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The map label for this gene is spoT [H]

Identifier: 158335664

GI number: 158335664

Start: 2548527

End: 2550788

Strand: Direct

Name: spoT [H]

Synonym: AM1_2514

Alternate gene names: 158335664

Gene position: 2548527-2550788 (Clockwise)

Preceding gene: 158335659

Following gene: 158335665

Centisome position: 39.19

GC content: 51.64

Gene sequence:

>2262_bases
ATGAATCTGAAAGCTACAGTCTCACCGACCGATCTCGAACTGCCCATTTGGCTACAGGAATGTATGCTGGAGGTCGATGG
TGCTGAAGACAAAGATGGCATCCCCGAACAAACTCTAATCTGTCGCGCCTTTAATTTTGGGTATCAACTCCATGAAGGCC
AACGACGGGCCTCTGGAGAACCCTATATTGCCCACCCCGTGTCCGTTGCCGAGCTGCTCCGGGATTTAGGAGGTAGCGCA
GCCATGATCGCCGCTGGCTTCCTCCATGATGTCGTCGAAGATACAGACGTCACCCCCGAAACCATCGAAGCGGAATTTGG
TCTAGAAGTTCGCCAGCTCGTTGAAGGCGTCACCAAGCTTTCTAAATTTAATTTTTCTACCAAAACCGAGCGGGAAGCCG
AAAACTTCCGCCGCATGTTCTTGGCCATGGCCCAAGATATTCGGGTAATTGTGGTCAAACTCGCCGATCGCCTCCACAAT
ATGCGGACCTTAGAGCATCTCCCCCCGGTCAAACAGCGGCGTATTGCCCAGGAAACCCGAGATGTCTTTGCTCCTCTAGC
CAACCGCCTCGGAATTTGGCGGTTCAAGTGGGAATTGGAAGATCTCGCCTTTAAGTATCTAGAACCCGATACCTATCGCC
GCATGCAGCAGCTGGTTGCCGATAAGCGCACGGATCGGGAAGACCATATTGAGCAAGCGATCACCATTCTGCATGATCGC
CTGGAACCCATTTGTTCGAAGCACCTGGAAATTTCTGGCCGCCCTAAACATCTGTTTAGCATTAGCCAAAAAATGGAGCG
GCAACAGAAGGAATTTCATGAAATTTATGATGTCGCCGCCGTTCGGGTGATCGTTAAAACCAACGAAGAATGTTACCGCG
CTTTAGCGGTGGTCCATGACTGTTTCCGTCCCATCCCCGGACGGTTCAAAGACTATATTGGCTTGCCCAAATCCAACCGC
TACCAATCGCTTCATACCGTTGTCATTGGCCTAGGGGGGCGGCCTCTAGAAGTGCAAATTCGTACCGAAGCCATGCACCA
TATCGCCGAGTATGGAATTGCCGCCCACTGGAAATATAAAGAGTCTTCCAACCAGCCAGGGAAAGCCGCTTGGAGCCCGG
ACGACGAAAAATTTACTTGGCTGCGTCAACTGCTTGAGTGGCAAAGCGATCTCAAAGACGCTCAAGAATACTTAGACAAC
GTCAAAGACAATTTATTTGACAGCGAAGTCTATGTGTTTACCCCTAAAGGCGATCTAATCGATCTCACCCAAGGGGCCAC
ACCCGTCGATTTCGCCTACCGCATTCATACAGAAGTCGGTAACCACTGCTTTGGGGCACGGGTCAATGGCCGCATGGTCA
CCCTCGACACGCCCCTGGATAATGGCGACATTGTCGAAATCATGACCCAAAACAGTGCCCATCCCAGTTTGGATTGGCTC
AATTTTGCTGCTAGTAATGCGGCTCGCAACCGGATTCGGCAATGGTATAAACGCTCCCACCGAGAAGAAAATATCTTGCG
CGGCCGCGAGATGCTGGAAAAAGCCCTGGGCAAAAAAGGCTTTGATGCCTTATTGAAATCAGAGCCCATGCAGATTGTTG
CCAATCGATGCAATTACCAAACCCCTGACGATCTCCTAGCTGCCCTCGGCTACGGTGAAGTGACCCTCAATTCCGTCGCC
AACCGGCTGCGAGAAACCATTCGGAGTCAGCAAGCCGAGGCCGAGCCTGCGACCTCAGACCTAACCGACGTTAATTTACC
CACCCAAGCCAGCCCCAGGCCCCTCCCCAGCTCCGGGGACTCTCCCATTACCGGGGTTGAAGGGTTGGTCTACCATATGG
CGGGTTGCTGTCATCCCGTGCCGGGTGAACCGATTATTGGAGCGGTCACCCTCAGTAACCGAGGGATTTCCATTCACCGC
CAAGGCTGTAAGAATATCGGCAATATCCCAGGCGATCGGCTGATTCCCGTCAGTTGGAACCCGACCCACCCTTCCCAACA
AAGTCGCCGCTATACCTACCCCATTGAAGTTCAAATTGAAGTCATCGATCGGGTCGGCATTCTCAAAGATATTTTGACCC
GTTTAACCGACAGTAAAGTCAACGTCCATAATGCCCAAGTTAAAACCTTCCCAGGTCAGACTGCGGTGATCAATCTGGGA
CTAGACATCGAACATTGCAATCAATTAGACCAAATTTGCGCACAAATCCGAAAAATGAGTGATGTTCTCAAACTAAGGCG
TCTCAGCCAGGTTGAAGACTGA

Upstream 100 bases:

>100_bases
ATACAAATAAATTAAGATTGCACTTTTATAAAAGCTAATCCATAGGGGTCAAGCCTCTGTTAAGATTTGTTTAACTGTCT
TCTCCGTACCCCGATGTAGC

Downstream 100 bases:

>100_bases
AATACAGCCATGGACGCTTCCATAATCAATGAGGTGGCGAAATCAGCAGTGCCATGGGTTATGATGGAGCGAAAATCGTT
AATTTTTACTAAACCTTTGC

Product: ppGpp 3'-pyrophosphohydrolase

Products: NA

Alternate protein names: Penta-phosphate guanosine-3'-pyrophosphohydrolase; (ppGpp)ase [H]

Number of amino acids: Translated: 753; Mature: 753

Protein sequence:

>753_residues
MNLKATVSPTDLELPIWLQECMLEVDGAEDKDGIPEQTLICRAFNFGYQLHEGQRRASGEPYIAHPVSVAELLRDLGGSA
AMIAAGFLHDVVEDTDVTPETIEAEFGLEVRQLVEGVTKLSKFNFSTKTEREAENFRRMFLAMAQDIRVIVVKLADRLHN
MRTLEHLPPVKQRRIAQETRDVFAPLANRLGIWRFKWELEDLAFKYLEPDTYRRMQQLVADKRTDREDHIEQAITILHDR
LEPICSKHLEISGRPKHLFSISQKMERQQKEFHEIYDVAAVRVIVKTNEECYRALAVVHDCFRPIPGRFKDYIGLPKSNR
YQSLHTVVIGLGGRPLEVQIRTEAMHHIAEYGIAAHWKYKESSNQPGKAAWSPDDEKFTWLRQLLEWQSDLKDAQEYLDN
VKDNLFDSEVYVFTPKGDLIDLTQGATPVDFAYRIHTEVGNHCFGARVNGRMVTLDTPLDNGDIVEIMTQNSAHPSLDWL
NFAASNAARNRIRQWYKRSHREENILRGREMLEKALGKKGFDALLKSEPMQIVANRCNYQTPDDLLAALGYGEVTLNSVA
NRLRETIRSQQAEAEPATSDLTDVNLPTQASPRPLPSSGDSPITGVEGLVYHMAGCCHPVPGEPIIGAVTLSNRGISIHR
QGCKNIGNIPGDRLIPVSWNPTHPSQQSRRYTYPIEVQIEVIDRVGILKDILTRLTDSKVNVHNAQVKTFPGQTAVINLG
LDIEHCNQLDQICAQIRKMSDVLKLRRLSQVED

Sequences:

>Translated_753_residues
MNLKATVSPTDLELPIWLQECMLEVDGAEDKDGIPEQTLICRAFNFGYQLHEGQRRASGEPYIAHPVSVAELLRDLGGSA
AMIAAGFLHDVVEDTDVTPETIEAEFGLEVRQLVEGVTKLSKFNFSTKTEREAENFRRMFLAMAQDIRVIVVKLADRLHN
MRTLEHLPPVKQRRIAQETRDVFAPLANRLGIWRFKWELEDLAFKYLEPDTYRRMQQLVADKRTDREDHIEQAITILHDR
LEPICSKHLEISGRPKHLFSISQKMERQQKEFHEIYDVAAVRVIVKTNEECYRALAVVHDCFRPIPGRFKDYIGLPKSNR
YQSLHTVVIGLGGRPLEVQIRTEAMHHIAEYGIAAHWKYKESSNQPGKAAWSPDDEKFTWLRQLLEWQSDLKDAQEYLDN
VKDNLFDSEVYVFTPKGDLIDLTQGATPVDFAYRIHTEVGNHCFGARVNGRMVTLDTPLDNGDIVEIMTQNSAHPSLDWL
NFAASNAARNRIRQWYKRSHREENILRGREMLEKALGKKGFDALLKSEPMQIVANRCNYQTPDDLLAALGYGEVTLNSVA
NRLRETIRSQQAEAEPATSDLTDVNLPTQASPRPLPSSGDSPITGVEGLVYHMAGCCHPVPGEPIIGAVTLSNRGISIHR
QGCKNIGNIPGDRLIPVSWNPTHPSQQSRRYTYPIEVQIEVIDRVGILKDILTRLTDSKVNVHNAQVKTFPGQTAVINLG
LDIEHCNQLDQICAQIRKMSDVLKLRRLSQVED
>Mature_753_residues
MNLKATVSPTDLELPIWLQECMLEVDGAEDKDGIPEQTLICRAFNFGYQLHEGQRRASGEPYIAHPVSVAELLRDLGGSA
AMIAAGFLHDVVEDTDVTPETIEAEFGLEVRQLVEGVTKLSKFNFSTKTEREAENFRRMFLAMAQDIRVIVVKLADRLHN
MRTLEHLPPVKQRRIAQETRDVFAPLANRLGIWRFKWELEDLAFKYLEPDTYRRMQQLVADKRTDREDHIEQAITILHDR
LEPICSKHLEISGRPKHLFSISQKMERQQKEFHEIYDVAAVRVIVKTNEECYRALAVVHDCFRPIPGRFKDYIGLPKSNR
YQSLHTVVIGLGGRPLEVQIRTEAMHHIAEYGIAAHWKYKESSNQPGKAAWSPDDEKFTWLRQLLEWQSDLKDAQEYLDN
VKDNLFDSEVYVFTPKGDLIDLTQGATPVDFAYRIHTEVGNHCFGARVNGRMVTLDTPLDNGDIVEIMTQNSAHPSLDWL
NFAASNAARNRIRQWYKRSHREENILRGREMLEKALGKKGFDALLKSEPMQIVANRCNYQTPDDLLAALGYGEVTLNSVA
NRLRETIRSQQAEAEPATSDLTDVNLPTQASPRPLPSSGDSPITGVEGLVYHMAGCCHPVPGEPIIGAVTLSNRGISIHR
QGCKNIGNIPGDRLIPVSWNPTHPSQQSRRYTYPIEVQIEVIDRVGILKDILTRLTDSKVNVHNAQVKTFPGQTAVINLG
LDIEHCNQLDQICAQIRKMSDVLKLRRLSQVED

Specific function: In eubacteria ppGpp (guanosine 3'-diphosphate 5-' diphosphate) is a mediator of the stringent response that coordinates a variety of cellular activities in response to changes in nutritional abundance. This enzyme catalyzes the degradation of ppGpp into G

COG id: COG0317

COG function: function code TK; Guanosine polyphosphate pyrophosphohydrolases/synthetases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HD domain [H]

Homologues:

Organism=Homo sapiens, GI38348360, Length=140, Percent_Identity=37.1428571428571, Blast_Score=73, Evalue=1e-12,
Organism=Escherichia coli, GI1790082, Length=708, Percent_Identity=39.8305084745763, Blast_Score=518, Evalue=1e-148,
Organism=Escherichia coli, GI1789147, Length=731, Percent_Identity=35.8413132694938, Blast_Score=485, Evalue=1e-138,
Organism=Caenorhabditis elegans, GI25143118, Length=136, Percent_Identity=34.5588235294118, Blast_Score=78, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002912
- InterPro:   IPR012675
- InterPro:   IPR003607
- InterPro:   IPR007685
- InterPro:   IPR004811
- InterPro:   IPR004095
- InterPro:   IPR012676 [H]

Pfam domain/function: PF01842 ACT; PF04607 RelA_SpoT; PF02824 TGS [H]

EC number: =3.1.7.2 [H]

Molecular weight: Translated: 85458; Mature: 85458

Theoretical pI: Translated: 6.48; Mature: 6.48

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLKATVSPTDLELPIWLQECMLEVDGAEDKDGIPEQTLICRAFNFGYQLHEGQRRASGE
CCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCHHCCCC
PYIAHPVSVAELLRDLGGSAAMIAAGFLHDVVEDTDVTPETIEAEFGLEVRQLVEGVTKL
CEEECHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHCCHHHHHHHHHHHHH
SKFNFSTKTEREAENFRRMFLAMAQDIRVIVVKLADRLHNMRTLEHLPPVKQRRIAQETR
HHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
DVFAPLANRLGIWRFKWELEDLAFKYLEPDTYRRMQQLVADKRTDREDHIEQAITILHDR
HHHHHHHHHCCEEEEEEEHHHHHHHHCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
LEPICSKHLEISGRPKHLFSISQKMERQQKEFHEIYDVAAVRVIVKTNEECYRALAVVHD
HHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCHHHHHHHHHHHH
CFRPIPGRFKDYIGLPKSNRYQSLHTVVIGLGGRPLEVQIRTEAMHHIAEYGIAAHWKYK
HHCCCCCHHHHHCCCCCCCCHHHEEEEEEECCCCEEEEEEHHHHHHHHHHCCCCEEEEEC
ESSNQPGKAAWSPDDEKFTWLRQLLEWQSDLKDAQEYLDNVKDNLFDSEVYVFTPKGDLI
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCEE
DLTQGATPVDFAYRIHTEVGNHCFGARVNGRMVTLDTPLDNGDIVEIMTQNSAHPSLDWL
ECCCCCCCHHHHEEEHHHHCCCEECCEECCEEEEEECCCCCCCEEEEEECCCCCCCHHHH
NFAASNAARNRIRQWYKRSHREENILRGREMLEKALGKKGFDALLKSEPMQIVANRCNYQ
HHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCHHHHHHCCCHHHHHHHCCCC
TPDDLLAALGYGEVTLNSVANRLRETIRSQQAEAEPATSDLTDVNLPTQASPRPLPSSGD
CHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
SPITGVEGLVYHMAGCCHPVPGEPIIGAVTLSNRGISIHRQGCKNIGNIPGDRLIPVSWN
CCCCHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCCEEEHHHHHHCCCCCCCCEEEECCC
PTHPSQQSRRYTYPIEVQIEVIDRVGILKDILTRLTDSKVNVHNAQVKTFPGQTAVINLG
CCCCCHHCCCCCCCEEEEEEHHHHHHHHHHHHHHHCCCCCEEECCEEEECCCCEEEEEEC
LDIEHCNQLDQICAQIRKMSDVLKLRRLSQVED
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNLKATVSPTDLELPIWLQECMLEVDGAEDKDGIPEQTLICRAFNFGYQLHEGQRRASGE
CCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCHHCCCC
PYIAHPVSVAELLRDLGGSAAMIAAGFLHDVVEDTDVTPETIEAEFGLEVRQLVEGVTKL
CEEECHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHCCHHHHHHHHHHHHH
SKFNFSTKTEREAENFRRMFLAMAQDIRVIVVKLADRLHNMRTLEHLPPVKQRRIAQETR
HHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
DVFAPLANRLGIWRFKWELEDLAFKYLEPDTYRRMQQLVADKRTDREDHIEQAITILHDR
HHHHHHHHHCCEEEEEEEHHHHHHHHCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
LEPICSKHLEISGRPKHLFSISQKMERQQKEFHEIYDVAAVRVIVKTNEECYRALAVVHD
HHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCHHHHHHHHHHHH
CFRPIPGRFKDYIGLPKSNRYQSLHTVVIGLGGRPLEVQIRTEAMHHIAEYGIAAHWKYK
HHCCCCCHHHHHCCCCCCCCHHHEEEEEEECCCCEEEEEEHHHHHHHHHHCCCCEEEEEC
ESSNQPGKAAWSPDDEKFTWLRQLLEWQSDLKDAQEYLDNVKDNLFDSEVYVFTPKGDLI
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCEE
DLTQGATPVDFAYRIHTEVGNHCFGARVNGRMVTLDTPLDNGDIVEIMTQNSAHPSLDWL
ECCCCCCCHHHHEEEHHHHCCCEECCEECCEEEEEECCCCCCCEEEEEECCCCCCCHHHH
NFAASNAARNRIRQWYKRSHREENILRGREMLEKALGKKGFDALLKSEPMQIVANRCNYQ
HHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCHHHHHHCCCHHHHHHHCCCC
TPDDLLAALGYGEVTLNSVANRLRETIRSQQAEAEPATSDLTDVNLPTQASPRPLPSSGD
CHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
SPITGVEGLVYHMAGCCHPVPGEPIIGAVTLSNRGISIHRQGCKNIGNIPGDRLIPVSWN
CCCCHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCCEEEHHHHHHCCCCCCCCEEEECCC
PTHPSQQSRRYTYPIEVQIEVIDRVGILKDILTRLTDSKVNVHNAQVKTFPGQTAVINLG
CCCCCHHCCCCCCCEEEEEEHHHHHHHHHHHHHHHCCCCCEEECCEEEECCCCEEEEEEC
LDIEHCNQLDQICAQIRKMSDVLKLRRLSQVED
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]