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The map label for this gene is pbpF [H]

Identifier: 158334866

GI number: 158334866

Start: 1683514

End: 1685799

Strand: Reverse

Name: pbpF [H]

Synonym: AM1_1703

Alternate gene names: 158334866

Gene position: 1685799-1683514 (Counterclockwise)

Preceding gene: 158334869

Following gene: 158334856

Centisome position: 25.92

GC content: 51.09

Gene sequence:

>2286_bases
GTGGCCAAACAAATTTCAGAAATTCGTCCTTCTGTTGATTCACGATCGTCTCAAGTCCTTCCTTCCAAGCTGGGCAAAGC
TAAAGATCGATCCCTCAAGCTGGACCCGATACCGGAGTCGACGGATAGTCTCCGTAAAAAATTAAAGGATAAACGACGCC
AGCAGAAGCAACAAGAAAAGCAACGAAAACAGGCACTCCGGCACAAATCAAAACGCCGTAAAACTAGTCCTCGACGGATT
GCTGCCTCTAATCCAGATTCTAAGCCCTCAGAAAAAGAGCTAGTGAAGGCCTTAGCTGTTCCGGTCTCCGTCGAGACAGT
ACCGGTTCGTCAGCGTCCTAAAAGGGTGGTTCGTCGCTCAACCTCGTCCCGATGGCGGCCGTTAAAACGCCTAGGAATTT
TGCTAGGGGTAACTGGTGTGGCTTGTGCAGGGGGATGGGTTGCCCTTGAACTGAATCTGCCAGATACGACCCATATGGAA
ACTGAAACCCAGGTCCGTGCCGGGACGCTCACCCTAAAGTCTGCAGATGGTTCTATCCTGTTTCAAGATGGACCGGCGAG
TCATAAAAAGGTCAAGATCGATCAAATCCCTCGGCAATTGCAGCAGGCCTTTATTGCCACGGAAGATCGACGGTTTTTTG
AGCATGATGGGATTGATGGCCAGGGTATTTTACGGGCCATGGTCACTAATGTGTTGTCCGGGGAGCTAGCGGAGGGGGGA
AGTACGATTACCCAACAGCTGGCACGGATGACTTATCTCAATCAAGAACGGTCGATTCTGCGGAAGCTGAAGGAAGCCCG
TTTATCCCAAAAAATTGAAGATAAACTCTCTAAGACCCAGATCCTGGAACGATACCTGAATCAGGTGTATCTCGGAAGTG
GGGCTTATGGGGTGGCCGATGCCGCTTGGGTTTATTTTGGCAAGTCGTTAGATCAGCTTAATTTGCAGGAAAGTGCGACC
CTGGCAGGATTGCCCGCCGCGCCAAGTTTGTACTCGCCATTAAGTAATCCCAAAGTGGCCAAGGAACGACGGAACTTAGT
GCTTGAGCGGATGGTCCGAGCTGAGGCGATTACGCCTGACATGGCTGCGAAAGCAAAGAAAAGTCCCATTGCTTTAAATC
CGAAAGTGCCCCCCAATGCCCAAGACCACAGCCCATACTTTACGGCGTATCTCCAGCAGCAGTTGCCAGAACTGTTATCG
AAGGACGTGCTGGCACAGGGGGGATTAGAAATTGAAACGACCCTAAACTTAAAGTGGCAAAAAGCGGCTGCCTCAACCAT
CAAAGATGCGGTGTACCACGATGGCTATGCCCAACGGTTTGATCAGGCGGCTTTGGTCTCCCTTGATCCCCGCAATGGTG
AAATCAAGACAATGGTGGGCGGCAATGCGTTTGAACAATCTCAATTTAACCGGGTGACGCAATCCCGGCGGCAGCCTGGA
TCGACCTTTAAAGCGCTTGTATACACGGCTGCGATCGCATCAGGATTTTCTCCTCATGATGGTTTTCCAGATGCTCCCTT
TATTGTGGATGGCTACCAACCCAATAATTATGGCCATGTCTATAAGGGCTGGATGTCTATGAACCAGGCACTGACCAAGT
CTGCGAATATCCCCGCAGTACGAGTCTTTATGGCCGTTGGCTTTGAACCCACCATTAAGCTGGCCCGAGAATTGGGCATT
CAATCTCCCTTAAAGCCCTATTACTCCACAGCGTTAGGGGCGAATGAAGTCAGCTTATTGGAATTGACCCGAGCCTACGG
TACCCTTGCTGCCCAAGGCTATCGAACCCAGCCCTATGGGATTCGCTCCATTCGCAATCGTCAAGGTAAAGTCCTGTTCC
AACATAAAACGGCTAAGCAGCGCGTCCTTGATTCATCCTCATCGGCCATTATGACTTGGATGTTGCAACAGGTTGTCACG
ACCGGAACGGGCAAGCCGGCCCAAATTGGTCGTCCCGTTGCCGGCAAGACAGGCACCTCTGAACATAACCGGGATTTATG
GTTTGTTGGGTATATTCCGCAGCTGGTAACTGGGGTCTGGTTGGGGAATGACGATAACTACCCCACCACTGGGAGTAGTG
CAACTGCTGCGGCCACTTGGGGAAACTTTATGAAGCAGACAGTCAAAGATATTCCTGCTCAGTCTTTCCCCGCCTTGCCA
AAATTGGCAGGACGCAAGGGCAGCATTAAACCTAAGTCTGTTAAGCCGAAACGGATCAGGAATTTACCCATGCCCCAGCA
GGAAGAGCCTGCGACAGAGCCAGCCGAGCGCGAGACTTTAGACTAA

Upstream 100 bases:

>100_bases
TTATTTAGAGACATTGCGTTAAATTAAGTCCCAAGTTGCAAGGATCTGGTGAGCATAGAGCAGAGCCAATCGGTATTGAT
TACAGCAAGAGGGGCGTTGC

Downstream 100 bases:

>100_bases
ACCTATCCCCATTGCCTGTTTCAATCGGGCGCGATGTATTTCGCACCCGATCTGGTATTAAGTAGAGGCAGAGATCTCAG
AACGGTTAGAGCTACGCCGC

Product: penicillin-binding protein 1A

Products: NA

Alternate protein names: PBP-1F; Penicillin-binding protein F [H]

Number of amino acids: Translated: 761; Mature: 760

Protein sequence:

>761_residues
MAKQISEIRPSVDSRSSQVLPSKLGKAKDRSLKLDPIPESTDSLRKKLKDKRRQQKQQEKQRKQALRHKSKRRKTSPRRI
AASNPDSKPSEKELVKALAVPVSVETVPVRQRPKRVVRRSTSSRWRPLKRLGILLGVTGVACAGGWVALELNLPDTTHME
TETQVRAGTLTLKSADGSILFQDGPASHKKVKIDQIPRQLQQAFIATEDRRFFEHDGIDGQGILRAMVTNVLSGELAEGG
STITQQLARMTYLNQERSILRKLKEARLSQKIEDKLSKTQILERYLNQVYLGSGAYGVADAAWVYFGKSLDQLNLQESAT
LAGLPAAPSLYSPLSNPKVAKERRNLVLERMVRAEAITPDMAAKAKKSPIALNPKVPPNAQDHSPYFTAYLQQQLPELLS
KDVLAQGGLEIETTLNLKWQKAAASTIKDAVYHDGYAQRFDQAALVSLDPRNGEIKTMVGGNAFEQSQFNRVTQSRRQPG
STFKALVYTAAIASGFSPHDGFPDAPFIVDGYQPNNYGHVYKGWMSMNQALTKSANIPAVRVFMAVGFEPTIKLARELGI
QSPLKPYYSTALGANEVSLLELTRAYGTLAAQGYRTQPYGIRSIRNRQGKVLFQHKTAKQRVLDSSSSAIMTWMLQQVVT
TGTGKPAQIGRPVAGKTGTSEHNRDLWFVGYIPQLVTGVWLGNDDNYPTTGSSATAAATWGNFMKQTVKDIPAQSFPALP
KLAGRKGSIKPKSVKPKRIRNLPMPQQEEPATEPAERETLD

Sequences:

>Translated_761_residues
MAKQISEIRPSVDSRSSQVLPSKLGKAKDRSLKLDPIPESTDSLRKKLKDKRRQQKQQEKQRKQALRHKSKRRKTSPRRI
AASNPDSKPSEKELVKALAVPVSVETVPVRQRPKRVVRRSTSSRWRPLKRLGILLGVTGVACAGGWVALELNLPDTTHME
TETQVRAGTLTLKSADGSILFQDGPASHKKVKIDQIPRQLQQAFIATEDRRFFEHDGIDGQGILRAMVTNVLSGELAEGG
STITQQLARMTYLNQERSILRKLKEARLSQKIEDKLSKTQILERYLNQVYLGSGAYGVADAAWVYFGKSLDQLNLQESAT
LAGLPAAPSLYSPLSNPKVAKERRNLVLERMVRAEAITPDMAAKAKKSPIALNPKVPPNAQDHSPYFTAYLQQQLPELLS
KDVLAQGGLEIETTLNLKWQKAAASTIKDAVYHDGYAQRFDQAALVSLDPRNGEIKTMVGGNAFEQSQFNRVTQSRRQPG
STFKALVYTAAIASGFSPHDGFPDAPFIVDGYQPNNYGHVYKGWMSMNQALTKSANIPAVRVFMAVGFEPTIKLARELGI
QSPLKPYYSTALGANEVSLLELTRAYGTLAAQGYRTQPYGIRSIRNRQGKVLFQHKTAKQRVLDSSSSAIMTWMLQQVVT
TGTGKPAQIGRPVAGKTGTSEHNRDLWFVGYIPQLVTGVWLGNDDNYPTTGSSATAAATWGNFMKQTVKDIPAQSFPALP
KLAGRKGSIKPKSVKPKRIRNLPMPQQEEPATEPAERETLD
>Mature_760_residues
AKQISEIRPSVDSRSSQVLPSKLGKAKDRSLKLDPIPESTDSLRKKLKDKRRQQKQQEKQRKQALRHKSKRRKTSPRRIA
ASNPDSKPSEKELVKALAVPVSVETVPVRQRPKRVVRRSTSSRWRPLKRLGILLGVTGVACAGGWVALELNLPDTTHMET
ETQVRAGTLTLKSADGSILFQDGPASHKKVKIDQIPRQLQQAFIATEDRRFFEHDGIDGQGILRAMVTNVLSGELAEGGS
TITQQLARMTYLNQERSILRKLKEARLSQKIEDKLSKTQILERYLNQVYLGSGAYGVADAAWVYFGKSLDQLNLQESATL
AGLPAAPSLYSPLSNPKVAKERRNLVLERMVRAEAITPDMAAKAKKSPIALNPKVPPNAQDHSPYFTAYLQQQLPELLSK
DVLAQGGLEIETTLNLKWQKAAASTIKDAVYHDGYAQRFDQAALVSLDPRNGEIKTMVGGNAFEQSQFNRVTQSRRQPGS
TFKALVYTAAIASGFSPHDGFPDAPFIVDGYQPNNYGHVYKGWMSMNQALTKSANIPAVRVFMAVGFEPTIKLARELGIQ
SPLKPYYSTALGANEVSLLELTRAYGTLAAQGYRTQPYGIRSIRNRQGKVLFQHKTAKQRVLDSSSSAIMTWMLQQVVTT
GTGKPAQIGRPVAGKTGTSEHNRDLWFVGYIPQLVTGVWLGNDDNYPTTGSSATAAATWGNFMKQTVKDIPAQSFPALPK
LAGRKGSIKPKSVKPKRIRNLPMPQQEEPATEPAERETLD

Specific function: Cell wall formation. May be involved in outgrowth of the germinated spore or it could function in the synthesis of the germ cell wall [H]

COG id: COG0744

COG function: function code M; Membrane carboxypeptidase (penicillin-binding protein)

Gene ontology:

Cell location: Cell membrane; Single-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the transpeptidase family [H]

Homologues:

Organism=Escherichia coli, GI1786343, Length=526, Percent_Identity=32.8897338403042, Blast_Score=250, Evalue=3e-67,
Organism=Escherichia coli, GI87082258, Length=305, Percent_Identity=40.655737704918, Blast_Score=206, Evalue=3e-54,
Organism=Escherichia coli, GI1788867, Length=549, Percent_Identity=30.0546448087432, Blast_Score=180, Evalue=3e-46,
Organism=Escherichia coli, GI1789601, Length=161, Percent_Identity=37.2670807453416, Blast_Score=87, Evalue=3e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR001264
- InterPro:   IPR011816
- InterPro:   IPR001460 [H]

Pfam domain/function: PF00912 Transgly; PF00905 Transpeptidase [H]

EC number: 2.4.1.129; 3.4.-.- [C]

Molecular weight: Translated: 84118; Mature: 83987

Theoretical pI: Translated: 10.66; Mature: 10.66

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.1 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.1 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKQISEIRPSVDSRSSQVLPSKLGKAKDRSLKLDPIPESTDSLRKKLKDKRRQQKQQEK
CCCCHHHHCCCCCCCCCCCCHHHHCCCCCCCEECCCCCCCHHHHHHHHHHHHHHHHHHHH
QRKQALRHKSKRRKTSPRRIAASNPDSKPSEKELVKALAVPVSVETVPVRQRPKRVVRRS
HHHHHHHHHHHHHCCCCCEEECCCCCCCCCHHHHHHHHHCCCEEEECCCHHHHHHHHHHC
TSSRWRPLKRLGILLGVTGVACAGGWVALELNLPDTTHMETETQVRAGTLTLKSADGSIL
CHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCEEEEEEEEEECCCEEE
FQDGPASHKKVKIDQIPRQLQQAFIATEDRRFFEHDGIDGQGILRAMVTNVLSGELAEGG
ECCCCCCCCEEEHHHHHHHHHHHHHHHHCHHHHHCCCCCHHHHHHHHHHHHHHCHHHCCC
STITQQLARMTYLNQERSILRKLKEARLSQKIEDKLSKTQILERYLNQVYLGSGAYGVAD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCHHH
AAWVYFGKSLDQLNLQESATLAGLPAAPSLYSPLSNPKVAKERRNLVLERMVRAEAITPD
HHHHHHCCCHHHCCCHHCCCCCCCCCCCHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCH
MAAKAKKSPIALNPKVPPNAQDHSPYFTAYLQQQLPELLSKDVLAQGGLEIETTLNLKWQ
HHHHCCCCCEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECEEEH
KAAASTIKDAVYHDGYAQRFDQAALVSLDPRNGEIKTMVGGNAFEQSQFNRVTQSRRQPG
HHHHHHHHHHHHCCHHHHHHCCCEEEEECCCCCCEEEEECCCHHHHHHHHHHHHHHCCCC
STFKALVYTAAIASGFSPHDGFPDAPFIVDGYQPNNYGHVYKGWMSMNQALTKSANIPAV
HHHHHHHHHHHHHCCCCCCCCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCCE
RVFMAVGFEPTIKLARELGIQSPLKPYYSTALGANEVSLLELTRAYGTLAAQGYRTQPYG
EHHHHHCCCHHHHHHHHCCCCCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCC
IRSIRNRQGKVLFQHKTAKQRVLDSSSSAIMTWMLQQVVTTGTGKPAQIGRPVAGKTGTS
HHHHHCCCCCEEEEHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCHHCCCCCCCCCCCC
EHNRDLWFVGYIPQLVTGVWLGNDDNYPTTGSSATAAATWGNFMKQTVKDIPAQSFPALP
CCCCCEEEEECHHHHHHEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCH
KLAGRKGSIKPKSVKPKRIRNLPMPQQEEPATEPAERETLD
HHCCCCCCCCCCCCCHHHHHCCCCCCCCCCCCCCCHHCCCC
>Mature Secondary Structure 
AKQISEIRPSVDSRSSQVLPSKLGKAKDRSLKLDPIPESTDSLRKKLKDKRRQQKQQEK
CCCHHHHCCCCCCCCCCCCHHHHCCCCCCCEECCCCCCCHHHHHHHHHHHHHHHHHHHH
QRKQALRHKSKRRKTSPRRIAASNPDSKPSEKELVKALAVPVSVETVPVRQRPKRVVRRS
HHHHHHHHHHHHHCCCCCEEECCCCCCCCCHHHHHHHHHCCCEEEECCCHHHHHHHHHHC
TSSRWRPLKRLGILLGVTGVACAGGWVALELNLPDTTHMETETQVRAGTLTLKSADGSIL
CHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCEEEEEEEEEECCCEEE
FQDGPASHKKVKIDQIPRQLQQAFIATEDRRFFEHDGIDGQGILRAMVTNVLSGELAEGG
ECCCCCCCCEEEHHHHHHHHHHHHHHHHCHHHHHCCCCCHHHHHHHHHHHHHHCHHHCCC
STITQQLARMTYLNQERSILRKLKEARLSQKIEDKLSKTQILERYLNQVYLGSGAYGVAD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCHHH
AAWVYFGKSLDQLNLQESATLAGLPAAPSLYSPLSNPKVAKERRNLVLERMVRAEAITPD
HHHHHHCCCHHHCCCHHCCCCCCCCCCCHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCH
MAAKAKKSPIALNPKVPPNAQDHSPYFTAYLQQQLPELLSKDVLAQGGLEIETTLNLKWQ
HHHHCCCCCEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECEEEH
KAAASTIKDAVYHDGYAQRFDQAALVSLDPRNGEIKTMVGGNAFEQSQFNRVTQSRRQPG
HHHHHHHHHHHHCCHHHHHHCCCEEEEECCCCCCEEEEECCCHHHHHHHHHHHHHHCCCC
STFKALVYTAAIASGFSPHDGFPDAPFIVDGYQPNNYGHVYKGWMSMNQALTKSANIPAV
HHHHHHHHHHHHHCCCCCCCCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCCE
RVFMAVGFEPTIKLARELGIQSPLKPYYSTALGANEVSLLELTRAYGTLAAQGYRTQPYG
EHHHHHCCCHHHHHHHHCCCCCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCC
IRSIRNRQGKVLFQHKTAKQRVLDSSSSAIMTWMLQQVVTTGTGKPAQIGRPVAGKTGTS
HHHHHCCCCCEEEEHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCHHCCCCCCCCCCCC
EHNRDLWFVGYIPQLVTGVWLGNDDNYPTTGSSATAAATWGNFMKQTVKDIPAQSFPALP
CCCCCEEEEECHHHHHHEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCH
KLAGRKGSIKPKSVKPKRIRNLPMPQQEEPATEPAERETLD
HHCCCCCCCCCCCCCHHHHHCCCCCCCCCCCCCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Acyltransferases; Aminoacyltransferases [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9579061; 9384377; 8335642; 1459957 [H]