Click here to switch to the map view.

The map label for this gene is sphR [H]

Identifier: 158334884

GI number: 158334884

Start: 1706748

End: 1707473

Strand: Reverse

Name: sphR [H]

Synonym: AM1_1721

Alternate gene names: 158334884

Gene position: 1707473-1706748 (Counterclockwise)

Preceding gene: 158334886

Following gene: 158334882

Centisome position: 26.25

GC content: 49.59

Gene sequence:

>726_bases
ATGAGTCAAATTCTGCTAGTTGATGATGAGGTTGAATTAACCGATGCTCTGGGCCGTTTGCTTAAACGGGAAGGGTATAG
CGTTGATGTGGCGAATGATGGCGAAGCGGCCCTACTTGCTGTGCAACAAAAAATAGACCAGGGACAGCTATATGACTTAC
TTATCCTGGATTGGATGTTGCCCAAATTGTCGGGGATTGAAGTGTGTCAGGAGTTGCGATCGCAATCCCAACTCACCCCC
GTTTTATTCCTGACCGCCAAAGATACCCTAGACGATCGGGTACAAGGCTTGGATGCCGGTGCAGATGACTACCTAGTCAA
GCCCTTTGAATTAAGAGAGCTACTGGCACGAGTTCGGGCTCTTCTGCGTCGGAGTGAGCAGTTCTCGCAAACATCGCCGG
AAGCCTTATTCCAGCGCATCCAGATGGAAGGCTTAGAACTGGATTTGGAAAGCCAAATTGCCTACTGTCAAGGACAGGCG
ATCACCTTGTCAGAAAAAGAAAGTCAGCTGCTGACCTATCTGATGCAGCATCCCCACCAGCTGCTTACCCATGACCAGAT
TTACCAACATCTATGGCCAAGTGGAGATCAACCGAGTAGCAATGTCTTAGCCGCCCAAATTCGGCTGCTGCGTCGAAAAA
TTGCCGCCAAAAACTCTCCGCCGCTCATTCATACGATCTATGGCAAAGGATATTGGTTTGGGGCCAAGGTAGAGGCTGAA
CCGTAA

Upstream 100 bases:

>100_bases
GCATAATCCAGTTTGATCCTGCTGGCCTGGGGCAATGCTTTTTGGGGATATCCCCTTAACAGTACCGTAAGCCCTGCTTT
GTGACCTGCTGTGGTGACCA

Downstream 100 bases:

>100_bases
TATTTGTGGTGATGGAATAAAGGATATTTAAAAATAAAAAAGCTCTTGAGAGTCTTGCCGTGTTTCGACCTCAAGAGCTT
TTCATCTGAATCACTCCTCA

Product: two-component transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 241; Mature: 240

Protein sequence:

>241_residues
MSQILLVDDEVELTDALGRLLKREGYSVDVANDGEAALLAVQQKIDQGQLYDLLILDWMLPKLSGIEVCQELRSQSQLTP
VLFLTAKDTLDDRVQGLDAGADDYLVKPFELRELLARVRALLRRSEQFSQTSPEALFQRIQMEGLELDLESQIAYCQGQA
ITLSEKESQLLTYLMQHPHQLLTHDQIYQHLWPSGDQPSSNVLAAQIRLLRRKIAAKNSPPLIHTIYGKGYWFGAKVEAE
P

Sequences:

>Translated_241_residues
MSQILLVDDEVELTDALGRLLKREGYSVDVANDGEAALLAVQQKIDQGQLYDLLILDWMLPKLSGIEVCQELRSQSQLTP
VLFLTAKDTLDDRVQGLDAGADDYLVKPFELRELLARVRALLRRSEQFSQTSPEALFQRIQMEGLELDLESQIAYCQGQA
ITLSEKESQLLTYLMQHPHQLLTHDQIYQHLWPSGDQPSSNVLAAQIRLLRRKIAAKNSPPLIHTIYGKGYWFGAKVEAE
P
>Mature_240_residues
SQILLVDDEVELTDALGRLLKREGYSVDVANDGEAALLAVQQKIDQGQLYDLLILDWMLPKLSGIEVCQELRSQSQLTPV
LFLTAKDTLDDRVQGLDAGADDYLVKPFELRELLARVRALLRRSEQFSQTSPEALFQRIQMEGLELDLESQIAYCQGQAI
TLSEKESQLLTYLMQHPHQLLTHDQIYQHLWPSGDQPSSNVLAAQIRLLRRKIAAKNSPPLIHTIYGKGYWFGAKVEAEP

Specific function: Member of the two-component regulatory system sphR/sphS. Response regulator. Involved in inducible production of alkaline phosphatase in response to phosphate limitation as it is directly involved in the regulation of phoA transcription in response to pho

COG id: COG0745

COG function: function code TK; Response regulators consisting of a CheY-like receiver domain and a winged-helix DNA-binding domain

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1786784, Length=230, Percent_Identity=37.8260869565217, Blast_Score=141, Evalue=3e-35,
Organism=Escherichia coli, GI87082012, Length=234, Percent_Identity=38.4615384615385, Blast_Score=140, Evalue=8e-35,
Organism=Escherichia coli, GI1786599, Length=236, Percent_Identity=32.2033898305085, Blast_Score=139, Evalue=2e-34,
Organism=Escherichia coli, GI1789402, Length=234, Percent_Identity=35.8974358974359, Blast_Score=130, Evalue=8e-32,
Organism=Escherichia coli, GI2367329, Length=233, Percent_Identity=39.9141630901288, Blast_Score=129, Evalue=2e-31,
Organism=Escherichia coli, GI1790552, Length=236, Percent_Identity=34.3220338983051, Blast_Score=124, Evalue=5e-30,
Organism=Escherichia coli, GI1789809, Length=233, Percent_Identity=33.9055793991416, Blast_Score=124, Evalue=7e-30,
Organism=Escherichia coli, GI1790860, Length=232, Percent_Identity=36.2068965517241, Blast_Score=122, Evalue=1e-29,
Organism=Escherichia coli, GI1790863, Length=239, Percent_Identity=30.1255230125523, Blast_Score=112, Evalue=3e-26,
Organism=Escherichia coli, GI1788394, Length=235, Percent_Identity=31.9148936170213, Blast_Score=106, Evalue=1e-24,
Organism=Escherichia coli, GI1787375, Length=231, Percent_Identity=29.004329004329, Blast_Score=105, Evalue=2e-24,
Organism=Escherichia coli, GI1787229, Length=236, Percent_Identity=30.9322033898305, Blast_Score=104, Evalue=5e-24,
Organism=Escherichia coli, GI1786911, Length=234, Percent_Identity=32.0512820512821, Blast_Score=99, Evalue=4e-22,
Organism=Escherichia coli, GI1790437, Length=141, Percent_Identity=31.2056737588652, Blast_Score=75, Evalue=3e-15,
Organism=Escherichia coli, GI1788550, Length=119, Percent_Identity=32.7731092436975, Blast_Score=72, Evalue=2e-14,
Organism=Escherichia coli, GI145693140, Length=240, Percent_Identity=27.5, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI48994928, Length=123, Percent_Identity=37.3983739837398, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI1788713, Length=143, Percent_Identity=33.5664335664336, Blast_Score=62, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001867
- InterPro:   IPR001789
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00072 Response_reg; PF00486 Trans_reg_C [H]

EC number: NA

Molecular weight: Translated: 27255; Mature: 27123

Theoretical pI: Translated: 4.68; Mature: 4.68

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQILLVDDEVELTDALGRLLKREGYSVDVANDGEAALLAVQQKIDQGQLYDLLILDWML
CCCEEEECCCHHHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHCCCCHHHHHHHHHHHH
PKLSGIEVCQELRSQSQLTPVLFLTAKDTLDDRVQGLDAGADDYLVKPFELRELLARVRA
CCCCHHHHHHHHHHHCCCCEEEEEEECHHHHHHHHCCCCCCCCCEECCHHHHHHHHHHHH
LLRRSEQFSQTSPEALFQRIQMEGLELDLESQIAYCQGQAITLSEKESQLLTYLMQHPHQ
HHHHHHHHCCCCHHHHHHHHHHCCCCCCHHHHHHHHCCCEEEECCHHHHHHHHHHHCHHH
LLTHDQIYQHLWPSGDQPSSNVLAAQIRLLRRKIAAKNSPPLIHTIYGKGYWFGAKVEAE
HHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCEEECCEECCC
P
C
>Mature Secondary Structure 
SQILLVDDEVELTDALGRLLKREGYSVDVANDGEAALLAVQQKIDQGQLYDLLILDWML
CCEEEECCCHHHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHCCCCHHHHHHHHHHHH
PKLSGIEVCQELRSQSQLTPVLFLTAKDTLDDRVQGLDAGADDYLVKPFELRELLARVRA
CCCCHHHHHHHHHHHCCCCEEEEEEECHHHHHHHHCCCCCCCCCEECCHHHHHHHHHHHH
LLRRSEQFSQTSPEALFQRIQMEGLELDLESQIAYCQGQAITLSEKESQLLTYLMQHPHQ
HHHHHHHHCCCCHHHHHHHHHHCCCCCCHHHHHHHHCCCEEEECCHHHHHHHHHHHCHHH
LLTHDQIYQHLWPSGDQPSSNVLAAQIRLLRRKIAAKNSPPLIHTIYGKGYWFGAKVEAE
HHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCEEECCEECCC
P
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8497200; 8157591 [H]