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The map label for this gene is mgsA [C]

Identifier: 158334485

GI number: 158334485

Start: 1294761

End: 1296053

Strand: Direct

Name: mgsA [C]

Synonym: AM1_1309

Alternate gene names: 158334485

Gene position: 1294761-1296053 (Clockwise)

Preceding gene: 158334484

Following gene: 158334487

Centisome position: 19.91

GC content: 46.33

Gene sequence:

>1293_bases
ATGCCTAATTCTATTGCTTTGATTGCCCATGAAAGCAAGAAAAACGACATCGTTACTCTTGTCCAGCGGTATCATTCGCT
TCTTAACCGCTATCTTATTGTCGCTACTCCAGATACTGGAAAATGGATTCAAGATGCGACTAACCTTACAGTTGAGACTG
TAGTATCAGAAGCAGATGGTGGTCTGTTGCAAATCGCGGCCCGACTAGTATCAAGCGAAGTCGTAGGTGTGATTTTCCTG
ATGGATACGATCAACGTTCCATCTCAGTTACCCGACGTTCAAGCTCTATTGAGGGTTTGTAACCGTTATGAGGTGCCCTT
AGCGACTAACATTGCAACGGCTGAATTGGCGATTCAGGGGGTCGCCAAGCGTCGGGACGCTTATTTGATTTTTAATCCCA
TAGCAGGACAAGGTAATCCAGATCGCGACTTGGCTCTGATTCAGAAAATTCTGGAACCACAGATGAATCTCCACATTATC
TTGACGCAACCAGATATTGATCCAGTAGAGCAAGCTAAGGAAGCAATTACCGCAATCCAATCTGCTCAATCTGAGGACAG
CACTGCTTGTATTATTGCTTCTGGGGGAGATGGAACTGTGTCAGCCATTGCAGGTACCACTCTTGAAACTGGTATTCCTG
TTGGCATTATTCCCAGAGGAACCGCCAACGCCTTTGCCGTGGCTTTAGGATTACCTACAGATCTCAAGCGGGCCTGTGAA
ACGATTGCAGCAGGTAACACGCGTATGGTGGATGCAGCTTACTGTAACGATATCCCAATGGTGCTTTTGGCCGGAGTGGG
GTTTGAAGCTCAAATGGTCAACAACGCCAGCCGAGAATTAAAAAATCGCATAGGCACGCTTGCCTACGTCTTGTCTGCCG
CCCAGCAATTTTTTGCCCAAAAGGATTTCAATGCCACGGTTGAGTTAGATGGTCAGGTTTTGGAATTTAAGACTGGAGCG
GTGACCATTGCCAATGCAGCCCCACCAAGCTCAATTAGTGCCCAGGGGTTTGGAGCTGTAATTCCTGATGATGGGTTGCT
AGAGGTTACCATTCCAACATCCAAAACTCTACTACAGGACATAAATACTTCTGCGACGCTGCTTGCCTCAGCTTTAGTGA
AGACTCAATTTGAAGATAGAAATCTAGTCTGTTTGCGGACCCATCAACTTAAGGTAACGACTGATCCACCGCAAACACTC
GTGGTAGATGGTGAAGTTTTGGAAGCTAATCCCATAGAATTTATCTGCGTCCCCAAAGGGCTAACAGTGTTCTCACCTCT
ATTAACTACTTAG

Upstream 100 bases:

>100_bases
ATCGTTGAATTTTAATAATACTCCTCAACAGATTGAGTCACGACAAGCCTTCGCTAAAGACAAATTAACTGCTCTTTAAT
TTAGACAAGATTAGTTGTCT

Downstream 100 bases:

>100_bases
GTATCCCTCTTTTGTCACTTCAGATAGATGAAATTTGAAACAACGTAATTGCGTTTGGAGTGAACCCAATCATCAGGCCA
TGCACTAACGTAATTTCAAG

Product: putative lipid kinase

Products: Methylglyoxal; Phosphate [C]

Alternate protein names: NA

Number of amino acids: Translated: 430; Mature: 429

Protein sequence:

>430_residues
MPNSIALIAHESKKNDIVTLVQRYHSLLNRYLIVATPDTGKWIQDATNLTVETVVSEADGGLLQIAARLVSSEVVGVIFL
MDTINVPSQLPDVQALLRVCNRYEVPLATNIATAELAIQGVAKRRDAYLIFNPIAGQGNPDRDLALIQKILEPQMNLHII
LTQPDIDPVEQAKEAITAIQSAQSEDSTACIIASGGDGTVSAIAGTTLETGIPVGIIPRGTANAFAVALGLPTDLKRACE
TIAAGNTRMVDAAYCNDIPMVLLAGVGFEAQMVNNASRELKNRIGTLAYVLSAAQQFFAQKDFNATVELDGQVLEFKTGA
VTIANAAPPSSISAQGFGAVIPDDGLLEVTIPTSKTLLQDINTSATLLASALVKTQFEDRNLVCLRTHQLKVTTDPPQTL
VVDGEVLEANPIEFICVPKGLTVFSPLLTT

Sequences:

>Translated_430_residues
MPNSIALIAHESKKNDIVTLVQRYHSLLNRYLIVATPDTGKWIQDATNLTVETVVSEADGGLLQIAARLVSSEVVGVIFL
MDTINVPSQLPDVQALLRVCNRYEVPLATNIATAELAIQGVAKRRDAYLIFNPIAGQGNPDRDLALIQKILEPQMNLHII
LTQPDIDPVEQAKEAITAIQSAQSEDSTACIIASGGDGTVSAIAGTTLETGIPVGIIPRGTANAFAVALGLPTDLKRACE
TIAAGNTRMVDAAYCNDIPMVLLAGVGFEAQMVNNASRELKNRIGTLAYVLSAAQQFFAQKDFNATVELDGQVLEFKTGA
VTIANAAPPSSISAQGFGAVIPDDGLLEVTIPTSKTLLQDINTSATLLASALVKTQFEDRNLVCLRTHQLKVTTDPPQTL
VVDGEVLEANPIEFICVPKGLTVFSPLLTT
>Mature_429_residues
PNSIALIAHESKKNDIVTLVQRYHSLLNRYLIVATPDTGKWIQDATNLTVETVVSEADGGLLQIAARLVSSEVVGVIFLM
DTINVPSQLPDVQALLRVCNRYEVPLATNIATAELAIQGVAKRRDAYLIFNPIAGQGNPDRDLALIQKILEPQMNLHIIL
TQPDIDPVEQAKEAITAIQSAQSEDSTACIIASGGDGTVSAIAGTTLETGIPVGIIPRGTANAFAVALGLPTDLKRACET
IAAGNTRMVDAAYCNDIPMVLLAGVGFEAQMVNNASRELKNRIGTLAYVLSAAQQFFAQKDFNATVELDGQVLEFKTGAV
TIANAAPPSSISAQGFGAVIPDDGLLEVTIPTSKTLLQDINTSATLLASALVKTQFEDRNLVCLRTHQLKVTTDPPQTLV
VDGEVLEANPIEFICVPKGLTVFSPLLTT

Specific function: Unknown

COG id: COG1597

COG function: function code IR; Sphingosine kinase and enzymes related to eukaryotic diacylglycerol kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 DAGKc domain [H]

Homologues:

Organism=Escherichia coli, GI87081809, Length=117, Percent_Identity=36.7521367521368, Blast_Score=82, Evalue=5e-17,
Organism=Escherichia coli, GI1788402, Length=310, Percent_Identity=23.5483870967742, Blast_Score=65, Evalue=6e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001206
- InterPro:   IPR004363
- InterPro:   IPR018148
- InterPro:   IPR011607 [H]

Pfam domain/function: PF00781 DAGK_cat; PF02142 MGS [H]

EC number: 4.2.3.3 [C]

Molecular weight: Translated: 45717; Mature: 45586

Theoretical pI: Translated: 4.44; Mature: 4.44

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPNSIALIAHESKKNDIVTLVQRYHSLLNRYLIVATPDTGKWIQDATNLTVETVVSEADG
CCCCEEEEEECCCCCHHHHHHHHHHHHHCCEEEEEECCCCCHHHHHCCCEEEEHHHHCCC
GLLQIAARLVSSEVVGVIFLMDTINVPSQLPDVQALLRVCNRYEVPLATNIATAELAIQG
CHHHHHHHHHHHHHHEEEEEEECCCCCCCCCCHHHHHHHHCCCCCCEECCCHHHHHHHHH
VAKRRDAYLIFNPIAGQGNPDRDLALIQKILEPQMNLHIILTQPDIDPVEQAKEAITAIQ
HHHCCCEEEEEECCCCCCCCCHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHH
SAQSEDSTACIIASGGDGTVSAIAGTTLETGIPVGIIPRGTANAFAVALGLPTDLKRACE
HCCCCCCCEEEEEECCCCEEEEEECCCHHCCCCEEEECCCCCCEEEEEECCCHHHHHHHH
TIAAGNTRMVDAAYCNDIPMVLLAGVGFEAQMVNNASRELKNRIGTLAYVLSAAQQFFAQ
HHHCCCCEEEEHHHHCCCCEEEHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KDFNATVELDGQVLEFKTGAVTIANAAPPSSISAQGFGAVIPDDGLLEVTIPTSKTLLQD
CCCCEEEEECCEEEEEECCEEEEECCCCCCCCCCCCCCEECCCCCEEEEEECCHHHHHHH
INTSATLLASALVKTQFEDRNLVCLRTHQLKVTTDPPQTLVVDGEVLEANPIEFICVPKG
CCCHHHHHHHHHHHHCCCCCCEEEEEEEEEEEECCCCCEEEECCEEEECCCEEEEEECCC
LTVFSPLLTT
CHHHHHHHCC
>Mature Secondary Structure 
PNSIALIAHESKKNDIVTLVQRYHSLLNRYLIVATPDTGKWIQDATNLTVETVVSEADG
CCCEEEEEECCCCCHHHHHHHHHHHHHCCEEEEEECCCCCHHHHHCCCEEEEHHHHCCC
GLLQIAARLVSSEVVGVIFLMDTINVPSQLPDVQALLRVCNRYEVPLATNIATAELAIQG
CHHHHHHHHHHHHHHEEEEEEECCCCCCCCCCHHHHHHHHCCCCCCEECCCHHHHHHHHH
VAKRRDAYLIFNPIAGQGNPDRDLALIQKILEPQMNLHIILTQPDIDPVEQAKEAITAIQ
HHHCCCEEEEEECCCCCCCCCHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHH
SAQSEDSTACIIASGGDGTVSAIAGTTLETGIPVGIIPRGTANAFAVALGLPTDLKRACE
HCCCCCCCEEEEEECCCCEEEEEECCCHHCCCCEEEECCCCCCEEEEEECCCHHHHHHHH
TIAAGNTRMVDAAYCNDIPMVLLAGVGFEAQMVNNASRELKNRIGTLAYVLSAAQQFFAQ
HHHCCCCEEEEHHHHCCCCEEEHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KDFNATVELDGQVLEFKTGAVTIANAAPPSSISAQGFGAVIPDDGLLEVTIPTSKTLLQD
CCCCEEEEECCEEEEEECCEEEEECCCCCCCCCCCCCCEECCCCCEEEEEECCHHHHHHH
INTSATLLASALVKTQFEDRNLVCLRTHQLKVTTDPPQTLVVDGEVLEANPIEFICVPKG
CCCHHHHHHHHHHHHCCCCCCEEEEEEEEEEEECCCCCEEEECCEEEECCCEEEEEECCC
LTVFSPLLTT
CHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): 13200 [C]

Specific activity: NA

Km value (mM): 0.5 {dihydroxyacetone} 0.47 {dihydroxyacetone} 0.2 {dihydroxyacetone} [C]

Substrates: Dihydroxyacetone phosphate [C]

Specific reaction: Dihydroxyacetone phosphate --> Methylglyoxal + Phosphate [C]

General reaction: elimination (C-O bond cleavage [C]

Inhibitor: 2-phosphoglycerate; 3-phosphoglycerate; arsenate; diphosphate; phosphate; phosphoenolpyruvate [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]