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The map label for this gene is proA

Identifier: 158335171

GI number: 158335171

Start: 2002223

End: 2003518

Strand: Reverse

Name: proA

Synonym: AM1_2014

Alternate gene names: 158335171

Gene position: 2003518-2002223 (Counterclockwise)

Preceding gene: 158335172

Following gene: 158335169

Centisome position: 30.81

GC content: 51.47

Gene sequence:

>1296_bases
GTGACTGTCCTAACGAATTCTTTGACTGAGGTCGCCCAGCAAACGCGACAGGCGGCAATCCAACTGGCAGGGGCTTCTAC
AGAAACAAAAAACCAAGCCTTAGAAGCCGTCGCCCAAGCCCTAGAAGCGGCAACGCCAGAGATTTTAGAAGCGAATGTGC
AGGATTGTCAGCAGGCAAAAGCAGCAGGCATTGCTAATGCCCTCTATGCTCGGTTAAAGCTGGATGCCACCAAGCTCAAA
GGTGCGATCGCAGGGGTACGCAGTGTTGCCACTCTGGAGGATCCAGTTGGGACAGTGCAGCTGAAGCGAGAGCTAGATAC
AGATCTTGTTCTGAGTCGGGTTAGCTGTCCGTTGGGGGTACTCGGAGTCATTTTTGAAGCCCGACCCGATGCAGTGATGC
AAATTGCAGCGTTGGCCATGAAGTCTGGTAATGGCGTTTTGCTCAAAGGGGGGCAAGAAGCTCTTCATTCCTGTCAGGCA
TTAGTCAAGGCCATTCATCAAGGTCTCTCCAACACAGCGGTATCACCCAACGTAGTGGCGTTACTCACTAGCCGAGAAGA
AACCCTAGAATTGCTGAACCTCGATCAGGATGTGAACCTGATTATTCCCCGTGGTTCCAATGACTTTGTTCGGTTTGTGC
AAGAAAATACTCGTATTCCGGTGTTGGGCCATGCGGAAGGCATCTGCCATCTGTATGTAGATCAGGCAGCCAAGCTGGAC
CAAGCGGTAGAGATTGCAGTGGATTCCAAGATACAATATCCGGCGGCCTGTAATGCGGTGGAAACGATTTTGGTGCATGA
TGCGATCGCACCTCAATACCTACCCAAACTAACGGCGGCGTTACAGGATAACCACGTCAAAGTTCTAGGGGATGCCAAAA
CTCAGGCCATTGTGGATGTTGGCCCCGCAACGGAGACGGACTGGGCGACAGAATATGGGGATTTAATTGTTTCTATTAAA
GTGGTGGCGTCTCTATCCGATGCCATTGCTCATATTAATCAATACGGATCGGGACACACGGATGCCATTGCCACTGAAGA
CGATCAAGCAGCAGCTGTGTTTATGAACCAGGTGGATGCAGCCGGGGTATTCCACAACTGTTCTACTCGATTTGCCGACG
GCTTCCGCTATGGCTTTGGCGCGGAAGTGGGTATTAGCACCCAGCGGATGCCCCCCAGAGGCCCCGTCGGTTTAGAAGGA
TTAGTTACCTATAAGTATCAGCTCAAAGGCCAAGGCCAGATTGTTGCTACCTATGCGGGGTCAGATGCTCGCCCGTTTAC
CCACAAAGATCTTTAA

Upstream 100 bases:

>100_bases
ATGATTATGAGCCTCAAGAGCTGAAAATCCCTGAACCGCAAAAAATTGCCGAGTACGAACGCGAAGATTTTTAAAACCCT
GTTGTTAAGCCACTAGAACT

Downstream 100 bases:

>100_bases
TACCTCTGACATATCAGCATCATCCGTACGTTCAACCTTGTACCGATGATGCTGATATGTCAGAGGAGTGGGGTTATTGG
CGAGATGACATGGACATCAC

Product: gamma-glutamyl phosphate reductase

Products: NA

Alternate protein names: GPR; Glutamate-5-semialdehyde dehydrogenase; Glutamyl-gamma-semialdehyde dehydrogenase; GSA dehydrogenase

Number of amino acids: Translated: 431; Mature: 430

Protein sequence:

>431_residues
MTVLTNSLTEVAQQTRQAAIQLAGASTETKNQALEAVAQALEAATPEILEANVQDCQQAKAAGIANALYARLKLDATKLK
GAIAGVRSVATLEDPVGTVQLKRELDTDLVLSRVSCPLGVLGVIFEARPDAVMQIAALAMKSGNGVLLKGGQEALHSCQA
LVKAIHQGLSNTAVSPNVVALLTSREETLELLNLDQDVNLIIPRGSNDFVRFVQENTRIPVLGHAEGICHLYVDQAAKLD
QAVEIAVDSKIQYPAACNAVETILVHDAIAPQYLPKLTAALQDNHVKVLGDAKTQAIVDVGPATETDWATEYGDLIVSIK
VVASLSDAIAHINQYGSGHTDAIATEDDQAAAVFMNQVDAAGVFHNCSTRFADGFRYGFGAEVGISTQRMPPRGPVGLEG
LVTYKYQLKGQGQIVATYAGSDARPFTHKDL

Sequences:

>Translated_431_residues
MTVLTNSLTEVAQQTRQAAIQLAGASTETKNQALEAVAQALEAATPEILEANVQDCQQAKAAGIANALYARLKLDATKLK
GAIAGVRSVATLEDPVGTVQLKRELDTDLVLSRVSCPLGVLGVIFEARPDAVMQIAALAMKSGNGVLLKGGQEALHSCQA
LVKAIHQGLSNTAVSPNVVALLTSREETLELLNLDQDVNLIIPRGSNDFVRFVQENTRIPVLGHAEGICHLYVDQAAKLD
QAVEIAVDSKIQYPAACNAVETILVHDAIAPQYLPKLTAALQDNHVKVLGDAKTQAIVDVGPATETDWATEYGDLIVSIK
VVASLSDAIAHINQYGSGHTDAIATEDDQAAAVFMNQVDAAGVFHNCSTRFADGFRYGFGAEVGISTQRMPPRGPVGLEG
LVTYKYQLKGQGQIVATYAGSDARPFTHKDL
>Mature_430_residues
TVLTNSLTEVAQQTRQAAIQLAGASTETKNQALEAVAQALEAATPEILEANVQDCQQAKAAGIANALYARLKLDATKLKG
AIAGVRSVATLEDPVGTVQLKRELDTDLVLSRVSCPLGVLGVIFEARPDAVMQIAALAMKSGNGVLLKGGQEALHSCQAL
VKAIHQGLSNTAVSPNVVALLTSREETLELLNLDQDVNLIIPRGSNDFVRFVQENTRIPVLGHAEGICHLYVDQAAKLDQ
AVEIAVDSKIQYPAACNAVETILVHDAIAPQYLPKLTAALQDNHVKVLGDAKTQAIVDVGPATETDWATEYGDLIVSIKV
VASLSDAIAHINQYGSGHTDAIATEDDQAAAVFMNQVDAAGVFHNCSTRFADGFRYGFGAEVGISTQRMPPRGPVGLEGL
VTYKYQLKGQGQIVATYAGSDARPFTHKDL

Specific function: Catalyzes the NADPH dependent reduction of L-gamma- glutamyl 5-phosphate into L-glutamate 5-semialdehyde and phosphate. The product spontaneously undergoes cyclization to form 1-pyrroline-5-carboxylate

COG id: COG0014

COG function: function code E; Gamma-glutamyl phosphate reductase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gamma-glutamyl phosphate reductase family

Homologues:

Organism=Homo sapiens, GI62912457, Length=423, Percent_Identity=42.789598108747, Blast_Score=336, Evalue=3e-92,
Organism=Homo sapiens, GI21361368, Length=423, Percent_Identity=42.789598108747, Blast_Score=336, Evalue=3e-92,
Organism=Escherichia coli, GI1786438, Length=406, Percent_Identity=39.6551724137931, Blast_Score=301, Evalue=4e-83,
Organism=Caenorhabditis elegans, GI17569967, Length=429, Percent_Identity=42.6573426573427, Blast_Score=329, Evalue=2e-90,
Organism=Caenorhabditis elegans, GI17569969, Length=429, Percent_Identity=42.6573426573427, Blast_Score=329, Evalue=2e-90,
Organism=Saccharomyces cerevisiae, GI6324899, Length=448, Percent_Identity=42.1875, Blast_Score=346, Evalue=4e-96,
Organism=Drosophila melanogaster, GI21357643, Length=426, Percent_Identity=44.3661971830986, Blast_Score=351, Evalue=4e-97,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PROA_ACAM1 (B0CFL0)

Other databases:

- EMBL:   CP000828
- RefSeq:   YP_001516343.1
- ProteinModelPortal:   B0CFL0
- SMR:   B0CFL0
- GeneID:   5680828
- GenomeReviews:   CP000828_GR
- KEGG:   amr:AM1_2014
- HOGENOM:   HBG318080
- OMA:   QYPAACN
- ProtClustDB:   PRK00197
- BioCyc:   AMAR329726:AM1_2014-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00412
- InterPro:   IPR016161
- InterPro:   IPR016163
- InterPro:   IPR016162
- InterPro:   IPR015590
- InterPro:   IPR000965
- InterPro:   IPR020593
- InterPro:   IPR012134
- Gene3D:   G3DSA:3.40.309.10
- Gene3D:   G3DSA:3.40.605.10
- PIRSF:   PIRSF000151
- TIGRFAMs:   TIGR00407

Pfam domain/function: PF00171 Aldedh; SSF53720 Aldehyde_DH/Histidinol_DH

EC number: =1.2.1.41

Molecular weight: Translated: 45666; Mature: 45535

Theoretical pI: Translated: 4.92; Mature: 4.92

Prosite motif: PS01223 PROA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVLTNSLTEVAQQTRQAAIQLAGASTETKNQALEAVAQALEAATPEILEANVQDCQQAK
CCEEHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCHHHHHCCHHHHHHHH
AAGIANALYARLKLDATKLKGAIAGVRSVATLEDPVGTVQLKRELDTDLVLSRVSCPLGV
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCHHHHHHHHCCCHHH
LGVIFEARPDAVMQIAALAMKSGNGVLLKGGQEALHSCQALVKAIHQGLSNTAVSPNVVA
HHHHHHCCCHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHHHHCCCCCCCCCCEEE
LLTSREETLELLNLDQDVNLIIPRGSNDFVRFVQENTRIPVLGHAEGICHLYVDQAAKLD
EEECCHHHHHHHCCCCCCEEEEECCCCHHHHHHHHCCCCEEEECCCCEEEEEEHHHHHHH
QAVEIAVDSKIQYPAACNAVETILVHDAIAPQYLPKLTAALQDNHVKVLGDAKTQAIVDV
HHHEEEECCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCEEEEECCCCEEEEEC
GPATETDWATEYGDLIVSIKVVASLSDAIAHINQYGSGHTDAIATEDDQAAAVFMNQVDA
CCCCCCCCHHHHHHHEEHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHH
AGVFHNCSTRFADGFRYGFGAEVGISTQRMPPRGPVGLEGLVTYKYQLKGQGQIVATYAG
HHHHHCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEEECCCCEEEEEECC
SDARPFTHKDL
CCCCCCCCCCC
>Mature Secondary Structure 
TVLTNSLTEVAQQTRQAAIQLAGASTETKNQALEAVAQALEAATPEILEANVQDCQQAK
CEEHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCHHHHHCCHHHHHHHH
AAGIANALYARLKLDATKLKGAIAGVRSVATLEDPVGTVQLKRELDTDLVLSRVSCPLGV
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCHHHHHHHHCCCHHH
LGVIFEARPDAVMQIAALAMKSGNGVLLKGGQEALHSCQALVKAIHQGLSNTAVSPNVVA
HHHHHHCCCHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHHHHCCCCCCCCCCEEE
LLTSREETLELLNLDQDVNLIIPRGSNDFVRFVQENTRIPVLGHAEGICHLYVDQAAKLD
EEECCHHHHHHHCCCCCCEEEEECCCCHHHHHHHHCCCCEEEECCCCEEEEEEHHHHHHH
QAVEIAVDSKIQYPAACNAVETILVHDAIAPQYLPKLTAALQDNHVKVLGDAKTQAIVDV
HHHEEEECCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCEEEEECCCCEEEEEC
GPATETDWATEYGDLIVSIKVVASLSDAIAHINQYGSGHTDAIATEDDQAAAVFMNQVDA
CCCCCCCCHHHHHHHEEHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHH
AGVFHNCSTRFADGFRYGFGAEVGISTQRMPPRGPVGLEGLVTYKYQLKGQGQIVATYAG
HHHHHCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEEECCCCEEEEEECC
SDARPFTHKDL
CCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA