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The map label for this gene is purD [H]

Identifier: 158335328

GI number: 158335328

Start: 2172546

End: 2173829

Strand: Reverse

Name: purD [H]

Synonym: AM1_2172

Alternate gene names: 158335328

Gene position: 2173829-2172546 (Counterclockwise)

Preceding gene: 158335329

Following gene: 158335324

Centisome position: 33.42

GC content: 50.16

Gene sequence:

>1284_bases
GTGAATATTTTAGTGATTGGCAGTGGTGGTCGCGAGCATGCATTGGCGTGGTCCTTGTTGCGATCGCAAACGGTGCAGCA
AGTGTATTGTTTACCTGGCAACGGGGGTACTGCTACCCTAACCAACTGTCAAAATATTGATATTTCTGAGCAAGACTTTG
CTGCTATTGGTCAATTTGCCCAATCCCAGGACATCGCCTTAGTAGTTGTGGGGCCAGAAGCACCTCTGGCTGAGGGAATT
ACGGATGCCCTGCAGCCGTTTAACATCCCTGTATTTGGCCCTACCCAAGCTGGTGCCCAACTCGAAGCCAGCAAAACCTG
GGCCAAAACCCTCATGACCGAGGCTGGTATCCCTACGGCTAAGGCCCAAACCTTTACCGATGTAGATGCTGCTCAGGCTT
ATATTCAGTCGGAAGGTGCTCCAATTGTGATTAAGGCAGATGGTCTGGCTGCAGGCAAAGGAGTGACCGTTGCTCTCACC
CTAGATGAAGCATTGAATGCTGTGAATGAGGCCTTCTCCGGTAAATTTGGCGAAGCGGGCCAGACCGTTTTAATTGAGGA
ATATCTAACAGGGCAAGAAGTTTCAGTTTTAGCTTTGACGGATGGCAAAACCATTAAACCGTTGATTCCAGCTCAAGACC
ATAAGCCCATTGGCGAAGGAGATACGGGGCCAAATACCGGCGGTATGGGAGCCTATGCGCCGGTTCCCTGGGTTACCCCT
AAATTAATGCAGCGTGTAGAAACAGACATTCTTCAACCTACTCTAACGGCACTGCAGCAGAAAGGCATTGATTATCGCGG
TGTGGTCTATGCAGGGCTGATCATCACTCCTACCGGTGAGCCCAAAGTCATCGAATTTAACTGTCGATTTGGCGATCCCG
AGACCCAAGCAGTATTAAGTCTTCTAGCCACCCCGTTGGATCAGCTCTTGATGGCCTGTACCCAACAAGAACTGGCTGAA
ATTGATATCCAATGGCAGTCAGGCTTCTCAGCCTGTGTCGTGTTGGCCTCAGGAGGGTATCCCGGTAGCTACCAAAAGGG
CTTCCCAATTGCCGATCTTGATAAAGCCGAAGCCCAGAACGTCACCGTCTTTCATGCTGGCACCCAACTTAAAGACCAAG
CCATTATCACCAGTGGCGGCAGGGTCTTAGCGGTGACTGGACAAGGGAGCACCTTTGAAGAAGCGCTAAAAACGGTTTAT
CAGGCGATACCGTCCATTCAATTTGAGGGCTGCTATTACCGCCACGATATTGGTCAGCGCACCATTAACATCAATTTGGA
TTAA

Upstream 100 bases:

>100_bases
CTGTTTCACCTGTTCAAAGTCAAGTTTTAACCTGGCTGTTGACCAATGCTACTAGCGTCACCCAGACTATAGGATGTTCT
CTGTTGGGTAGACGTTAGCC

Downstream 100 bases:

>100_bases
GCCGACTAGAAGGGAATGTCATCGTAATTGGGTTCTGCTTCTGCTGGAGCTGGCGCCGGCGGTGCAGGAATGGCTGCAGG
TGTTGCCGCTGCTGGAGCGG

Product: phosphoribosylamine--glycine ligase

Products: NA

Alternate protein names: GARS; Glycinamide ribonucleotide synthetase; Phosphoribosylglycinamide synthetase [H]

Number of amino acids: Translated: 427; Mature: 427

Protein sequence:

>427_residues
MNILVIGSGGREHALAWSLLRSQTVQQVYCLPGNGGTATLTNCQNIDISEQDFAAIGQFAQSQDIALVVVGPEAPLAEGI
TDALQPFNIPVFGPTQAGAQLEASKTWAKTLMTEAGIPTAKAQTFTDVDAAQAYIQSEGAPIVIKADGLAAGKGVTVALT
LDEALNAVNEAFSGKFGEAGQTVLIEEYLTGQEVSVLALTDGKTIKPLIPAQDHKPIGEGDTGPNTGGMGAYAPVPWVTP
KLMQRVETDILQPTLTALQQKGIDYRGVVYAGLIITPTGEPKVIEFNCRFGDPETQAVLSLLATPLDQLLMACTQQELAE
IDIQWQSGFSACVVLASGGYPGSYQKGFPIADLDKAEAQNVTVFHAGTQLKDQAIITSGGRVLAVTGQGSTFEEALKTVY
QAIPSIQFEGCYYRHDIGQRTININLD

Sequences:

>Translated_427_residues
MNILVIGSGGREHALAWSLLRSQTVQQVYCLPGNGGTATLTNCQNIDISEQDFAAIGQFAQSQDIALVVVGPEAPLAEGI
TDALQPFNIPVFGPTQAGAQLEASKTWAKTLMTEAGIPTAKAQTFTDVDAAQAYIQSEGAPIVIKADGLAAGKGVTVALT
LDEALNAVNEAFSGKFGEAGQTVLIEEYLTGQEVSVLALTDGKTIKPLIPAQDHKPIGEGDTGPNTGGMGAYAPVPWVTP
KLMQRVETDILQPTLTALQQKGIDYRGVVYAGLIITPTGEPKVIEFNCRFGDPETQAVLSLLATPLDQLLMACTQQELAE
IDIQWQSGFSACVVLASGGYPGSYQKGFPIADLDKAEAQNVTVFHAGTQLKDQAIITSGGRVLAVTGQGSTFEEALKTVY
QAIPSIQFEGCYYRHDIGQRTININLD
>Mature_427_residues
MNILVIGSGGREHALAWSLLRSQTVQQVYCLPGNGGTATLTNCQNIDISEQDFAAIGQFAQSQDIALVVVGPEAPLAEGI
TDALQPFNIPVFGPTQAGAQLEASKTWAKTLMTEAGIPTAKAQTFTDVDAAQAYIQSEGAPIVIKADGLAAGKGVTVALT
LDEALNAVNEAFSGKFGEAGQTVLIEEYLTGQEVSVLALTDGKTIKPLIPAQDHKPIGEGDTGPNTGGMGAYAPVPWVTP
KLMQRVETDILQPTLTALQQKGIDYRGVVYAGLIITPTGEPKVIEFNCRFGDPETQAVLSLLATPLDQLLMACTQQELAE
IDIQWQSGFSACVVLASGGYPGSYQKGFPIADLDKAEAQNVTVFHAGTQLKDQAIITSGGRVLAVTGQGSTFEEALKTVY
QAIPSIQFEGCYYRHDIGQRTININLD

Specific function: De novo purine biosynthesis; second step. [C]

COG id: COG0151

COG function: function code F; Phosphoribosylamine-glycine ligase

Gene ontology:
GO:0003824: Phosphoribosylamine--glycine ligase
GO:0004637: Phosphoribosylamine--glycine ligase
GO:0005524: Phosphoribosylamine--glycine ligase
GO:0006164: Phosphoribosylamine--glycine ligase
GO:0009113: Phosphoribosylamine--glycine ligase
GO:0016874: Phosphoribosylamine--glycine ligase
GO:0046872: Phosphoribosylamine--glycine ligase

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=424, Percent_Identity=48.8207547169811, Blast_Score=388, Evalue=1e-108,
Organism=Homo sapiens, GI209869995, Length=424, Percent_Identity=48.8207547169811, Blast_Score=388, Evalue=1e-108,
Organism=Homo sapiens, GI209869993, Length=424, Percent_Identity=48.8207547169811, Blast_Score=388, Evalue=1e-108,
Organism=Homo sapiens, GI28416899, Length=424, Percent_Identity=48.8207547169811, Blast_Score=387, Evalue=1e-108,
Organism=Escherichia coli, GI1790438, Length=424, Percent_Identity=48.3490566037736, Blast_Score=405, Evalue=1e-114,
Organism=Caenorhabditis elegans, GI17567511, Length=430, Percent_Identity=44.4186046511628, Blast_Score=340, Evalue=8e-94,
Organism=Saccharomyces cerevisiae, GI6321203, Length=438, Percent_Identity=43.8356164383562, Blast_Score=338, Evalue=1e-93,
Organism=Drosophila melanogaster, GI24582400, Length=432, Percent_Identity=47.6851851851852, Blast_Score=372, Evalue=1e-103,
Organism=Drosophila melanogaster, GI62471619, Length=432, Percent_Identity=47.4537037037037, Blast_Score=370, Evalue=1e-102,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR020561
- InterPro:   IPR000115
- InterPro:   IPR020560
- InterPro:   IPR020559
- InterPro:   IPR020562
- InterPro:   IPR011054 [H]

Pfam domain/function: PF01071 GARS_A; PF02843 GARS_C; PF02844 GARS_N [H]

EC number: =6.3.4.13 [H]

Molecular weight: Translated: 45142; Mature: 45142

Theoretical pI: Translated: 4.23; Mature: 4.23

Prosite motif: PS50975 ATP_GRASP ; PS00184 GARS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNILVIGSGGREHALAWSLLRSQTVQQVYCLPGNGGTATLTNCQNIDISEQDFAAIGQFA
CEEEEECCCCCCHHHHHHHHHHCCCEEEEEECCCCCEEEEECCCCCCCCHHHHHHHHHHC
QSQDIALVVVGPEAPLAEGITDALQPFNIPVFGPTQAGAQLEASKTWAKTLMTEAGIPTA
CCCCEEEEEECCCCCHHHHHHHHHCCCCCEEECCCCCCCEEEHHHHHHHHHHHHCCCCCC
KAQTFTDVDAAQAYIQSEGAPIVIKADGLAAGKGVTVALTLDEALNAVNEAFSGKFGEAG
CCCCCCCHHHHHHHHHCCCCEEEEEECCCCCCCCEEEEEEHHHHHHHHHHHHCCCCCCCC
QTVLIEEYLTGQEVSVLALTDGKTIKPLIPAQDHKPIGEGDTGPNTGGMGAYAPVPWVTP
CEEEEEECCCCCCEEEEEEECCCEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCH
KLMQRVETDILQPTLTALQQKGIDYRGVVYAGLIITPTGEPKVIEFNCRFGDPETQAVLS
HHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEEECCCCCEEEEEEECCCCCHHHHHHH
LLATPLDQLLMACTQQELAEIDIQWQSGFSACVVLASGGYPGSYQKGFPIADLDKAEAQN
HHHCCHHHHHHHHHHHHHHEEEEEECCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCC
VTVFHAGTQLKDQAIITSGGRVLAVTGQGSTFEEALKTVYQAIPSIQFEGCYYRHDIGQR
EEEEECCCCCCCCEEEECCCEEEEEECCCCCHHHHHHHHHHHCCCEEECCEEEEECCCCE
TININLD
EEEEEEC
>Mature Secondary Structure
MNILVIGSGGREHALAWSLLRSQTVQQVYCLPGNGGTATLTNCQNIDISEQDFAAIGQFA
CEEEEECCCCCCHHHHHHHHHHCCCEEEEEECCCCCEEEEECCCCCCCCHHHHHHHHHHC
QSQDIALVVVGPEAPLAEGITDALQPFNIPVFGPTQAGAQLEASKTWAKTLMTEAGIPTA
CCCCEEEEEECCCCCHHHHHHHHHCCCCCEEECCCCCCCEEEHHHHHHHHHHHHCCCCCC
KAQTFTDVDAAQAYIQSEGAPIVIKADGLAAGKGVTVALTLDEALNAVNEAFSGKFGEAG
CCCCCCCHHHHHHHHHCCCCEEEEEECCCCCCCCEEEEEEHHHHHHHHHHHHCCCCCCCC
QTVLIEEYLTGQEVSVLALTDGKTIKPLIPAQDHKPIGEGDTGPNTGGMGAYAPVPWVTP
CEEEEEECCCCCCEEEEEEECCCEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCH
KLMQRVETDILQPTLTALQQKGIDYRGVVYAGLIITPTGEPKVIEFNCRFGDPETQAVLS
HHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEEECCCCCEEEEEEECCCCCHHHHHHH
LLATPLDQLLMACTQQELAEIDIQWQSGFSACVVLASGGYPGSYQKGFPIADLDKAEAQN
HHHCCHHHHHHHHHHHHHHEEEEEECCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCC
VTVFHAGTQLKDQAIITSGGRVLAVTGQGSTFEEALKTVYQAIPSIQFEGCYYRHDIGQR
EEEEECCCCCCCCEEEECCCEEEEEECCCCCHHHHHHHHHHHCCCEEECCEEEEECCCCE
TININLD
EEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11759840 [H]