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The map label for this gene is tilS [H]

Identifier: 158334883

GI number: 158334883

Start: 1705196

End: 1706212

Strand: Direct

Name: tilS [H]

Synonym: AM1_1720

Alternate gene names: 158334883

Gene position: 1705196-1706212 (Clockwise)

Preceding gene: 158334881

Following gene: 158334885

Centisome position: 26.22

GC content: 49.36

Gene sequence:

>1017_bases
GTGATTAATTCTCCTCCTTGGACTCCACTCCATGCTCATCTGCACCACATCCTGAAAGTACGGCAACTCTTGCCCATACA
ACAGCGAATTTTAATTGCTCTTTCGGGTGGGCAAGATTCATTTTGCCTCTTGCAGCTTTTAGTCGACCTACAGCCCAAAT
GGGGTTGGCAACTAGCCATTGCCCATGGTGATCATCGCTGGCCTCTAGATTCTGATGCTAACGCCCACCATGTTACTCAA
ATTGCCCGCCAATATCAGTTGCCCTACTTCTTACGCCAAGCCACCACGGAACTCAGAAGCGAAGCCGCTGGACGCCAATG
GCGATATCAGATCCTGACAGAGATTGCCGAACAGGAAGCATATCCCCTGGTAGTCTCCGCCCATACCGCCAGCGATCGCG
CCGAGACCTTACTCTATAACTTAATACGAGGTAGCGGCACAGATGGCTTACAAGCTTTAAGTTGGCAGCGTCCTCTCTCA
GATCGGGTGCAGCTTGTACGGCCATTGCTCGACGTGGCACGAGCCCAAACGGGAGACTTTTGTCGGCACAGAAATCTGCC
GATCTGGCAAGATGCCATGAATCAAAACCTCGTCTATCGTCGTAACCGCATTCGGCTAGAAGTTCTCCCTCATCTGAAGA
CCCATTTCAATCCTCAAGTGGAAACCACCCTGGCCCATACTGCAGAAATCTTGCAAGGTGATGTTGATTTTCTAGAAGCA
GAAACCCAGAAACTATTGGCGATTGCCCTGCCTAGTCCCCCTCCGGTCTTATCTGAAACAGAACAATATTGGGGGTATAT
TAATCGGGATGCCCTCCAATCTGCTCCCCTGGCGTTACAACGCCGAGCCCTACGTCATTTTTTGCGGCAGGCTATCGATG
TATCTGCTAATTTCCAACAAATCGAAAAATTGATGGCTTTAGTGAAAGCACCGAATCATTCCCGCACCGATCCGTTCAAA
AAACAATGGGTAGCAGAAGTTATTAAACCCTGGATAGTGATTCGGTCTCTTTCATAA

Upstream 100 bases:

>100_bases
TAGACTTTACTCTAGTCTTAGAAAAATGCTTTCAACCCTTAGGTTTATCTTCAATAAATTGGGTCTTCCCCAAGATTAAG
CCTCCTCCCATAGACCTGTC

Downstream 100 bases:

>100_bases
CGGCAGTTTCCCTAAGAAATGGTGCAGAAGAAACGTTTACATCCAGTGCAGGCGACAGGGACAATTAATGGGCTAGACCT
TTTACTGTCTGCAATACAAG

Product: tRNA(Ile)-lysidine synthase

Products: NA

Alternate protein names: tRNA(Ile)-2-lysyl-cytidine synthase; tRNA(Ile)-lysidine synthetase [H]

Number of amino acids: Translated: 338; Mature: 338

Protein sequence:

>338_residues
MINSPPWTPLHAHLHHILKVRQLLPIQQRILIALSGGQDSFCLLQLLVDLQPKWGWQLAIAHGDHRWPLDSDANAHHVTQ
IARQYQLPYFLRQATTELRSEAAGRQWRYQILTEIAEQEAYPLVVSAHTASDRAETLLYNLIRGSGTDGLQALSWQRPLS
DRVQLVRPLLDVARAQTGDFCRHRNLPIWQDAMNQNLVYRRNRIRLEVLPHLKTHFNPQVETTLAHTAEILQGDVDFLEA
ETQKLLAIALPSPPPVLSETEQYWGYINRDALQSAPLALQRRALRHFLRQAIDVSANFQQIEKLMALVKAPNHSRTDPFK
KQWVAEVIKPWIVIRSLS

Sequences:

>Translated_338_residues
MINSPPWTPLHAHLHHILKVRQLLPIQQRILIALSGGQDSFCLLQLLVDLQPKWGWQLAIAHGDHRWPLDSDANAHHVTQ
IARQYQLPYFLRQATTELRSEAAGRQWRYQILTEIAEQEAYPLVVSAHTASDRAETLLYNLIRGSGTDGLQALSWQRPLS
DRVQLVRPLLDVARAQTGDFCRHRNLPIWQDAMNQNLVYRRNRIRLEVLPHLKTHFNPQVETTLAHTAEILQGDVDFLEA
ETQKLLAIALPSPPPVLSETEQYWGYINRDALQSAPLALQRRALRHFLRQAIDVSANFQQIEKLMALVKAPNHSRTDPFK
KQWVAEVIKPWIVIRSLS
>Mature_338_residues
MINSPPWTPLHAHLHHILKVRQLLPIQQRILIALSGGQDSFCLLQLLVDLQPKWGWQLAIAHGDHRWPLDSDANAHHVTQ
IARQYQLPYFLRQATTELRSEAAGRQWRYQILTEIAEQEAYPLVVSAHTASDRAETLLYNLIRGSGTDGLQALSWQRPLS
DRVQLVRPLLDVARAQTGDFCRHRNLPIWQDAMNQNLVYRRNRIRLEVLPHLKTHFNPQVETTLAHTAEILQGDVDFLEA
ETQKLLAIALPSPPPVLSETEQYWGYINRDALQSAPLALQRRALRHFLRQAIDVSANFQQIEKLMALVKAPNHSRTDPFK
KQWVAEVIKPWIVIRSLS

Specific function: Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionin

COG id: COG0037

COG function: function code D; Predicted ATPase of the PP-loop superfamily implicated in cell cycle control

Gene ontology:
GO:0000166: tRNA(Ile)-lysidine synthase
GO:0005524: tRNA(Ile)-lysidine synthase
GO:0005737: tRNA(Ile)-lysidine synthase
GO:0006400: tRNA(Ile)-lysidine synthase
GO:0008033: tRNA(Ile)-lysidine synthase
GO:0016874: tRNA(Ile)-lysidine synthase
GO:0016879: tRNA(Ile)-lysidine synthase

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA(Ile)-lysidine synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786386, Length=216, Percent_Identity=34.2592592592593, Blast_Score=83, Evalue=3e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012094
- InterPro:   IPR012795
- InterPro:   IPR014729
- InterPro:   IPR011063 [H]

Pfam domain/function: PF01171 ATP_bind_3 [H]

EC number: NA

Molecular weight: Translated: 38858; Mature: 38858

Theoretical pI: Translated: 9.55; Mature: 9.55

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MINSPPWTPLHAHLHHILKVRQLLPIQQRILIALSGGQDSFCLLQLLVDLQPKWGWQLAI
CCCCCCCCHHHHHHHHHHHHHHHCCHHHEEEEEEECCCCHHHHHHHHHHCCCCCCEEEEE
AHGDHRWPLDSDANAHHVTQIARQYQLPYFLRQATTELRSEAAGRQWRYQILTEIAEQEA
EECCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCC
YPLVVSAHTASDRAETLLYNLIRGSGTDGLQALSWQRPLSDRVQLVRPLLDVARAQTGDF
CCEEEEECCCHHHHHHHHHHHHHCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCHH
CRHRNLPIWQDAMNQNLVYRRNRIRLEVLPHLKTHFNPQVETTLAHTAEILQGDVDFLEA
HHHCCCCCHHHHHCCCCEEHHCCEEEEEEHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHH
ETQKLLAIALPSPPPVLSETEQYWGYINRDALQSAPLALQRRALRHFLRQAIDVSANFQQ
HHHHEEEEECCCCCCCCCCCHHHCCCCCHHHHHHCCHHHHHHHHHHHHHHHHCCCCCHHH
IEKLMALVKAPNHSRTDPFKKQWVAEVIKPWIVIRSLS
HHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MINSPPWTPLHAHLHHILKVRQLLPIQQRILIALSGGQDSFCLLQLLVDLQPKWGWQLAI
CCCCCCCCHHHHHHHHHHHHHHHCCHHHEEEEEEECCCCHHHHHHHHHHCCCCCCEEEEE
AHGDHRWPLDSDANAHHVTQIARQYQLPYFLRQATTELRSEAAGRQWRYQILTEIAEQEA
EECCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCC
YPLVVSAHTASDRAETLLYNLIRGSGTDGLQALSWQRPLSDRVQLVRPLLDVARAQTGDF
CCEEEEECCCHHHHHHHHHHHHHCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCHH
CRHRNLPIWQDAMNQNLVYRRNRIRLEVLPHLKTHFNPQVETTLAHTAEILQGDVDFLEA
HHHCCCCCHHHHHCCCCEEHHCCEEEEEEHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHH
ETQKLLAIALPSPPPVLSETEQYWGYINRDALQSAPLALQRRALRHFLRQAIDVSANFQQ
HHHHEEEEECCCCCCCCCCCHHHCCCCCHHHHHHCCHHHHHHHHHHHHHHHHCCCCCHHH
IEKLMALVKAPNHSRTDPFKKQWVAEVIKPWIVIRSLS
HHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11759840 [H]