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The map label for this gene is amyS [H]

Identifier: 158335445

GI number: 158335445

Start: 2289974

End: 2291455

Strand: Reverse

Name: amyS [H]

Synonym: AM1_2293

Alternate gene names: 158335445

Gene position: 2291455-2289974 (Counterclockwise)

Preceding gene: 158335446

Following gene: 158335442

Centisome position: 35.23

GC content: 49.19

Gene sequence:

>1482_bases
ATGTCTGAGCCCAACGGCGTAATGATGCAGTTCTTTCATTGGTACAACGCCGCTGATGGCAGTTTGTGGAGTGAACTGAA
GACAAAAGCTGCCGATCTAGCGAATGTGGGTATCACCGCTGTGTGGCTACCGCCTGCCTATAAGGGGCTAGCAGGAGGAT
ATGACGTGGGCTATGGTGTTTATGACATGTATGACTTGGGTGAATTCGACCAAAAAGGGTCTATTCGCACCAAATATGGC
ACTAAAGATGAATATATATCGGCGATTAAGGTCGCTCAAACAGTTGGTATTCATGTCTATGCTGATGTAGTGCTGAATCA
CAAGATGGGCGCGGACGCCGAAGAAGAAGCCCAGGCTCGCCCTATGAATCCTGATAATCGTCACCAGCCCATTGGTGACA
CTCAAACCGTCAAAGTCTGGACCCATTTCACGTTCCCAGGCCGTCAAGGCAAGTATTCAGATCTAGAGTGGCATTGGTGG
CACTTTGATGCGGTGGATTACAACGCCTATAACGGTGACCAGCAGGCGGTCTATCTCTTTAAGGACAAAAAATTTGATAG
TAATGTTGACTTAGAAAAGGGAGTCTATGATTATCTGATGGGATGTGACCTAGACATGGAACATCCCGAAGTTCAGGACG
CCCTCAAAGCTTGGGGAAAGTGGTTCGTGGAGACCACGCAAGTGAATGGGTTCCGTTTTGACGCCGTCAAGCATGTCAAA
GCAGATTTTTTCCCTGAGTGGCTGAACCATTGTCGGCAGCACTCCCAGAAACGCCTGTTTGCAGTAGGGGAATATTGGTC
TGATGAAATTGAAGCCCTGCACCACTTTGTCAAGGTAACTGGGGGCGATGTCATGCTGTTTGATGCCCCGTTACACTACA
ACTTTACGGTGGCGAGTACGAAAGGGTGTGACTTTGACCTCCGCCAAATTTTTGATGACACGTTGGTGAAAGAGCAGCCT
GCCTTAGCCGTCACCCTGGTTGAAAATCATGATTCTCAGCCCTTGCAGGCGTTAGAGTCTGTGGTGGAGTCTTGGTTTAA
GCCCTTGGCCTATGCCTTGATTTTGTTACGTCGAGATGGATATCCCTGTATTTTCTATGCAGACTATTATGGGGCTCACT
ACAAGGACAAAGGCCGTGATGGTAATGAGTATGAAATTTGGTTGGACAGTCATCAATGGTTAATCGATAAGTTCCTGGGA
GCTCGACGGACCTATGCCTATGGCGATCAGTACGACTATTTTGATCATGCCAACTGTGTGGGCTGGACCCGCTTAGGCAC
GGATGAGCATCCGGGGGGAATGGCCGTCATCCTTAGCAATGGCGATCAGGGCTGCAAATGGATGGAAATGGGGCACCCCC
ACCAAACCTATCGGGATGTGACAGAACATATTTCTGAATCGATCACGACCAATGCCGAGGGTTGGGCTGAATTTGCCTGT
AAGGCTGGCTCCGTCTCTGTATGGGTCCCCCAAACGGATTAG

Upstream 100 bases:

>100_bases
TTTTAATGGCGAATCTATATAGCCTTAATCCAAAAAATCCCACCAATTTATCCCTAACGAATAAAATTGAAATCCCTATC
TTTCCAAGTAGAGGTCATGT

Downstream 100 bases:

>100_bases
CATCGGTTATTTAGATTTAGCCGCTTCTGTTTGCTCAGGTTCTTTCGGATCAATATCCACAAAAGCGCCGTTAAAAAATT
GGGCAAAACTCTTGGCTGCG

Product: cytoplasmic alpha-amylase

Products: NA

Alternate protein names: 1,4-alpha-D-glucan glucanohydrolase [H]

Number of amino acids: Translated: 493; Mature: 492

Protein sequence:

>493_residues
MSEPNGVMMQFFHWYNAADGSLWSELKTKAADLANVGITAVWLPPAYKGLAGGYDVGYGVYDMYDLGEFDQKGSIRTKYG
TKDEYISAIKVAQTVGIHVYADVVLNHKMGADAEEEAQARPMNPDNRHQPIGDTQTVKVWTHFTFPGRQGKYSDLEWHWW
HFDAVDYNAYNGDQQAVYLFKDKKFDSNVDLEKGVYDYLMGCDLDMEHPEVQDALKAWGKWFVETTQVNGFRFDAVKHVK
ADFFPEWLNHCRQHSQKRLFAVGEYWSDEIEALHHFVKVTGGDVMLFDAPLHYNFTVASTKGCDFDLRQIFDDTLVKEQP
ALAVTLVENHDSQPLQALESVVESWFKPLAYALILLRRDGYPCIFYADYYGAHYKDKGRDGNEYEIWLDSHQWLIDKFLG
ARRTYAYGDQYDYFDHANCVGWTRLGTDEHPGGMAVILSNGDQGCKWMEMGHPHQTYRDVTEHISESITTNAEGWAEFAC
KAGSVSVWVPQTD

Sequences:

>Translated_493_residues
MSEPNGVMMQFFHWYNAADGSLWSELKTKAADLANVGITAVWLPPAYKGLAGGYDVGYGVYDMYDLGEFDQKGSIRTKYG
TKDEYISAIKVAQTVGIHVYADVVLNHKMGADAEEEAQARPMNPDNRHQPIGDTQTVKVWTHFTFPGRQGKYSDLEWHWW
HFDAVDYNAYNGDQQAVYLFKDKKFDSNVDLEKGVYDYLMGCDLDMEHPEVQDALKAWGKWFVETTQVNGFRFDAVKHVK
ADFFPEWLNHCRQHSQKRLFAVGEYWSDEIEALHHFVKVTGGDVMLFDAPLHYNFTVASTKGCDFDLRQIFDDTLVKEQP
ALAVTLVENHDSQPLQALESVVESWFKPLAYALILLRRDGYPCIFYADYYGAHYKDKGRDGNEYEIWLDSHQWLIDKFLG
ARRTYAYGDQYDYFDHANCVGWTRLGTDEHPGGMAVILSNGDQGCKWMEMGHPHQTYRDVTEHISESITTNAEGWAEFAC
KAGSVSVWVPQTD
>Mature_492_residues
SEPNGVMMQFFHWYNAADGSLWSELKTKAADLANVGITAVWLPPAYKGLAGGYDVGYGVYDMYDLGEFDQKGSIRTKYGT
KDEYISAIKVAQTVGIHVYADVVLNHKMGADAEEEAQARPMNPDNRHQPIGDTQTVKVWTHFTFPGRQGKYSDLEWHWWH
FDAVDYNAYNGDQQAVYLFKDKKFDSNVDLEKGVYDYLMGCDLDMEHPEVQDALKAWGKWFVETTQVNGFRFDAVKHVKA
DFFPEWLNHCRQHSQKRLFAVGEYWSDEIEALHHFVKVTGGDVMLFDAPLHYNFTVASTKGCDFDLRQIFDDTLVKEQPA
LAVTLVENHDSQPLQALESVVESWFKPLAYALILLRRDGYPCIFYADYYGAHYKDKGRDGNEYEIWLDSHQWLIDKFLGA
RRTYAYGDQYDYFDHANCVGWTRLGTDEHPGGMAVILSNGDQGCKWMEMGHPHQTYRDVTEHISESITTNAEGWAEFACK
AGSVSVWVPQTD

Specific function: Unknown

COG id: COG0366

COG function: function code G; Glycosidases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family [H]

Homologues:

Organism=Escherichia coli, GI1788236, Length=491, Percent_Identity=46.8431771894094, Blast_Score=443, Evalue=1e-125,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013776
- InterPro:   IPR015237
- InterPro:   IPR013780
- InterPro:   IPR006047
- InterPro:   IPR006589
- InterPro:   IPR017853
- InterPro:   IPR013781 [H]

Pfam domain/function: PF00128 Alpha-amylase; PF09154 DUF1939 [H]

EC number: =3.2.1.1 [H]

Molecular weight: Translated: 56460; Mature: 56329

Theoretical pI: Translated: 4.87; Mature: 4.87

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEPNGVMMQFFHWYNAADGSLWSELKTKAADLANVGITAVWLPPAYKGLAGGYDVGYGV
CCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCEEEEECCCCHHCCCCCCCCCCCC
YDMYDLGEFDQKGSIRTKYGTKDEYISAIKVAQTVGIHVYADVVLNHKMGADAEEEAQAR
EEHHCCCCCCCCCCEEECCCCCHHHHHHHHHHHHHCEEEEEEEEEECCCCCCCHHHHHCC
PMNPDNRHQPIGDTQTVKVWTHFTFPGRQGKYSDLEWHWWHFDAVDYNAYNGDQQAVYLF
CCCCCCCCCCCCCCEEEEEEEEEECCCCCCCCCCCEEEEEEEEEEECCCCCCCCCEEEEE
KDKKFDSNVDLEKGVYDYLMGCDLDMEHPEVQDALKAWGKWFVETTQVNGFRFDAVKHVK
ECCCCCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHEEEEEEECCEEEHHHHHHH
ADFFPEWLNHCRQHSQKRLFAVGEYWSDEIEALHHFVKVTGGDVMLFDAPLHYNFTVAST
HHCCHHHHHHHHHHHHHHEEEECHHHHHHHHHHHHHHHCCCCCEEEEECCCEEEEEEECC
KGCDFDLRQIFDDTLVKEQPALAVTLVENHDSQPLQALESVVESWFKPLAYALILLRRDG
CCCCHHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCC
YPCIFYADYYGAHYKDKGRDGNEYEIWLDSHQWLIDKFLGARRTYAYGDQYDYFDHANCV
CCEEEEECCCCCCCCCCCCCCCEEEEEECCCHHHHHHHHCCCCEEECCCCCCCCCCCCCC
GWTRLGTDEHPGGMAVILSNGDQGCKWMEMGHPHQTYRDVTEHISESITTNAEGWAEFAC
EEEECCCCCCCCCEEEEECCCCCCCEEEECCCCHHHHHHHHHHHHHHHCCCCCHHHHHHC
KAGSVSVWVPQTD
CCCCEEEEEECCC
>Mature Secondary Structure 
SEPNGVMMQFFHWYNAADGSLWSELKTKAADLANVGITAVWLPPAYKGLAGGYDVGYGV
CCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCEEEEECCCCHHCCCCCCCCCCCC
YDMYDLGEFDQKGSIRTKYGTKDEYISAIKVAQTVGIHVYADVVLNHKMGADAEEEAQAR
EEHHCCCCCCCCCCEEECCCCCHHHHHHHHHHHHHCEEEEEEEEEECCCCCCCHHHHHCC
PMNPDNRHQPIGDTQTVKVWTHFTFPGRQGKYSDLEWHWWHFDAVDYNAYNGDQQAVYLF
CCCCCCCCCCCCCCEEEEEEEEEECCCCCCCCCCCEEEEEEEEEEECCCCCCCCCEEEEE
KDKKFDSNVDLEKGVYDYLMGCDLDMEHPEVQDALKAWGKWFVETTQVNGFRFDAVKHVK
ECCCCCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHEEEEEEECCEEEHHHHHHH
ADFFPEWLNHCRQHSQKRLFAVGEYWSDEIEALHHFVKVTGGDVMLFDAPLHYNFTVAST
HHCCHHHHHHHHHHHHHHEEEECHHHHHHHHHHHHHHHCCCCCEEEEECCCEEEEEEECC
KGCDFDLRQIFDDTLVKEQPALAVTLVENHDSQPLQALESVVESWFKPLAYALILLRRDG
CCCCHHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCC
YPCIFYADYYGAHYKDKGRDGNEYEIWLDSHQWLIDKFLGARRTYAYGDQYDYFDHANCV
CCEEEEECCCCCCCCCCCCCCCEEEEEECCCHHHHHHHHCCCCEEECCCCCCCCCCCCCC
GWTRLGTDEHPGGMAVILSNGDQGCKWMEMGHPHQTYRDVTEHISESITTNAEGWAEFAC
EEEECCCCCCCCCEEEEECCCCCCCEEEECCCCHHHHHHHHHHHHHHHCCCCCHHHHHHC
KAGSVSVWVPQTD
CCCCEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3924897; 3876333; 3009417; 2999073; 11226887 [H]