Click here to switch to the map view.

The map label for this gene is thrS [H]

Identifier: 158334707

GI number: 158334707

Start: 1523271

End: 1525091

Strand: Direct

Name: thrS [H]

Synonym: AM1_1540

Alternate gene names: 158334707

Gene position: 1523271-1525091 (Clockwise)

Preceding gene: 158334703

Following gene: 158334708

Centisome position: 23.42

GC content: 50.03

Gene sequence:

>1821_bases
ATGTCCGCTGAACAGCAACCTGAACAACAGCCTGAAAAGATTCACTTACCCAAGACCAGTGAATCCGAACAACTCAAGAA
AATCCGCCACACCACCTCTCATGTGATGGCGATGGCTGTTCAGAAGCTGTTTCCCGATGCCCAAGTTACCATTGGTCCGT
GGATTGAGTCCGGTTTTTATTATGACTTTGACAGCCCCACTCCTTTTACAGATAAAGATTTGAAAGCCATCAAAAAAGAG
ATGGTCAAAATCATTAAAAAGAAGCTGCCCGTAGAACGGGAAGAGGTGAGTCGGGAAGAGGCGAAGAAAAGAATAAAAGC
GTTAGGAGAACCTTATAAGCTGGAGATTCTGGAGGATCTAGAGGATCCGATTACCCTCTATCACCTTGGCGATCAGTGGT
GGGATTTGTGTGCCGGTCCCCATGTGGAGTCCACTGCCGATTTGAATCCTAAAGCGATTGCCCTAGAAAGCGTGGCGGGG
GCCTATTGGCGAGGGGATGAAACCAAAGCCCAATTACAGCGTATCTATGGCACGGCTTGGGAAACCCCTGAACAGTTAGC
AGAACATAAACGGCGGAAGGAAGAAGCCCTCCGTCGTGACCACCGAAAGCTCGGGAAAGAGCTGGGACTATTTCTCTTTT
CCGATCTGGTGGGACCGGGATTGCCCTTATGGACACCTAAGGGAACCTTGCTGCGATCGCAACTCGAAGACTTCCTCAAA
CAGGAACAAATCCAGCGAGGATATTTGGGGGTGACCACGCCCCATATTGCTCGGGTTGATCTGTTTAAGACTTCAGGGCA
CTGGCAAAAATACCAAGAAGATATGTTCCCGCTGATGAGTGAGAACGATGCAGATCGCAGCTCTGAACAAGGCTTTGTGA
TGAAGCCGATGAACTGTCCGTTTCATATCCAGATCTATAAGAGTGAGTTGCGGTCTTATCGCGACCTACCCATGCGGCTG
GCAGAATTTGGCACCGTTTATCGGTATGAGCAATCCGGCGAGCTAGGGGGCCTCACACGAGTCCGCGGCTTTACCGTCGA
TGACTCCCATTTATTTGTCACGCCTGATCAGCTCGATGATGAATTTCTCAAGGTGGTGGATTTAATTCTCTCCGTCTTTG
AGAAGCTGCAGCTCAAGAATTTCAAAGCCCGTCTCAGCTTCCGTGATCCAGATTCAGACAAATATATTGGTTCTGATGAA
GCTTGGGAAAAGTCTCAGAATGCCATTCGTCGAGCCGTGGAAACCTTGGGCATGGAGCATTTTGAAGGTATTGGCGAAGC
TGCTTTCTATGGTCCGAAGCTGGACTTTATTTTCCAAGATGTCCTGGATCGGGAATGGCAGTTGGGAACGGTACAGGTGG
ACTACAATCTACCCGAGCGCTTTGACCTGGAATTTATGGGAGAAGACGGTAACCGTCAGCGCCCGATTATGATTCACCGG
GCTCCCTTCGGATCTTTAGAACGACTGATTGGTATCTTAATCGAGGAATATGCAGGTGTCTTTCCCCTATGGCTAGCCCC
CGAACAGGTGCGGCTGTTGACGGTGAATGATGACCATATGCCCTTTGCCCAAGCGGTAGCCACGGATTTGCAGGCTGCGG
GGATTCGAGTCTCCGTGGATCAAAGTGGCGATCGCCTAGGCAAGAAAATTCGCAATGCGGAGAAAGCCAAAATCCCAGTG
ATGTCAGTGATTGGGGATAAGGAAGTCGAAGCCCATAGCCTCAATATTCGGACGCGGGCGTCAGGTGAGTTAGGGGCGAT
TCCGGTGGATGAGGTGAAGCAGAAGTTAGGCGATGCGATCTCAACTTTCTCTGCCTTCTAG

Upstream 100 bases:

>100_bases
TTGAGGTTTGAGGCCAAGTCTAACTTTATCGGTGATTCCTTCAAAGGTTTCGATAAGATGGGACAAGTCCGTAAAATTAG
CTCAAACATATAGATCCATA

Downstream 100 bases:

>100_bases
CCAATACACCCAAAACGTTAAGATCGGAGAAATTACGCTCGAATTTCGTCAACTCTCATCTCATGAAGAGATGAAAAATT
GTAATCAATAATTTTTGCAA

Product: threonyl-tRNA synthetase

Products: NA

Alternate protein names: Threonine--tRNA ligase; ThrRS [H]

Number of amino acids: Translated: 606; Mature: 605

Protein sequence:

>606_residues
MSAEQQPEQQPEKIHLPKTSESEQLKKIRHTTSHVMAMAVQKLFPDAQVTIGPWIESGFYYDFDSPTPFTDKDLKAIKKE
MVKIIKKKLPVEREEVSREEAKKRIKALGEPYKLEILEDLEDPITLYHLGDQWWDLCAGPHVESTADLNPKAIALESVAG
AYWRGDETKAQLQRIYGTAWETPEQLAEHKRRKEEALRRDHRKLGKELGLFLFSDLVGPGLPLWTPKGTLLRSQLEDFLK
QEQIQRGYLGVTTPHIARVDLFKTSGHWQKYQEDMFPLMSENDADRSSEQGFVMKPMNCPFHIQIYKSELRSYRDLPMRL
AEFGTVYRYEQSGELGGLTRVRGFTVDDSHLFVTPDQLDDEFLKVVDLILSVFEKLQLKNFKARLSFRDPDSDKYIGSDE
AWEKSQNAIRRAVETLGMEHFEGIGEAAFYGPKLDFIFQDVLDREWQLGTVQVDYNLPERFDLEFMGEDGNRQRPIMIHR
APFGSLERLIGILIEEYAGVFPLWLAPEQVRLLTVNDDHMPFAQAVATDLQAAGIRVSVDQSGDRLGKKIRNAEKAKIPV
MSVIGDKEVEAHSLNIRTRASGELGAIPVDEVKQKLGDAISTFSAF

Sequences:

>Translated_606_residues
MSAEQQPEQQPEKIHLPKTSESEQLKKIRHTTSHVMAMAVQKLFPDAQVTIGPWIESGFYYDFDSPTPFTDKDLKAIKKE
MVKIIKKKLPVEREEVSREEAKKRIKALGEPYKLEILEDLEDPITLYHLGDQWWDLCAGPHVESTADLNPKAIALESVAG
AYWRGDETKAQLQRIYGTAWETPEQLAEHKRRKEEALRRDHRKLGKELGLFLFSDLVGPGLPLWTPKGTLLRSQLEDFLK
QEQIQRGYLGVTTPHIARVDLFKTSGHWQKYQEDMFPLMSENDADRSSEQGFVMKPMNCPFHIQIYKSELRSYRDLPMRL
AEFGTVYRYEQSGELGGLTRVRGFTVDDSHLFVTPDQLDDEFLKVVDLILSVFEKLQLKNFKARLSFRDPDSDKYIGSDE
AWEKSQNAIRRAVETLGMEHFEGIGEAAFYGPKLDFIFQDVLDREWQLGTVQVDYNLPERFDLEFMGEDGNRQRPIMIHR
APFGSLERLIGILIEEYAGVFPLWLAPEQVRLLTVNDDHMPFAQAVATDLQAAGIRVSVDQSGDRLGKKIRNAEKAKIPV
MSVIGDKEVEAHSLNIRTRASGELGAIPVDEVKQKLGDAISTFSAF
>Mature_605_residues
SAEQQPEQQPEKIHLPKTSESEQLKKIRHTTSHVMAMAVQKLFPDAQVTIGPWIESGFYYDFDSPTPFTDKDLKAIKKEM
VKIIKKKLPVEREEVSREEAKKRIKALGEPYKLEILEDLEDPITLYHLGDQWWDLCAGPHVESTADLNPKAIALESVAGA
YWRGDETKAQLQRIYGTAWETPEQLAEHKRRKEEALRRDHRKLGKELGLFLFSDLVGPGLPLWTPKGTLLRSQLEDFLKQ
EQIQRGYLGVTTPHIARVDLFKTSGHWQKYQEDMFPLMSENDADRSSEQGFVMKPMNCPFHIQIYKSELRSYRDLPMRLA
EFGTVYRYEQSGELGGLTRVRGFTVDDSHLFVTPDQLDDEFLKVVDLILSVFEKLQLKNFKARLSFRDPDSDKYIGSDEA
WEKSQNAIRRAVETLGMEHFEGIGEAAFYGPKLDFIFQDVLDREWQLGTVQVDYNLPERFDLEFMGEDGNRQRPIMIHRA
PFGSLERLIGILIEEYAGVFPLWLAPEQVRLLTVNDDHMPFAQAVATDLQAAGIRVSVDQSGDRLGKKIRNAEKAKIPVM
SVIGDKEVEAHSLNIRTRASGELGAIPVDEVKQKLGDAISTFSAF

Specific function: Thrs Is Also A Translational Repressor Protein, It Controls The Translation Of Its Own Gene By Binding To Its mRNA. [C]

COG id: COG0441

COG function: function code J; Threonyl-tRNA synthetase

Gene ontology:
GO:0000166: Threonyl-tRNA synthetase
GO:0004812: Threonyl-tRNA synthetase
GO:0004829: Threonyl-tRNA synthetase
GO:0005524: Threonyl-tRNA synthetase
GO:0005737: Threonyl-tRNA synthetase
GO:0006418: Threonyl-tRNA synthetase
GO:0006435: Threonyl-tRNA synthetase
GO:0016876: Threonyl-tRNA synthetase
GO:0043039: Threonyl-tRNA synthetase

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI38202255, Length=595, Percent_Identity=35.7983193277311, Blast_Score=375, Evalue=1e-104,
Organism=Homo sapiens, GI38570111, Length=573, Percent_Identity=35.9511343804538, Blast_Score=364, Evalue=1e-100,
Organism=Homo sapiens, GI20070344, Length=581, Percent_Identity=35.6282271944923, Blast_Score=355, Evalue=9e-98,
Organism=Escherichia coli, GI1788013, Length=595, Percent_Identity=40, Blast_Score=461, Evalue=1e-131,
Organism=Escherichia coli, GI1786392, Length=168, Percent_Identity=25.5952380952381, Blast_Score=64, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI71984184, Length=594, Percent_Identity=36.026936026936, Blast_Score=394, Evalue=1e-110,
Organism=Caenorhabditis elegans, GI71984192, Length=594, Percent_Identity=36.026936026936, Blast_Score=394, Evalue=1e-110,
Organism=Saccharomyces cerevisiae, GI6322112, Length=598, Percent_Identity=35.9531772575251, Blast_Score=366, Evalue=1e-102,
Organism=Saccharomyces cerevisiae, GI6322654, Length=397, Percent_Identity=34.7607052896725, Blast_Score=239, Evalue=8e-64,
Organism=Drosophila melanogaster, GI24583842, Length=587, Percent_Identity=37.3083475298126, Blast_Score=399, Evalue=1e-111,
Organism=Drosophila melanogaster, GI28574141, Length=587, Percent_Identity=37.3083475298126, Blast_Score=399, Evalue=1e-111,
Organism=Drosophila melanogaster, GI24583839, Length=587, Percent_Identity=37.3083475298126, Blast_Score=399, Evalue=1e-111,

Paralogues:

None

Copy number: 700 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR002320
- InterPro:   IPR018163
- InterPro:   IPR012947 [H]

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b; PF07973 tRNA_SAD [H]

EC number: =6.1.1.3 [H]

Molecular weight: Translated: 69282; Mature: 69150

Theoretical pI: Translated: 5.39; Mature: 5.39

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAEQQPEQQPEKIHLPKTSESEQLKKIRHTTSHVMAMAVQKLFPDAQVTIGPWIESGFY
CCCCCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCEE
YDFDSPTPFTDKDLKAIKKEMVKIIKKKLPVEREEVSREEAKKRIKALGEPYKLEILEDL
EECCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCEEEHHHHHC
EDPITLYHLGDQWWDLCAGPHVESTADLNPKAIALESVAGAYWRGDETKAQLQRIYGTAW
CCCEEEEECCHHHHHHHCCCCCCCCCCCCCHHEEHHHHHCHHCCCCCHHHHHHHHHCCCC
ETPEQLAEHKRRKEEALRRDHRKLGKELGLFLFSDLVGPGLPLWTPKGTLLRSQLEDFLK
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHH
QEQIQRGYLGVTTPHIARVDLFKTSGHWQKYQEDMFPLMSENDADRSSEQGFVMKPMNCP
HHHHHCCCCCCCCCCEEEEEEEECCCCHHHHHHHHCCCCCCCCCCCCCCCCEEECCCCCC
FHIQIYKSELRSYRDLPMRLAEFGTVYRYEQSGELGGLTRVRGFTVDDSHLFVTPDQLDD
EEEEEEHHHHHHHHHHHHHHHHHCHHEEECCCCCCCCCCEEEEEEECCCEEEECCCCCCH
EFLKVVDLILSVFEKLQLKNFKARLSFRDPDSDKYIGSDEAWEKSQNAIRRAVETLGMEH
HHHHHHHHHHHHHHHHHHHCHHEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHCHHH
FEGIGEAAFYGPKLDFIFQDVLDREWQLGTVQVDYNLPERFDLEFMGEDGNRQRPIMIHR
HHCCCCHHHCCCCHHHHHHHHHCCCCEEEEEEEECCCCCCCCEEEECCCCCCCCCEEEEE
APFGSLERLIGILIEEYAGVFPLWLAPEQVRLLTVNDDHMPFAQAVATDLQAAGIRVSVD
CCCHHHHHHHHHHHHHHCCCCEEEECCCCEEEEEECCCCCCHHHHHHHHHHHCCEEEEEC
QSGDRLGKKIRNAEKAKIPVMSVIGDKEVEAHSLNIRTRASGELGAIPVDEVKQKLGDAI
CCHHHHHHHHHCCHHHCCCHHHHCCCCCCCEEEEEEEEECCCCCCCCCHHHHHHHHHHHH
STFSAF
HHHHCC
>Mature Secondary Structure 
SAEQQPEQQPEKIHLPKTSESEQLKKIRHTTSHVMAMAVQKLFPDAQVTIGPWIESGFY
CCCCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCEE
YDFDSPTPFTDKDLKAIKKEMVKIIKKKLPVEREEVSREEAKKRIKALGEPYKLEILEDL
EECCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCEEEHHHHHC
EDPITLYHLGDQWWDLCAGPHVESTADLNPKAIALESVAGAYWRGDETKAQLQRIYGTAW
CCCEEEEECCHHHHHHHCCCCCCCCCCCCCHHEEHHHHHCHHCCCCCHHHHHHHHHCCCC
ETPEQLAEHKRRKEEALRRDHRKLGKELGLFLFSDLVGPGLPLWTPKGTLLRSQLEDFLK
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHH
QEQIQRGYLGVTTPHIARVDLFKTSGHWQKYQEDMFPLMSENDADRSSEQGFVMKPMNCP
HHHHHCCCCCCCCCCEEEEEEEECCCCHHHHHHHHCCCCCCCCCCCCCCCCEEECCCCCC
FHIQIYKSELRSYRDLPMRLAEFGTVYRYEQSGELGGLTRVRGFTVDDSHLFVTPDQLDD
EEEEEEHHHHHHHHHHHHHHHHHCHHEEECCCCCCCCCCEEEEEEECCCEEEECCCCCCH
EFLKVVDLILSVFEKLQLKNFKARLSFRDPDSDKYIGSDEAWEKSQNAIRRAVETLGMEH
HHHHHHHHHHHHHHHHHHHCHHEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHCHHH
FEGIGEAAFYGPKLDFIFQDVLDREWQLGTVQVDYNLPERFDLEFMGEDGNRQRPIMIHR
HHCCCCHHHCCCCHHHHHHHHHCCCCEEEEEEEECCCCCCCCEEEECCCCCCCCCEEEEE
APFGSLERLIGILIEEYAGVFPLWLAPEQVRLLTVNDDHMPFAQAVATDLQAAGIRVSVD
CCCHHHHHHHHHHHHHHCCCCEEEECCCCEEEEEECCCCCCHHHHHHHHHHHCCEEEEEC
QSGDRLGKKIRNAEKAKIPVMSVIGDKEVEAHSLNIRTRASGELGAIPVDEVKQKLGDAI
CCHHHHHHHHHCCHHHCCCHHHHCCCCCCCEEEEEEEEECCCCCCCCCHHHHHHHHHHHH
STFSAF
HHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]