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The map label for this gene is dnaK [H]

Identifier: 158335214

GI number: 158335214

Start: 2051207

End: 2052805

Strand: Reverse

Name: dnaK [H]

Synonym: AM1_2057

Alternate gene names: 158335214

Gene position: 2052805-2051207 (Counterclockwise)

Preceding gene: 158335218

Following gene: 158335212

Centisome position: 31.56

GC content: 50.34

Gene sequence:

>1599_bases
TTGAGTTACGCAATAGATTTTGGTACCAGTAATACCGTTATTGCTCGTTGGAACCCGGTTACAGAGCAATCTGAAATTAT
TAATCTGCCCAGTTTGAGCTATCAGACGGCCCAAAATCCTGACCTTATCCCCAGTCTGCTCTATGTAGAGCAAGCGGCGG
AGAATAAAGTCTGTGTGGGGCAACAGGTGCGAGATCGCGGCTTGGATATCCAAAATGATCCGCGCTTTTTTCGCAGTTTT
AAACGGGGAATTGGGTCCAAGGTCCAAGGGTTTTTGCCGGAACTGGATGGCCAAACCATTCAGTTTGAACAGGTGGGACG
CTGGTTTTTGCAAACGATTCTCCAGCAGCTCCAAACCACCCATCCGGACGTGAAAGAGTCCTTAGTTCTGACGGTTCCAG
TCGATAGCTTTGAAACCTATCGCCATTGGCTGGGGCAGGTCTGCCAAGATCTGCAGGTGGAGCAGGTGCGGATGATCGAT
GAACCCACCGCCGCAGCCTTAGGCTATGGCAAAGCCGATCAAGATGTGCTGCTGGTGGTGGATTTTGGAGGCGGTACCTT
CGATCTGTCTCTGGTCCAGATCAATACGTCGCAACAAAAGACCCAGCCTTTGGGCTTCATTCTGAAGTGGGGCGATAAGA
ATCTAGCGGATACCTCAAAACAACGACCGCAATCAGCCCGCGTCTTGGCGAAAGCGGGTCAGAACTTGGGGGGCACCGAT
ATTGATAATTGGCTCGTCGATCACTTTGCCAAAACGCAAAACTTAGCCGTCACTCCCCTGACGACTCGCTTAGCCGAGCG
GCTCAAAATTCAACTCTCCCTACAGCCCAAGGCGACCGAGGTTTACTTTAATGAGGAAACCTTTGATAGTTATGAGCTTG
CTTTGGATCGAGAACAATTTGATCAAATTCTCAAGGAGCATCAGTTCTTTGATCGTTTAGATGACAGCATGACCCAGGTC
TTGCAACAGGCTCGTCGCCAAGGTTTAGAGGTTGATGATATTAAAGCCGTTCTGCTGGTGGGGGGAACCAGTCAGATTCC
TGCAGTTCAAACCTGGGTGCAGCAATATTTTGATGACAGCCGCATTTGCAGTAGCCGCCCCTTTGAAGCGATTGCCCAAG
GCGCTTTACAGGTAGCCCAGGGTTTAGAGGTGAAAGATTTTCTCTACCACAGCTACGGCGTTCGCTACTGGGACCGCCGT
CAACAGGCCCATAGTTGGCATCCGATTATTCCTGCAGGCTTGCCCTATCCCATGGAGGAACCGATTGAACTGACCCTCGG
GGCTTCAACGGATGCTCAGCCTAGCATTGAACTCATCTTGGGAGAGTTGGGTAATACCAATAGTGGCACTGAAGTCTTTT
TCGATGGTGATCGCTTAGTAACTCGGCAATTATCTGAATCCCAAACCCTGGTTCAACCTTTGAATGATCGGGATGGGTCA
CGGAAAGTGGCGAAGTTAACACCGCCGGGGTATCCGGGCAGCGATCGCATCAAAGTACTGTTTTGGGTCGATAATCGACG
CACCTTGCGCATCAGCGTTGAAGATCTGTTAACTCAAGATACCCTGCTCAAAAACCAGGTGGTTGCCCAACTCAGCTAA

Upstream 100 bases:

>100_bases
TCGTACGTACCTTTTTCCTCACCATGTTGGTCTGGAATGCTTCGTTGTGTAGGCGTGTTCTCAAACGTTATCCATCTCTT
GTCAGTGAGCGGTCAGTCTC

Downstream 100 bases:

>100_bases
GGCTAGACAGATCAAATGCCCCAGGGGGGGTGATAACTCCGAAGAGTTCCCCTGAGGCAATTTCATCCTAACTTTCTAGT
CTTGAGGATTACCTGAGTGG

Product: DnaK family protein

Products: NA

Alternate protein names: HSP70; Heat shock 70 kDa protein; Heat shock protein 70 [H]

Number of amino acids: Translated: 532; Mature: 531

Protein sequence:

>532_residues
MSYAIDFGTSNTVIARWNPVTEQSEIINLPSLSYQTAQNPDLIPSLLYVEQAAENKVCVGQQVRDRGLDIQNDPRFFRSF
KRGIGSKVQGFLPELDGQTIQFEQVGRWFLQTILQQLQTTHPDVKESLVLTVPVDSFETYRHWLGQVCQDLQVEQVRMID
EPTAAALGYGKADQDVLLVVDFGGGTFDLSLVQINTSQQKTQPLGFILKWGDKNLADTSKQRPQSARVLAKAGQNLGGTD
IDNWLVDHFAKTQNLAVTPLTTRLAERLKIQLSLQPKATEVYFNEETFDSYELALDREQFDQILKEHQFFDRLDDSMTQV
LQQARRQGLEVDDIKAVLLVGGTSQIPAVQTWVQQYFDDSRICSSRPFEAIAQGALQVAQGLEVKDFLYHSYGVRYWDRR
QQAHSWHPIIPAGLPYPMEEPIELTLGASTDAQPSIELILGELGNTNSGTEVFFDGDRLVTRQLSESQTLVQPLNDRDGS
RKVAKLTPPGYPGSDRIKVLFWVDNRRTLRISVEDLLTQDTLLKNQVVAQLS

Sequences:

>Translated_532_residues
MSYAIDFGTSNTVIARWNPVTEQSEIINLPSLSYQTAQNPDLIPSLLYVEQAAENKVCVGQQVRDRGLDIQNDPRFFRSF
KRGIGSKVQGFLPELDGQTIQFEQVGRWFLQTILQQLQTTHPDVKESLVLTVPVDSFETYRHWLGQVCQDLQVEQVRMID
EPTAAALGYGKADQDVLLVVDFGGGTFDLSLVQINTSQQKTQPLGFILKWGDKNLADTSKQRPQSARVLAKAGQNLGGTD
IDNWLVDHFAKTQNLAVTPLTTRLAERLKIQLSLQPKATEVYFNEETFDSYELALDREQFDQILKEHQFFDRLDDSMTQV
LQQARRQGLEVDDIKAVLLVGGTSQIPAVQTWVQQYFDDSRICSSRPFEAIAQGALQVAQGLEVKDFLYHSYGVRYWDRR
QQAHSWHPIIPAGLPYPMEEPIELTLGASTDAQPSIELILGELGNTNSGTEVFFDGDRLVTRQLSESQTLVQPLNDRDGS
RKVAKLTPPGYPGSDRIKVLFWVDNRRTLRISVEDLLTQDTLLKNQVVAQLS
>Mature_531_residues
SYAIDFGTSNTVIARWNPVTEQSEIINLPSLSYQTAQNPDLIPSLLYVEQAAENKVCVGQQVRDRGLDIQNDPRFFRSFK
RGIGSKVQGFLPELDGQTIQFEQVGRWFLQTILQQLQTTHPDVKESLVLTVPVDSFETYRHWLGQVCQDLQVEQVRMIDE
PTAAALGYGKADQDVLLVVDFGGGTFDLSLVQINTSQQKTQPLGFILKWGDKNLADTSKQRPQSARVLAKAGQNLGGTDI
DNWLVDHFAKTQNLAVTPLTTRLAERLKIQLSLQPKATEVYFNEETFDSYELALDREQFDQILKEHQFFDRLDDSMTQVL
QQARRQGLEVDDIKAVLLVGGTSQIPAVQTWVQQYFDDSRICSSRPFEAIAQGALQVAQGLEVKDFLYHSYGVRYWDRRQ
QAHSWHPIIPAGLPYPMEEPIELTLGASTDAQPSIELILGELGNTNSGTEVFFDGDRLVTRQLSESQTLVQPLNDRDGSR
KVAKLTPPGYPGSDRIKVLFWVDNRRTLRISVEDLLTQDTLLKNQVVAQLS

Specific function: Acts as a chaperone [H]

COG id: COG0443

COG function: function code O; Molecular chaperone

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the heat shock protein 70 family [H]

Homologues:

Organism=Homo sapiens, GI24234688, Length=405, Percent_Identity=27.6543209876543, Blast_Score=86, Evalue=7e-17,
Organism=Homo sapiens, GI34419635, Length=264, Percent_Identity=29.5454545454545, Blast_Score=80, Evalue=3e-15,
Organism=Homo sapiens, GI16507237, Length=443, Percent_Identity=25.2821670428894, Blast_Score=80, Evalue=4e-15,
Organism=Escherichia coli, GI1786196, Length=428, Percent_Identity=27.803738317757, Blast_Score=93, Evalue=3e-20,
Organism=Escherichia coli, GI1788875, Length=404, Percent_Identity=23.5148514851485, Blast_Score=76, Evalue=7e-15,
Organism=Caenorhabditis elegans, GI17534015, Length=452, Percent_Identity=25.4424778761062, Blast_Score=94, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17534013, Length=452, Percent_Identity=25.4424778761062, Blast_Score=94, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI32564617, Length=392, Percent_Identity=24.4897959183673, Blast_Score=88, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17534771, Length=311, Percent_Identity=27.3311897106109, Blast_Score=79, Evalue=6e-15,
Organism=Caenorhabditis elegans, GI17568549, Length=328, Percent_Identity=26.219512195122, Blast_Score=75, Evalue=6e-14,
Organism=Saccharomyces cerevisiae, GI6320805, Length=444, Percent_Identity=26.3513513513513, Blast_Score=95, Evalue=3e-20,
Organism=Saccharomyces cerevisiae, GI6322505, Length=448, Percent_Identity=25.8928571428571, Blast_Score=89, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6322426, Length=480, Percent_Identity=25.4166666666667, Blast_Score=84, Evalue=5e-17,
Organism=Saccharomyces cerevisiae, GI6323401, Length=503, Percent_Identity=23.4592445328032, Blast_Score=80, Evalue=6e-16,
Organism=Saccharomyces cerevisiae, GI6325151, Length=471, Percent_Identity=23.1422505307856, Blast_Score=74, Evalue=5e-14,

Paralogues:

None

Copy number: 1300 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 320 Molecules/Cell In: Early Stationary Phase, Rich Media (Based on E. coli). 500 Molecules/Cell In: Early Stationary Phase, Rich Media (Based on E. coli). 20 Molecules/Cell In: Stati

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012725
- InterPro:   IPR018181
- InterPro:   IPR001023
- InterPro:   IPR013126 [H]

Pfam domain/function: PF00012 HSP70 [H]

EC number: NA

Molecular weight: Translated: 59999; Mature: 59868

Theoretical pI: Translated: 4.70; Mature: 4.70

Prosite motif: PS00329 HSP70_2 ; PS01036 HSP70_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSYAIDFGTSNTVIARWNPVTEQSEIINLPSLSYQTAQNPDLIPSLLYVEQAAENKVCVG
CCEEEECCCCCEEEEECCCCCCHHHEECCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCC
QQVRDRGLDIQNDPRFFRSFKRGIGSKVQGFLPELDGQTIQFEQVGRWFLQTILQQLQTT
HHHHHCCCCCCCCHHHHHHHHHCCCCHHHHHCCCCCCCEEEHHHHHHHHHHHHHHHHHCC
HPDVKESLVLTVPVDSFETYRHWLGQVCQDLQVEQVRMIDEPTAAALGYGKADQDVLLVV
CCCCCCCEEEEEECCHHHHHHHHHHHHHHHCCHHHHHHCCCCCHHHHCCCCCCCCEEEEE
DFGGGTFDLSLVQINTSQQKTQPLGFILKWGDKNLADTSKQRPQSARVLAKAGQNLGGTD
ECCCCEEEEEEEEEECCHHHCCCCEEEEEECCCCCCCCHHCCCHHHHHHHHCCCCCCCCC
IDNWLVDHFAKTQNLAVTPLTTRLAERLKIQLSLQPKATEVYFNEETFDSYELALDREQF
HHHHHHHHHHHCCCEEECHHHHHHHHHEEEEEEECCCCEEEEECCCCCCHHHHEECHHHH
DQILKEHQFFDRLDDSMTQVLQQARRQGLEVDDIKAVLLVGGTSQIPAVQTWVQQYFDDS
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCEEEEEECCCCCCHHHHHHHHHHCCCC
RICSSRPFEAIAQGALQVAQGLEVKDFLYHSYGVRYWDRRQQAHSWHPIIPAGLPYPMEE
CCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHCCHHHHHHHHCCCCCCCCCCCCCCCCCC
PIELTLGASTDAQPSIELILGELGNTNSGTEVFFDGDRLVTRQLSESQTLVQPLNDRDGS
CEEEEECCCCCCCCCCEEEEECCCCCCCCCEEEECCCHHHHHHHHHHHHHHHCCCCCCCC
RKVAKLTPPGYPGSDRIKVLFWVDNRRTLRISVEDLLTQDTLLKNQVVAQLS
CEEEEECCCCCCCCCCEEEEEEECCCEEEEEEHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SYAIDFGTSNTVIARWNPVTEQSEIINLPSLSYQTAQNPDLIPSLLYVEQAAENKVCVG
CEEEECCCCCEEEEECCCCCCHHHEECCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCC
QQVRDRGLDIQNDPRFFRSFKRGIGSKVQGFLPELDGQTIQFEQVGRWFLQTILQQLQTT
HHHHHCCCCCCCCHHHHHHHHHCCCCHHHHHCCCCCCCEEEHHHHHHHHHHHHHHHHHCC
HPDVKESLVLTVPVDSFETYRHWLGQVCQDLQVEQVRMIDEPTAAALGYGKADQDVLLVV
CCCCCCCEEEEEECCHHHHHHHHHHHHHHHCCHHHHHHCCCCCHHHHCCCCCCCCEEEEE
DFGGGTFDLSLVQINTSQQKTQPLGFILKWGDKNLADTSKQRPQSARVLAKAGQNLGGTD
ECCCCEEEEEEEEEECCHHHCCCCEEEEEECCCCCCCCHHCCCHHHHHHHHCCCCCCCCC
IDNWLVDHFAKTQNLAVTPLTTRLAERLKIQLSLQPKATEVYFNEETFDSYELALDREQF
HHHHHHHHHHHCCCEEECHHHHHHHHHEEEEEEECCCCEEEEECCCCCCHHHHEECHHHH
DQILKEHQFFDRLDDSMTQVLQQARRQGLEVDDIKAVLLVGGTSQIPAVQTWVQQYFDDS
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCEEEEEECCCCCCHHHHHHHHHHCCCC
RICSSRPFEAIAQGALQVAQGLEVKDFLYHSYGVRYWDRRQQAHSWHPIIPAGLPYPMEE
CCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHCCHHHHHHHHCCCCCCCCCCCCCCCCCC
PIELTLGASTDAQPSIELILGELGNTNSGTEVFFDGDRLVTRQLSESQTLVQPLNDRDGS
CEEEEECCCCCCCCCCEEEEECCCCCCCCCEEEECCCHHHHHHHHHHHHHHHCCCCCCCC
RKVAKLTPPGYPGSDRIKVLFWVDNRRTLRISVEDLLTQDTLLKNQVVAQLS
CEEEEECCCCCCCCCCEEEEEEECCCEEEEEEHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA