Click here to switch to the map view.

The map label for this gene is tapB [H]

Identifier: 158335617

GI number: 158335617

Start: 2489474

End: 2491501

Strand: Direct

Name: tapB [H]

Synonym: AM1_2467

Alternate gene names: 158335617

Gene position: 2489474-2491501 (Clockwise)

Preceding gene: 158335614

Following gene: 158335618

Centisome position: 38.28

GC content: 49.26

Gene sequence:

>2028_bases
ATGGAGAATAACTCATCCAAGCGGGAACTGCTCGTTAGAAACAATTTTTCTCCTTCCAATGAAAAATTAATTAAGAGTGG
CTATGTAGATCAAGCACGCTTGCGAGAAGCGATTTATGAAAGTCGGCGGAAGGGACGTTCGCTGCTAGCGATCCTGCAAA
AGATGACGGGGAAACCTTTACCCCCCCGACTTCTTCGACAAATCAAGCGTCAGCAGCTATTTGAGTTAAAAATTCTCTAT
GGACTGGATTGTCTCGATCCTGAGCTGACCCAGGTTGACTTTGACGAAATTGACCGCTTGATTCGCACCGTCATTCCCCT
TGACTATTGTCGTCGCTACCAGATTTTGCCCCTCGCCCTCAATGCCGACGTCGATCCACCAGTTGTCCGTGTGGGCATGG
TCAACCCGGACCACCTCAGTGCCATTGATGATGTTGACTATTTGCTGCGCTCCAATGGGATGACCTTCCAGCGGGTGGTT
CTAGATTTGGATACTTTCCAAATGCTGATCGCCAAGTTCTTGGACGCCCAAGCCAAACGTCAGGCCACCATCCAAGCAAA
CCTGCCCGTTGAATATGGCCTAGAAGATGATCTAGATCGCCTACAGGGGATTGAAGATCTCTCCAGCAATGATGCAGATA
TCAATCTGGATGAAGCCCTACTAGATGAAAATGCAGCGCCAGTCATTTCTTTAGTTAATAAAATATTGGCAAAAGCATTA
CAAAATGGCATTTCTGATATTCATATTGAGCCGCAGGAAGACAATTTGCGCGTTCGTTTTCGCAAAGATGGCTTATTGCA
TAATGCGTTTCAGCCCCTACCTCGGCGCATTGCCCCTGCCCTCGTCTCCCGTTTCAAAATCATCGCCAACTTAGATATCG
CCGAACGGCGTTTACCCCAAGATGGTCGGATTCTCCGGTTATTTCAGGGCCGCAAAGTTGACTTTCGTGTCAGTACCTTA
CCGAGTCGCTATGGCGAAAAGGTCGTTTTGCGGATCTTAGACAACTCATCGATGCAGCTAGGTCTCAACCATCTGATTGA
TGATCCGACCAGCCTCGCAATGGTCCGTGAAATGGTGGATCGGCCTTTTGGGTTGATTTTGGTGACCGGCCCTACTGGTT
CGGGTAAAACGACGACCCTATATTCAGCCTTGACGGAGCGCAATACTCCCGAAGTCAATATCAGTACCGTTGAGGACCCC
ATTGAATATACGCTGCCGGGGATTACCCAGGTACAGGTCTTGCGGGAAAAAGGCATGGACTTTGCCTCCATTCTGCGGGC
CTTTCTACGGCAAGACCCTGATGTCCTACTCGTGGGTGAGACCCGAGACAAGGAAACGGCTAAAACGGCGATTGAAGCCG
CGTTAACGGGACACTTAGTGTTAACAACCCTACACACCAACGATGCAGCCAGTGCCATTGTTCGTTTGGCAGAAATGGAT
GTTGAACCGTTTATGGTCTCAGCGTCCTTATTGGGTGTGGTGGCTCAACGACTCGTCCGCCGCCTCTGTCCAGACTGTCG
AATTCCCTATCGTCCTTCCCCAGAAAAGCTAGCTCAGTTTGGGTTAATGGCTTCGGCCAAAACAGAAATCACCTTTTACA
AGGCCAATACCTCTACGTCTGACGATATTTTGGCAACGGCAAATACAGAGGATGCCTGTCCCACCTGTTCCGGTATCGGC
TATTCCGGGCGATGCGGGGTCTATGAAGTTATGCAGGTGACCGAAAAGTTAAAGGGACTAATTACCGAAGGTGCACCAAC
AGAGCATATCAAGGAAGTGGCTGTTGAAGAAGGGATGCAAACCTTGTTGGCTTATAGTTTGAAGCTGGTTGAGCAAGGCG
CTACATCCTTAGAAGAAGTAGAACGGGTGACCTTCTCTGATAGCGGCTTGAAAACGGAGCTCAAGGCAAAGCGAAAAACA
GCCCTCAACTGTCATGTTTGCAATGCAGGACTCTTACCGGAGTGGTTAGACTGTCCCTACTGCCTAACGCCCAGGTTTGC
AGAGCATCATCCAGCCAATCAACTCTAA

Upstream 100 bases:

>100_bases
GTCTGCATTAGCCTTGGATTTTCCCCATAACCCAAAGTATTTGGGAATACACAGTTTTTTTATCTGTACATTTAACTAAC
TTTATTTCTTTTTTATAGTC

Downstream 100 bases:

>100_bases
AGGGTGTTGTCCCAAAAGAGATCGGGCTATGGCAAGCTGTGAACTGTGAGCTTGCCCCGTTTGCTATTCCTGACTGAACG
TTTTCCACCCGATATGGGCG

Product: general secretion pathway protein E

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 675; Mature: 675

Protein sequence:

>675_residues
MENNSSKRELLVRNNFSPSNEKLIKSGYVDQARLREAIYESRRKGRSLLAILQKMTGKPLPPRLLRQIKRQQLFELKILY
GLDCLDPELTQVDFDEIDRLIRTVIPLDYCRRYQILPLALNADVDPPVVRVGMVNPDHLSAIDDVDYLLRSNGMTFQRVV
LDLDTFQMLIAKFLDAQAKRQATIQANLPVEYGLEDDLDRLQGIEDLSSNDADINLDEALLDENAAPVISLVNKILAKAL
QNGISDIHIEPQEDNLRVRFRKDGLLHNAFQPLPRRIAPALVSRFKIIANLDIAERRLPQDGRILRLFQGRKVDFRVSTL
PSRYGEKVVLRILDNSSMQLGLNHLIDDPTSLAMVREMVDRPFGLILVTGPTGSGKTTTLYSALTERNTPEVNISTVEDP
IEYTLPGITQVQVLREKGMDFASILRAFLRQDPDVLLVGETRDKETAKTAIEAALTGHLVLTTLHTNDAASAIVRLAEMD
VEPFMVSASLLGVVAQRLVRRLCPDCRIPYRPSPEKLAQFGLMASAKTEITFYKANTSTSDDILATANTEDACPTCSGIG
YSGRCGVYEVMQVTEKLKGLITEGAPTEHIKEVAVEEGMQTLLAYSLKLVEQGATSLEEVERVTFSDSGLKTELKAKRKT
ALNCHVCNAGLLPEWLDCPYCLTPRFAEHHPANQL

Sequences:

>Translated_675_residues
MENNSSKRELLVRNNFSPSNEKLIKSGYVDQARLREAIYESRRKGRSLLAILQKMTGKPLPPRLLRQIKRQQLFELKILY
GLDCLDPELTQVDFDEIDRLIRTVIPLDYCRRYQILPLALNADVDPPVVRVGMVNPDHLSAIDDVDYLLRSNGMTFQRVV
LDLDTFQMLIAKFLDAQAKRQATIQANLPVEYGLEDDLDRLQGIEDLSSNDADINLDEALLDENAAPVISLVNKILAKAL
QNGISDIHIEPQEDNLRVRFRKDGLLHNAFQPLPRRIAPALVSRFKIIANLDIAERRLPQDGRILRLFQGRKVDFRVSTL
PSRYGEKVVLRILDNSSMQLGLNHLIDDPTSLAMVREMVDRPFGLILVTGPTGSGKTTTLYSALTERNTPEVNISTVEDP
IEYTLPGITQVQVLREKGMDFASILRAFLRQDPDVLLVGETRDKETAKTAIEAALTGHLVLTTLHTNDAASAIVRLAEMD
VEPFMVSASLLGVVAQRLVRRLCPDCRIPYRPSPEKLAQFGLMASAKTEITFYKANTSTSDDILATANTEDACPTCSGIG
YSGRCGVYEVMQVTEKLKGLITEGAPTEHIKEVAVEEGMQTLLAYSLKLVEQGATSLEEVERVTFSDSGLKTELKAKRKT
ALNCHVCNAGLLPEWLDCPYCLTPRFAEHHPANQL
>Mature_675_residues
MENNSSKRELLVRNNFSPSNEKLIKSGYVDQARLREAIYESRRKGRSLLAILQKMTGKPLPPRLLRQIKRQQLFELKILY
GLDCLDPELTQVDFDEIDRLIRTVIPLDYCRRYQILPLALNADVDPPVVRVGMVNPDHLSAIDDVDYLLRSNGMTFQRVV
LDLDTFQMLIAKFLDAQAKRQATIQANLPVEYGLEDDLDRLQGIEDLSSNDADINLDEALLDENAAPVISLVNKILAKAL
QNGISDIHIEPQEDNLRVRFRKDGLLHNAFQPLPRRIAPALVSRFKIIANLDIAERRLPQDGRILRLFQGRKVDFRVSTL
PSRYGEKVVLRILDNSSMQLGLNHLIDDPTSLAMVREMVDRPFGLILVTGPTGSGKTTTLYSALTERNTPEVNISTVEDP
IEYTLPGITQVQVLREKGMDFASILRAFLRQDPDVLLVGETRDKETAKTAIEAALTGHLVLTTLHTNDAASAIVRLAEMD
VEPFMVSASLLGVVAQRLVRRLCPDCRIPYRPSPEKLAQFGLMASAKTEITFYKANTSTSDDILATANTEDACPTCSGIG
YSGRCGVYEVMQVTEKLKGLITEGAPTEHIKEVAVEEGMQTLLAYSLKLVEQGATSLEEVERVTFSDSGLKTELKAKRKT
ALNCHVCNAGLLPEWLDCPYCLTPRFAEHHPANQL

Specific function: Involved in the translocation of the type IV pilin [H]

COG id: COG2804

COG function: function code NU; Type II secretory pathway, ATPase PulE/Tfp pilus assembly pathway, ATPase PilB

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI1789723, Length=450, Percent_Identity=46.4444444444444, Blast_Score=362, Evalue=1e-101,
Organism=Escherichia coli, GI1786296, Length=409, Percent_Identity=43.0317848410758, Blast_Score=310, Evalue=3e-85,
Organism=Escherichia coli, GI87082188, Length=194, Percent_Identity=43.298969072165, Blast_Score=118, Evalue=1e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013374
- InterPro:   IPR007831
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E; PF05157 GSPII_E_N [H]

EC number: NA

Molecular weight: Translated: 75422; Mature: 75422

Theoretical pI: Translated: 5.62; Mature: 5.62

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MENNSSKRELLVRNNFSPSNEKLIKSGYVDQARLREAIYESRRKGRSLLAILQKMTGKPL
CCCCCCHHEEEEECCCCCCHHHHHHCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHCCCCC
PPRLLRQIKRQQLFELKILYGLDCLDPELTQVDFDEIDRLIRTVIPLDYCRRYQILPLAL
CHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCHHHHCCCEEEEEEE
NADVDPPVVRVGMVNPDHLSAIDDVDYLLRSNGMTFQRVVLDLDTFQMLIAKFLDAQAKR
CCCCCCCEEEEECCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHC
QATIQANLPVEYGLEDDLDRLQGIEDLSSNDADINLDEALLDENAAPVISLVNKILAKAL
CEEEEECCCEECCCCHHHHHHCCHHHCCCCCCCCCHHHHHHCCCCCHHHHHHHHHHHHHH
QNGISDIHIEPQEDNLRVRFRKDGLLHNAFQPLPRRIAPALVSRFKIIANLDIAERRLPQ
HCCCCCEEECCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCC
DGRILRLFQGRKVDFRVSTLPSRYGEKVVLRILDNSSMQLGLNHLIDDPTSLAMVREMVD
CCCEEEEECCCCEEEEEECCCHHHCCEEEEHEECCCCHHHCHHHHCCCHHHHHHHHHHHC
RPFGLILVTGPTGSGKTTTLYSALTERNTPEVNISTVEDPIEYTLPGITQVQVLREKGMD
CCCEEEEEECCCCCCCHHHHHHHHHCCCCCCEEEEECCCCHHHCCCCHHHHHHHHHHCCC
FASILRAFLRQDPDVLLVGETRDKETAKTAIEAALTGHLVLTTLHTNDAASAIVRLAEMD
HHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHCCEEEEEEEECCHHHHHHHHHHHCC
VEPFMVSASLLGVVAQRLVRRLCPDCRIPYRPSPEKLAQFGLMASAKTEITFYKANTSTS
CCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCCEEEEEECCCCCC
DDILATANTEDACPTCSGIGYSGRCGVYEVMQVTEKLKGLITEGAPTEHIKEVAVEEGMQ
CCEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
TLLAYSLKLVEQGATSLEEVERVTFSDSGLKTELKAKRKTALNCHVCNAGLLPEWLDCPY
HHHHHHHHHHHHCCHHHHHHHHHHCCCCCCHHHHHHHHHHCCEEEEECCCCCHHHHCCCC
CLTPRFAEHHPANQL
CCCCCCCCCCCCCCC
>Mature Secondary Structure
MENNSSKRELLVRNNFSPSNEKLIKSGYVDQARLREAIYESRRKGRSLLAILQKMTGKPL
CCCCCCHHEEEEECCCCCCHHHHHHCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHCCCCC
PPRLLRQIKRQQLFELKILYGLDCLDPELTQVDFDEIDRLIRTVIPLDYCRRYQILPLAL
CHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCHHHHCCCEEEEEEE
NADVDPPVVRVGMVNPDHLSAIDDVDYLLRSNGMTFQRVVLDLDTFQMLIAKFLDAQAKR
CCCCCCCEEEEECCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHC
QATIQANLPVEYGLEDDLDRLQGIEDLSSNDADINLDEALLDENAAPVISLVNKILAKAL
CEEEEECCCEECCCCHHHHHHCCHHHCCCCCCCCCHHHHHHCCCCCHHHHHHHHHHHHHH
QNGISDIHIEPQEDNLRVRFRKDGLLHNAFQPLPRRIAPALVSRFKIIANLDIAERRLPQ
HCCCCCEEECCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCC
DGRILRLFQGRKVDFRVSTLPSRYGEKVVLRILDNSSMQLGLNHLIDDPTSLAMVREMVD
CCCEEEEECCCCEEEEEECCCHHHCCEEEEHEECCCCHHHCHHHHCCCHHHHHHHHHHHC
RPFGLILVTGPTGSGKTTTLYSALTERNTPEVNISTVEDPIEYTLPGITQVQVLREKGMD
CCCEEEEEECCCCCCCHHHHHHHHHCCCCCCEEEEECCCCHHHCCCCHHHHHHHHHHCCC
FASILRAFLRQDPDVLLVGETRDKETAKTAIEAALTGHLVLTTLHTNDAASAIVRLAEMD
HHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHCCEEEEEEEECCHHHHHHHHHHHCC
VEPFMVSASLLGVVAQRLVRRLCPDCRIPYRPSPEKLAQFGLMASAKTEITFYKANTSTS
CCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCCEEEEEECCCCCC
DDILATANTEDACPTCSGIGYSGRCGVYEVMQVTEKLKGLITEGAPTEHIKEVAVEEGMQ
CCEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
TLLAYSLKLVEQGATSLEEVERVTFSDSGLKTELKAKRKTALNCHVCNAGLLPEWLDCPY
HHHHHHHHHHHHCCHHHHHHHHHHCCCCCCHHHHHHHHHHCCEEEEECCCCCHHHHCCCC
CLTPRFAEHHPANQL
CCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8820654 [H]