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The map label for this gene is dcm [H]

Identifier: 158335733

GI number: 158335733

Start: 2620068

End: 2621261

Strand: Reverse

Name: dcm [H]

Synonym: AM1_2583

Alternate gene names: 158335733

Gene position: 2621261-2620068 (Counterclockwise)

Preceding gene: 158335734

Following gene: 158335732

Centisome position: 40.3

GC content: 40.7

Gene sequence:

>1194_bases
ATGCCTAAATTATCCTGTTTAGAGTTGTTCACAGGAGCTGGAGGTCTGGCAAAAGGTCTAGAAAAGGCTGGAGTTCAGCA
CACGGCTTTTGTTGAATGGAACAAGGATGCTTGCATCACTTTAGCTAATAACTACAGTGCTCAGCTTGTTCATAATGTAG
ATATTCGTACATTTAAATTTTCACAATTTGGTCATGTTGACATAGTTTCTGGTGGACCTCCATGTCAACCATTTTCGATG
GGAGGAAAACATAAAGGGAATATGGATAAACGAGATATGTTCCCTTACGCCTGTAAAGCGATAAGTGTTTGTACACCTAA
AGCTTTTGTCTTTGAGAATGTCAAAGGGTTGCTACGTAAGTCCTTCAGTAGCTATTTTGAGTACATTCTCTTGAGGTTGA
GATATCCTGACCTTTCTCTTCAAGCTTCAGAACGTTGGGAAGATCATCTTGCAAGGCTTGAGAAGATTCACACTTCAGGG
AAGTATGATGGCATCAAATATAATGTTGTTTTTCGGCTAGTAGACGCTGCAAATTATGGGGTTCCTCAGCGCCGAGAAAG
AGTTTTTATTGTTGGAATTAGAGAAGATTTAAATATCGAGTGGTCTTTTCCTGAAGAATCTCATTCTTTAGATTCTCTAT
TGTGGTCACAATTTATTACTGGCGCTTACTGGATAAGAAATGCGGTCAATCCCTTAGAAATTGAACATCTAGACCAAAGA
ACACAACAACGAGCTAAGCAGCTCATGCAACAGCCTAGTTTATTTTCTCCTTCTCTAGAACCCTGGAAGACAGTTCGGGA
TCAAATTGGAAAACTTCCAGCTCCTGACATCCAAGGTAGTTTTGATAATGAACATGTCTTGAGAGAAGGTGCGCGATCTT
ATCCTGGACATACTGGTAGCTATATTGATATGCCCTCTAAAGCCCTTAAGGCAGGAGATCATGGAGTTCCTGGTGGAGAA
AATATGATTAGATATCAAGATGGTCGAATACGCTATTACACTACGTTTGAAGCTAAAAGAATCCAGACCTTTCCAGACAA
TTATAGGATTTCTGGCTCATGGACAGAAGCAATGAGGCAGATCGGTAATGCTGTACCTGTAGAGTTGGGTTATCGAATAG
CTAACTCTTTAGTCCAAGCTATATCTCCCCGGTCAAATGAAACAGCTAACACTTCGTTGGTGCGGACGGGATAA

Upstream 100 bases:

>100_bases
CCAGGCCGCCTTTGGGCGGAAAAATGTCAGGGAGTTCACGCTAATAAAGCAGATCTCTTGCAAAGCATTGAAGCTTTTAT
GAATGAATTGAATGAAGAAG

Downstream 100 bases:

>100_bases
AGGGATTGATAAAACTTAGCAGTCGCTTGCCGCCGCACAACTCCACCGTTATGGTGCTTCGCATCCAGAGTAGCTTATCT
GAAGAAAAATATCAGGCTGC

Product: DNA-cytosine methyltransferase

Products: NA

Alternate protein names: M.NgoMIV; Cytosine-specific methyltransferase NgoMIV [H]

Number of amino acids: Translated: 397; Mature: 396

Protein sequence:

>397_residues
MPKLSCLELFTGAGGLAKGLEKAGVQHTAFVEWNKDACITLANNYSAQLVHNVDIRTFKFSQFGHVDIVSGGPPCQPFSM
GGKHKGNMDKRDMFPYACKAISVCTPKAFVFENVKGLLRKSFSSYFEYILLRLRYPDLSLQASERWEDHLARLEKIHTSG
KYDGIKYNVVFRLVDAANYGVPQRRERVFIVGIREDLNIEWSFPEESHSLDSLLWSQFITGAYWIRNAVNPLEIEHLDQR
TQQRAKQLMQQPSLFSPSLEPWKTVRDQIGKLPAPDIQGSFDNEHVLREGARSYPGHTGSYIDMPSKALKAGDHGVPGGE
NMIRYQDGRIRYYTTFEAKRIQTFPDNYRISGSWTEAMRQIGNAVPVELGYRIANSLVQAISPRSNETANTSLVRTG

Sequences:

>Translated_397_residues
MPKLSCLELFTGAGGLAKGLEKAGVQHTAFVEWNKDACITLANNYSAQLVHNVDIRTFKFSQFGHVDIVSGGPPCQPFSM
GGKHKGNMDKRDMFPYACKAISVCTPKAFVFENVKGLLRKSFSSYFEYILLRLRYPDLSLQASERWEDHLARLEKIHTSG
KYDGIKYNVVFRLVDAANYGVPQRRERVFIVGIREDLNIEWSFPEESHSLDSLLWSQFITGAYWIRNAVNPLEIEHLDQR
TQQRAKQLMQQPSLFSPSLEPWKTVRDQIGKLPAPDIQGSFDNEHVLREGARSYPGHTGSYIDMPSKALKAGDHGVPGGE
NMIRYQDGRIRYYTTFEAKRIQTFPDNYRISGSWTEAMRQIGNAVPVELGYRIANSLVQAISPRSNETANTSLVRTG
>Mature_396_residues
PKLSCLELFTGAGGLAKGLEKAGVQHTAFVEWNKDACITLANNYSAQLVHNVDIRTFKFSQFGHVDIVSGGPPCQPFSMG
GKHKGNMDKRDMFPYACKAISVCTPKAFVFENVKGLLRKSFSSYFEYILLRLRYPDLSLQASERWEDHLARLEKIHTSGK
YDGIKYNVVFRLVDAANYGVPQRRERVFIVGIREDLNIEWSFPEESHSLDSLLWSQFITGAYWIRNAVNPLEIEHLDQRT
QQRAKQLMQQPSLFSPSLEPWKTVRDQIGKLPAPDIQGSFDNEHVLREGARSYPGHTGSYIDMPSKALKAGDHGVPGGEN
MIRYQDGRIRYYTTFEAKRIQTFPDNYRISGSWTEAMRQIGNAVPVELGYRIANSLVQAISPRSNETANTSLVRTG

Specific function: This methylase recognizes the double-stranded sequence GCCGGC, causes specific methylation on C-2 on both strands, and protects the DNA from cleavage by the ngomIV endonuclease [H]

COG id: COG0270

COG function: function code L; Site-specific DNA methylase

Gene ontology:
GO:0003677: C-5 cytosine-specific DNA methylase
GO:0006306: C-5 cytosine-specific DNA methylase
GO:0008168: C-5 cytosine-specific DNA methylase
GO:0032259: C-5 cytosine-specific DNA methylase
GO:0003886: Cytosine-specific methyltransferase
GO:0009307: Cytosine-specific methyltransferase
GO:0016740: Cytosine-specific methyltransferase
GO:0090116: Cytosine-specific methyltransferase

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the C5-methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1788271, Length=226, Percent_Identity=29.2035398230088, Blast_Score=75, Evalue=6e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018117
- InterPro:   IPR001525 [H]

Pfam domain/function: PF00145 DNA_methylase [H]

EC number: =2.1.1.37 [H]

Molecular weight: Translated: 44918; Mature: 44787

Theoretical pI: Translated: 9.00; Mature: 9.00

Prosite motif: PS00094 C5_MTASE_1 ; PS00095 C5_MTASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPKLSCLELFTGAGGLAKGLEKAGVQHTAFVEWNKDACITLANNYSAQLVHNVDIRTFKF
CCCCHHHHHHHCCCHHHHHHHHCCCCEEEEEEECCCEEEEEECCCCEEEEEECCEEEEEE
SQFGHVDIVSGGPPCQPFSMGGKHKGNMDKRDMFPYACKAISVCTPKAFVFENVKGLLRK
CCCCCEEEECCCCCCCCCCCCCCCCCCCCHHHCCHHHHHHHHHCCCHHHHHHHHHHHHHH
SFSSYFEYILLRLRYPDLSLQASERWEDHLARLEKIHTSGKYDGIKYNVVFRLVDAANYG
HHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEEEEHHHCCC
VPQRRERVFIVGIREDLNIEWSFPEESHSLDSLLWSQFITGAYWIRNAVNPLEIEHLDQR
CCCCCCEEEEEEECCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
TQQRAKQLMQQPSLFSPSLEPWKTVRDQIGKLPAPDIQGSFDNEHVLREGARSYPGHTGS
HHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCC
YIDMPSKALKAGDHGVPGGENMIRYQDGRIRYYTTFEAKRIQTFPDNYRISGSWTEAMRQ
CCCCCHHHHHCCCCCCCCCCCEEEEECCEEEEEEEECHHHHCCCCCCEEECCCHHHHHHH
IGNAVPVELGYRIANSLVQAISPRSNETANTSLVRTG
HCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCEEECC
>Mature Secondary Structure 
PKLSCLELFTGAGGLAKGLEKAGVQHTAFVEWNKDACITLANNYSAQLVHNVDIRTFKF
CCCHHHHHHHCCCHHHHHHHHCCCCEEEEEEECCCEEEEEECCCCEEEEEECCEEEEEE
SQFGHVDIVSGGPPCQPFSMGGKHKGNMDKRDMFPYACKAISVCTPKAFVFENVKGLLRK
CCCCCEEEECCCCCCCCCCCCCCCCCCCCHHHCCHHHHHHHHHCCCHHHHHHHHHHHHHH
SFSSYFEYILLRLRYPDLSLQASERWEDHLARLEKIHTSGKYDGIKYNVVFRLVDAANYG
HHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEEEEHHHCCC
VPQRRERVFIVGIREDLNIEWSFPEESHSLDSLLWSQFITGAYWIRNAVNPLEIEHLDQR
CCCCCCEEEEEEECCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
TQQRAKQLMQQPSLFSPSLEPWKTVRDQIGKLPAPDIQGSFDNEHVLREGARSYPGHTGS
HHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCC
YIDMPSKALKAGDHGVPGGENMIRYQDGRIRYYTTFEAKRIQTFPDNYRISGSWTEAMRQ
CCCCCHHHHHCCCCCCCCCCCEEEEECCEEEEEEEECHHHHCCCCCCEEECCCHHHHHHH
IGNAVPVELGYRIANSLVQAISPRSNETANTSLVRTG
HCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1321116 [H]