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The map label for this gene is lipA [H]

Identifier: 158334861

GI number: 158334861

Start: 1678192

End: 1679040

Strand: Direct

Name: lipA [H]

Synonym: AM1_1698

Alternate gene names: 158334861

Gene position: 1678192-1679040 (Clockwise)

Preceding gene: 158334860

Following gene: 158334862

Centisome position: 25.8

GC content: 51.59

Gene sequence:

>849_bases
TTGCCAGAGTGGCTACGCCGCCCGATTGGCAAGGCCAGTGAGCTATCCACCGTCCAGCAAATCGTCAAACGCCATAATAT
CCACACTATTTGTGAAGAAGGCCGATGTCCTAATCGGGGCGAATGCTATGCCCAAAAAACGGCCACGTTTTTACTCATGG
GACCTGTCTGCACCCGGGCCTGTTCGTTTTGCCAGGTTGATAAGGGCCATACCCCCCAACCCTTGAATGAACAAGAACCT
CAGAAGGTCGCTGATTCTGTACGGTTACTGGGTTTGAAATATGTAGTACTCACTTCAGTGGCTCGAGATGATTTACCCGA
TCAAGGGGCGGGATGGTTTGCCGCAACCATGGCCGCCATTCGAACCGTCAGCCCCCATACCGATATTGAAGTGCTAACCC
CTGATTTTCGAGGCGATCTCAACTTGATTGCCACCGTTGTTGCTGCCCGACCCGTTTGCTACAACCACAACCTGGAAACG
GTTCGACGCCTTCAAGGACCAGTACGACGAGGAGCAAAATATGAGCGTTCGCTCCAGGTACTAGCCACCGTTAAGGAACT
TGCACCTAAAAATGATGTCATACCTAGTATTGCTACCAAATCTGGTCTAATGCTGGGATTGGGAGAAACGGAAACTGAAA
TCATCGAAACGATGGAGGACCTAAGGTCAGTGGGATGCGACCGCATCACCTTAGGTCAATATATGCGCCCGTCCCTCAAC
CATCTCCCGGTCCAGAAATACTGGCATCCCGAGGAGTTTGATCGCCTAGGTGAGATTGCTCGCTCTCTCGGATTTAGCCA
CGTCCGGTCTGGCCCTTTAGTCAGAAGTTCATACCACGCTGGAGAGTAA

Upstream 100 bases:

>100_bases
GACAAACTCGTTATAGTGATTACCTAATGCATGCGTTTCTTCCATGCTCTCAGGTAGTCAGAAGGCAGACCCCATGGCGA
ATACAGAAATAAGAGAACGC

Downstream 100 bases:

>100_bases
GCTCCCGACTATCGCTGGAAGCATAGATGAAGGATGTTACCGCTCAAACTAACGCCATTGCTGATTGTCTCAAAACCTTG
GAGCAAGTCTCCGCAATGGT

Product: lipoyl synthase

Products: NA

Alternate protein names: Lip-syn 2; Lipoate synthase 2; Lipoic acid synthase 2; Sulfur insertion protein lipA2 [H]

Number of amino acids: Translated: 282; Mature: 281

Protein sequence:

>282_residues
MPEWLRRPIGKASELSTVQQIVKRHNIHTICEEGRCPNRGECYAQKTATFLLMGPVCTRACSFCQVDKGHTPQPLNEQEP
QKVADSVRLLGLKYVVLTSVARDDLPDQGAGWFAATMAAIRTVSPHTDIEVLTPDFRGDLNLIATVVAARPVCYNHNLET
VRRLQGPVRRGAKYERSLQVLATVKELAPKNDVIPSIATKSGLMLGLGETETEIIETMEDLRSVGCDRITLGQYMRPSLN
HLPVQKYWHPEEFDRLGEIARSLGFSHVRSGPLVRSSYHAGE

Sequences:

>Translated_282_residues
MPEWLRRPIGKASELSTVQQIVKRHNIHTICEEGRCPNRGECYAQKTATFLLMGPVCTRACSFCQVDKGHTPQPLNEQEP
QKVADSVRLLGLKYVVLTSVARDDLPDQGAGWFAATMAAIRTVSPHTDIEVLTPDFRGDLNLIATVVAARPVCYNHNLET
VRRLQGPVRRGAKYERSLQVLATVKELAPKNDVIPSIATKSGLMLGLGETETEIIETMEDLRSVGCDRITLGQYMRPSLN
HLPVQKYWHPEEFDRLGEIARSLGFSHVRSGPLVRSSYHAGE
>Mature_281_residues
PEWLRRPIGKASELSTVQQIVKRHNIHTICEEGRCPNRGECYAQKTATFLLMGPVCTRACSFCQVDKGHTPQPLNEQEPQ
KVADSVRLLGLKYVVLTSVARDDLPDQGAGWFAATMAAIRTVSPHTDIEVLTPDFRGDLNLIATVVAARPVCYNHNLETV
RRLQGPVRRGAKYERSLQVLATVKELAPKNDVIPSIATKSGLMLGLGETETEIIETMEDLRSVGCDRITLGQYMRPSLNH
LPVQKYWHPEEFDRLGEIARSLGFSHVRSGPLVRSSYHAGE

Specific function: Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives [H]

COG id: COG0320

COG function: function code H; Lipoate synthase

Gene ontology:
GO:0003824: Lipoic acid synthetase
GO:0005737: Lipoic acid synthetase
GO:0008152: Lipoic acid synthetase
GO:0009107: Lipoic acid synthetase
GO:0009249: Lipoic acid synthetase
GO:0016783: Lipoic acid synthetase
GO:0016992: Lipoic acid synthetase
GO:0051536: Lipoic acid synthetase
GO:0051539: Lipoic acid synthetase

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Lipoyl synthase family [H]

Homologues:

Organism=Homo sapiens, GI37577166, Length=288, Percent_Identity=45.1388888888889, Blast_Score=247, Evalue=9e-66,
Organism=Homo sapiens, GI37577164, Length=251, Percent_Identity=44.2231075697211, Blast_Score=207, Evalue=6e-54,
Organism=Escherichia coli, GI1786846, Length=281, Percent_Identity=46.2633451957295, Blast_Score=257, Evalue=6e-70,
Organism=Caenorhabditis elegans, GI32564533, Length=291, Percent_Identity=43.298969072165, Blast_Score=228, Evalue=3e-60,
Organism=Saccharomyces cerevisiae, GI6324770, Length=288, Percent_Identity=45.4861111111111, Blast_Score=253, Evalue=2e-68,
Organism=Drosophila melanogaster, GI221513272, Length=288, Percent_Identity=44.0972222222222, Blast_Score=240, Evalue=8e-64,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006638
- InterPro:   IPR003698
- InterPro:   IPR007197 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: =2.8.1.8 [H]

Molecular weight: Translated: 31409; Mature: 31278

Theoretical pI: Translated: 8.02; Mature: 8.02

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPEWLRRPIGKASELSTVQQIVKRHNIHTICEEGRCPNRGECYAQKTATFLLMGPVCTRA
CCHHHHHHCCCHHHHHHHHHHHHHCCHHHHHHCCCCCCCCCHHHHHHHHHEEECHHHHHH
CSFCQVDKGHTPQPLNEQEPQKVADSVRLLGLKYVVLTSVARDDLPDQGAGWFAATMAAI
HHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHH
RTVSPHTDIEVLTPDFRGDLNLIATVVAARPVCYNHNLETVRRLQGPVRRGAKYERSLQV
HHCCCCCCEEEECCCCCCCHHHHHHHHHHCCHHCCCCHHHHHHHHHHHHHCHHHHHHHHH
LATVKELAPKNDVIPSIATKSGLMLGLGETETEIIETMEDLRSVGCDRITLGQYMRPSLN
HHHHHHHCCCCCCCCCHHCCCCCEEECCCHHHHHHHHHHHHHHCCCCEEEHHHHHCCCCC
HLPVQKYWHPEEFDRLGEIARSLGFSHVRSGPLVRSSYHAGE
CCCCHHCCCHHHHHHHHHHHHHHCHHHHCCCCCEECCCCCCC
>Mature Secondary Structure 
PEWLRRPIGKASELSTVQQIVKRHNIHTICEEGRCPNRGECYAQKTATFLLMGPVCTRA
CHHHHHHCCCHHHHHHHHHHHHHCCHHHHHHCCCCCCCCCHHHHHHHHHEEECHHHHHH
CSFCQVDKGHTPQPLNEQEPQKVADSVRLLGLKYVVLTSVARDDLPDQGAGWFAATMAAI
HHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHH
RTVSPHTDIEVLTPDFRGDLNLIATVVAARPVCYNHNLETVRRLQGPVRRGAKYERSLQV
HHCCCCCCEEEECCCCCCCHHHHHHHHHHCCHHCCCCHHHHHHHHHHHHHCHHHHHHHHH
LATVKELAPKNDVIPSIATKSGLMLGLGETETEIIETMEDLRSVGCDRITLGQYMRPSLN
HHHHHHHCCCCCCCCCHHCCCCCEEECCCHHHHHHHHHHHHHHCCCCEEEHHHHHCCCCC
HLPVQKYWHPEEFDRLGEIARSLGFSHVRSGPLVRSSYHAGE
CCCCHHCCCHHHHHHHHHHHHHHCHHHHCCCCCEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12240834 [H]