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The map label for this gene is hsdS [H]

Identifier: 158335388

GI number: 158335388

Start: 2242037

End: 2243758

Strand: Reverse

Name: hsdS [H]

Synonym: AM1_2233

Alternate gene names: 158335388

Gene position: 2243758-2242037 (Counterclockwise)

Preceding gene: 158335389

Following gene: 158335387

Centisome position: 34.5

GC content: 43.44

Gene sequence:

>1722_bases
ATGGATTTGCCAACATTTTGGGATAATTTCGAGGTGATTGCGGAAGCGCCGGGTGGGGTGCAACGGCTGCGGGAGTTGAT
TTTGGATTTGGCGGTACGGGGAAAGTTAGTGCCGCAAGATCCGACAGAAGGACAAGCTCAAGAACAGCTTGCAAATCTGA
GGAATGAGCGAGCTGAACATTTAGCAAAAACTGCGGATTCCAATACTGAGTATCGCAGAATGTTGAAAAAGCTCTCTGCT
TTAGAATCCTCTGTCCCCCCCTTTGATATACCGTCAAATTGGTCGATAGCACATCTAATTGATTTATCTCTTTTGGTTGT
GGATTGTCATAACAAAACAGCTCCCACCACTTTCGAAGGTATTCCACTAATACGAACGACTAATATCAGAAATCGCCAGT
TCCGTTTTCATGGGATGAAATATGTAGATCAAGACACATATGAGTTTTGGTCACGAAGATGCTTCCCTGAGCCAGGAGAC
ATTATCTTTACGCGCGAAGCTCCAATGGGTGAAGCCACTATTATTCCTGATGGGATGAAAGTTTGTTTGGGACAGCGAAC
AATGCTAATTCGTGTCTTTGAACAGTTTGTAGATCGAAACTTTGTACTGTTAGCGTTGACTGAACCAGGTTTAATCAAGC
GACTTGCTAGTAATGCTGTTGGAATGACAGTTAAGCATCTGCGAGTTAAAGACGTAGAACAAATTTGTCTACCACTACCA
CCTCTTGCAGAGCAGAAGCGGATTGTGGCGAAGGTGGATGAATTGATGGCAATGTGCGATCGCTATGAGGTATCGAAGTG
CGATCGCAACACCCTACGAACAAAAATGCGAGCAAGTGCGAACGATGCCCTGATGAATGCTGAAACAGATGAATCGCTAA
ATACGGCCTGGGAATTTGTTCAAGAGCATTGGGAATGCCTAATTCAGGAACCTGAAGAAGTTGAGACCGTCCGCAAAGTC
ATCTTGCAGCTAGGAGCAAGAGGCAAATTATCGAAACAGACTGTTACTGATGGCTCTGTAGATAAAGTCATTGAGCAGGC
TCAGCATGAAAAAAATGCATTATCAAAAAAGAAGCCGTTTCCCAAACTTAGGAAAATCAAGCCCTTAGATGAAGTAGAAC
AGCCGTTTCAAATTCCAAGGTCGTGGACATGGGTTCGTGTTGAGACAATTTGCACTCATATTGTTGATTGCTTACATCGA
ACACCTAAGTATCAAGAGAATGGTTATCCTGCAATTCGAACTTCAGATATACAGCCAGGTAAAATCCTTGTTGATCAAGC
TCGAAAAGTTGGGATAGAAGAGTATCAAACACAAACTCAAAGACTCATACCACAAGAGGGAGATATTTTTTATTCGAGAG
AGGGAAATTTTGGGATAGCGGCGGTTGTTCCACCGCAATGTGAAATATGTCTTTCACAGCGTATGATGCAATTCCGTGTA
GCTTCGAATATAGATCCCTACTTTTTTTCTTGGGTTATGAATGCTCCAGTCATTTTTAATCAAGCATTAAATGATGCGGC
TGGAATGACTGTCCCTCATGTTAATATTCGTTCCCTTAAGCAATTCGTTTTTCCTCTTCCCCCTTTCGCTGAACAAAAAC
GCATAGTCATCAAAGTCGATCAACTGATGACCTTCTGTGACAACCTCGAAGCCCACCTGCACGAAACCCAGGAAAAAGCC
ACAGCCCTTGCCGCTGCTGTAGTAGGCCAGCTAGAAGTTTAG

Upstream 100 bases:

>100_bases
CTGCGATTGTGTCTGGATGTGATGGTTTTTTGACAAATGATTTTCAGCTGAAGAAGGTGGCTGAGCTGCAGGTTTTGGTT
GTAGATGAGCTGGAAGTCTA

Downstream 100 bases:

>100_bases
GTTTTAGGTCTAAGTGCCACCCCTGTAAGCCCGTTCCCATGAACGATAATCACCCCGATTTGCTATATCAACGTATTCGC
GACATCCTCAACGCCGCCCA

Product: type I restriction-modification system S subunit

Products: NA

Alternate protein names: S.EcoAI; Type I restriction enzyme EcoAI specificity protein; S protein [H]

Number of amino acids: Translated: 573; Mature: 573

Protein sequence:

>573_residues
MDLPTFWDNFEVIAEAPGGVQRLRELILDLAVRGKLVPQDPTEGQAQEQLANLRNERAEHLAKTADSNTEYRRMLKKLSA
LESSVPPFDIPSNWSIAHLIDLSLLVVDCHNKTAPTTFEGIPLIRTTNIRNRQFRFHGMKYVDQDTYEFWSRRCFPEPGD
IIFTREAPMGEATIIPDGMKVCLGQRTMLIRVFEQFVDRNFVLLALTEPGLIKRLASNAVGMTVKHLRVKDVEQICLPLP
PLAEQKRIVAKVDELMAMCDRYEVSKCDRNTLRTKMRASANDALMNAETDESLNTAWEFVQEHWECLIQEPEEVETVRKV
ILQLGARGKLSKQTVTDGSVDKVIEQAQHEKNALSKKKPFPKLRKIKPLDEVEQPFQIPRSWTWVRVETICTHIVDCLHR
TPKYQENGYPAIRTSDIQPGKILVDQARKVGIEEYQTQTQRLIPQEGDIFYSREGNFGIAAVVPPQCEICLSQRMMQFRV
ASNIDPYFFSWVMNAPVIFNQALNDAAGMTVPHVNIRSLKQFVFPLPPFAEQKRIVIKVDQLMTFCDNLEAHLHETQEKA
TALAAAVVGQLEV

Sequences:

>Translated_573_residues
MDLPTFWDNFEVIAEAPGGVQRLRELILDLAVRGKLVPQDPTEGQAQEQLANLRNERAEHLAKTADSNTEYRRMLKKLSA
LESSVPPFDIPSNWSIAHLIDLSLLVVDCHNKTAPTTFEGIPLIRTTNIRNRQFRFHGMKYVDQDTYEFWSRRCFPEPGD
IIFTREAPMGEATIIPDGMKVCLGQRTMLIRVFEQFVDRNFVLLALTEPGLIKRLASNAVGMTVKHLRVKDVEQICLPLP
PLAEQKRIVAKVDELMAMCDRYEVSKCDRNTLRTKMRASANDALMNAETDESLNTAWEFVQEHWECLIQEPEEVETVRKV
ILQLGARGKLSKQTVTDGSVDKVIEQAQHEKNALSKKKPFPKLRKIKPLDEVEQPFQIPRSWTWVRVETICTHIVDCLHR
TPKYQENGYPAIRTSDIQPGKILVDQARKVGIEEYQTQTQRLIPQEGDIFYSREGNFGIAAVVPPQCEICLSQRMMQFRV
ASNIDPYFFSWVMNAPVIFNQALNDAAGMTVPHVNIRSLKQFVFPLPPFAEQKRIVIKVDQLMTFCDNLEAHLHETQEKA
TALAAAVVGQLEV
>Mature_573_residues
MDLPTFWDNFEVIAEAPGGVQRLRELILDLAVRGKLVPQDPTEGQAQEQLANLRNERAEHLAKTADSNTEYRRMLKKLSA
LESSVPPFDIPSNWSIAHLIDLSLLVVDCHNKTAPTTFEGIPLIRTTNIRNRQFRFHGMKYVDQDTYEFWSRRCFPEPGD
IIFTREAPMGEATIIPDGMKVCLGQRTMLIRVFEQFVDRNFVLLALTEPGLIKRLASNAVGMTVKHLRVKDVEQICLPLP
PLAEQKRIVAKVDELMAMCDRYEVSKCDRNTLRTKMRASANDALMNAETDESLNTAWEFVQEHWECLIQEPEEVETVRKV
ILQLGARGKLSKQTVTDGSVDKVIEQAQHEKNALSKKKPFPKLRKIKPLDEVEQPFQIPRSWTWVRVETICTHIVDCLHR
TPKYQENGYPAIRTSDIQPGKILVDQARKVGIEEYQTQTQRLIPQEGDIFYSREGNFGIAAVVPPQCEICLSQRMMQFRV
ASNIDPYFFSWVMNAPVIFNQALNDAAGMTVPHVNIRSLKQFVFPLPPFAEQKRIVIKVDQLMTFCDNLEAHLHETQEKA
TALAAAVVGQLEV

Specific function: The M and S subunits together form a methyltransferase (MTase) that methylates two adenine residues in complementary strands of a bipartite DNA recognition sequence. In the presence of the R subunit the complex can also act as an endonuclease, binding to

COG id: COG0732

COG function: function code V; Restriction endonuclease S subunits

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the type-I restriction system S methylase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000055 [H]

Pfam domain/function: PF01420 Methylase_S [H]

EC number: NA

Molecular weight: Translated: 65373; Mature: 65373

Theoretical pI: Translated: 6.65; Mature: 6.65

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDLPTFWDNFEVIAEAPGGVQRLRELILDLAVRGKLVPQDPTEGQAQEQLANLRNERAEH
CCCCCCCCCCCEEECCCCHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHH
LAKTADSNTEYRRMLKKLSALESSVPPFDIPSNWSIAHLIDLSLLVVDCHNKTAPTTFEG
HHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHEEEEEEECCCCCCCCCCCC
IPLIRTTNIRNRQFRFHGMKYVDQDTYEFWSRRCFPEPGDIIFTREAPMGEATIIPDGMK
CCEEEECCCCCCEEEECCCHHCCCHHHHHHHHCCCCCCCCEEEECCCCCCCEEECCCHHH
VCLGQRTMLIRVFEQFVDRNFVLLALTEPGLIKRLASNAVGMTVKHLRVKDVEQICLPLP
HHHCCHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
PLAEQKRIVAKVDELMAMCDRYEVSKCDRNTLRTKMRASANDALMNAETDESLNTAWEFV
CCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCHHHCCCCCCHHHHHHHHHH
QEHWECLIQEPEEVETVRKVILQLGARGKLSKQTVTDGSVDKVIEQAQHEKNALSKKKPF
HHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCC
PKLRKIKPLDEVEQPFQIPRSWTWVRVETICTHIVDCLHRTPKYQENGYPAIRTSDIQPG
CHHHCCCCHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHCCCCCCCCCCCCEEECCCCCC
KILVDQARKVGIEEYQTQTQRLIPQEGDIFYSREGNFGIAAVVPPQCEICLSQRMMQFRV
HHHHHHHHHCCHHHHHHHHHHCCCCCCCEEEECCCCEEEEEECCCHHHHHHHHHHHHHHH
ASNIDPYFFSWVMNAPVIFNQALNDAAGMTVPHVNIRSLKQFVFPLPPFAEQKRIVIKVD
HCCCCHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCCCCCCCEEEEEHH
QLMTFCDNLEAHLHETQEKATALAAAVVGQLEV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MDLPTFWDNFEVIAEAPGGVQRLRELILDLAVRGKLVPQDPTEGQAQEQLANLRNERAEH
CCCCCCCCCCCEEECCCCHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHH
LAKTADSNTEYRRMLKKLSALESSVPPFDIPSNWSIAHLIDLSLLVVDCHNKTAPTTFEG
HHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHEEEEEEECCCCCCCCCCCC
IPLIRTTNIRNRQFRFHGMKYVDQDTYEFWSRRCFPEPGDIIFTREAPMGEATIIPDGMK
CCEEEECCCCCCEEEECCCHHCCCHHHHHHHHCCCCCCCCEEEECCCCCCCEEECCCHHH
VCLGQRTMLIRVFEQFVDRNFVLLALTEPGLIKRLASNAVGMTVKHLRVKDVEQICLPLP
HHHCCHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
PLAEQKRIVAKVDELMAMCDRYEVSKCDRNTLRTKMRASANDALMNAETDESLNTAWEFV
CCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCHHHCCCCCCHHHHHHHHHH
QEHWECLIQEPEEVETVRKVILQLGARGKLSKQTVTDGSVDKVIEQAQHEKNALSKKKPF
HHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCC
PKLRKIKPLDEVEQPFQIPRSWTWVRVETICTHIVDCLHRTPKYQENGYPAIRTSDIQPG
CHHHCCCCHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHCCCCCCCCCCCCEEECCCCCC
KILVDQARKVGIEEYQTQTQRLIPQEGDIFYSREGNFGIAAVVPPQCEICLSQRMMQFRV
HHHHHHHHHCCHHHHHHHHHHCCCCCCCEEEECCCCEEEEEECCCHHHHHHHHHHHHHHH
ASNIDPYFFSWVMNAPVIFNQALNDAAGMTVPHVNIRSLKQFVFPLPPFAEQKRIVIKVD
HCCCCHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCCCCCCCEEEEEHH
QLMTFCDNLEAHLHETQEKATALAAAVVGQLEV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: Hydrolase; Acting on ester bonds [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2642743 [H]