTLQFKQRPTLLAAGQLIGTE
CCCHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCEEECCCEECCC
GYTAATAGGWLAGTNAVRLAQGLNPVVLPTTTMSGSLFEFISSATPKHFQPMPPNFGIIP
CCCEECCCCCCCCCCHHHHHHCCCCEEEECCCCCHHHHHHHHCCCCCCCCCCCCCCCCCC
DLPQRVRNKRERYGVYRDRALADLTAWIAEPSLFRQTVAANSASM
CCHHHHHHHHHHCCHHHHHHHHHHHHHHHCHHHHHHHHHHCCCCC
>Mature Secondary Structure
MDHQQPIQVIGGGLAGTEAAWQIAQAGWPVILHEMRSVEEERLTPAHHTDQLAELVCSNS
CCCCCCHHHHCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCC
FGAQASDRAAGLLHAELRQLGSIIIRKADEHQVPAGGALAVDRGVFSRNLTETLAEHPNV
CCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEECCCHHHHHHHHHHHHCCCC
ELRRGEVPTLPIDGTVVLTSGPLTSAALTESLHQFTGQDYLSFFDAASPIIVGESINRDV
EEECCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHCCHHHHHHHHCCCCEEECCCCCCHH
AFLASRYDRGEAAYLNCPMNREQYLAFWQALCAAEQAELKDFEKETAKFFEGCLPIEEMA
HHHHHHCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
RRGEDTMRYGPLKPVGLFDARYGDFKAPENQKYRPYAVAQLRQEDKAGKLWNLVGFQTNL
HCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCEEEECCCCCCC
RWGEQKRVFQMIPGLESAEFVRMGVMHRNTFINAPELLHPTLQFKQRPTLLAAGQLIGTE
CCCHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCEEECCCEECCC
GYTAATAGGWLAGTNAVRLAQGLNPVVLPTTTMSGSLFEFISSATPKHFQPMPPNFGIIP
CCCEECCCCCCCCCCHHHHHHCCCCEEEECCCCCHHHHHHHHCCCCCCCCCCCCCCCCCC
DLPQRVRNKRERYGVYRDRALADLTAWIAEPSLFRQTVAANSASM
CCHHHHHHHHHHCCHHHHHHHHHHHHHHHCHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA