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The map label for this gene is ttcA

Identifier: 158335112

GI number: 158335112

Start: 1947980

End: 1948831

Strand: Direct

Name: ttcA

Synonym: AM1_1954

Alternate gene names: 158335112

Gene position: 1947980-1948831 (Clockwise)

Preceding gene: 158335109

Following gene: 158335114

Centisome position: 29.95

GC content: 49.3

Gene sequence:

>852_bases
TTGTCCAATCTGTTTATGACCGCCACTCAACTCCACCCCTCCAAACAACTGAAAAAGCTACAAGCTCGCCTCAGAGGGCT
AGTGGGCAAGGCCATTCGTGACTACAACCTGATCGAAGCTGGCGATCGGATTATGGTGTGTTTGTCCGGTGGCAAAGACT
CCTACACCCTGCTGGATATTTTGTTGCATCTACAGCGGGCTGCCCCCATTGATTTTGAAATCTTGGCCGTCAATCTCGAT
CAAAAACAACCCAATTTTCCAGAACAGGTTTTACCCAACTATCTAAACCAACTCCAAGTCCCTTACCGCATCGTCGAAGA
AGATACCTACAGTACGGTCAAACGAGTCATCCCAGAAGGCAAAACCATGTGTGGCCTTTGTTCTCGATTACGAAGAGGGA
TTCTCTATCGAGTTGCTCAAGAAGAACAGGCCACCAAAATTGCCCTCGGTCACCATCGAGAAGACATTATCGAAACCCTC
TTTCTGAATCTGTTCCATGCTGGCAAGCTAGAAGCCATGCCCCCCAAGCTATTGAGCGACAATCGCCAGCACATGGTGAT
CCGTCCCCTCGCTTACTGCCCCGAAGCCGATATCCAACGCTATGCCGATTTACGGCAGTTTCCGATTATTCCCTGCACCC
TATGTGGATCTCAAGAGACCTTACAACGGGTCCAAGTCAAACAAATGCTGCAAGCCTGGGAGCAAGAAACACCGGGGCGA
TTAGAAACGATCTTTCGCAGTCTACAAAATATCGAATTGTCCCAGCTTGCTGATCCACAGCTGTTTGATTTCGCCCACTT
GGCGATTGCTGCAACACCTTATGCAGATTCACCCGATAGGTCGGGGCTTTAA

Upstream 100 bases:

>100_bases
CCCGATTTAGGAATGATACTGCCATCTAGATACAACCCCCTATCCTTCAGCATTTGCTCCCTCTGAGTGAACTAAATATT
CCCCAGTATTGATTGCTCTT

Downstream 100 bases:

>100_bases
CGAATATCGAGAATACCGTCAATCCCTTCTAAACTCAACATATCCAGGCGCTCATCGGCTTCTCCACGTTTTTCATAAGC
CCCTACTTGTAAGACCGATC

Product: C32 tRNA thiolase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 283; Mature: 282

Protein sequence:

>283_residues
MSNLFMTATQLHPSKQLKKLQARLRGLVGKAIRDYNLIEAGDRIMVCLSGGKDSYTLLDILLHLQRAAPIDFEILAVNLD
QKQPNFPEQVLPNYLNQLQVPYRIVEEDTYSTVKRVIPEGKTMCGLCSRLRRGILYRVAQEEQATKIALGHHREDIIETL
FLNLFHAGKLEAMPPKLLSDNRQHMVIRPLAYCPEADIQRYADLRQFPIIPCTLCGSQETLQRVQVKQMLQAWEQETPGR
LETIFRSLQNIELSQLADPQLFDFAHLAIAATPYADSPDRSGL

Sequences:

>Translated_283_residues
MSNLFMTATQLHPSKQLKKLQARLRGLVGKAIRDYNLIEAGDRIMVCLSGGKDSYTLLDILLHLQRAAPIDFEILAVNLD
QKQPNFPEQVLPNYLNQLQVPYRIVEEDTYSTVKRVIPEGKTMCGLCSRLRRGILYRVAQEEQATKIALGHHREDIIETL
FLNLFHAGKLEAMPPKLLSDNRQHMVIRPLAYCPEADIQRYADLRQFPIIPCTLCGSQETLQRVQVKQMLQAWEQETPGR
LETIFRSLQNIELSQLADPQLFDFAHLAIAATPYADSPDRSGL
>Mature_282_residues
SNLFMTATQLHPSKQLKKLQARLRGLVGKAIRDYNLIEAGDRIMVCLSGGKDSYTLLDILLHLQRAAPIDFEILAVNLDQ
KQPNFPEQVLPNYLNQLQVPYRIVEEDTYSTVKRVIPEGKTMCGLCSRLRRGILYRVAQEEQATKIALGHHREDIIETLF
LNLFHAGKLEAMPPKLLSDNRQHMVIRPLAYCPEADIQRYADLRQFPIIPCTLCGSQETLQRVQVKQMLQAWEQETPGRL
ETIFRSLQNIELSQLADPQLFDFAHLAIAATPYADSPDRSGL

Specific function: Required for the thiolation of cytidine in position 32 of tRNA, to form 2-thiocytidine (s(2)C32)

COG id: COG0037

COG function: function code D; Predicted ATPase of the PP-loop superfamily implicated in cell cycle control

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TtcA family

Homologues:

Organism=Escherichia coli, GI1787606, Length=253, Percent_Identity=64.8221343873518, Blast_Score=354, Evalue=5e-99,
Organism=Caenorhabditis elegans, GI193211090, Length=248, Percent_Identity=31.8548387096774, Blast_Score=122, Evalue=3e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TTCA_ACAM1 (B0CEK4)

Other databases:

- EMBL:   CP000828
- RefSeq:   YP_001516284.1
- ProteinModelPortal:   B0CEK4
- GeneID:   5680768
- GenomeReviews:   CP000828_GR
- KEGG:   amr:AM1_1954
- HOGENOM:   HBG347038
- OMA:   EHILPEY
- ProtClustDB:   PRK10696
- BioCyc:   AMAR329726:AM1_1954-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01850
- InterPro:   IPR012089
- InterPro:   IPR014729
- InterPro:   IPR011063
- Gene3D:   G3DSA:3.40.50.620
- PIRSF:   PIRSF004976

Pfam domain/function: PF01171 ATP_bind_3

EC number: NA

Molecular weight: Translated: 32234; Mature: 32103

Theoretical pI: Translated: 7.50; Mature: 7.50

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNLFMTATQLHPSKQLKKLQARLRGLVGKAIRDYNLIEAGDRIMVCLSGGKDSYTLLDI
CCCCEEEHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCEECCCEEEEEEECCCCHHHHHHH
LLHLQRAAPIDFEILAVNLDQKQPNFPEQVLPNYLNQLQVPYRIVEEDTYSTVKRVIPEG
HHHHHHCCCCCEEEEEEEECCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCC
KTMCGLCSRLRRGILYRVAQEEQATKIALGHHREDIIETLFLNLFHAGKLEAMPPKLLSD
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCHHHCC
NRQHMVIRPLAYCPEADIQRYADLRQFPIIPCTLCGSQETLQRVQVKQMLQAWEQETPGR
CCCEEEEEHHHHCCCHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHHHHHCCCCHH
LETIFRSLQNIELSQLADPQLFDFAHLAIAATPYADSPDRSGL
HHHHHHHHHCCCHHHCCCCHHHHHHHHHHEECCCCCCCCCCCC
>Mature Secondary Structure 
SNLFMTATQLHPSKQLKKLQARLRGLVGKAIRDYNLIEAGDRIMVCLSGGKDSYTLLDI
CCCEEEHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCEECCCEEEEEEECCCCHHHHHHH
LLHLQRAAPIDFEILAVNLDQKQPNFPEQVLPNYLNQLQVPYRIVEEDTYSTVKRVIPEG
HHHHHHCCCCCEEEEEEEECCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCC
KTMCGLCSRLRRGILYRVAQEEQATKIALGHHREDIIETLFLNLFHAGKLEAMPPKLLSD
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCHHHCC
NRQHMVIRPLAYCPEADIQRYADLRQFPIIPCTLCGSQETLQRVQVKQMLQAWEQETPGR
CCCEEEEEHHHHCCCHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHHHHHCCCCHH
LETIFRSLQNIELSQLADPQLFDFAHLAIAATPYADSPDRSGL
HHHHHHHHHCCCHHHCCCCHHHHHHHHHHEECCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA