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The map label for this gene is dus [H]

Identifier: 158334547

GI number: 158334547

Start: 1350264

End: 1351292

Strand: Direct

Name: dus [H]

Synonym: AM1_1374

Alternate gene names: 158334547

Gene position: 1350264-1351292 (Clockwise)

Preceding gene: 158334545

Following gene: 158334548

Centisome position: 20.76

GC content: 50.73

Gene sequence:

>1029_bases
ATGTCTGCGCCTTCCATTAACCCAGCCAAACTCAGGAGTGAACTATCTACGCCCCTTGAGATCGGCCCCTTTGCGGTGAA
TAGTCGGGTTCTACAGTCACCCTTGTCGGGTGTCACAGATTTGGTCTTTCGCCGCTTGGTACGACGATACGCGCCTGAAT
CGATGCTCTACACTGAAATGGTTCATGCTAGCCAGGTCTGTCATGCCCGTGAACTCTCCAAAGTCATGGATGTGGATTTG
GATGAGCAGCCGATCAGCGTACAGCTGTTTGATTGCCGTCCCCATTTTCTAGCGGAAGCGGCCCAAATGGCCGTTCAGGA
AGGGGCAGACACCATTGATTTAAACATGGGCTGTCCCGTCAATAAGATCACCAAAAAAGGGGGCGGTTCTTCGCTACTAC
GAGATCCAGAAACGGCTGAAGCCATCGTTCGAGCAGTGGTGTCAGCAGTTAAAGTGCCCGTGACAGTCAAGACTCGTATT
GGCTGGAAAGAGGACGAAATTAACATCCTGGAGTTTGCCCAACGGATGCAGGATGCGGGGGCAAAAATGATTACGGTGCA
TGGACGCACCCGAGCCCAAGGCTATAACGGACCCGCTCAGTGGCATTGGATAAAGCAAGTTAAAGAGAAACTGTCGATTC
CAGTGATTGCCAACGGCGATATTGTCTCCGTGGAATCGGCGATGGCCTGTTTGGAAATGACCGGGGCAGATGGGGTGATG
TGTTCACGTGGAACACTCGGGTATCCCTATTTGGTAGGAGAAATTGATTATTTCTTAAAAACCGGACAGCAAAAGGCCCC
GCCCACCATTATTGAGCGTTTGCAATGTGCGCGGGAGCATTTGCAAGGGTTATGGGAGTACAAAGGCATCAAAGGGATTC
ATCAAGCCCGTAAGCATATGACTTGGTATGTGAAGGGGTTTGTTGGTGCAGCTGAATTACGAGATCGCCTCTGTCGGATC
AATAGCGTCGAAGAAGGTCTAGATTTATTGGACCAAACCATAGACGGTAATCCCACCCCGCAGACTTAA

Upstream 100 bases:

>100_bases
AGATTCCTCTTTACCTTTGCTGGAGGGCAAACCCAATAGAAATGCAGCACAACGGGTATTTTTGCCCACTACATCTTAGA
TAATCTTCCCAGTCTTCATA

Downstream 100 bases:

>100_bases
CCCAGTTCCCTACAATGTAGAAATGGACTGCTCCATCGATGTAGCTCCATTTCTCATGAGTAACTATGGGATTGACCTAG
ACTTTTTGATCAAACATACA

Product: dihydrouridine synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 342; Mature: 341

Protein sequence:

>342_residues
MSAPSINPAKLRSELSTPLEIGPFAVNSRVLQSPLSGVTDLVFRRLVRRYAPESMLYTEMVHASQVCHARELSKVMDVDL
DEQPISVQLFDCRPHFLAEAAQMAVQEGADTIDLNMGCPVNKITKKGGGSSLLRDPETAEAIVRAVVSAVKVPVTVKTRI
GWKEDEINILEFAQRMQDAGAKMITVHGRTRAQGYNGPAQWHWIKQVKEKLSIPVIANGDIVSVESAMACLEMTGADGVM
CSRGTLGYPYLVGEIDYFLKTGQQKAPPTIIERLQCAREHLQGLWEYKGIKGIHQARKHMTWYVKGFVGAAELRDRLCRI
NSVEEGLDLLDQTIDGNPTPQT

Sequences:

>Translated_342_residues
MSAPSINPAKLRSELSTPLEIGPFAVNSRVLQSPLSGVTDLVFRRLVRRYAPESMLYTEMVHASQVCHARELSKVMDVDL
DEQPISVQLFDCRPHFLAEAAQMAVQEGADTIDLNMGCPVNKITKKGGGSSLLRDPETAEAIVRAVVSAVKVPVTVKTRI
GWKEDEINILEFAQRMQDAGAKMITVHGRTRAQGYNGPAQWHWIKQVKEKLSIPVIANGDIVSVESAMACLEMTGADGVM
CSRGTLGYPYLVGEIDYFLKTGQQKAPPTIIERLQCAREHLQGLWEYKGIKGIHQARKHMTWYVKGFVGAAELRDRLCRI
NSVEEGLDLLDQTIDGNPTPQT
>Mature_341_residues
SAPSINPAKLRSELSTPLEIGPFAVNSRVLQSPLSGVTDLVFRRLVRRYAPESMLYTEMVHASQVCHARELSKVMDVDLD
EQPISVQLFDCRPHFLAEAAQMAVQEGADTIDLNMGCPVNKITKKGGGSSLLRDPETAEAIVRAVVSAVKVPVTVKTRIG
WKEDEINILEFAQRMQDAGAKMITVHGRTRAQGYNGPAQWHWIKQVKEKLSIPVIANGDIVSVESAMACLEMTGADGVMC
SRGTLGYPYLVGEIDYFLKTGQQKAPPTIIERLQCAREHLQGLWEYKGIKGIHQARKHMTWYVKGFVGAAELRDRLCRIN
SVEEGLDLLDQTIDGNPTPQT

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:
GO:0003824: tRNA-dihydrouridine synthase
GO:0008033: tRNA-dihydrouridine synthase
GO:0008152: tRNA-dihydrouridine synthase
GO:0016491: tRNA-dihydrouridine synthase
GO:0017150: tRNA-dihydrouridine synthase
GO:0050660: tRNA-dihydrouridine synthase
GO:0055114: tRNA-dihydrouridine synthase

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family [H]

Homologues:

Organism=Homo sapiens, GI40807366, Length=305, Percent_Identity=30.4918032786885, Blast_Score=119, Evalue=3e-27,
Organism=Homo sapiens, GI239788483, Length=281, Percent_Identity=28.4697508896797, Blast_Score=116, Evalue=2e-26,
Organism=Homo sapiens, GI31742496, Length=225, Percent_Identity=34.2222222222222, Blast_Score=116, Evalue=3e-26,
Organism=Homo sapiens, GI239788462, Length=242, Percent_Identity=30.1652892561983, Blast_Score=116, Evalue=3e-26,
Organism=Homo sapiens, GI8923374, Length=155, Percent_Identity=36.7741935483871, Blast_Score=86, Evalue=5e-17,
Organism=Escherichia coli, GI1789660, Length=317, Percent_Identity=38.1703470031546, Blast_Score=213, Evalue=1e-56,
Organism=Escherichia coli, GI1788462, Length=306, Percent_Identity=33.0065359477124, Blast_Score=142, Evalue=2e-35,
Organism=Escherichia coli, GI145693211, Length=328, Percent_Identity=26.8292682926829, Blast_Score=96, Evalue=4e-21,
Organism=Caenorhabditis elegans, GI25144369, Length=249, Percent_Identity=32.9317269076305, Blast_Score=116, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI17543114, Length=248, Percent_Identity=31.4516129032258, Blast_Score=105, Evalue=5e-23,
Organism=Caenorhabditis elegans, GI17507177, Length=246, Percent_Identity=30.0813008130081, Blast_Score=103, Evalue=1e-22,
Organism=Saccharomyces cerevisiae, GI6323560, Length=302, Percent_Identity=31.7880794701987, Blast_Score=120, Evalue=3e-28,
Organism=Saccharomyces cerevisiae, GI6323437, Length=257, Percent_Identity=29.1828793774319, Blast_Score=96, Evalue=1e-20,
Organism=Saccharomyces cerevisiae, GI6323433, Length=318, Percent_Identity=26.7295597484277, Blast_Score=93, Evalue=7e-20,
Organism=Drosophila melanogaster, GI24580595, Length=242, Percent_Identity=33.8842975206612, Blast_Score=127, Evalue=8e-30,
Organism=Drosophila melanogaster, GI19920448, Length=242, Percent_Identity=33.8842975206612, Blast_Score=127, Evalue=8e-30,
Organism=Drosophila melanogaster, GI24585320, Length=239, Percent_Identity=33.0543933054393, Blast_Score=112, Evalue=5e-25,
Organism=Drosophila melanogaster, GI19921524, Length=314, Percent_Identity=28.6624203821656, Blast_Score=108, Evalue=5e-24,
Organism=Drosophila melanogaster, GI45549423, Length=147, Percent_Identity=33.3333333333333, Blast_Score=80, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR004652
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: NA

Molecular weight: Translated: 37825; Mature: 37694

Theoretical pI: Translated: 7.19; Mature: 7.19

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAPSINPAKLRSELSTPLEIGPFAVNSRVLQSPLSGVTDLVFRRLVRRYAPESMLYTEM
CCCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
VHASQVCHARELSKVMDVDLDEQPISVQLFDCRPHFLAEAAQMAVQEGADTIDLNMGCPV
HHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCHHHHHHHHHHHHHCCCCEEEECCCCCH
NKITKKGGGSSLLRDPETAEAIVRAVVSAVKVPVTVKTRIGWKEDEINILEFAQRMQDAG
HHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHCCEEEEECCCCCCCCHHHHHHHHHHHHCC
AKMITVHGRTRAQGYNGPAQWHWIKQVKEKLSIPVIANGDIVSVESAMACLEMTGADGVM
CEEEEEECCHHCCCCCCCHHHHHHHHHHHHCCCCEEECCCEEEHHHHHHHHHHCCCCCEE
CSRGTLGYPYLVGEIDYFLKTGQQKAPPTIIERLQCAREHLQGLWEYKGIKGIHQARKHM
ECCCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
TWYVKGFVGAAELRDRLCRINSVEEGLDLLDQTIDGNPTPQT
HHHHHHHCCHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
SAPSINPAKLRSELSTPLEIGPFAVNSRVLQSPLSGVTDLVFRRLVRRYAPESMLYTEM
CCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
VHASQVCHARELSKVMDVDLDEQPISVQLFDCRPHFLAEAAQMAVQEGADTIDLNMGCPV
HHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCHHHHHHHHHHHHHCCCCEEEECCCCCH
NKITKKGGGSSLLRDPETAEAIVRAVVSAVKVPVTVKTRIGWKEDEINILEFAQRMQDAG
HHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHCCEEEEECCCCCCCCHHHHHHHHHHHHCC
AKMITVHGRTRAQGYNGPAQWHWIKQVKEKLSIPVIANGDIVSVESAMACLEMTGADGVM
CEEEEEECCHHCCCCCCCHHHHHHHHHHHHCCCCEEECCCEEEHHHHHHHHHHCCCCCEE
CSRGTLGYPYLVGEIDYFLKTGQQKAPPTIIERLQCAREHLQGLWEYKGIKGIHQARKHM
ECCCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
TWYVKGFVGAAELRDRLCRINSVEEGLDLLDQTIDGNPTPQT
HHHHHHHCCHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]