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The map label for this gene is hisH

Identifier: 158335206

GI number: 158335206

Start: 2042941

End: 2043585

Strand: Direct

Name: hisH

Synonym: AM1_2049

Alternate gene names: 158335206

Gene position: 2042941-2043585 (Clockwise)

Preceding gene: 158335205

Following gene: 158335207

Centisome position: 31.41

GC content: 52.25

Gene sequence:

>645_bases
ATGCCTATCATCGCCGTGATTGACTACGATATGGGCAATCTGCATTCAGCCTGCAAAGGACTGCAAGAAGCTGGGACTCA
AACAATTGTCAGCGATCGGCCGGAGGATCTGGTATCAGCGGATGCGGTGGTTCTACCCGGCGTAGGTGCTTTTGATCCAG
CGATGCAGCATTTGCGATCTCGCCAGCTGATTCCTGTCATCCAAGATATTCTCGCTAGCGGCAAACCCTTTCTCGGCATT
TGCTTGGGGCTACAAATTTTGTTTGAAGGCAGTGAAGAAGGGACTGAAGCCGGATTAGGCATTATTCCCGGTACGGTTAA
GCGCTTCCAATCCGAACCCGGCATTACCATTCCTCATATGGGCTGGAACCAACTGGAGTATCAACAACCTGATTTGCCCC
TATGGCGTCATTCTCCTGCTCAGCCTTGGGTCTATTTCGTGCATTCTTACTACGTTGACCCCGTTGACCCCACCGTTAAA
GCCGCCACCGTCACCCACGGCACTCAAACCATTACCGCAGCCATTGCCCGAGATAACCTAATGGCCGTTCAGTTCCACCC
CGAAAAATCTTCCACTTTCGGGTTACAAATCCTGGCCAACTTTGTTGAGCAGGTGCAGGCCACCTTGGCGACTCCCGCTG
TTTGA

Upstream 100 bases:

>100_bases
AATAGGGCTGACTCCAAATCCAGTCACCTCGCTGAAACCGAAGAGAAAGCGATAAGGTAATAAAGTAGAGATGTGGCCTA
GCTGGGAAAGGAAGGGATGA

Downstream 100 bases:

>100_bases
CCCCCATGAATTACTTAATTGCCGTCCTAGCCGACCGCATCCAAGCAGAAGCCGCCTATACCGACTTGGAAAAAGCAGGC
ATTCCCCTCACTCAAGTTTC

Product: imidazole glycerol phosphate synthase subunit HisH

Products: NA

Alternate protein names: IGP synthase glutamine amidotransferase subunit; IGP synthase subunit hisH; ImGP synthase subunit hisH; IGPS subunit hisH

Number of amino acids: Translated: 214; Mature: 213

Protein sequence:

>214_residues
MPIIAVIDYDMGNLHSACKGLQEAGTQTIVSDRPEDLVSADAVVLPGVGAFDPAMQHLRSRQLIPVIQDILASGKPFLGI
CLGLQILFEGSEEGTEAGLGIIPGTVKRFQSEPGITIPHMGWNQLEYQQPDLPLWRHSPAQPWVYFVHSYYVDPVDPTVK
AATVTHGTQTITAAIARDNLMAVQFHPEKSSTFGLQILANFVEQVQATLATPAV

Sequences:

>Translated_214_residues
MPIIAVIDYDMGNLHSACKGLQEAGTQTIVSDRPEDLVSADAVVLPGVGAFDPAMQHLRSRQLIPVIQDILASGKPFLGI
CLGLQILFEGSEEGTEAGLGIIPGTVKRFQSEPGITIPHMGWNQLEYQQPDLPLWRHSPAQPWVYFVHSYYVDPVDPTVK
AATVTHGTQTITAAIARDNLMAVQFHPEKSSTFGLQILANFVEQVQATLATPAV
>Mature_213_residues
PIIAVIDYDMGNLHSACKGLQEAGTQTIVSDRPEDLVSADAVVLPGVGAFDPAMQHLRSRQLIPVIQDILASGKPFLGIC
LGLQILFEGSEEGTEAGLGIIPGTVKRFQSEPGITIPHMGWNQLEYQQPDLPLWRHSPAQPWVYFVHSYYVDPVDPTVKA
ATVTHGTQTITAAIARDNLMAVQFHPEKSSTFGLQILANFVEQVQATLATPAV

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisH subunit provides the glutamine amidotransferase activity that produces the ammonia necessary to hisF for the synthesis of IGP and AICAR

COG id: COG0118

COG function: function code E; Glutamine amidotransferase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain

Homologues:

Organism=Escherichia coli, GI1788334, Length=201, Percent_Identity=38.3084577114428, Blast_Score=124, Evalue=6e-30,
Organism=Saccharomyces cerevisiae, GI6319725, Length=215, Percent_Identity=32.5581395348837, Blast_Score=116, Evalue=2e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HIS5_ACAM1 (B0BYP8)

Other databases:

- EMBL:   CP000828
- RefSeq:   YP_001516378.1
- ProteinModelPortal:   B0BYP8
- SMR:   B0BYP8
- GeneID:   5680863
- GenomeReviews:   CP000828_GR
- KEGG:   amr:AM1_2049
- HOGENOM:   HBG292341
- OMA:   SVRFAFE
- ProtClustDB:   PRK13141
- BioCyc:   AMAR329726:AM1_2049-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00278
- InterPro:   IPR017926
- InterPro:   IPR000991
- InterPro:   IPR010139
- InterPro:   IPR016226
- PIRSF:   PIRSF000495
- TIGRFAMs:   TIGR01855

Pfam domain/function: PF00117 GATase

EC number: 2.4.2.-

Molecular weight: Translated: 23195; Mature: 23064

Theoretical pI: Translated: 4.77; Mature: 4.77

Prosite motif: PS51273 GATASE_TYPE_1; PS00442 GATASE_TYPE_I

Important sites: ACT_SITE 81-81 ACT_SITE 186-186 ACT_SITE 188-188

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPIIAVIDYDMGNLHSACKGLQEAGTQTIVSDRPEDLVSADAVVLPGVGAFDPAMQHLRS
CCEEEEEECCCCHHHHHHHHHHHCCCCCHHCCCCHHHHCCCEEEECCCCCCCHHHHHHHC
RQLIPVIQDILASGKPFLGICLGLQILFEGSEEGTEAGLGIIPGTVKRFQSEPGITIPHM
CCHHHHHHHHHHCCCCHHHHHHHHHHEECCCCCCCCCCCCCCCHHHHHHCCCCCCCCCCC
GWNQLEYQQPDLPLWRHSPAQPWVYFVHSYYVDPVDPTVKAATVTHGTQTITAAIARDNL
CCCCCCCCCCCCCCCCCCCCCCCEEEEEHHCCCCCCCCCEEEEEECCCHHHHHHHHCCCE
MAVQFHPEKSSTFGLQILANFVEQVQATLATPAV
EEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
PIIAVIDYDMGNLHSACKGLQEAGTQTIVSDRPEDLVSADAVVLPGVGAFDPAMQHLRS
CEEEEEECCCCHHHHHHHHHHHCCCCCHHCCCCHHHHCCCEEEECCCCCCCHHHHHHHC
RQLIPVIQDILASGKPFLGICLGLQILFEGSEEGTEAGLGIIPGTVKRFQSEPGITIPHM
CCHHHHHHHHHHCCCCHHHHHHHHHHEECCCCCCCCCCCCCCCHHHHHHCCCCCCCCCCC
GWNQLEYQQPDLPLWRHSPAQPWVYFVHSYYVDPVDPTVKAATVTHGTQTITAAIARDNL
CCCCCCCCCCCCCCCCCCCCCCCEEEEEHHCCCCCCCCCEEEEEECCCHHHHHHHHCCCE
MAVQFHPEKSSTFGLQILANFVEQVQATLATPAV
EEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Glycosyltransferases; Pentosyltransferases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA