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The map label for this gene is trmH [C]

Identifier: 158334661

GI number: 158334661

Start: 1478775

End: 1479560

Strand: Direct

Name: trmH [C]

Synonym: AM1_1493

Alternate gene names: 158334661

Gene position: 1478775-1479560 (Clockwise)

Preceding gene: 158334660

Following gene: 158334662

Centisome position: 22.74

GC content: 52.16

Gene sequence:

>786_bases
ATGATTACCAGCTTGCAAAATCCGCTGATTAAACAAATGCGGAAGTTGCAGCAGGTCAAACATCGCCGTCAGCAAGGCAA
GCTGCTGCTCGAAGGCACCCATTTACTCCAGTCTGCCTGGGAGCAAAATTGGTCCTTTGAGATTGTTTGCTATACAGATA
GATGGCAAGCCAAATATCCCCAGATATGGCAGCAAATTCAAGACCGAACGACTCGGTTGGAGCGGGTCAGCCCAGAGGTC
TTGCAAGCCACTGCGACAACCGTGCATCCTGATGGCGTGGTAGCAGTCATTAGTCGGCCAGCAACACCATCGTTACCGAT
TCAGACTTTAGGACTAGCCCTAGAAACCATTCAAGATCCGGGCAATCTTGGGACCTTAATTCGGACCTCTGTGGCGACTC
AGGTGGAAGGGCTGCTGCTCAATCAACAGTGTGCAGATCTAGAAAGTCCCAAAGTCTTGAGGGCTTCGGCTGGAGCCTGG
TTTCATCTTCCCCAGCGGGCAACGGATAAATTCGTCACCGTATTACAAACGTATAAACAGCAGGGCATGCAGCTTATTGC
CACGGTTCCCGATGCATCCCACACCTTTTGGCAGGTTGATTATCGCCAACCCACCCTGATCTTGATTGGCAATGAAGGAG
CAGGTTTATCCCAGCCTGTGTTGGATTTGGCCGATCACCAGGTCAATATTCCCATTGCCCCAGGGGTGGAGTCGTTGAAT
GCTGCGATCGCAGCCGCGCTTCTCCTCTACGAAGTCCGTCGGCAGCGTGATGGCAAACCCGCTTGA

Upstream 100 bases:

>100_bases
CCAGCGGAACTGGCGGAATTGGTAGACGCGCTAGGTTCAGGTCCTAGTGTCTGCAAAGACATCCGGGTTCAAGTCCCGGG
TTCCGCATCTTTTACACCCT

Downstream 100 bases:

>100_bases
TTACGTTCAATAACATCCAATGGATTCCGCCCCAGAAGCCCATAGAATAAAACTACCTAGTAAAGCTGCTGCAATTTTTA
AAAATTTTCTATGACCACTG

Product: RNA methyltransferase

Products: S-adenosyl-L-homocysteine; tRNATyr Containing 2'-O-methylguanosine [C]

Alternate protein names: NA

Number of amino acids: Translated: 261; Mature: 261

Protein sequence:

>261_residues
MITSLQNPLIKQMRKLQQVKHRRQQGKLLLEGTHLLQSAWEQNWSFEIVCYTDRWQAKYPQIWQQIQDRTTRLERVSPEV
LQATATTVHPDGVVAVISRPATPSLPIQTLGLALETIQDPGNLGTLIRTSVATQVEGLLLNQQCADLESPKVLRASAGAW
FHLPQRATDKFVTVLQTYKQQGMQLIATVPDASHTFWQVDYRQPTLILIGNEGAGLSQPVLDLADHQVNIPIAPGVESLN
AAIAAALLLYEVRRQRDGKPA

Sequences:

>Translated_261_residues
MITSLQNPLIKQMRKLQQVKHRRQQGKLLLEGTHLLQSAWEQNWSFEIVCYTDRWQAKYPQIWQQIQDRTTRLERVSPEV
LQATATTVHPDGVVAVISRPATPSLPIQTLGLALETIQDPGNLGTLIRTSVATQVEGLLLNQQCADLESPKVLRASAGAW
FHLPQRATDKFVTVLQTYKQQGMQLIATVPDASHTFWQVDYRQPTLILIGNEGAGLSQPVLDLADHQVNIPIAPGVESLN
AAIAAALLLYEVRRQRDGKPA
>Mature_261_residues
MITSLQNPLIKQMRKLQQVKHRRQQGKLLLEGTHLLQSAWEQNWSFEIVCYTDRWQAKYPQIWQQIQDRTTRLERVSPEV
LQATATTVHPDGVVAVISRPATPSLPIQTLGLALETIQDPGNLGTLIRTSVATQVEGLLLNQQCADLESPKVLRASAGAW
FHLPQRATDKFVTVLQTYKQQGMQLIATVPDASHTFWQVDYRQPTLILIGNEGAGLSQPVLDLADHQVNIPIAPGVESLN
AAIAAALLLYEVRRQRDGKPA

Specific function: Specifically Methylates Guanosine-18 In Various tRNAs. [C]

COG id: COG0566

COG function: function code J; rRNA methylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family [H]

Homologues:

Organism=Homo sapiens, GI8922534, Length=296, Percent_Identity=27.027027027027, Blast_Score=92, Evalue=7e-19,
Organism=Homo sapiens, GI40068479, Length=175, Percent_Identity=33.7142857142857, Blast_Score=83, Evalue=3e-16,
Organism=Escherichia coli, GI1790083, Length=148, Percent_Identity=33.7837837837838, Blast_Score=84, Evalue=7e-18,
Organism=Escherichia coli, GI1790623, Length=145, Percent_Identity=24.8275862068966, Blast_Score=68, Evalue=6e-13,
Organism=Escherichia coli, GI1788935, Length=182, Percent_Identity=27.4725274725275, Blast_Score=66, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24666840, Length=291, Percent_Identity=25.085910652921, Blast_Score=87, Evalue=1e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001537
- InterPro:   IPR013123 [H]

Pfam domain/function: PF00588 SpoU_methylase; PF08032 SpoU_sub_bind [H]

EC number: 2.1.1.34 [C]

Molecular weight: Translated: 29101; Mature: 29101

Theoretical pI: Translated: 8.70; Mature: 8.70

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MITSLQNPLIKQMRKLQQVKHRRQQGKLLLEGTHLLQSAWEQNWSFEIVCYTDRWQAKYP
CCCCCCCHHHHHHHHHHHHHHHHHHCCEEEHHHHHHHHHHHCCCCEEEEEEECCCCCHHH
QIWQQIQDRTTRLERVSPEVLQATATTVHPDGVVAVISRPATPSLPIQTLGLALETIQDP
HHHHHHHHHHHHHHHCCHHHHHHHHCEECCCCEEEEEECCCCCCCCHHHHHHHHHHHCCC
GNLGTLIRTSVATQVEGLLLNQQCADLESPKVLRASAGAWFHLPQRATDKFVTVLQTYKQ
CCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCEEECCHHHHHHHHHHHHHHHH
QGMQLIATVPDASHTFWQVDYRQPTLILIGNEGAGLSQPVLDLADHQVNIPIAPGVESLN
CCCEEEEECCCCCCCEEEEECCCCEEEEECCCCCCCCCHHHHHCCCEEECCCCCCHHHHH
AAIAAALLLYEVRRQRDGKPA
HHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MITSLQNPLIKQMRKLQQVKHRRQQGKLLLEGTHLLQSAWEQNWSFEIVCYTDRWQAKYP
CCCCCCCHHHHHHHHHHHHHHHHHHCCEEEHHHHHHHHHHHCCCCEEEEEEECCCCCHHH
QIWQQIQDRTTRLERVSPEVLQATATTVHPDGVVAVISRPATPSLPIQTLGLALETIQDP
HHHHHHHHHHHHHHHCCHHHHHHHHCEECCCCEEEEEECCCCCCCCHHHHHHHHHHHCCC
GNLGTLIRTSVATQVEGLLLNQQCADLESPKVLRASAGAWFHLPQRATDKFVTVLQTYKQ
CCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCEEECCHHHHHHHHHHHHHHHH
QGMQLIATVPDASHTFWQVDYRQPTLILIGNEGAGLSQPVLDLADHQVNIPIAPGVESLN
CCCEEEEECCCCCCCEEEEECCCCEEEEECCCCCCCCCHHHHHCCCEEECCCCCCHHHHH
AAIAAALLLYEVRRQRDGKPA
HHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: S-Adenosyl-L-methionine; tRNATyr [C]

Specific reaction: S-Adenosyl-L-methionine + tRNATyr = S-adenosyl-L-homocysteine + tRNATyr Containing 2'-O-methylguanosine [C]

General reaction: Methyl group transfer [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]