Click here to switch to the map view.

The map label for this gene is recF

Identifier: 158334838

GI number: 158334838

Start: 1652576

End: 1653700

Strand: Reverse

Name: recF

Synonym: AM1_1675

Alternate gene names: 158334838

Gene position: 1653700-1652576 (Counterclockwise)

Preceding gene: 158334839

Following gene: 158334837

Centisome position: 25.43

GC content: 52.18

Gene sequence:

>1125_bases
GTGTATCTCCAGCAGCTCCACCTGATCCAATTTCGCAACTATGTCGCCCAGCAGGTGGAGTTCTCTGCTCCCAAAACGAT
TTTGGTGGGGCCGAATGCCCAGGGAAAGTCCAACCTCTTAGAAGCGGTGGAGCTGCTTTCAACCTTAAAATCTCATCGAG
TCAGCCGCGATCGCGACTTGGTTAAAGACGGCGAATCCCTCAGCCAGGTAACGGCGACCCTCCAACGGGAAAGTGGCCCT
CTCGATTTGAGTTTGACCTTGCGGGCTAATGGCCGTCGCACGGTTTCCGTGAACAGTGAAACGATTCGTCGCCAGCTCGA
TTTCCTTGGACACCTCAATATTGTCCAGTTTTCCAGTCTCGACATGGATTTGGTGCGGGGTGGCCCCGGAGAGCGACGGA
ATTGGCTAGATGCAGTGCTGGTGCAGTTGGAACCTGTCTATGCCCATTTGCTGCAGCAATATCAGCAGGTCCTTAAGCAG
CGTAATGCTTATCTGAAGCACCATCGTGCTGATGATGCTCCTCAGCTTGACCCCCAGCAACTTGTTTTGTGGAATCAGCA
GCTGGCAGCGAGTGGTTCACGGGTGATTCAGAGGCGTCAGCGGATGTTGATGCGGTTAGTCCCCCTAGCAGGCCACTGGC
ACCAAACCATTAGTGGCCATCAAGAACAGCTAGAAATTCTCTACACTCCTAACGTTAGCTATGACCCCCAATTTCCTGAG
CAGCTCTATCCGCAGTTCCTTAGCCAACTGGAGGAAAAGTCGATGCTGGAGCAATTGCAAGGTCTATCCCTGGTTGGTCC
CCATCGAGATGAAGTGACGTTACTTATTAATGGCACTCCAGCCCGTCAGTATGGGTCGCAAGGCCAGCAGCGCACATTAG
TTTTGGCTCTGAAGTTAGCGGAGCTGAAGCTGATTGAGGAAGTGGTGGGTGAAGCCCCCCTACTGCTCCTAGATGATGTG
CTAGCGGAGCTGGATCTGAATCGGCAAAACCAGCTTTTAGATGCGATTCAGACTCGCTTTCAAACCCTGATTACGACAAC
CCATCTAGGTGCTTTTGATGCTAAATGGCTAGACTCTGCGCAAATCTTGACGGTAAAAGCAGGGCAGGTCCAGTTTTCAA
CCTAA

Upstream 100 bases:

>100_bases
AGAGCGTCTAGAACTAGCCATGGCTTGCTTCCAGTGCCTACCCACCATCGTCACCCTTGACCTGATGCATTTTGACAATA
TTTTTGAGCATTAAGCCCTT

Downstream 100 bases:

>100_bases
CGCTATTCTGTAACTAGCACTGTTAGAACTTTGCGATATGGTTCAAGTTCCCTCTAAATCCCTGACTCTGGGAGAATTTC
TGAGATTACCGGAGACCAAA

Product: recombination protein F

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 374; Mature: 374

Protein sequence:

>374_residues
MYLQQLHLIQFRNYVAQQVEFSAPKTILVGPNAQGKSNLLEAVELLSTLKSHRVSRDRDLVKDGESLSQVTATLQRESGP
LDLSLTLRANGRRTVSVNSETIRRQLDFLGHLNIVQFSSLDMDLVRGGPGERRNWLDAVLVQLEPVYAHLLQQYQQVLKQ
RNAYLKHHRADDAPQLDPQQLVLWNQQLAASGSRVIQRRQRMLMRLVPLAGHWHQTISGHQEQLEILYTPNVSYDPQFPE
QLYPQFLSQLEEKSMLEQLQGLSLVGPHRDEVTLLINGTPARQYGSQGQQRTLVLALKLAELKLIEEVVGEAPLLLLDDV
LAELDLNRQNQLLDAIQTRFQTLITTTHLGAFDAKWLDSAQILTVKAGQVQFST

Sequences:

>Translated_374_residues
MYLQQLHLIQFRNYVAQQVEFSAPKTILVGPNAQGKSNLLEAVELLSTLKSHRVSRDRDLVKDGESLSQVTATLQRESGP
LDLSLTLRANGRRTVSVNSETIRRQLDFLGHLNIVQFSSLDMDLVRGGPGERRNWLDAVLVQLEPVYAHLLQQYQQVLKQ
RNAYLKHHRADDAPQLDPQQLVLWNQQLAASGSRVIQRRQRMLMRLVPLAGHWHQTISGHQEQLEILYTPNVSYDPQFPE
QLYPQFLSQLEEKSMLEQLQGLSLVGPHRDEVTLLINGTPARQYGSQGQQRTLVLALKLAELKLIEEVVGEAPLLLLDDV
LAELDLNRQNQLLDAIQTRFQTLITTTHLGAFDAKWLDSAQILTVKAGQVQFST
>Mature_374_residues
MYLQQLHLIQFRNYVAQQVEFSAPKTILVGPNAQGKSNLLEAVELLSTLKSHRVSRDRDLVKDGESLSQVTATLQRESGP
LDLSLTLRANGRRTVSVNSETIRRQLDFLGHLNIVQFSSLDMDLVRGGPGERRNWLDAVLVQLEPVYAHLLQQYQQVLKQ
RNAYLKHHRADDAPQLDPQQLVLWNQQLAASGSRVIQRRQRMLMRLVPLAGHWHQTISGHQEQLEILYTPNVSYDPQFPE
QLYPQFLSQLEEKSMLEQLQGLSLVGPHRDEVTLLINGTPARQYGSQGQQRTLVLALKLAELKLIEEVVGEAPLLLLDDV
LAELDLNRQNQLLDAIQTRFQTLITTTHLGAFDAKWLDSAQILTVKAGQVQFST

Specific function: The recF protein is involved in DNA metabolism; it is required for DNA replication and normal SOS inducibility. RecF binds preferentially to single-stranded, linear DNA. It also seems to bind ATP

COG id: COG1195

COG function: function code L; Recombinational DNA repair ATPase (RecF pathway)

Gene ontology:
GO:0000166: DNA replication and repair protein recF
GO:0003677: DNA replication and repair protein recF
GO:0003697: DNA replication and repair protein recF
GO:0005524: DNA replication and repair protein recF
GO:0005694: DNA replication and repair protein recF
GO:0005737: DNA replication and repair protein recF
GO:0006260: DNA replication and repair protein recF
GO:0006281: DNA replication and repair protein recF
GO:0006974: DNA replication and repair protein recF
GO:0009432: DNA replication and repair protein recF

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the recF family

Homologues:

Organism=Escherichia coli, GI1790135, Length=375, Percent_Identity=24.8, Blast_Score=83, Evalue=3e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RECF_ACAM1 (B0CB57)

Other databases:

- EMBL:   CP000828
- RefSeq:   YP_001516010.1
- ProteinModelPortal:   B0CB57
- SMR:   B0CB57
- GeneID:   5680491
- GenomeReviews:   CP000828_GR
- KEGG:   amr:AM1_1675
- HOGENOM:   HBG645124
- OMA:   GQQRTLV
- ProtClustDB:   PRK00064
- BioCyc:   AMAR329726:AM1_1675-MONOMER
- GO:   GO:0005694
- GO:   GO:0005737
- HAMAP:   MF_00365
- InterPro:   IPR001238
- InterPro:   IPR018078
- InterPro:   IPR003395
- TIGRFAMs:   TIGR00611

Pfam domain/function: PF02463 SMC_N

EC number: NA

Molecular weight: Translated: 42415; Mature: 42415

Theoretical pI: Translated: 6.93; Mature: 6.93

Prosite motif: PS00617 RECF_1; PS00618 RECF_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYLQQLHLIQFRNYVAQQVEFSAPKTILVGPNAQGKSNLLEAVELLSTLKSHRVSRDRDL
CCHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCHHH
VKDGESLSQVTATLQRESGPLDLSLTLRANGRRTVSVNSETIRRQLDFLGHLNIVQFSSL
HHCCHHHHHHHHHHHHCCCCEEEEEEEEECCCEEEEECHHHHHHHHHHHHCCCEEEECCC
DMDLVRGGPGERRNWLDAVLVQLEPVYAHLLQQYQQVLKQRNAYLKHHRADDAPQLDPQQ
CHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHH
LVLWNQQLAASGSRVIQRRQRMLMRLVPLAGHWHQTISGHQEQLEILYTPNVSYDPQFPE
HHHHHHHHHHCHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCEEEEECCCCCCCCCCCH
QLYPQFLSQLEEKSMLEQLQGLSLVGPHRDEVTLLINGTPARQYGSQGQQRTLVLALKLA
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCHHHHCCCCCCEEEHHHHHHH
ELKLIEEVVGEAPLLLLDDVLAELDLNRQNQLLDAIQTRFQTLITTTHLGAFDAKWLDSA
HHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCC
QILTVKAGQVQFST
EEEEEEECEEEECC
>Mature Secondary Structure
MYLQQLHLIQFRNYVAQQVEFSAPKTILVGPNAQGKSNLLEAVELLSTLKSHRVSRDRDL
CCHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCHHH
VKDGESLSQVTATLQRESGPLDLSLTLRANGRRTVSVNSETIRRQLDFLGHLNIVQFSSL
HHCCHHHHHHHHHHHHCCCCEEEEEEEEECCCEEEEECHHHHHHHHHHHHCCCEEEECCC
DMDLVRGGPGERRNWLDAVLVQLEPVYAHLLQQYQQVLKQRNAYLKHHRADDAPQLDPQQ
CHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHH
LVLWNQQLAASGSRVIQRRQRMLMRLVPLAGHWHQTISGHQEQLEILYTPNVSYDPQFPE
HHHHHHHHHHCHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCEEEEECCCCCCCCCCCH
QLYPQFLSQLEEKSMLEQLQGLSLVGPHRDEVTLLINGTPARQYGSQGQQRTLVLALKLA
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCHHHHCCCCCCEEEHHHHHHH
ELKLIEEVVGEAPLLLLDDVLAELDLNRQNQLLDAIQTRFQTLITTTHLGAFDAKWLDSA
HHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCC
QILTVKAGQVQFST
EEEEEEECEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA