Click here to switch to the map view.

The map label for this gene is ksgA

Identifier: 158334916

GI number: 158334916

Start: 1741078

End: 1741887

Strand: Reverse

Name: ksgA

Synonym: AM1_1753

Alternate gene names: 158334916

Gene position: 1741887-1741078 (Counterclockwise)

Preceding gene: 158334932

Following gene: 158334915

Centisome position: 26.78

GC content: 50.49

Gene sequence:

>810_bases
ATGCCCAGGCCCCGTAAACGTTTTGCTCAGCATTGGCTCAAAAGTCAGGCTGTGCTTCGGCAGATTATTGCTGCAGCCAA
GATACAAGGATGCGATCGCATCCTTGAAATCGGTCCGGGCAGAGGGGTCTTAACCCGCGAACTGCTGGCCCAAGCTCAGT
CCGTGGTTTCCGTTGAGCTAGATCGAGACCTATGCAAATCCCTGCGTCACACCTTTAATGACCAGGAAAATTTCACATTA
CTGGAATTGGACTTTCTGAATCTGGATGTGGCGGCAGAACTCACAGAGCCGCTCCCGAATAAAGTCGTTGCCAATATTCC
CTACAACATTACGAGTCCGATTCTAAGTAAGCTGCTGGGCCGTATTGATGCACCAGCGCAGCCCGTTTATGAAACGGTGG
TATTGCTGATCCAAAAAGAGGTGGCGGATCGGTTGGTGGCCGAACCGGGTTCAAAGATATTTAATGGATTGTCGGTGCGA
TCGCAATATCTAGCGGACTGCGAATTAATATGTCCAGTCCCCGCCTCTGCCTTTAAGCCTGCCCCTAAAGTGGAGTCGGC
TGTAGTGCGACTAACCCCTCGCCCCTATCCCCAACCTGTGCAAAATCCGCAATGGCTTTCAACCTTACTAAAGGTAGGAT
TTAGCAGTCGTCGCAAGATGCTGCGCAATAATCTGAAATCACTAGTGGATCGGGACCAGTTATCAGAGTGCTTGAATACC
CTCAATATCAGTCTCCAGGCTCGTGCAGAAGACCTGAGTGTGACCCAGTGGATTGCCTTAAGTGATTCTGTGCATCCAAG
CCAGGTATAG

Upstream 100 bases:

>100_bases
GCCTTGGTGGTTTGGGACAATTATAATGAAAAGCTTAAGAATGTGCGGAGCGAAAGCCTGGATCTCAGGCTAATATTGAT
GGTATTTCGGTAAATCTACA

Downstream 100 bases:

>100_bases
ACGAGGTAGAGTGTTGATTGCACTGGTTCATCCGGCCTCTAACTTATGATGCACGCTTATACCCTGAAGGCTCCCGCCAA
GATCAATCTATTGTTGGAAA

Product: dimethyladenosine transferase

Products: NA

Alternate protein names: 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferase; 16S rRNA dimethyladenosine transferase; 16S rRNA dimethylase; S-adenosylmethionine-6-N', N'-adenosyl(rRNA) dimethyltransferase

Number of amino acids: Translated: 269; Mature: 268

Protein sequence:

>269_residues
MPRPRKRFAQHWLKSQAVLRQIIAAAKIQGCDRILEIGPGRGVLTRELLAQAQSVVSVELDRDLCKSLRHTFNDQENFTL
LELDFLNLDVAAELTEPLPNKVVANIPYNITSPILSKLLGRIDAPAQPVYETVVLLIQKEVADRLVAEPGSKIFNGLSVR
SQYLADCELICPVPASAFKPAPKVESAVVRLTPRPYPQPVQNPQWLSTLLKVGFSSRRKMLRNNLKSLVDRDQLSECLNT
LNISLQARAEDLSVTQWIALSDSVHPSQV

Sequences:

>Translated_269_residues
MPRPRKRFAQHWLKSQAVLRQIIAAAKIQGCDRILEIGPGRGVLTRELLAQAQSVVSVELDRDLCKSLRHTFNDQENFTL
LELDFLNLDVAAELTEPLPNKVVANIPYNITSPILSKLLGRIDAPAQPVYETVVLLIQKEVADRLVAEPGSKIFNGLSVR
SQYLADCELICPVPASAFKPAPKVESAVVRLTPRPYPQPVQNPQWLSTLLKVGFSSRRKMLRNNLKSLVDRDQLSECLNT
LNISLQARAEDLSVTQWIALSDSVHPSQV
>Mature_268_residues
PRPRKRFAQHWLKSQAVLRQIIAAAKIQGCDRILEIGPGRGVLTRELLAQAQSVVSVELDRDLCKSLRHTFNDQENFTLL
ELDFLNLDVAAELTEPLPNKVVANIPYNITSPILSKLLGRIDAPAQPVYETVVLLIQKEVADRLVAEPGSKIFNGLSVRS
QYLADCELICPVPASAFKPAPKVESAVVRLTPRPYPQPVQNPQWLSTLLKVGFSSRRKMLRNNLKSLVDRDQLSECLNTL
NISLQARAEDLSVTQWIALSDSVHPSQV

Specific function: Specifically dimethylates two adjacent adenosines (A1518 and A1519) in the loop of a conserved hairpin near the 3'-end of 16S rRNA in the 30S particle. May play a critical role in biogenesis of 30S subunits

COG id: COG0030

COG function: function code J; Dimethyladenosine transferase (rRNA methylation)

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family. RsmA subfamily

Homologues:

Organism=Homo sapiens, GI7657198, Length=258, Percent_Identity=33.7209302325581, Blast_Score=126, Evalue=2e-29,
Organism=Homo sapiens, GI156415992, Length=275, Percent_Identity=28.7272727272727, Blast_Score=95, Evalue=9e-20,
Organism=Escherichia coli, GI1786236, Length=262, Percent_Identity=37.4045801526718, Blast_Score=155, Evalue=2e-39,
Organism=Caenorhabditis elegans, GI25146882, Length=213, Percent_Identity=36.150234741784, Blast_Score=126, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI25141369, Length=259, Percent_Identity=29.3436293436293, Blast_Score=87, Evalue=7e-18,
Organism=Saccharomyces cerevisiae, GI6324989, Length=239, Percent_Identity=33.8912133891213, Blast_Score=110, Evalue=3e-25,
Organism=Drosophila melanogaster, GI21358017, Length=224, Percent_Identity=37.0535714285714, Blast_Score=136, Evalue=1e-32,
Organism=Drosophila melanogaster, GI21357273, Length=274, Percent_Identity=26.2773722627737, Blast_Score=75, Evalue=4e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RSMA_ACAM1 (B0CC89)

Other databases:

- EMBL:   CP000828
- RefSeq:   YP_001516088.1
- ProteinModelPortal:   B0CC89
- SMR:   B0CC89
- GeneID:   5680569
- GenomeReviews:   CP000828_GR
- KEGG:   amr:AM1_1753
- HOGENOM:   HBG319664
- OMA:   TPIIMKL
- ProtClustDB:   PRK00274
- BioCyc:   AMAR329726:AM1_1753-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00607
- InterPro:   IPR023165
- InterPro:   IPR020596
- InterPro:   IPR001737
- InterPro:   IPR020598
- InterPro:   IPR011530
- Gene3D:   G3DSA:1.10.8.100
- PANTHER:   PTHR11727
- SMART:   SM00650
- TIGRFAMs:   TIGR00755

Pfam domain/function: PF00398 RrnaAD

EC number: =2.1.1.182

Molecular weight: Translated: 30000; Mature: 29869

Theoretical pI: Translated: 9.00; Mature: 9.00

Prosite motif: PS01131 RRNA_A_DIMETH

Important sites: BINDING 11-11 BINDING 13-13 BINDING 38-38 BINDING 59-59 BINDING 84-84 BINDING 105-105

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPRPRKRFAQHWLKSQAVLRQIIAAAKIQGCDRILEIGPGRGVLTRELLAQAQSVVSVEL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHH
DRDLCKSLRHTFNDQENFTLLELDFLNLDVAAELTEPLPNKVVANIPYNITSPILSKLLG
HHHHHHHHHHHCCCCCCEEEEEEEECCHHHHHHHHCCCCCCEEECCCCCCHHHHHHHHHH
RIDAPAQPVYETVVLLIQKEVADRLVAEPGSKIFNGLSVRSQYLADCELICPVPASAFKP
HCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCEECCCCHHHCCC
APKVESAVVRLTPRPYPQPVQNPQWLSTLLKVGFSSRRKMLRNNLKSLVDRDQLSECLNT
CCCHHHHEEEECCCCCCCCCCCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHH
LNISLQARAEDLSVTQWIALSDSVHPSQV
HCCHHEECCCHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
PRPRKRFAQHWLKSQAVLRQIIAAAKIQGCDRILEIGPGRGVLTRELLAQAQSVVSVEL
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHH
DRDLCKSLRHTFNDQENFTLLELDFLNLDVAAELTEPLPNKVVANIPYNITSPILSKLLG
HHHHHHHHHHHCCCCCCEEEEEEEECCHHHHHHHHCCCCCCEEECCCCCCHHHHHHHHHH
RIDAPAQPVYETVVLLIQKEVADRLVAEPGSKIFNGLSVRSQYLADCELICPVPASAFKP
HCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCEECCCCHHHCCC
APKVESAVVRLTPRPYPQPVQNPQWLSTLLKVGFSSRRKMLRNNLKSLVDRDQLSECLNT
CCCHHHHEEEECCCCCCCCCCCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHH
LNISLQARAEDLSVTQWIALSDSVHPSQV
HCCHHEECCCHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA