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The map label for this gene is mdtE [H]

Identifier: 158334929

GI number: 158334929

Start: 1754943

End: 1756157

Strand: Direct

Name: mdtE [H]

Synonym: AM1_1766

Alternate gene names: 158334929

Gene position: 1754943-1756157 (Clockwise)

Preceding gene: 158334928

Following gene: 158334930

Centisome position: 26.98

GC content: 54.73

Gene sequence:

>1215_bases
GTGGTCTGGCAATTTTGGCCCAAAGGGGCCAAAACCACGGGTCAACAGGGTCCGCCGCCGACTTTGGTCAAAGTTCAGAC
CGTCCAAACCAGTCCAGTCAAGCAAACCTCTGCCTTCCTAGGCACCTTAGAAGCCAAGCAGGGCGTGGTGCTACGCCCCG
AAACAGAAGGACGAGTCACTCAGATTTTTGTCTCATCGGGGTCGAGGGTGTCCCCTGGCCAGCCTATTGTTCAACTCAGT
CCCGAGAAATCTCAAGCAGACTTCGGCGCAGCGGTGGCCAATATCAATGTAGCCACCGCCGCCAGCAGCAATGCCCAAGC
CCAACTTCGAGCCGTCCAAGCCGATCGTGCCAGGGCAGTTGCTGAGCTGGAATTGCAAAATACAGAGTTTCAGCGGACCC
AGACCTTGGTCAAACAAGGGGCACTGGCCAAGCAAGTATTGGATCAAGTCCGCCGCGACCGCGCCTCTGCCCAAGCGGCT
CTCAGAGCAGTAGATGAGCAAATTCGTGCGGCTAAAGCCAACTTAACCCAATCTAGAGCCAGCCTCAATCAAGCCAAAGC
CAGCGCCTTATCGGCTAGAGAAGATGTACAAGATACGCGCATCACAGCCCCCATTGCGGGGATGGTGGGGGATATTCCCA
TTAAGTTAGGTTCCTTTGTACAGGCCGGTGACACCCTTACCACCATTATTCAAAATCAAACCCTAGAGATCAATCTCAAT
ATTCCGATTGAGAAGCGCGAGCAGCTCGCAGTGGGATTACCAGTCGAACTCAGCCAAGCCCAATCCCAAGACATCCTGGC
CCAGGGACGTATCAGTTTCGTCTCACCCACGGTCAATAACGAGAGTCAATCCGTCTTAGCCAAGGCCACCTTCGCCAACC
CCAAAGGGACATTACGGGATGGTCAACGGGTCGAAGCTAACGTGGTTTGGCAAACCCGTCCGGGGGTATTAGTGCCCACC
ACCGCTGTGACACGATTGGGAGGTAAAGCCTTTGTCTTTATTGCTGATCAGCCACAACAACCAGACGGTCAAGTACCTGG
GATGGTGGCTCGCCAGCAACCCGTTGAGCTAGGCAGCATCCAGGGCAACGACTACCAAGTGTTGGAGGGTATCCAAGCCG
GTGAAACCATCGTTATTTCTGGCCTACAAACCATCAGGGATGGGGCCCAACTGCGCCTTGATGACAACAAATCCAGCGGC
AAACCACCCCAGTAA

Upstream 100 bases:

>100_bases
AGTCGCGGAACGATCGTTCAACTCCACCATCAAATCCTTGAAACAACGTAGCAGTCGGAATTGGATCATTGCCGGTGTGG
CCCTTATGGGGACAGGGCTG

Downstream 100 bases:

>100_bases
GCTGCCTTAGCATTTGCACTGTACTTCACTGATTCGATATTCTCTCCCATTTAGCCTCTCTCCTTGGCTGTGCCTGCTGA
TTCCTAGCCTCCCCCCTCGC

Product: RND family efflux transporter MFP subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 404; Mature: 404

Protein sequence:

>404_residues
MVWQFWPKGAKTTGQQGPPPTLVKVQTVQTSPVKQTSAFLGTLEAKQGVVLRPETEGRVTQIFVSSGSRVSPGQPIVQLS
PEKSQADFGAAVANINVATAASSNAQAQLRAVQADRARAVAELELQNTEFQRTQTLVKQGALAKQVLDQVRRDRASAQAA
LRAVDEQIRAAKANLTQSRASLNQAKASALSAREDVQDTRITAPIAGMVGDIPIKLGSFVQAGDTLTTIIQNQTLEINLN
IPIEKREQLAVGLPVELSQAQSQDILAQGRISFVSPTVNNESQSVLAKATFANPKGTLRDGQRVEANVVWQTRPGVLVPT
TAVTRLGGKAFVFIADQPQQPDGQVPGMVARQQPVELGSIQGNDYQVLEGIQAGETIVISGLQTIRDGAQLRLDDNKSSG
KPPQ

Sequences:

>Translated_404_residues
MVWQFWPKGAKTTGQQGPPPTLVKVQTVQTSPVKQTSAFLGTLEAKQGVVLRPETEGRVTQIFVSSGSRVSPGQPIVQLS
PEKSQADFGAAVANINVATAASSNAQAQLRAVQADRARAVAELELQNTEFQRTQTLVKQGALAKQVLDQVRRDRASAQAA
LRAVDEQIRAAKANLTQSRASLNQAKASALSAREDVQDTRITAPIAGMVGDIPIKLGSFVQAGDTLTTIIQNQTLEINLN
IPIEKREQLAVGLPVELSQAQSQDILAQGRISFVSPTVNNESQSVLAKATFANPKGTLRDGQRVEANVVWQTRPGVLVPT
TAVTRLGGKAFVFIADQPQQPDGQVPGMVARQQPVELGSIQGNDYQVLEGIQAGETIVISGLQTIRDGAQLRLDDNKSSG
KPPQ
>Mature_404_residues
MVWQFWPKGAKTTGQQGPPPTLVKVQTVQTSPVKQTSAFLGTLEAKQGVVLRPETEGRVTQIFVSSGSRVSPGQPIVQLS
PEKSQADFGAAVANINVATAASSNAQAQLRAVQADRARAVAELELQNTEFQRTQTLVKQGALAKQVLDQVRRDRASAQAA
LRAVDEQIRAAKANLTQSRASLNQAKASALSAREDVQDTRITAPIAGMVGDIPIKLGSFVQAGDTLTTIIQNQTLEINLN
IPIEKREQLAVGLPVELSQAQSQDILAQGRISFVSPTVNNESQSVLAKATFANPKGTLRDGQRVEANVVWQTRPGVLVPT
TAVTRLGGKAFVFIADQPQQPDGQVPGMVARQQPVELGSIQGNDYQVLEGIQAGETIVISGLQTIRDGAQLRLDDNKSSG
KPPQ

Specific function: Part of a multidrug resistance efflux system that confers resistance to compounds such as rhodamine 6G, erythromycin, doxorubicin, ethidium bromide, TPP, SDS, deoxycholate, crystal violet and benzalkonium [H]

COG id: COG0845

COG function: function code M; Membrane-fusion protein

Gene ontology:

Cell location: Cell inner membrane; Lipid-anchor (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI1789929, Length=358, Percent_Identity=26.2569832402235, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1786668, Length=401, Percent_Identity=27.1820448877805, Blast_Score=82, Evalue=9e-17,
Organism=Escherichia coli, GI1789665, Length=386, Percent_Identity=24.8704663212435, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI87082036, Length=376, Percent_Identity=23.6702127659574, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI87081796, Length=378, Percent_Identity=24.6031746031746, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 43092; Mature: 43092

Theoretical pI: Translated: 10.32; Mature: 10.32

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
0.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
0.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVWQFWPKGAKTTGQQGPPPTLVKVQTVQTSPVKQTSAFLGTLEAKQGVVLRPETEGRVT
CCEEECCCCCCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHCCCCEEECCCCCCCEE
QIFVSSGSRVSPGQPIVQLSPEKSQADFGAAVANINVATAASSNAQAQLRAVQADRARAV
EEEEECCCCCCCCCCEEEECCCCCCCCHHHEEEEEEEEECCCCCCHHHHHHHHHHHHHHE
AELELQNTEFQRTQTLVKQGALAKQVLDQVRRDRASAQAALRAVDEQIRAAKANLTQSRA
EEEEECCCHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SLNQAKASALSAREDVQDTRITAPIAGMVGDIPIKLGSFVQAGDTLTTIIQNQTLEINLN
HHHHHHHHHHHHHHHHHHHEECCCHHHHHCCCCEECCCHHHCCCHHEEEECCCEEEEEEE
IPIEKREQLAVGLPVELSQAQSQDILAQGRISFVSPTVNNESQSVLAKATFANPKGTLRD
CCCCCCCCEEECCCCCHHHCCCHHHHHCCCEEEECCCCCCCCHHEEEEEEECCCCCCCCC
GQRVEANVVWQTRPGVLVPTTAVTRLGGKAFVFIADQPQQPDGQVPGMVARQQPVELGSI
CCEEEEEEEEEECCCEEECHHHHHHCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCC
QGNDYQVLEGIQAGETIVISGLQTIRDGAQLRLDDNKSSGKPPQ
CCCCHHHHHCCCCCCEEEEECHHHHCCCCEEEECCCCCCCCCCC
>Mature Secondary Structure
MVWQFWPKGAKTTGQQGPPPTLVKVQTVQTSPVKQTSAFLGTLEAKQGVVLRPETEGRVT
CCEEECCCCCCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHCCCCEEECCCCCCCEE
QIFVSSGSRVSPGQPIVQLSPEKSQADFGAAVANINVATAASSNAQAQLRAVQADRARAV
EEEEECCCCCCCCCCEEEECCCCCCCCHHHEEEEEEEEECCCCCCHHHHHHHHHHHHHHE
AELELQNTEFQRTQTLVKQGALAKQVLDQVRRDRASAQAALRAVDEQIRAAKANLTQSRA
EEEEECCCHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SLNQAKASALSAREDVQDTRITAPIAGMVGDIPIKLGSFVQAGDTLTTIIQNQTLEINLN
HHHHHHHHHHHHHHHHHHHEECCCHHHHHCCCCEECCCHHHCCCHHEEEECCCEEEEEEE
IPIEKREQLAVGLPVELSQAQSQDILAQGRISFVSPTVNNESQSVLAKATFANPKGTLRD
CCCCCCCCEEECCCCCHHHCCCHHHHHCCCEEEECCCCCCCCHHEEEEEEECCCCCCCCC
GQRVEANVVWQTRPGVLVPTTAVTRLGGKAFVFIADQPQQPDGQVPGMVARQQPVELGSI
CCEEEEEEEEEECCCEEECHHHHHHCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCC
QGNDYQVLEGIQAGETIVISGLQTIRDGAQLRLDDNKSSGKPPQ
CCCCHHHHHCCCCCCEEEEECHHHHCCCCEEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]