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The map label for this gene is selU [H]

Identifier: 158334739

GI number: 158334739

Start: 1555259

End: 1556326

Strand: Direct

Name: selU [H]

Synonym: AM1_1573

Alternate gene names: 158334739

Gene position: 1555259-1556326 (Clockwise)

Preceding gene: 158334738

Following gene: 158334740

Centisome position: 23.91

GC content: 51.78

Gene sequence:

>1068_bases
ATGCCCCTCACCCCCAACATCACAGCCGATATTTGCCCACAACTATATAGCGAAATTATCGATGTACGATCCCCCGCTGA
ATATGCGGAAGATCACCTCCCCGGTGCCATCAATTTGCCCGTACTCACGGATCAAGAACGAGCTGAGGTGGGGACGCTAT
ATAAGCAAGTGTCGGCATTTGTGGCCCGCAAGAAGGGGGCGGCACTGGTCTCTCAAAATCTCTCTTATCACCTCAAGCAC
CACTTTGCAGATCACCCCAAGGGGTATCACCCGTTAATTTACTGCTGGCGTGGGGGACAGCGATCCCAAAGTATGGCCCT
GGTACTCACCCAGGTGGGATGGCGTACAACCCTTTTGAAAGGGGGATATAAGACCTATCGCACCTATGTCCGAGAACAGC
TAGAACAGGTGCCGTATCACTTTTCGTATCGTGTATTGTGCGGACTCACGGGCACTGCCAAAACGGAGATTTTGCAGCGT
TTGGCTCAAGCTCAGGTGCAGGTCCTGGATTTAGAAGGATTGGCCAACCATCGGGGCTCGTTATTAGGAGCAGCAGAAAC
CTCTGCCCAACCCTCTCAAAAGCAATTTGAATCGGATTTACTGTCGGCCCTCCAAACCTTCGATACCGCCCACCCGGTTT
GGATCGAGGCAGAAAGCAATAAAATCGGTGAGCGCTTTGTCCCCAAGGCTCTCTGGGACCAAATGCAAGCTGCTCCTTGT
GTCGAAGTCCAAGCCCCCATGAGCAGTCGAGTGGACTGGTTGGTGACCCACTACGAGCATTTCACCCATAATTCCGCTTT
ACTCAAAGACAAGCTCAATTTACTCCGACGCAGTTACAGCAAAGCCCAACTGCAGCACTGGCATAACCTCATCGACGAGC
AACAGTGGCCAGCATTCGTTCAGTCTCTGTTGGAAGAGCATTATGATCCGGCCTATCGCCGATCGATGAAGCGCCAATAT
ACCAATATCCAGCAGCAACATCAGCTACCTGATTTATCAGCCACCAGTTTGCAACAGTTGGTGAACCAACTCGCTACAAA
CTGCAATCAGCCTGCTACGACCGTGTGA

Upstream 100 bases:

>100_bases
CTCCGCATTGACGTCAATCAGGCCCTGGAGGAAATGGATGACCTGATTGATGAATTCAGAAAAACCTTTCCTGCTAATGC
CTAGAGACTGCTAGACCTGG

Downstream 100 bases:

>100_bases
GCAAGATCATCTGCTCTTGATGATTGAGATCAACGGTCCCCAGTAATGCACATCCTAGGATTGGACTAACACATAACAAA
ACCAACGCCTCAACCTAGCG

Product: tRNA 2-selenouridine synthase

Products: NA

Alternate protein names: Selenophosphate-dependent tRNA 2-selenouridine synthase [H]

Number of amino acids: Translated: 355; Mature: 354

Protein sequence:

>355_residues
MPLTPNITADICPQLYSEIIDVRSPAEYAEDHLPGAINLPVLTDQERAEVGTLYKQVSAFVARKKGAALVSQNLSYHLKH
HFADHPKGYHPLIYCWRGGQRSQSMALVLTQVGWRTTLLKGGYKTYRTYVREQLEQVPYHFSYRVLCGLTGTAKTEILQR
LAQAQVQVLDLEGLANHRGSLLGAAETSAQPSQKQFESDLLSALQTFDTAHPVWIEAESNKIGERFVPKALWDQMQAAPC
VEVQAPMSSRVDWLVTHYEHFTHNSALLKDKLNLLRRSYSKAQLQHWHNLIDEQQWPAFVQSLLEEHYDPAYRRSMKRQY
TNIQQQHQLPDLSATSLQQLVNQLATNCNQPATTV

Sequences:

>Translated_355_residues
MPLTPNITADICPQLYSEIIDVRSPAEYAEDHLPGAINLPVLTDQERAEVGTLYKQVSAFVARKKGAALVSQNLSYHLKH
HFADHPKGYHPLIYCWRGGQRSQSMALVLTQVGWRTTLLKGGYKTYRTYVREQLEQVPYHFSYRVLCGLTGTAKTEILQR
LAQAQVQVLDLEGLANHRGSLLGAAETSAQPSQKQFESDLLSALQTFDTAHPVWIEAESNKIGERFVPKALWDQMQAAPC
VEVQAPMSSRVDWLVTHYEHFTHNSALLKDKLNLLRRSYSKAQLQHWHNLIDEQQWPAFVQSLLEEHYDPAYRRSMKRQY
TNIQQQHQLPDLSATSLQQLVNQLATNCNQPATTV
>Mature_354_residues
PLTPNITADICPQLYSEIIDVRSPAEYAEDHLPGAINLPVLTDQERAEVGTLYKQVSAFVARKKGAALVSQNLSYHLKHH
FADHPKGYHPLIYCWRGGQRSQSMALVLTQVGWRTTLLKGGYKTYRTYVREQLEQVPYHFSYRVLCGLTGTAKTEILQRL
AQAQVQVLDLEGLANHRGSLLGAAETSAQPSQKQFESDLLSALQTFDTAHPVWIEAESNKIGERFVPKALWDQMQAAPCV
EVQAPMSSRVDWLVTHYEHFTHNSALLKDKLNLLRRSYSKAQLQHWHNLIDEQQWPAFVQSLLEEHYDPAYRRSMKRQYT
NIQQQHQLPDLSATSLQQLVNQLATNCNQPATTV

Specific function: Catalyzes the transfer of selenium from selenophosphate for conversion of 2-thiouridine to 2-selenouridine at the wobble position in tRNA [H]

COG id: COG2603

COG function: function code R; Predicted ATPase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 rhodanese domain [H]

Homologues:

Organism=Escherichia coli, GI1786712, Length=343, Percent_Identity=31.1953352769679, Blast_Score=119, Evalue=3e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001763
- InterPro:   IPR017582 [H]

Pfam domain/function: PF00581 Rhodanese [H]

EC number: NA

Molecular weight: Translated: 40397; Mature: 40266

Theoretical pI: Translated: 7.72; Mature: 7.72

Prosite motif: PS00380 RHODANESE_1 ; PS50206 RHODANESE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPLTPNITADICPQLYSEIIDVRSPAEYAEDHLPGAINLPVLTDQERAEVGTLYKQVSAF
CCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHCCCCCCCCCEECCHHHHHHHHHHHHHHHH
VARKKGAALVSQNLSYHLKHHFADHPKGYHPLIYCWRGGQRSQSMALVLTQVGWRTTLLK
HHHHCCHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCHHHHHHHHHCHHHHHHH
GGYKTYRTYVREQLEQVPYHFSYRVLCGLTGTAKTEILQRLAQAQVQVLDLEGLANHRGS
CCHHHHHHHHHHHHHHCCCCCCCHHEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCC
LLGAAETSAQPSQKQFESDLLSALQTFDTAHPVWIEAESNKIGERFVPKALWDQMQAAPC
EEECCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHHHCCCC
VEVQAPMSSRVDWLVTHYEHFTHNSALLKDKLNLLRRSYSKAQLQHWHNLIDEQQWPAFV
CEECCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
QSLLEEHYDPAYRRSMKRQYTNIQQQHQLPDLSATSLQQLVNQLATNCNQPATTV
HHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
PLTPNITADICPQLYSEIIDVRSPAEYAEDHLPGAINLPVLTDQERAEVGTLYKQVSAF
CCCCCCCHHHHHHHHHHHHHCCCCHHHHHHCCCCCCCCCEECCHHHHHHHHHHHHHHHH
VARKKGAALVSQNLSYHLKHHFADHPKGYHPLIYCWRGGQRSQSMALVLTQVGWRTTLLK
HHHHCCHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCHHHHHHHHHCHHHHHHH
GGYKTYRTYVREQLEQVPYHFSYRVLCGLTGTAKTEILQRLAQAQVQVLDLEGLANHRGS
CCHHHHHHHHHHHHHHCCCCCCCHHEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCC
LLGAAETSAQPSQKQFESDLLSALQTFDTAHPVWIEAESNKIGERFVPKALWDQMQAAPC
EEECCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHHHCCCC
VEVQAPMSSRVDWLVTHYEHFTHNSALLKDKLNLLRRSYSKAQLQHWHNLIDEQQWPAFV
CEECCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
QSLLEEHYDPAYRRSMKRQYTNIQQQHQLPDLSATSLQQLVNQLATNCNQPATTV
HHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12700255 [H]