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The map label for this gene is acnB [H]

Identifier: 158334795

GI number: 158334795

Start: 1597762

End: 1600380

Strand: Reverse

Name: acnB [H]

Synonym: AM1_1630

Alternate gene names: 158334795

Gene position: 1600380-1597762 (Counterclockwise)

Preceding gene: 158334796

Following gene: 158334792

Centisome position: 24.61

GC content: 51.62

Gene sequence:

>2619_bases
ATGGGATCTGGTTTGGAAATGCTGACATCTTACCGCCAACATGTTCAGGAACGGGCTGCTTTAGGCATTCCGCCGCTACC
GTTGACGGCTGAGCAGACGGCTGATCTGTGTGAGCTGCTCAAGTCTCCGCCTGCAGGGGAAGAAGATGCGTTGATGGCAT
TGCTGCGAGATCGCATCCCGCCCGGTGTCGATCAGGCGGCCTATGTGAAGGCTTCGTTTTTGAGTGCGATCGCAAACCAC
GAAACCACCAGTCCCCTCATCACCCCCATCGCTGCGGTTGAACTCCTCGGTACGATGATGGGCGGCTACAACGTGCAGTC
TTTAGTGGACTTACTTAAATCAGACAATGCTGAACTCGCCACCGCTGCCGCCACGGCCCTCAAGCATACGGTTTTGGTCT
ACGATGCCTACCATGATGTGATAGAGCTCGCCCAAACCAATTCTTATGCCCAGTCCGTGGTTGATTCCTGGGTGGCAGCA
GAATGGTTTACCAGCAAATCCAAGTTGCCTGAATCCATTACCGTTACCGTGTTTAAAGTACCGGGGGAAACGAATACGGA
CGATTTGTCTCCCGCCCCCCATGCCACCACACGCCCTGATATCCCGCTGCATGCGCTAGCCATGTTGGAAACCCGAATGC
CAGACGGGCTAGAAACTTTGGCTCAACTCAAGCAAAAAGGTCATCCCGTCGCCTATGTTGGAGATGTGGTTGGTACCGGA
TCATCCCGGAAATCAGCCATTAACTCGGTCCTCTGGCATATTGGCGATGATATTCCCTTTGTTCCCAACAAGCGAGCTGG
CGGCTATATCTTAGGGGGCAATATTGCACCTATTTTCTTCAACACGGCGGAAGATTCAGGCGCATTGCCCATTGAATGTG
ATGTCTCTGGTCTAGATACTGGGATGGTGATTACTATCTATCCCTTCAAAGGCGAAATCACAGATGAAGCAGGTACCGTC
CTCACCACTTTTACCCTCAAACCTGAAACGATTACCGACGAGGTCCAAGCGGGAGGTCGGATACCTTTACTGATTGGTCG
CTCTCTAACGGACAAAATTCGCATTGAGCTGGGAATGGAGCCAAGTGATGTCTTTACCCGACCCACTCCACCCCCTGATT
CTGGCAAAGGCTTTACCCTCGCCCAAAAAATGGTGGGCAAGGCTTGTGGTCTACCAGGCGTTCGCCCCGGCATGTCCTGC
GAACCCTTAATGACCACTGTGGGATCGCAAGACACCACAGGCCCCATGACCCGTGATGAAATGAAAGAGCTAGCATGTCT
AGGCTTTAGCTCTGATCTGGTCATGCAAAGCTTCTGCCATACAGCGGCCTATCCCAAGCCCGTTGATATTGAAACCCATA
CCCATCTGCCTGACTTCTTCGCTTCTCGGGGTGGCGTCGCCCTGCGCCCTGGAGATGGCATTATCCACTCTTGGCTCAAT
CGGATGTTGCTGCCTGACACCGTGGGTACAGGAGGCGATTCTCACACCCGCTTTCCCTTAGGCATTTCCTTCCCTGCTGG
TTCTGGATTGGTGGCCTTTGCGGCAGCGATTGGGGCTATGCCCTTAGATATGCCAGAGTCGGTCTTGGTCAAATTTACGG
GTGATCTACAGCCTGGAATTACCCTGCGGGATATCGTCAATGCTATTCCCTATGTCGCTATCCAAAAGGGATTGCTGACC
GTTGAAAAACAGAATAAGAAAAATATCTACTCTGGCAGAGTCCTAGAGATGGAAGGATTGCCGGATCTAAAACTAGAGCA
AGCCTTTGAATTAACAGATGCATCGGCAGAGCGATCAGCCTCGGGCTGCACAATTAAGCTGAGTGAAGAGACGGTAGCCG
AATATTTGCGCTCTAATATTGCCCTACTTAAGAATATGGCAGCCCGAGGCTATCAGGATGCCCGGACGATTCTTCGCCGT
GTGGCCAAGATGGAAGCATGGCTGGCAAATCCAACCTTAATGTCAGCAGATGCCGATGCAGAGTATGCCGAAGTGGTGGA
CGTGAACCTCAATGAGATTCGTGAACCGCTGGTGGCTGCGCCTAACGATCCTGACAATATTAAAACCCTATCTGAAGTGG
TGAATGACCCCATCCATGAGGTGTTTATTGGTTCCTGCATGACCAATATTGGTCACTACCGGGCCGCAGCTAAGGTCTTG
GAAGGAGCAGGACCCACAAAAGCGCGGCTCTGGATCTGTCCACCCACTCGTATGGATGAAAAGCAACTCCGAGAAGAGGG
CTATTACGGTATTTTTGCGGCAGCTGGAGCCCGAACCGAGATGCCAGGTTGTTCCCTCTGTATGGGAAATCAAGCCCGTG
TGGAAGATGGGGTAACGGTGTTCTCCACCTCAACTCGTAACTTTAATAATCGGATGGGTAAAGGGGCGCAGGTTTATTTA
GGTTCAGCAGAATTGGCTGCCGCTTGTGCTCTCCTCGGCCGTATTCCCACCCCTGAGGAATACCAGGATATCGTCGCTAA
AAAGATTGATCCGTTTGCGGATGATCTGTACCGCTATCTCAATTTCGATCAGATTGATGGCTTTATTGATGAAGGTCGCG
TGATTCCTTTGGAGGAGTTACCTAAAATTGAAGATATTTTAGGGATGCCCGTGAGCTGA

Upstream 100 bases:

>100_bases
GAATGGACGAAATGACCTGGCAGTATGACGCTACACATCAGACTCTGACAATCCAGCGATATATTAGGTATAGGACTCTG
CTATTTATTGAGAACGCTTC

Downstream 100 bases:

>100_bases
AGAATAACAGATAAGGCACAGATTCTTTAGAACTCACTGAAGCAGGAACGGTTACGGCCCTGAGCTTTGGCTTGGTACAG
TGCTTTATCTGCCTGTTCGA

Product: bifunctional aconitate hydratase 2/2-methylisocitrate dehydratase

Products: NA

Alternate protein names: Aconitase 2; 2-methylisocitrate dehydratase; Citrate hydro-lyase 2 [H]

Number of amino acids: Translated: 872; Mature: 871

Protein sequence:

>872_residues
MGSGLEMLTSYRQHVQERAALGIPPLPLTAEQTADLCELLKSPPAGEEDALMALLRDRIPPGVDQAAYVKASFLSAIANH
ETTSPLITPIAAVELLGTMMGGYNVQSLVDLLKSDNAELATAAATALKHTVLVYDAYHDVIELAQTNSYAQSVVDSWVAA
EWFTSKSKLPESITVTVFKVPGETNTDDLSPAPHATTRPDIPLHALAMLETRMPDGLETLAQLKQKGHPVAYVGDVVGTG
SSRKSAINSVLWHIGDDIPFVPNKRAGGYILGGNIAPIFFNTAEDSGALPIECDVSGLDTGMVITIYPFKGEITDEAGTV
LTTFTLKPETITDEVQAGGRIPLLIGRSLTDKIRIELGMEPSDVFTRPTPPPDSGKGFTLAQKMVGKACGLPGVRPGMSC
EPLMTTVGSQDTTGPMTRDEMKELACLGFSSDLVMQSFCHTAAYPKPVDIETHTHLPDFFASRGGVALRPGDGIIHSWLN
RMLLPDTVGTGGDSHTRFPLGISFPAGSGLVAFAAAIGAMPLDMPESVLVKFTGDLQPGITLRDIVNAIPYVAIQKGLLT
VEKQNKKNIYSGRVLEMEGLPDLKLEQAFELTDASAERSASGCTIKLSEETVAEYLRSNIALLKNMAARGYQDARTILRR
VAKMEAWLANPTLMSADADAEYAEVVDVNLNEIREPLVAAPNDPDNIKTLSEVVNDPIHEVFIGSCMTNIGHYRAAAKVL
EGAGPTKARLWICPPTRMDEKQLREEGYYGIFAAAGARTEMPGCSLCMGNQARVEDGVTVFSTSTRNFNNRMGKGAQVYL
GSAELAAACALLGRIPTPEEYQDIVAKKIDPFADDLYRYLNFDQIDGFIDEGRVIPLEELPKIEDILGMPVS

Sequences:

>Translated_872_residues
MGSGLEMLTSYRQHVQERAALGIPPLPLTAEQTADLCELLKSPPAGEEDALMALLRDRIPPGVDQAAYVKASFLSAIANH
ETTSPLITPIAAVELLGTMMGGYNVQSLVDLLKSDNAELATAAATALKHTVLVYDAYHDVIELAQTNSYAQSVVDSWVAA
EWFTSKSKLPESITVTVFKVPGETNTDDLSPAPHATTRPDIPLHALAMLETRMPDGLETLAQLKQKGHPVAYVGDVVGTG
SSRKSAINSVLWHIGDDIPFVPNKRAGGYILGGNIAPIFFNTAEDSGALPIECDVSGLDTGMVITIYPFKGEITDEAGTV
LTTFTLKPETITDEVQAGGRIPLLIGRSLTDKIRIELGMEPSDVFTRPTPPPDSGKGFTLAQKMVGKACGLPGVRPGMSC
EPLMTTVGSQDTTGPMTRDEMKELACLGFSSDLVMQSFCHTAAYPKPVDIETHTHLPDFFASRGGVALRPGDGIIHSWLN
RMLLPDTVGTGGDSHTRFPLGISFPAGSGLVAFAAAIGAMPLDMPESVLVKFTGDLQPGITLRDIVNAIPYVAIQKGLLT
VEKQNKKNIYSGRVLEMEGLPDLKLEQAFELTDASAERSASGCTIKLSEETVAEYLRSNIALLKNMAARGYQDARTILRR
VAKMEAWLANPTLMSADADAEYAEVVDVNLNEIREPLVAAPNDPDNIKTLSEVVNDPIHEVFIGSCMTNIGHYRAAAKVL
EGAGPTKARLWICPPTRMDEKQLREEGYYGIFAAAGARTEMPGCSLCMGNQARVEDGVTVFSTSTRNFNNRMGKGAQVYL
GSAELAAACALLGRIPTPEEYQDIVAKKIDPFADDLYRYLNFDQIDGFIDEGRVIPLEELPKIEDILGMPVS
>Mature_871_residues
GSGLEMLTSYRQHVQERAALGIPPLPLTAEQTADLCELLKSPPAGEEDALMALLRDRIPPGVDQAAYVKASFLSAIANHE
TTSPLITPIAAVELLGTMMGGYNVQSLVDLLKSDNAELATAAATALKHTVLVYDAYHDVIELAQTNSYAQSVVDSWVAAE
WFTSKSKLPESITVTVFKVPGETNTDDLSPAPHATTRPDIPLHALAMLETRMPDGLETLAQLKQKGHPVAYVGDVVGTGS
SRKSAINSVLWHIGDDIPFVPNKRAGGYILGGNIAPIFFNTAEDSGALPIECDVSGLDTGMVITIYPFKGEITDEAGTVL
TTFTLKPETITDEVQAGGRIPLLIGRSLTDKIRIELGMEPSDVFTRPTPPPDSGKGFTLAQKMVGKACGLPGVRPGMSCE
PLMTTVGSQDTTGPMTRDEMKELACLGFSSDLVMQSFCHTAAYPKPVDIETHTHLPDFFASRGGVALRPGDGIIHSWLNR
MLLPDTVGTGGDSHTRFPLGISFPAGSGLVAFAAAIGAMPLDMPESVLVKFTGDLQPGITLRDIVNAIPYVAIQKGLLTV
EKQNKKNIYSGRVLEMEGLPDLKLEQAFELTDASAERSASGCTIKLSEETVAEYLRSNIALLKNMAARGYQDARTILRRV
AKMEAWLANPTLMSADADAEYAEVVDVNLNEIREPLVAAPNDPDNIKTLSEVVNDPIHEVFIGSCMTNIGHYRAAAKVLE
GAGPTKARLWICPPTRMDEKQLREEGYYGIFAAAGARTEMPGCSLCMGNQARVEDGVTVFSTSTRNFNNRMGKGAQVYLG
SAELAAACALLGRIPTPEEYQDIVAKKIDPFADDLYRYLNFDQIDGFIDEGRVIPLEELPKIEDILGMPVS

Specific function: Catalyzes the isomerization of citrate to isocitrate via cis-aconitate as well as the dehydration of 2-methylisocitrate to cis-2-methylaconitate [H]

COG id: COG1049

COG function: function code C; Aconitase B

Gene ontology:
GO:0003994: Aconitate hydratase 2
GO:0006099: Aconitate hydratase 2
GO:0008152: Aconitate hydratase 2
GO:0016829: Aconitate hydratase 2
GO:0051539: Aconitate hydratase 2
GO:0052632: Aconitate hydratase 2
GO:0052633: Aconitate hydratase 2

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aconitase/IPM isomerase family [H]

Homologues:

Organism=Escherichia coli, GI2367097, Length=858, Percent_Identity=66.5501165501165, Blast_Score=1124, Evalue=0.0,
Organism=Escherichia coli, GI1786259, Length=376, Percent_Identity=25.7978723404255, Blast_Score=78, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015931
- InterPro:   IPR015937
- InterPro:   IPR001030
- InterPro:   IPR015928
- InterPro:   IPR018136
- InterPro:   IPR004406
- InterPro:   IPR015930
- InterPro:   IPR015933
- InterPro:   IPR015929 [H]

Pfam domain/function: PF00330 Aconitase; PF06434 Aconitase_2_N; PF11791 Aconitase_B_N [H]

EC number: =4.2.1.3; =4.2.1.99 [H]

Molecular weight: Translated: 93836; Mature: 93705

Theoretical pI: Translated: 4.61; Mature: 4.61

Prosite motif: PS00450 ACONITASE_1 ; PS01244 ACONITASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGSGLEMLTSYRQHVQERAALGIPPLPLTAEQTADLCELLKSPPAGEEDALMALLRDRIP
CCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCC
PGVDQAAYVKASFLSAIANHETTSPLITPIAAVELLGTMMGGYNVQSLVDLLKSDNAELA
CCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCHHH
TAAATALKHTVLVYDAYHDVIELAQTNSYAQSVVDSWVAAEWFTSKSKLPESITVTVFKV
HHHHHHHHHEEEEEHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEC
PGETNTDDLSPAPHATTRPDIPLHALAMLETRMPDGLETLAQLKQKGHPVAYVGDVVGTG
CCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHCCCCEEEECCCCCCC
SSRKSAINSVLWHIGDDIPFVPNKRAGGYILGGNIAPIFFNTAEDSGALPIECDVSGLDT
CHHHHHHHHHHHHHCCCCCCCCCCCCCCEEECCCEEEEEEECCCCCCCCEEEECCCCCCC
GMVITIYPFKGEITDEAGTVLTTFTLKPETITDEVQAGGRIPLLIGRSLTDKIRIELGME
CEEEEEEECCCCCCCCCCCEEEEEEECCCHHHHHHHCCCCEEEEECCCCCCEEEEEECCC
PSDVFTRPTPPPDSGKGFTLAQKMVGKACGLPGVRPGMSCEPLMTTVGSQDTTGPMTRDE
HHHCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCHHHHHCCCCCCCCCCCHHH
MKELACLGFSSDLVMQSFCHTAAYPKPVDIETHTHLPDFFASRGGVALRPGDGIIHSWLN
HHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHCCCCEEECCCCCHHHHHHH
RMLLPDTVGTGGDSHTRFPLGISFPAGSGLVAFAAAIGAMPLDMPESVLVKFTGDLQPGI
HHCCCCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHCCCCCCCCHHHEEEEECCCCCCC
TLRDIVNAIPYVAIQKGLLTVEKQNKKNIYSGRVLEMEGLPDLKLEQAFELTDASAERSA
CHHHHHHHHHHHHHHHCCEEEECCCCCCCCCCEEEEECCCCCCHHHHHHHHCCCHHHCCC
SGCTIKLSEETVAEYLRSNIALLKNMAARGYQDARTILRRVAKMEAWLANPTLMSADADA
CCCEEEECHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCEEECCCCC
EYAEVVDVNLNEIREPLVAAPNDPDNIKTLSEVVNDPIHEVFIGSCMTNIGHYRAAAKVL
CCHHEEECCHHHHHHHHEECCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHH
EGAGPTKARLWICPPTRMDEKQLREEGYYGIFAAAGARTEMPGCSLCMGNQARVEDGVTV
HCCCCCCEEEEECCCCCCCHHHHHHCCCEEEEEECCCCCCCCCCCEECCCCCCCCCCEEE
FSTSTRNFNNRMGKGAQVYLGSAELAAACALLGRIPTPEEYQDIVAKKIDPFADDLYRYL
EEECCCCHHHHCCCCCEEEECCHHHHHHHHHHCCCCCCHHHHHHHHHHCCHHHHHHHHHH
NFDQIDGFIDEGRVIPLEELPKIEDILGMPVS
CHHHHCCHHCCCCEECHHHCCCHHHHHCCCCC
>Mature Secondary Structure 
GSGLEMLTSYRQHVQERAALGIPPLPLTAEQTADLCELLKSPPAGEEDALMALLRDRIP
CCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCC
PGVDQAAYVKASFLSAIANHETTSPLITPIAAVELLGTMMGGYNVQSLVDLLKSDNAELA
CCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCHHH
TAAATALKHTVLVYDAYHDVIELAQTNSYAQSVVDSWVAAEWFTSKSKLPESITVTVFKV
HHHHHHHHHEEEEEHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEC
PGETNTDDLSPAPHATTRPDIPLHALAMLETRMPDGLETLAQLKQKGHPVAYVGDVVGTG
CCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHCCCCEEEECCCCCCC
SSRKSAINSVLWHIGDDIPFVPNKRAGGYILGGNIAPIFFNTAEDSGALPIECDVSGLDT
CHHHHHHHHHHHHHCCCCCCCCCCCCCCEEECCCEEEEEEECCCCCCCCEEEECCCCCCC
GMVITIYPFKGEITDEAGTVLTTFTLKPETITDEVQAGGRIPLLIGRSLTDKIRIELGME
CEEEEEEECCCCCCCCCCCEEEEEEECCCHHHHHHHCCCCEEEEECCCCCCEEEEEECCC
PSDVFTRPTPPPDSGKGFTLAQKMVGKACGLPGVRPGMSCEPLMTTVGSQDTTGPMTRDE
HHHCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCHHHHHCCCCCCCCCCCHHH
MKELACLGFSSDLVMQSFCHTAAYPKPVDIETHTHLPDFFASRGGVALRPGDGIIHSWLN
HHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHCCCCEEECCCCCHHHHHHH
RMLLPDTVGTGGDSHTRFPLGISFPAGSGLVAFAAAIGAMPLDMPESVLVKFTGDLQPGI
HHCCCCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHCCCCCCCCHHHEEEEECCCCCCC
TLRDIVNAIPYVAIQKGLLTVEKQNKKNIYSGRVLEMEGLPDLKLEQAFELTDASAERSA
CHHHHHHHHHHHHHHHCCEEEECCCCCCCCCCEEEEECCCCCCHHHHHHHHCCCHHHCCC
SGCTIKLSEETVAEYLRSNIALLKNMAARGYQDARTILRRVAKMEAWLANPTLMSADADA
CCCEEEECHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCEEECCCCC
EYAEVVDVNLNEIREPLVAAPNDPDNIKTLSEVVNDPIHEVFIGSCMTNIGHYRAAAKVL
CCHHEEECCHHHHHHHHEECCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHH
EGAGPTKARLWICPPTRMDEKQLREEGYYGIFAAAGARTEMPGCSLCMGNQARVEDGVTV
HCCCCCCEEEEECCCCCCCHHHHHHCCCEEEEEECCCCCCCCCCCEECCCCCCCCCCEEE
FSTSTRNFNNRMGKGAQVYLGSAELAAACALLGRIPTPEEYQDIVAKKIDPFADDLYRYL
EEECCCCHHHHCCCCCEEEECCHHHHHHHHHHCCCCCCHHHHHHHHHHCCHHHHHHHHHH
NFDQIDGFIDEGRVIPLEELPKIEDILGMPVS
CHHHHCCHHCCCCEECHHHCCCHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]