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The map label for this gene is panB

Identifier: 158335205

GI number: 158335205

Start: 2042066

End: 2042845

Strand: Direct

Name: panB

Synonym: AM1_2048

Alternate gene names: 158335205

Gene position: 2042066-2042845 (Clockwise)

Preceding gene: 158335203

Following gene: 158335206

Centisome position: 31.4

GC content: 51.79

Gene sequence:

>780_bases
ATGGCTGTGACCATTCATCAGCTACGGACATGGAAAGAACAGGGACGTCCCATCTCAGTCCTGACCGCTTGGGACTTTCC
CTTTGCAACGTTATTAGATCAAGCAGGAGTCGATGTCTTATTGGTGGGAGACTCCCTAGCCATGGTTGCCTTAGGATATC
CCACCACTCTCCCGTTGACCTTGGACGAAATGCTTTACCATGCACAAGCTGTGACTAGAGGGGTCAACCATGCCCTAGTC
ATTTGTGATCTACCCTTTCTGAGCTATCAAGAAAGTCCGCAACAGGCCTTGAGAAGTGCTGGGACCTTATTGCAACAGGC
AGGCGTAGCCGCCGTCAAACTAGAGGGAGGATATCCAGATATGGCGAAAACCGTTCACTATCTGGTTGACCGAGGAATCC
CAGTGATGGGACATGTGGGCTTAACCCCCCAGTCTGTTCATCAGTTCAGCGGCTATCGACGTCAAGGGACCTCCGATGTA
GAGGCGGATCGTATTTTACAAGAAGCGATTGCCTTGGAAGCTGCAGGCGCGTTTGCCATTGTTCTGGAGCATATTCCTGC
CCCCCTAGCCACGAAAATCACCCAAACATTGACCATTCCCACCATTGGCATCGGCGCAGGTCCAGGCTGTGATGGCCAGG
TGCTGGTTACAGCCGATATGTTGGGCCTGTCTGCATGGCAACCGCCCTTTGCCAAAGCCTATACCAATTTGCAGGAATCG
ATTACTGGAGCCGTGCAGCAATTTTGTGATGATGTCCGTCATCACCAATTTCCCCAATAG

Upstream 100 bases:

>100_bases
AGATCTTTACTTCAGACCAGCGCAAGTCAGGATACCTCTCCCTGAAACCCATCGAAGACCCTTTATTCTGGGAAAGAAGA
CACAAAGGAGCCATAAGACT

Downstream 100 bases:

>100_bases
GGCTGACTCCAAATCCAGTCACCTCGCTGAAACCGAAGAGAAAGCGATAAGGTAATAAAGTAGAGATGTGGCCTAGCTGG
GAAAGGAAGGGATGAATGCC

Product: 3-methyl-2-oxobutanoate hydroxymethyltransferase

Products: NA

Alternate protein names: Ketopantoate hydroxymethyltransferase; KPHMT

Number of amino acids: Translated: 259; Mature: 258

Protein sequence:

>259_residues
MAVTIHQLRTWKEQGRPISVLTAWDFPFATLLDQAGVDVLLVGDSLAMVALGYPTTLPLTLDEMLYHAQAVTRGVNHALV
ICDLPFLSYQESPQQALRSAGTLLQQAGVAAVKLEGGYPDMAKTVHYLVDRGIPVMGHVGLTPQSVHQFSGYRRQGTSDV
EADRILQEAIALEAAGAFAIVLEHIPAPLATKITQTLTIPTIGIGAGPGCDGQVLVTADMLGLSAWQPPFAKAYTNLQES
ITGAVQQFCDDVRHHQFPQ

Sequences:

>Translated_259_residues
MAVTIHQLRTWKEQGRPISVLTAWDFPFATLLDQAGVDVLLVGDSLAMVALGYPTTLPLTLDEMLYHAQAVTRGVNHALV
ICDLPFLSYQESPQQALRSAGTLLQQAGVAAVKLEGGYPDMAKTVHYLVDRGIPVMGHVGLTPQSVHQFSGYRRQGTSDV
EADRILQEAIALEAAGAFAIVLEHIPAPLATKITQTLTIPTIGIGAGPGCDGQVLVTADMLGLSAWQPPFAKAYTNLQES
ITGAVQQFCDDVRHHQFPQ
>Mature_258_residues
AVTIHQLRTWKEQGRPISVLTAWDFPFATLLDQAGVDVLLVGDSLAMVALGYPTTLPLTLDEMLYHAQAVTRGVNHALVI
CDLPFLSYQESPQQALRSAGTLLQQAGVAAVKLEGGYPDMAKTVHYLVDRGIPVMGHVGLTPQSVHQFSGYRRQGTSDVE
ADRILQEAIALEAAGAFAIVLEHIPAPLATKITQTLTIPTIGIGAGPGCDGQVLVTADMLGLSAWQPPFAKAYTNLQESI
TGAVQQFCDDVRHHQFPQ

Specific function: Catalyzes the reversible reaction in which hydroxymethyl group from 5,10-methylenetetrahydrofolate is tranferred onto alpha-ketoisovalerate to form ketopantoate

COG id: COG0413

COG function: function code H; Ketopantoate hydroxymethyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the panB family

Homologues:

Organism=Escherichia coli, GI1786326, Length=256, Percent_Identity=44.140625, Blast_Score=213, Evalue=8e-57,
Organism=Saccharomyces cerevisiae, GI6319653, Length=263, Percent_Identity=38.7832699619772, Blast_Score=193, Evalue=2e-50,

Paralogues:

None

Copy number: 1080 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): PANB_ACAM1 (B0BYP7)

Other databases:

- EMBL:   CP000828
- RefSeq:   YP_001516377.1
- ProteinModelPortal:   B0BYP7
- SMR:   B0BYP7
- GeneID:   5680862
- GenomeReviews:   CP000828_GR
- KEGG:   amr:AM1_2048
- HOGENOM:   HBG299908
- OMA:   YATPEQT
- BioCyc:   AMAR329726:AM1_2048-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00156
- InterPro:   IPR003700
- InterPro:   IPR015813
- Gene3D:   G3DSA:3.20.20.60
- PANTHER:   PTHR20881
- PIRSF:   PIRSF000388
- TIGRFAMs:   TIGR00222

Pfam domain/function: PF02548 Pantoate_transf; SSF51621 Pyrv/PenolPyrv_Kinase_cat

EC number: =2.1.2.11

Molecular weight: Translated: 27847; Mature: 27716

Theoretical pI: Translated: 5.46; Mature: 5.46

Prosite motif: NA

Important sites: ACT_SITE 183-183 BINDING 83-83 BINDING 113-113

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVTIHQLRTWKEQGRPISVLTAWDFPFATLLDQAGVDVLLVGDSLAMVALGYPTTLPLT
CEEEHHHHHHHHHCCCCEEEEEECCCCHHHHHHHCCCEEEEECCCEEEEEECCCCCCCHH
LDEMLYHAQAVTRGVNHALVICDLPFLSYQESPQQALRSAGTLLQQAGVAAVKLEGGYPD
HHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCEEEEECCCCCH
MAKTVHYLVDRGIPVMGHVGLTPQSVHQFSGYRRQGTSDVEADRILQEAIALEAAGAFAI
HHHHHHHHHHCCCCEEEECCCCHHHHHHHCCHHCCCCCCHHHHHHHHHHHHHHHCCHHHH
VLEHIPAPLATKITQTLTIPTIGIGAGPGCDGQVLVTADMLGLSAWQPPFAKAYTNLQES
HHHHCCCCHHHHHHHHEECCEEECCCCCCCCCCEEEEEEHHCCCCCCCHHHHHHHHHHHH
ITGAVQQFCDDVRHHQFPQ
HHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
AVTIHQLRTWKEQGRPISVLTAWDFPFATLLDQAGVDVLLVGDSLAMVALGYPTTLPLT
EEEHHHHHHHHHCCCCEEEEEECCCCHHHHHHHCCCEEEEECCCEEEEEECCCCCCCHH
LDEMLYHAQAVTRGVNHALVICDLPFLSYQESPQQALRSAGTLLQQAGVAAVKLEGGYPD
HHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCEEEEECCCCCH
MAKTVHYLVDRGIPVMGHVGLTPQSVHQFSGYRRQGTSDVEADRILQEAIALEAAGAFAI
HHHHHHHHHHCCCCEEEECCCCHHHHHHHCCHHCCCCCCHHHHHHHHHHHHHHHCCHHHH
VLEHIPAPLATKITQTLTIPTIGIGAGPGCDGQVLVTADMLGLSAWQPPFAKAYTNLQES
HHHHCCCCHHHHHHHHEECCEEECCCCCCCCCCEEEEEEHHCCCCCCCHHHHHHHHHHHH
ITGAVQQFCDDVRHHQFPQ
HHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA