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The map label for this gene is yafJ [H]

Identifier: 158334831

GI number: 158334831

Start: 1646415

End: 1647218

Strand: Reverse

Name: yafJ [H]

Synonym: AM1_1667

Alternate gene names: 158334831

Gene position: 1647218-1646415 (Counterclockwise)

Preceding gene: 158334834

Following gene: 158334829

Centisome position: 25.33

GC content: 47.89

Gene sequence:

>804_bases
ATGTGTCAGTTATTGGGGATGAACTGTAATGGGCCAACGGATATTTGTTTCTCCTTTGAAGGGTTCTGCGCCCGAGGTGG
CAAAACGGACGATCATAAAGATGGGTGGGGAATTGCCTTTTTTGAAGGAGCAGCCTGTCGAGTTTTTCTAGATGACAAGC
CTTCTGCCACCTCTGCCATTGCAAAACTGATTCGGGAATACCCGATTAAGTCCACCAATGTTGTCGCTCATATTCGCAAA
GCCACCATTGGTCCAGTGGGCTTAGAAAATTGTCATCCTTTTACGCGGGAACTGTGGGGGCGACATTGGGTATTTGCTCA
TAATGGGGACCTACCCACTCTGAAATCTGGAAGTTTAGGCACCTATCGCTCCGTGGGCCAAACGGATAGTGAACGAGCCT
TCTGCTTGATTATGAATCAGCTCAGAGATAAATTTCCCCAGCAACAACCGCCCATGGAGGAGCTGTTCCCTGTGCTGCAA
GAGATGACCCAGGCCATTGCCAGTCATGGGATTTTCAACTATTTGCTCTCAGATGGAAAACACCTGTTTGTGCACTGTTC
CACCAAACTTTGCTATATCGTTCGTCAGTCTCCCTTTGCGGCAGCACACCTAATTGATGAAGATTTGACGGTTGATTTTC
AGGAGTTGGCAACACCTTGCGATCGCGTCGCCATTATCGCCACCACTTGTCTAACGGATAACGAAACCTGGACTACCATC
CAACCCGGTGAACTGCTTGTCTTTCAGGATGGGTTACCGCTGATTTTTCAAGATGATTGGGCTTGTAAAGTCAGTCGTTA
TTGA

Upstream 100 bases:

>100_bases
TTTCTTGCGCGCAAGATTGTGGGAATTGGCATGGTATTTTGTTGCGGCCTCTTTCCCTTTCCACCTACAATCGACGGCGT
AGAACTGCTTGCGATCGCTC

Downstream 100 bases:

>100_bases
GGGGAAAACACCAAGCGTATTGCTGAGATCAACTGATTAAAACCAGAGAGTGTAATAGGTTGGCGGTGCTCCACACATAA
GACAGCAGGTTGAAACGGCA

Product: glutamine amidotransferase YafJ

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 267; Mature: 267

Protein sequence:

>267_residues
MCQLLGMNCNGPTDICFSFEGFCARGGKTDDHKDGWGIAFFEGAACRVFLDDKPSATSAIAKLIREYPIKSTNVVAHIRK
ATIGPVGLENCHPFTRELWGRHWVFAHNGDLPTLKSGSLGTYRSVGQTDSERAFCLIMNQLRDKFPQQQPPMEELFPVLQ
EMTQAIASHGIFNYLLSDGKHLFVHCSTKLCYIVRQSPFAAAHLIDEDLTVDFQELATPCDRVAIIATTCLTDNETWTTI
QPGELLVFQDGLPLIFQDDWACKVSRY

Sequences:

>Translated_267_residues
MCQLLGMNCNGPTDICFSFEGFCARGGKTDDHKDGWGIAFFEGAACRVFLDDKPSATSAIAKLIREYPIKSTNVVAHIRK
ATIGPVGLENCHPFTRELWGRHWVFAHNGDLPTLKSGSLGTYRSVGQTDSERAFCLIMNQLRDKFPQQQPPMEELFPVLQ
EMTQAIASHGIFNYLLSDGKHLFVHCSTKLCYIVRQSPFAAAHLIDEDLTVDFQELATPCDRVAIIATTCLTDNETWTTI
QPGELLVFQDGLPLIFQDDWACKVSRY
>Mature_267_residues
MCQLLGMNCNGPTDICFSFEGFCARGGKTDDHKDGWGIAFFEGAACRVFLDDKPSATSAIAKLIREYPIKSTNVVAHIRK
ATIGPVGLENCHPFTRELWGRHWVFAHNGDLPTLKSGSLGTYRSVGQTDSERAFCLIMNQLRDKFPQQQPPMEELFPVLQ
EMTQAIASHGIFNYLLSDGKHLFVHCSTKLCYIVRQSPFAAAHLIDEDLTVDFQELATPCDRVAIIATTCLTDNETWTTI
QPGELLVFQDGLPLIFQDDWACKVSRY

Specific function: Unknown

COG id: COG0121

COG function: function code R; Predicted glutamine amidotransferase

Gene ontology:
GO:0006541: Glutamine amidotransferase YafJ, putative
GO:0008152: Glutamine amidotransferase YafJ, putative
GO:0016740: Glutamine amidotransferase YafJ, putative

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Escherichia coli, GI1786417, Length=248, Percent_Identity=52.8225806451613, Blast_Score=287, Evalue=5e-79,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000583
- InterPro:   IPR017932 [H]

Pfam domain/function: PF00310 GATase_2 [H]

EC number: NA

Molecular weight: Translated: 29854; Mature: 29854

Theoretical pI: Translated: 5.79; Mature: 5.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

4.5 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
6.4 %Cys+Met (Translated Protein)
4.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
6.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCQLLGMNCNGPTDICFSFEGFCARGGKTDDHKDGWGIAFFEGAACRVFLDDKPSATSAI
CCEEEECCCCCCCCEEEECCCHHCCCCCCCCCCCCCEEEEECCCEEEEEECCCCCHHHHH
AKLIREYPIKSTNVVAHIRKATIGPVGLENCHPFTRELWGRHWVFAHNGDLPTLKSGSLG
HHHHHHCCCCCCHHHHHHHHHCCCCCCCCCCCHHHHHHCCCEEEEEECCCCCCCCCCCCC
TYRSVGQTDSERAFCLIMNQLRDKFPQQQPPMEELFPVLQEMTQAIASHGIFNYLLSDGK
CCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
HLFVHCSTKLCYIVRQSPFAAAHLIDEDLTVDFQELATPCDRVAIIATTCLTDNETWTTI
EEEEEECCEEEEEEECCCCHHHHHHCCCCCCCHHHHCCCHHHEEEEEEEEECCCCEEEEE
QPGELLVFQDGLPLIFQDDWACKVSRY
CCCEEEEEECCCCEEEECCCEEEECCC
>Mature Secondary Structure
MCQLLGMNCNGPTDICFSFEGFCARGGKTDDHKDGWGIAFFEGAACRVFLDDKPSATSAI
CCEEEECCCCCCCCEEEECCCHHCCCCCCCCCCCCCEEEEECCCEEEEEECCCCCHHHHH
AKLIREYPIKSTNVVAHIRKATIGPVGLENCHPFTRELWGRHWVFAHNGDLPTLKSGSLG
HHHHHHCCCCCCHHHHHHHHHCCCCCCCCCCCHHHHHHCCCEEEEEECCCCCCCCCCCCC
TYRSVGQTDSERAFCLIMNQLRDKFPQQQPPMEELFPVLQEMTQAIASHGIFNYLLSDGK
CCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
HLFVHCSTKLCYIVRQSPFAAAHLIDEDLTVDFQELATPCDRVAIIATTCLTDNETWTTI
EEEEEECCEEEEEEECCCCHHHHHHCCCCCCCHHHHCCCHHHEEEEEEEEECCCCEEEEE
QPGELLVFQDGLPLIFQDDWACKVSRY
CCCEEEEEECCCCEEEECCCEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7596361; 9278503 [H]