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The map label for this gene is trpA

Identifier: 158334521

GI number: 158334521

Start: 1324768

End: 1325571

Strand: Reverse

Name: trpA

Synonym: AM1_1347

Alternate gene names: 158334521

Gene position: 1325571-1324768 (Counterclockwise)

Preceding gene: 158334522

Following gene: 158334520

Centisome position: 20.38

GC content: 51.24

Gene sequence:

>804_bases
ATGACCTCTGTTTCTGATTGTTTTGCCCAATTGCGTGATCGCGGCCAGTGCGCCTTAATTCCCTTCTTAACGGCTGGAGA
TCCAGATTTAGCAACCACTGCCAGTGCACTACGCCAACTGGATGCGAGTGGGGCAGATTTGATCGAATTAGGGGTTCCCT
ATTCTGATCCCCTGGCAGATGGTCCCGTAATCCAGGCTGCAGCAACTCGAGCCTTACAGCGGGGGACCCGGCTCGATCAG
GTCTTAGAGATGGTGACAGAGCTTTCCCCCGAAATTCGAGCCCCTATTATTTTATTTACCTACTACAACCCTATCTATCA
TCGCGGTGTTGCAGAGTTTCTGCAGCAGATTGCGAAAGCAGGTGTGCGAGGGTTAGTGGTACCGGATCTTCCCTTGGAAG
AGTCGGAGAACCTCTTGCAGCAAGCGGCTGACTTGGGAATTGAAGTGACATTGCTGGTTGCACCGACTAGCTCTAAAGAA
CGGATTGAGAAAATTGCCTTGCGATCGCAAGGCTTTATCTACCTCGTGAGCACCACTGGGGTCACGGGGATGCGCACCAA
GGTGGAAAACCGCGTCCAAGATTTAATTGCTGATTTACGACAAGTCACAGATAAACCCATCGGCGTTGGTTTTGGCATCT
CTCGAACCGAACATGCTCGCCAAGTCATGGATTGGGGCGCGGATGCAGCGATTGTGGGGAGTGCATTTGTGAATCGGTTG
AGTGAAGGCAGTCCCAGCCAGGGTTTATCTGCAATTAGTACGTTTTGTCGATCCCTTAAATCCTCGCTGCTATTAGACGC
CTAA

Upstream 100 bases:

>100_bases
AGGACTATGAAAATGCCCTGCTGGAAAAGCGCCTGCAAGAAATGACTCCGGAAGAACTCGAAGCCTTACAAGCTTCTATT
GACGATGAGACTGAATAGTC

Downstream 100 bases:

>100_bases
TTCTCCAATGAGATTGCATCGTGCTTGAGGTGTAAATTTTGAGATTGGATGCCACAGGTAGCCCGTGCAAGTCACCAGGT
TTTAGCTATGGGTAAGCTGA

Product: tryptophan synthase subunit alpha

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 267; Mature: 266

Protein sequence:

>267_residues
MTSVSDCFAQLRDRGQCALIPFLTAGDPDLATTASALRQLDASGADLIELGVPYSDPLADGPVIQAAATRALQRGTRLDQ
VLEMVTELSPEIRAPIILFTYYNPIYHRGVAEFLQQIAKAGVRGLVVPDLPLEESENLLQQAADLGIEVTLLVAPTSSKE
RIEKIALRSQGFIYLVSTTGVTGMRTKVENRVQDLIADLRQVTDKPIGVGFGISRTEHARQVMDWGADAAIVGSAFVNRL
SEGSPSQGLSAISTFCRSLKSSLLLDA

Sequences:

>Translated_267_residues
MTSVSDCFAQLRDRGQCALIPFLTAGDPDLATTASALRQLDASGADLIELGVPYSDPLADGPVIQAAATRALQRGTRLDQ
VLEMVTELSPEIRAPIILFTYYNPIYHRGVAEFLQQIAKAGVRGLVVPDLPLEESENLLQQAADLGIEVTLLVAPTSSKE
RIEKIALRSQGFIYLVSTTGVTGMRTKVENRVQDLIADLRQVTDKPIGVGFGISRTEHARQVMDWGADAAIVGSAFVNRL
SEGSPSQGLSAISTFCRSLKSSLLLDA
>Mature_266_residues
TSVSDCFAQLRDRGQCALIPFLTAGDPDLATTASALRQLDASGADLIELGVPYSDPLADGPVIQAAATRALQRGTRLDQV
LEMVTELSPEIRAPIILFTYYNPIYHRGVAEFLQQIAKAGVRGLVVPDLPLEESENLLQQAADLGIEVTLLVAPTSSKER
IEKIALRSQGFIYLVSTTGVTGMRTKVENRVQDLIADLRQVTDKPIGVGFGISRTEHARQVMDWGADAAIVGSAFVNRLS
EGSPSQGLSAISTFCRSLKSSLLLDA

Specific function: The alpha subunit is responsible for the aldol cleavage of indoleglycerol phosphate to indole and glyceraldehyde 3- phosphate

COG id: COG0159

COG function: function code E; Tryptophan synthase alpha chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trpA family

Homologues:

Organism=Escherichia coli, GI1787514, Length=267, Percent_Identity=35.2059925093633, Blast_Score=155, Evalue=2e-39,
Organism=Saccharomyces cerevisiae, GI6321412, Length=232, Percent_Identity=41.8103448275862, Blast_Score=181, Evalue=1e-46,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): TRPA_ACAM1 (B0C6F8)

Other databases:

- EMBL:   CP000828
- RefSeq:   YP_001515693.1
- ProteinModelPortal:   B0C6F8
- SMR:   B0C6F8
- GeneID:   5680168
- GenomeReviews:   CP000828_GR
- KEGG:   amr:AM1_1347
- HOGENOM:   HBG391690
- OMA:   NPIYIYG
- ProtClustDB:   PRK13111
- BioCyc:   AMAR329726:AM1_1347-MONOMER
- HAMAP:   MF_00131
- InterPro:   IPR013785
- InterPro:   IPR011060
- InterPro:   IPR018204
- InterPro:   IPR002028
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00262

Pfam domain/function: PF00290 Trp_syntA; SSF51366 RibP_bind_barrel

EC number: =4.2.1.20

Molecular weight: Translated: 28682; Mature: 28551

Theoretical pI: Translated: 4.72; Mature: 4.72

Prosite motif: PS00167 TRP_SYNTHASE_ALPHA

Important sites: ACT_SITE 49-49 ACT_SITE 60-60

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSVSDCFAQLRDRGQCALIPFLTAGDPDLATTASALRQLDASGADLIELGVPYSDPLAD
CCCHHHHHHHHHCCCCEEEEEEEECCCCCHHHHHHHHHHHCCCCCCEEEECCCCCCCCCC
GPVIQAAATRALQRGTRLDQVLEMVTELSPEIRAPIILFTYYNPIYHRGVAEFLQQIAKA
CCHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHC
GVRGLVVPDLPLEESENLLQQAADLGIEVTLLVAPTSSKERIEKIALRSQGFIYLVSTTG
CCCEEECCCCCCHHHHHHHHHHHHCCCEEEEEEECCCCHHHHHHHHHHCCCEEEEEECCC
VTGMRTKVENRVQDLIADLRQVTDKPIGVGFGISRTEHARQVMDWGADAAIVGSAFVNRL
CCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHH
SEGSPSQGLSAISTFCRSLKSSLLLDA
CCCCCCCHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
TSVSDCFAQLRDRGQCALIPFLTAGDPDLATTASALRQLDASGADLIELGVPYSDPLAD
CCHHHHHHHHHCCCCEEEEEEEECCCCCHHHHHHHHHHHCCCCCCEEEECCCCCCCCCC
GPVIQAAATRALQRGTRLDQVLEMVTELSPEIRAPIILFTYYNPIYHRGVAEFLQQIAKA
CCHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHC
GVRGLVVPDLPLEESENLLQQAADLGIEVTLLVAPTSSKERIEKIALRSQGFIYLVSTTG
CCCEEECCCCCCHHHHHHHHHHHHCCCEEEEEEECCCCHHHHHHHHHHCCCEEEEEECCC
VTGMRTKVENRVQDLIADLRQVTDKPIGVGFGISRTEHARQVMDWGADAAIVGSAFVNRL
CCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHH
SEGSPSQGLSAISTFCRSLKSSLLLDA
CCCCCCCHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA