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The map label for this gene is ispE

Identifier: 158334915

GI number: 158334915

Start: 1740097

End: 1741029

Strand: Reverse

Name: ispE

Synonym: AM1_1752

Alternate gene names: 158334915

Gene position: 1741029-1740097 (Counterclockwise)

Preceding gene: 158334916

Following gene: 158334914

Centisome position: 26.77

GC content: 50.8

Gene sequence:

>933_bases
ATGCACGCTTATACCCTGAAGGCTCCCGCCAAGATCAATCTATTGTTGGAAATTATTGGTGATCGACCGGATGGGTTCCA
TGAATTGGTGATGGTGATGCAGAGCGTTAACTTGGCGGATATCGTTGAGTTACAGCCCCTGAGTGGCGATCGGATTGAGC
TGTTTTGCAATCATCCTCAAGTGCCTTGCGACCAGTCGAACTTGGCCCATCGGGCCGCGAGGCTGATGCAGGAACAGTTC
CCAGGCAAAGGCGGTGTCGCCATTACGATTGATAAAAAAATTCCCATTGGTGCGGGGCTGGCGGGAGGGTCTGCAGATGC
GGCAGCCGTGTTAGTGGGGTTGGATCTGATGTGGGGGTTGGGATTAACCCAAGCTGAACTCCATACCTATGCGGCCAAGT
TGGGGTCTGATATTCCCTTTTGTGTGTCGGGGGGAACGGCCTTGGCCTTAGGGCGAGGGGAAGAACTGACGCCACTTCCT
GACTTGGATCAGCTCTATGCAGTGTTGGCCAAGTATCGAAGTTTGTCCGTTGCCACGCCTTGGGCCTATAAGACCTATCG
GCAACAATTTAGTGAGTCCTATGCTCGTGATCCAGAAGCGCTAGAAAAGCGGCGACGTGAAGGACATTCGGTGGAACTGT
TGGCCGCGATTTCCCATCGCGATTCCCAAAAAGTGCCTCAATATCTATATAACGATTTGGAGAAAGTGGTTCTGCCTGAA
CATCCTCAGGTGCAGGAAATTCGTGACTTATTCCAATCTTTTGGAGCCTTGGGAACGATGATGTCAGGGTCAGGACCAAC
GGTGTTTGGGTTAGCGGATTCGCGTTCCAAGGCCGAGTTAATGGCGACCCAGTTACAAACGGCTCTTCCAGATCCAGATT
TAGAAATTCTGGTAACTAAATTTTGTAGTAGTGGCATTCAATTGTTGTCTTAG

Upstream 100 bases:

>100_bases
TGTGACCCAGTGGATTGCCTTAAGTGATTCTGTGCATCCAAGCCAGGTATAGACGAGGTAGAGTGTTGATTGCACTGGTT
CATCCGGCCTCTAACTTATG

Downstream 100 bases:

>100_bases
TAAAAAGGGCATTCAACCCGAAAGAATTGAGTTCGCTGGTTTGCTAAGAGGATTATTCGTGCTGCATGTCGGTCTATTAA
CGGTTGAGCAGCGATGAACC

Product: 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase

Products: NA

Alternate protein names: CMK; 4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol kinase

Number of amino acids: Translated: 310; Mature: 310

Protein sequence:

>310_residues
MHAYTLKAPAKINLLLEIIGDRPDGFHELVMVMQSVNLADIVELQPLSGDRIELFCNHPQVPCDQSNLAHRAARLMQEQF
PGKGGVAITIDKKIPIGAGLAGGSADAAAVLVGLDLMWGLGLTQAELHTYAAKLGSDIPFCVSGGTALALGRGEELTPLP
DLDQLYAVLAKYRSLSVATPWAYKTYRQQFSESYARDPEALEKRRREGHSVELLAAISHRDSQKVPQYLYNDLEKVVLPE
HPQVQEIRDLFQSFGALGTMMSGSGPTVFGLADSRSKAELMATQLQTALPDPDLEILVTKFCSSGIQLLS

Sequences:

>Translated_310_residues
MHAYTLKAPAKINLLLEIIGDRPDGFHELVMVMQSVNLADIVELQPLSGDRIELFCNHPQVPCDQSNLAHRAARLMQEQF
PGKGGVAITIDKKIPIGAGLAGGSADAAAVLVGLDLMWGLGLTQAELHTYAAKLGSDIPFCVSGGTALALGRGEELTPLP
DLDQLYAVLAKYRSLSVATPWAYKTYRQQFSESYARDPEALEKRRREGHSVELLAAISHRDSQKVPQYLYNDLEKVVLPE
HPQVQEIRDLFQSFGALGTMMSGSGPTVFGLADSRSKAELMATQLQTALPDPDLEILVTKFCSSGIQLLS
>Mature_310_residues
MHAYTLKAPAKINLLLEIIGDRPDGFHELVMVMQSVNLADIVELQPLSGDRIELFCNHPQVPCDQSNLAHRAARLMQEQF
PGKGGVAITIDKKIPIGAGLAGGSADAAAVLVGLDLMWGLGLTQAELHTYAAKLGSDIPFCVSGGTALALGRGEELTPLP
DLDQLYAVLAKYRSLSVATPWAYKTYRQQFSESYARDPEALEKRRREGHSVELLAAISHRDSQKVPQYLYNDLEKVVLPE
HPQVQEIRDLFQSFGALGTMMSGSGPTVFGLADSRSKAELMATQLQTALPDPDLEILVTKFCSSGIQLLS

Specific function: Catalyzes the phosphorylation of the position 2 hydroxy group of 4-diphosphocytidyl-2C-methyl-D-erythritol

COG id: COG1947

COG function: function code I; 4-diphosphocytidyl-2C-methyl-D-erythritol 2-phosphate synthase

Gene ontology:
GO:0000166: 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase
GO:0005524: 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase
GO:0008299: 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase
GO:0016114: 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase
GO:0016301: 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase
GO:0016310: 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase
GO:0016740: 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase
GO:0050515: 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GHMP kinase family. IspE subfamily

Homologues:

Organism=Escherichia coli, GI1787459, Length=283, Percent_Identity=31.095406360424, Blast_Score=103, Evalue=1e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPE_ACAM1 (B0CC88)

Other databases:

- EMBL:   CP000828
- RefSeq:   YP_001516087.1
- ProteinModelPortal:   B0CC88
- SMR:   B0CC88
- GeneID:   5680568
- GenomeReviews:   CP000828_GR
- KEGG:   amr:AM1_1752
- HOGENOM:   HBG734593
- OMA:   VSTKEVY
- ProtClustDB:   PRK02534
- BioCyc:   AMAR329726:AM1_1752-MONOMER
- HAMAP:   MF_00061
- InterPro:   IPR006204
- InterPro:   IPR013750
- InterPro:   IPR004424
- InterPro:   IPR020568
- InterPro:   IPR014721
- Gene3D:   G3DSA:3.30.230.10
- PANTHER:   PTHR20861:SF2
- PIRSF:   PIRSF010376
- TIGRFAMs:   TIGR00154

Pfam domain/function: PF08544 GHMP_kinases_C; PF00288 GHMP_kinases_N; SSF54211 Ribosomal_S5_D2-typ_fold

EC number: =2.7.1.148

Molecular weight: Translated: 33695; Mature: 33695

Theoretical pI: Translated: 5.36; Mature: 5.36

Prosite motif: NA

Important sites: ACT_SITE 11-11 ACT_SITE 137-137

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHAYTLKAPAKINLLLEIIGDRPDGFHELVMVMQSVNLADIVELQPLSGDRIELFCNHPQ
CCCEECCCCHHHHHHHHHHCCCCCCHHHHHHHHHHCCHHHHHEECCCCCCEEEEEECCCC
VPCDQSNLAHRAARLMQEQFPGKGGVAITIDKKIPIGAGLAGGSADAAAVLVGLDLMWGL
CCCCHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHCC
GLTQAELHTYAAKLGSDIPFCVSGGTALALGRGEELTPLPDLDQLYAVLAKYRSLSVATP
CCCHHHHHHHHHHHCCCCCEEECCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHCCCCCC
WAYKTYRQQFSESYARDPEALEKRRREGHSVELLAAISHRDSQKVPQYLYNDLEKVVLPE
HHHHHHHHHHHHHHCCCHHHHHHHHHCCCCEEEEEEHHCCCHHHHHHHHHHHHHHHCCCC
HPQVQEIRDLFQSFGALGTMMSGSGPTVFGLADSRSKAELMATQLQTALPDPDLEILVTK
CCCHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHCCCCCHHHHHHH
FCSSGIQLLS
HHHCCHHHCC
>Mature Secondary Structure
MHAYTLKAPAKINLLLEIIGDRPDGFHELVMVMQSVNLADIVELQPLSGDRIELFCNHPQ
CCCEECCCCHHHHHHHHHHCCCCCCHHHHHHHHHHCCHHHHHEECCCCCCEEEEEECCCC
VPCDQSNLAHRAARLMQEQFPGKGGVAITIDKKIPIGAGLAGGSADAAAVLVGLDLMWGL
CCCCHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHCC
GLTQAELHTYAAKLGSDIPFCVSGGTALALGRGEELTPLPDLDQLYAVLAKYRSLSVATP
CCCHHHHHHHHHHHCCCCCEEECCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHCCCCCC
WAYKTYRQQFSESYARDPEALEKRRREGHSVELLAAISHRDSQKVPQYLYNDLEKVVLPE
HHHHHHHHHHHHHHCCCHHHHHHHHHCCCCEEEEEEHHCCCHHHHHHHHHHHHHHHCCCC
HPQVQEIRDLFQSFGALGTMMSGSGPTVFGLADSRSKAELMATQLQTALPDPDLEILVTK
CCCHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHCCCCCHHHHHHH
FCSSGIQLLS
HHHCCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA