Click here to switch to the map view.

The map label for this gene is ndhM

Identifier: 158335647

GI number: 158335647

Start: 2521910

End: 2523514

Strand: Direct

Name: ndhM

Synonym: AM1_2497

Alternate gene names: 158335647

Gene position: 2521910-2523514 (Clockwise)

Preceding gene: 158335646

Following gene: 158335648

Centisome position: 38.78

GC content: 49.6

Gene sequence:

>1605_bases
ATGAATCCAGATACATTTCCTTGGCTGTCAACCCTGATTTTCTTCCCAATTATGGCCACCGTCGCCCTGCCCTTTATTCC
AGATCCAAAGGGTAAAGGTGATCCCATTCGCTGGTATGCACTGGTGATTGGCCTGATTGATTTTGTGTTATTGATCTACG
CCTTTTACACCCAGTACGACTTTGCCGAGTCTGGGCTGCAAATGGTGGAACACTACGACTGGCTGCCCCAACTAGGGGTG
CAATGGTCTGTTGGGGCAGACGGCTTATCCATGCCCCTGATTTTGCTTACGGGATTTATTACTTCTTTAGCGATCCTGGC
CTCCTGGCCCGTTACCTATAAGCCCAGACTTTTCTATTTCTTGATTTTGGCCATGTACGGTGGTCAGATTGCGGTGTTCG
CTGTTCAAGATCTGCTGGTCTTTTTCTTGGTCTGGGAATTGGAATTAGTGCCAGTGTATCTGTTGCTCTCCATTTGGGGC
GGCTACAAACGGCTTTACGCAGCGACGAAATTTATTCTCTATACCGCCATCAGCTCCCTATTTATTCTGGTAGCAGCTTT
GGCAATGGCCTTCTTTGGTCCCGACCTCACCTTTGACCTGCAGTCCCTAGCGGCAAAAGATTATCCGTTAACGTTCCAAC
TGCTCTGTTACACGGGCTTTTTGGTTGCCTTTGCAGTCAAGTTGCCAATTGTGCCCCTACATACCTGGTTACCGGATGCC
CACGGCGAAGCCACAGCTCCAGTCCATATGCTGTTGGCCGGAATCCTCCTAAAGATGGGAGGCTATGCGTTAATTCGCAT
GAATGTGGAGTTACTCCCTGATGCCCATGCCTATTTTGCTCCCGCTCTGATTATTCTGGGTGTCGTCAATATTATTTACG
CGGCCCTCACCTCGTTTGCCCAGCGCAATCTCAAACGAAAAATCGCCTATTCATCCATTTCCCATATGGGCTTTGTCCTG
ATCGGCATTGCCTCCTTTACGGATTTGGGAATGAGTGGTGCCGTTCTGCAAATGGTCTCTCACGGCTTGATTGGTGCTAG
TCTCTTCTTTTTAGTGGGAGCGACTTACGATCGCACTCACACTTTGATCCTGGAAGAGATGGGAGGCGTGGCCCAGAATA
TGCCCAAAATCTTTGCCATGTTCACCACCTGTTCCATGGCGTCTTTAGCCCTACCAGGGATGAGCGGTTTTGTCGCGGAA
CTGATGGTGTTTGTTGGACTAGCCACCAGCGATGCCTATAGCCTCGCTTTCCGCGTACCGGTGGTGATTTTGGCAGGCAT
TGGTGTCATTCTGACGCCAATTTATCTGCTGTCCATGCTGCGGGAAATCTTCTATGGCCCTGAGAACAAAGAACTCACGT
CCCACGAGAAGCTAGTCGATGCCGAACCTCGGGAAATCTTTGTGATTGCCTGCTTACTCGTGCCCATTATTGGTATTGGC
CTCTATCCCAAAATCATTACCCAGATTTACGATGCCAAGACCACCCAGTTAGTGGCTTATATTCGCCCCTCTGTTCCTTC
TATAGCCATGCGAGAGGCGGCTACAGTGGCATCTGTTTCCGATATTGAGATGGACAGCTCTTATCAAGCTCCCGCGATTA
AGTAA

Upstream 100 bases:

>100_bases
TCTTTATGGCAGTCTTGGGGATCTCGATTGCCACGGTCATCTAAAGTCTTTTCATTTTTTCCTCGTCGTTGCTCAATCCG
TTGAATAGAGATAGCGGTTC

Downstream 100 bases:

>100_bases
GTCGCTCACTATTTCACACCTAAAATTTATTTAATAGGTATACAAACCCCCATGCTGAAATTCAGTGTGGGGGTTTCTGA
CATTTGACATCGATTGAGAC

Product: NAD(P)H-quinone oxidoreductase subunit 4

Products: NAD+; ubiquinol

Alternate protein names: NAD(P)H dehydrogenase I, chain 4; NDH-1, chain 4

Number of amino acids: Translated: 534; Mature: 534

Protein sequence:

>534_residues
MNPDTFPWLSTLIFFPIMATVALPFIPDPKGKGDPIRWYALVIGLIDFVLLIYAFYTQYDFAESGLQMVEHYDWLPQLGV
QWSVGADGLSMPLILLTGFITSLAILASWPVTYKPRLFYFLILAMYGGQIAVFAVQDLLVFFLVWELELVPVYLLLSIWG
GYKRLYAATKFILYTAISSLFILVAALAMAFFGPDLTFDLQSLAAKDYPLTFQLLCYTGFLVAFAVKLPIVPLHTWLPDA
HGEATAPVHMLLAGILLKMGGYALIRMNVELLPDAHAYFAPALIILGVVNIIYAALTSFAQRNLKRKIAYSSISHMGFVL
IGIASFTDLGMSGAVLQMVSHGLIGASLFFLVGATYDRTHTLILEEMGGVAQNMPKIFAMFTTCSMASLALPGMSGFVAE
LMVFVGLATSDAYSLAFRVPVVILAGIGVILTPIYLLSMLREIFYGPENKELTSHEKLVDAEPREIFVIACLLVPIIGIG
LYPKIITQIYDAKTTQLVAYIRPSVPSIAMREAATVASVSDIEMDSSYQAPAIK

Sequences:

>Translated_534_residues
MNPDTFPWLSTLIFFPIMATVALPFIPDPKGKGDPIRWYALVIGLIDFVLLIYAFYTQYDFAESGLQMVEHYDWLPQLGV
QWSVGADGLSMPLILLTGFITSLAILASWPVTYKPRLFYFLILAMYGGQIAVFAVQDLLVFFLVWELELVPVYLLLSIWG
GYKRLYAATKFILYTAISSLFILVAALAMAFFGPDLTFDLQSLAAKDYPLTFQLLCYTGFLVAFAVKLPIVPLHTWLPDA
HGEATAPVHMLLAGILLKMGGYALIRMNVELLPDAHAYFAPALIILGVVNIIYAALTSFAQRNLKRKIAYSSISHMGFVL
IGIASFTDLGMSGAVLQMVSHGLIGASLFFLVGATYDRTHTLILEEMGGVAQNMPKIFAMFTTCSMASLALPGMSGFVAE
LMVFVGLATSDAYSLAFRVPVVILAGIGVILTPIYLLSMLREIFYGPENKELTSHEKLVDAEPREIFVIACLLVPIIGIG
LYPKIITQIYDAKTTQLVAYIRPSVPSIAMREAATVASVSDIEMDSSYQAPAIK
>Mature_534_residues
MNPDTFPWLSTLIFFPIMATVALPFIPDPKGKGDPIRWYALVIGLIDFVLLIYAFYTQYDFAESGLQMVEHYDWLPQLGV
QWSVGADGLSMPLILLTGFITSLAILASWPVTYKPRLFYFLILAMYGGQIAVFAVQDLLVFFLVWELELVPVYLLLSIWG
GYKRLYAATKFILYTAISSLFILVAALAMAFFGPDLTFDLQSLAAKDYPLTFQLLCYTGFLVAFAVKLPIVPLHTWLPDA
HGEATAPVHMLLAGILLKMGGYALIRMNVELLPDAHAYFAPALIILGVVNIIYAALTSFAQRNLKRKIAYSSISHMGFVL
IGIASFTDLGMSGAVLQMVSHGLIGASLFFLVGATYDRTHTLILEEMGGVAQNMPKIFAMFTTCSMASLALPGMSGFVAE
LMVFVGLATSDAYSLAFRVPVVILAGIGVILTPIYLLSMLREIFYGPENKELTSHEKLVDAEPREIFVIACLLVPIIGIG
LYPKIITQIYDAKTTQLVAYIRPSVPSIAMREAATVASVSDIEMDSSYQAPAIK

Specific function: NDH-1 shuttles electrons from NAD(P)H, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be plastoquinone. Couples the redox reaction to proton tran

COG id: COG1008

COG function: function code C; NADH:ubiquinone oxidoreductase subunit 4 (chain M)

Gene ontology:

Cell location: Cellular thylakoid membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 4 family

Homologues:

Organism=Homo sapiens, GI251831116, Length=385, Percent_Identity=31.4285714285714, Blast_Score=154, Evalue=2e-37,
Organism=Homo sapiens, GI251831117, Length=366, Percent_Identity=25.9562841530055, Blast_Score=66, Evalue=6e-11,
Organism=Escherichia coli, GI1788613, Length=488, Percent_Identity=37.7049180327869, Blast_Score=288, Evalue=6e-79,
Organism=Escherichia coli, GI1788827, Length=284, Percent_Identity=26.056338028169, Blast_Score=97, Evalue=3e-21,
Organism=Escherichia coli, GI1788831, Length=290, Percent_Identity=29.3103448275862, Blast_Score=96, Evalue=8e-21,
Organism=Escherichia coli, GI2367154, Length=207, Percent_Identity=28.0193236714976, Blast_Score=76, Evalue=4e-15,
Organism=Escherichia coli, GI1788829, Length=302, Percent_Identity=27.8145695364238, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI145693160, Length=321, Percent_Identity=30.2180685358255, Blast_Score=74, Evalue=3e-14,
Organism=Escherichia coli, GI1788614, Length=499, Percent_Identity=24.0480961923848, Blast_Score=71, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NU4C_ACAM1 (B0C4B4)

Other databases:

- EMBL:   CP000828
- RefSeq:   YP_001516819.1
- GeneID:   5681309
- GenomeReviews:   CP000828_GR
- KEGG:   amr:AM1_2497
- HOGENOM:   HBG643593
- OMA:   TSFAQRN
- ProtClustDB:   PRK02546
- BioCyc:   AMAR329726:AM1_2497-MONOMER
- GO:   GO:0005739
- HAMAP:   MF_00491
- InterPro:   IPR022997
- InterPro:   IPR010227
- InterPro:   IPR001750
- InterPro:   IPR003918
- InterPro:   IPR000260
- PRINTS:   PR01437
- TIGRFAMs:   TIGR01972

Pfam domain/function: PF00361 Oxidored_q1; PF01059 Oxidored_q5_N

EC number: 1.6.5.3

Molecular weight: Translated: 58669; Mature: 58669

Theoretical pI: Translated: 5.58; Mature: 5.58

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0xe45fc1c)-; HASH(0xf3e7ea8)-; HASH(0xfefa778)-; HASH(0x1036c500)-; HASH(0xede0800)-; HASH(0x10488620)-; HASH(0x1016f330)-; HASH(0xf424e84)-; HASH(0xfdf8a8c)-; HASH(0xf790fdc)-; HASH(0x10485d20)-; HASH(0x10483820)-; HASH(0xf2cde30)-; HASH(0xfade574)-;

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHCCHHHHHHEEECCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCH
>Mature Secondary Structure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HHHHHHHHHHCCHHHHHHEEECCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADH; H+; ubiquinone

Specific reaction: NADH + H+ + ubiquinone = NAD+ + ubiquinol

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA