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The map label for this gene is pcrA [H]

Identifier: 158334598

GI number: 158334598

Start: 1420257

End: 1422599

Strand: Reverse

Name: pcrA [H]

Synonym: AM1_1428

Alternate gene names: 158334598

Gene position: 1422599-1420257 (Counterclockwise)

Preceding gene: 158334599

Following gene: 158334597

Centisome position: 21.87

GC content: 48.61

Gene sequence:

>2343_bases
ATGTCTGCTATTCCTGACTTTTTAAGTCATCTCAATCCTTCCCAGCGACAGGCAGTGGAACACTTCTGTGGCCCGCTGTT
GGTGGTAGCGGGGGCTGGATCGGGTAAAACTCGAGCCTTGACCTATCGCATCTCTAATTTGGTGATGCGCCATCAGGTTA
ATCCCGAAAATATTCTGGCCGTGACGTTTACGAATAAAGCGGCTCGGGAGATGAAGGAGCGGATTGAAAAATTATTTGCG
GAACAGCTGGCCCAGCAGGATCATGGCAAAGCACTGGAGCTGTTAGCACCTCAGCAGCAGACAAAGTTGCGATCGCAAGT
CTATAAAACCATTACCAAACCCCTCTGGATTGGCACATTCCATAGTCTCTGTGCCCGCCTGTTGCGGATGGAAATCGAAA
AATACCAGGATGAGAAGGGCCGCAAATGGCAGCGTAATTTCTCTATTTTTGACGAATCAGATGTTCAGAGTCTGATTAAA
GATATTGTGGTCAATCAGCTGAATCTCGACGATCGTAAATTCCAGCCTCGTTCCGTCCGCTTTGCTATAAGTAATGCTAA
AAACCAGGGCTGGAACCCCCAAGAGCTGGAAGCCAATCAACAGGATTTTCGCGGTCGCGTGGTAGCCCAGGTCTACAGCA
AATATCAAGATTCCCTAGCTGCCAATAACGGCCTGGATTTTGATGATCTGATTCGGGTGCTGGTGCAGTTGCTCAAGCAG
AATGGCCAGGTTCTCGGCTATTGGCACCAGCAGTTTCGCCATATGCTGGTGGACGAATACCAAGACACTAACCGCACCCA
ATATGATTTGCTGCGGTTACTAGCGACCAACGGCACCGATACTCGCAACTTTAAGGATTGGGAGCATCGCTCTGTCTTTG
TAGTGGGTGATGCTGATCAGTCCATTTACTCCTTTCGGGCAGCGGACTTTAAAATCCTGATGGGCTTTCAGCAGGATTTT
GGTGATGGCCTGCCGGATGGTGATACGCGGACGATGGTGAAGCTAGAGGAGAACTATCGGTCTACCGAAAACATCCTCCA
AGTGGCCAATGAACTCATCGATAACAATACTGAACGTATCGACAAAATTCTCAAGCCGACTCGGGGAGCAGGAGAACCCA
TCTACTGCTATCGGGCAGATGATGAACTGCATGAATCTGATTTCGTCATTCAGCAAATTCGGACTCTAGAGCAATCCCAC
CCCGAACTGAGCTGGGGAGACTTTGCTATTCTCTATCGGACTAATGCCCAATCTCGGGCATTTGAAGAGGGCTTAGTTCG
TTGGAGTATTCCCTATACGGTGGTCGGGGGACTGAAGTTCTACGATCGCCGCGAGATTAAGGATGTCTTGGCCTATCTGC
GCCTAATCGTTAATCCGGCGGATACGGTCAGCCTCAAACGGGTGATTAATACACCCCGCAGAGGAATTGGCAAAACCACC
TTAGATCGCCTTATGAATGCAGCCCAGGAGCTAGGAGTGCCCCTATGGGAAATGCTGACAGAAGAGACGGCAGTCAAGAC
CTTAGCTGCTCGTAGCGCCAAGCCAATTTTGCAGTTTGTGGAAATGATGCGACATTGGCAAGGTCAGGTGGAAACCCAAT
CTGCAGCCAATATTATCCAGGGGATCTTAGAAGATTCGGGGTATGTTCGGGACTTAAAAAATCAGGGAACTGATGAAGCA
GATGATCGAATTGGCAATGTCCAAGAATTGCAAAATGCCGCTTTACAATATGCAGAAGAGAATACCGATGAGTCTCTCCC
TTCTTTTCTCGCTAATACGGCCTTGGCCTCTGATTTAGATGATTTAGATGAAAAAACTACGGTGTCACTGATGACATTGC
ATGCAGCTAAGGGGTTGGAATTTCCCATCGTCTTTTTGGTTGGCCTAGAACAGGGATTGTTCCCTAATTTCCGCTCTTTA
GAAGATCCTGCGGCTATCGAAGAAGAACGCCGTCTTTGCTATGTTGGCGTTACTCGGGCTCAAGAAAGACTCTTTATTTC
TCACGCACGAGAGCGACGACTCTATGGTTCAAGGGAGCCAGCGATGCCTTCACTGTTTCTATCAGAGCTGCCTCGAGATC
TCTTACAGACTAATTCTTTATCGGCCATCCCGTCTGAGCCGCCCCATTCTGCAATGAGCCCAGGGGCTAAAAAGAAAAAA
ATGCCCAATACCCATGCCATGGATTGGGCGGTCGGTGATCGGCTGGTTCATCGCGGGTTTGGACTAGGGGAGGTGACCCA
TATTTTTGGAGGTGGGAATAAAATCTGCTTAGCAGTTAAATTTCCAGGGATTGGCAAAAAGATTATTGACCCGAATATCA
CGGTATTGGATCGGGTGGAATGA

Upstream 100 bases:

>100_bases
ATCACGTATGGTGGGCACAGAGATGGTTCGATTACTCAATCCCATTGGCATAACATCTCTGCTAACGACGATACTTCTGA
ATTCTTCTCCTTGATTTTTT

Downstream 100 bases:

>100_bases
AATCGAAATTCAAGTTATGGAGATACGAGCATAACGAAGTATGCGCTTGATCGTTTTTGATTTTGACGGCACCCTTGCCG
ATTCCCTTAGCATATTTATC

Product: ATP-dependent DNA helicase PcrA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 780; Mature: 779

Protein sequence:

>780_residues
MSAIPDFLSHLNPSQRQAVEHFCGPLLVVAGAGSGKTRALTYRISNLVMRHQVNPENILAVTFTNKAAREMKERIEKLFA
EQLAQQDHGKALELLAPQQQTKLRSQVYKTITKPLWIGTFHSLCARLLRMEIEKYQDEKGRKWQRNFSIFDESDVQSLIK
DIVVNQLNLDDRKFQPRSVRFAISNAKNQGWNPQELEANQQDFRGRVVAQVYSKYQDSLAANNGLDFDDLIRVLVQLLKQ
NGQVLGYWHQQFRHMLVDEYQDTNRTQYDLLRLLATNGTDTRNFKDWEHRSVFVVGDADQSIYSFRAADFKILMGFQQDF
GDGLPDGDTRTMVKLEENYRSTENILQVANELIDNNTERIDKILKPTRGAGEPIYCYRADDELHESDFVIQQIRTLEQSH
PELSWGDFAILYRTNAQSRAFEEGLVRWSIPYTVVGGLKFYDRREIKDVLAYLRLIVNPADTVSLKRVINTPRRGIGKTT
LDRLMNAAQELGVPLWEMLTEETAVKTLAARSAKPILQFVEMMRHWQGQVETQSAANIIQGILEDSGYVRDLKNQGTDEA
DDRIGNVQELQNAALQYAEENTDESLPSFLANTALASDLDDLDEKTTVSLMTLHAAKGLEFPIVFLVGLEQGLFPNFRSL
EDPAAIEEERRLCYVGVTRAQERLFISHARERRLYGSREPAMPSLFLSELPRDLLQTNSLSAIPSEPPHSAMSPGAKKKK
MPNTHAMDWAVGDRLVHRGFGLGEVTHIFGGGNKICLAVKFPGIGKKIIDPNITVLDRVE

Sequences:

>Translated_780_residues
MSAIPDFLSHLNPSQRQAVEHFCGPLLVVAGAGSGKTRALTYRISNLVMRHQVNPENILAVTFTNKAAREMKERIEKLFA
EQLAQQDHGKALELLAPQQQTKLRSQVYKTITKPLWIGTFHSLCARLLRMEIEKYQDEKGRKWQRNFSIFDESDVQSLIK
DIVVNQLNLDDRKFQPRSVRFAISNAKNQGWNPQELEANQQDFRGRVVAQVYSKYQDSLAANNGLDFDDLIRVLVQLLKQ
NGQVLGYWHQQFRHMLVDEYQDTNRTQYDLLRLLATNGTDTRNFKDWEHRSVFVVGDADQSIYSFRAADFKILMGFQQDF
GDGLPDGDTRTMVKLEENYRSTENILQVANELIDNNTERIDKILKPTRGAGEPIYCYRADDELHESDFVIQQIRTLEQSH
PELSWGDFAILYRTNAQSRAFEEGLVRWSIPYTVVGGLKFYDRREIKDVLAYLRLIVNPADTVSLKRVINTPRRGIGKTT
LDRLMNAAQELGVPLWEMLTEETAVKTLAARSAKPILQFVEMMRHWQGQVETQSAANIIQGILEDSGYVRDLKNQGTDEA
DDRIGNVQELQNAALQYAEENTDESLPSFLANTALASDLDDLDEKTTVSLMTLHAAKGLEFPIVFLVGLEQGLFPNFRSL
EDPAAIEEERRLCYVGVTRAQERLFISHARERRLYGSREPAMPSLFLSELPRDLLQTNSLSAIPSEPPHSAMSPGAKKKK
MPNTHAMDWAVGDRLVHRGFGLGEVTHIFGGGNKICLAVKFPGIGKKIIDPNITVLDRVE
>Mature_779_residues
SAIPDFLSHLNPSQRQAVEHFCGPLLVVAGAGSGKTRALTYRISNLVMRHQVNPENILAVTFTNKAAREMKERIEKLFAE
QLAQQDHGKALELLAPQQQTKLRSQVYKTITKPLWIGTFHSLCARLLRMEIEKYQDEKGRKWQRNFSIFDESDVQSLIKD
IVVNQLNLDDRKFQPRSVRFAISNAKNQGWNPQELEANQQDFRGRVVAQVYSKYQDSLAANNGLDFDDLIRVLVQLLKQN
GQVLGYWHQQFRHMLVDEYQDTNRTQYDLLRLLATNGTDTRNFKDWEHRSVFVVGDADQSIYSFRAADFKILMGFQQDFG
DGLPDGDTRTMVKLEENYRSTENILQVANELIDNNTERIDKILKPTRGAGEPIYCYRADDELHESDFVIQQIRTLEQSHP
ELSWGDFAILYRTNAQSRAFEEGLVRWSIPYTVVGGLKFYDRREIKDVLAYLRLIVNPADTVSLKRVINTPRRGIGKTTL
DRLMNAAQELGVPLWEMLTEETAVKTLAARSAKPILQFVEMMRHWQGQVETQSAANIIQGILEDSGYVRDLKNQGTDEAD
DRIGNVQELQNAALQYAEENTDESLPSFLANTALASDLDDLDEKTTVSLMTLHAAKGLEFPIVFLVGLEQGLFPNFRSLE
DPAAIEEERRLCYVGVTRAQERLFISHARERRLYGSREPAMPSLFLSELPRDLLQTNSLSAIPSEPPHSAMSPGAKKKKM
PNTHAMDWAVGDRLVHRGFGLGEVTHIFGGGNKICLAVKFPGIGKKIIDPNITVLDRVE

Specific function: Essential helicase. May act as a helicase in plasmid pT181 replication [H]

COG id: COG0210

COG function: function code L; Superfamily I DNA and RNA helicases

Gene ontology:
GO:0000166: ATP-dependent DNA helicase PcrA
GO:0003677: ATP-dependent DNA helicase PcrA
GO:0004003: ATP-dependent DNA helicase PcrA
GO:0004386: ATP-dependent DNA helicase PcrA
GO:0005524: ATP-dependent DNA helicase PcrA
GO:0005737: ATP-dependent DNA helicase PcrA
GO:0006268: ATP-dependent DNA helicase PcrA
GO:0006281: ATP-dependent DNA helicase PcrA
GO:0016787: ATP-dependent DNA helicase PcrA

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 uvrD-like helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI2367296, Length=782, Percent_Identity=36.4450127877238, Blast_Score=446, Evalue=1e-126,
Organism=Escherichia coli, GI48994965, Length=697, Percent_Identity=36.5853658536585, Blast_Score=365, Evalue=1e-102,
Organism=Escherichia coli, GI1787196, Length=377, Percent_Identity=28.6472148541114, Blast_Score=116, Evalue=6e-27,
Organism=Saccharomyces cerevisiae, GI6322369, Length=781, Percent_Identity=27.1446862996159, Blast_Score=217, Evalue=7e-57,
Organism=Saccharomyces cerevisiae, GI6324477, Length=480, Percent_Identity=23.9583333333333, Blast_Score=93, Evalue=2e-19,

Paralogues:

None

Copy number: 3000 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005751
- InterPro:   IPR013986
- InterPro:   IPR014017
- InterPro:   IPR000212
- InterPro:   IPR014016 [H]

Pfam domain/function: PF00580 UvrD-helicase [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 88651; Mature: 88520

Theoretical pI: Translated: 6.29; Mature: 6.29

Prosite motif: PS00086 CYTOCHROME_P450

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAIPDFLSHLNPSQRQAVEHFCGPLLVVAGAGSGKTRALTYRISNLVMRHQVNPENILA
CCCCHHHHHHCCCHHHHHHHHHCCCEEEEEECCCCCCEEEHHHHHHHHHHHCCCCCCEEE
VTFTNKAAREMKERIEKLFAEQLAQQDHGKALELLAPQQQTKLRSQVYKTITKPLWIGTF
EEECCHHHHHHHHHHHHHHHHHHHHHHCCCEEHHCCCHHHHHHHHHHHHHHHHHHHHHHH
HSLCARLLRMEIEKYQDEKGRKWQRNFSIFDESDVQSLIKDIVVNQLNLDDRKFQPRSVR
HHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCEEE
FAISNAKNQGWNPQELEANQQDFRGRVVAQVYSKYQDSLAANNGLDFDDLIRVLVQLLKQ
EEEECCCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHC
NGQVLGYWHQQFRHMLVDEYQDTNRTQYDLLRLLATNGTDTRNFKDWEHRSVFVVGDADQ
CCCEEEHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEECCCH
SIYSFRAADFKILMGFQQDFGDGLPDGDTRTMVKLEENYRSTENILQVANELIDNNTERI
HHHHHHHCCHHHEECHHHHHCCCCCCCCCCEEEEEHHHCCHHHHHHHHHHHHHCCCHHHH
DKILKPTRGAGEPIYCYRADDELHESDFVIQQIRTLEQSHPELSWGDFAILYRTNAQSRA
HHHHCCCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCHHHH
FEEGLVRWSIPYTVVGGLKFYDRREIKDVLAYLRLIVNPADTVSLKRVINTPRRGIGKTT
HHHCCEEEECCHHEECCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCHHH
LDRLMNAAQELGVPLWEMLTEETAVKTLAARSAKPILQFVEMMRHWQGQVETQSAANIIQ
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCCCCHHHHHHHHH
GILEDSGYVRDLKNQGTDEADDRIGNVQELQNAALQYAEENTDESLPSFLANTALASDLD
HHHHCCCHHHHHHHCCCCCHHHHCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
DLDEKTTVSLMTLHAAKGLEFPIVFLVGLEQGLFPNFRSLEDPAAIEEERRLCYVGVTRA
HCCCHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCCHHHHHCCCEEEEEHHHH
QERLFISHARERRLYGSREPAMPSLFLSELPRDLLQTNSLSAIPSEPPHSAMSPGAKKKK
HHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCHHHHHCCCCCCCCCCCCCCCCCCCCHHCC
MPNTHAMDWAVGDRLVHRGFGLGEVTHIFGGGNKICLAVKFPGIGKKIIDPNITVLDRVE
CCCCCCHHHHHHHHHHHCCCCCCHHHHEECCCCEEEEEEECCCCCCHHCCCCCCEEECCC
>Mature Secondary Structure 
SAIPDFLSHLNPSQRQAVEHFCGPLLVVAGAGSGKTRALTYRISNLVMRHQVNPENILA
CCCHHHHHHCCCHHHHHHHHHCCCEEEEEECCCCCCEEEHHHHHHHHHHHCCCCCCEEE
VTFTNKAAREMKERIEKLFAEQLAQQDHGKALELLAPQQQTKLRSQVYKTITKPLWIGTF
EEECCHHHHHHHHHHHHHHHHHHHHHHCCCEEHHCCCHHHHHHHHHHHHHHHHHHHHHHH
HSLCARLLRMEIEKYQDEKGRKWQRNFSIFDESDVQSLIKDIVVNQLNLDDRKFQPRSVR
HHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCEEE
FAISNAKNQGWNPQELEANQQDFRGRVVAQVYSKYQDSLAANNGLDFDDLIRVLVQLLKQ
EEEECCCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHC
NGQVLGYWHQQFRHMLVDEYQDTNRTQYDLLRLLATNGTDTRNFKDWEHRSVFVVGDADQ
CCCEEEHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEECCCH
SIYSFRAADFKILMGFQQDFGDGLPDGDTRTMVKLEENYRSTENILQVANELIDNNTERI
HHHHHHHCCHHHEECHHHHHCCCCCCCCCCEEEEEHHHCCHHHHHHHHHHHHHCCCHHHH
DKILKPTRGAGEPIYCYRADDELHESDFVIQQIRTLEQSHPELSWGDFAILYRTNAQSRA
HHHHCCCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCHHHH
FEEGLVRWSIPYTVVGGLKFYDRREIKDVLAYLRLIVNPADTVSLKRVINTPRRGIGKTT
HHHCCEEEECCHHEECCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCHHH
LDRLMNAAQELGVPLWEMLTEETAVKTLAARSAKPILQFVEMMRHWQGQVETQSAANIIQ
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCCCCHHHHHHHHH
GILEDSGYVRDLKNQGTDEADDRIGNVQELQNAALQYAEENTDESLPSFLANTALASDLD
HHHHCCCHHHHHHHCCCCCHHHHCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
DLDEKTTVSLMTLHAAKGLEFPIVFLVGLEQGLFPNFRSLEDPAAIEEERRLCYVGVTRA
HCCCHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCCHHHHHCCCEEEEEHHHH
QERLFISHARERRLYGSREPAMPSLFLSELPRDLLQTNSLSAIPSEPPHSAMSPGAKKKK
HHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCHHHHHCCCCCCCCCCCCCCCCCCCCHHCC
MPNTHAMDWAVGDRLVHRGFGLGEVTHIFGGGNKICLAVKFPGIGKKIIDPNITVLDRVE
CCCCCCHHHHHHHHHHHCCCCCCHHHHEECCCCEEEEEEECCCCCCHHCCCCCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8232203 [H]