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The map label for this gene is 158334451

Identifier: 158334451

GI number: 158334451

Start: 1260067

End: 1261506

Strand: Direct

Name: 158334451

Synonym: AM1_1272

Alternate gene names: NA

Gene position: 1260067-1261506 (Clockwise)

Preceding gene: 158334449

Following gene: 158334452

Centisome position: 19.37

GC content: 52.5

Gene sequence:

>1440_bases
ATGATCACCCAGTCTCATCCTCAACAATTTCAGCAACCTTGGTATGGCATGTTTGCCCAAGCTGCACCCGAATTTCCGAC
CACCCGGTTACCGATTTTGTCCGGGGCTATACCCGAAAACCTCAAAGGCTCTCTATATCGCAACGGCCCCGGTCGCCTAA
AGCGCGGCGGACAACGGGTTGGCCACTGGTTTGATGGGGATGGAGCCGTTTTAGCCGTGCATTTCGATAGCGTCAATGCA
ACAGGCGTCTATCGCTATGTTCAAACCGCAGGCTATCAGGCCGAAGAACAGGCCGATCAATTCTTGATGGGAGGTTATGG
CATGCTGCCCGCCGGATCCCTGTGGCAAAGATTGCGGCAGGGAGCAACCAAAAATGCTGCCAATACCTCTGTGCTTGCCC
TCCCCGACAAGCTGTTGACCTTGTGGGAAGGCGGACATCCTCATGCTCTGGACTTAAACACTTTGGAGACCCAAAGGCTA
GACGATTTGGGATTCCTAGCACCGGATCAGTCTTTCTCCGCCCATCCCAAATGGGATCCGATCAGCAGCGAAATCTATAA
CTTTGGCGTTAGCTATAGCAAGCAAGGTCATCTGCATGTCTATCGCTGCAATCAAGAAGGAGCCGTTCAGCGCCAAACCA
AAATTCCGCTCCAGGGCTTGCCGATGATTCATGACTTTGTGATGGCGGGGCCTTACTTAATTTTTTGTATTTCCCCCGTC
TTCCTCAATCCACTGCCTATGTTGGCCCAGCTCAAAAGCTATAGCGACAGTCTCCAATGGAAACCCCAGCAAGGCACTCA
TATCTTAGTGATTGATCGTGAGTCCCTAGAAGTGGTCAGCCAAGGTGAAGCAGATCCCTGGTATCAGTGGCATTTTGGTA
ATGGCTATGTGGACGACCAAGAAAATGTCGTTATCGATGTCGTCCGTTATGCCGACTTCAGCACCAACCAGTTCCTCAAA
GAAGTAGCCAGTGGTCATCCCCACACGCAAGCAGAAGGACAGCTTTGGCGACTACATCTCGATCCCCAGACGAGCCAAGT
GTTGGAGACCTATAAGCTGATGGACCGCACAGGCGAGTTTCCAGTGGTTGATCCCCATCAGGTGGGACAGGCCCATCGGC
ATACCTATCTCTCCACTCGCCCCACCTCAGACCCTACAACAGAACTCTTTCGTGAGATTGTCAGCTTCGATCACGTCTCG
CAACAACTCAGCAGTGTGCAGATGGGCGATCAAGCCTATTGCAGTGAGCCCATCTATGCTCCTGATCCCGATCACCCTGG
ACAGGGCTGGATTCTGACCGTGGTCTATCAAGCTGCATCCCATACTAGTGAGGTGTGGATTTTGGCCGCCCATCGTCTTG
AAGAAGGCCCGATTTGCCGATTAGGATTACCTAGCGTCGTCCCCATGGGGTTTCATGGCACTTGGCAGCCGAGTTTGTAA

Upstream 100 bases:

>100_bases
GACTCATATGCACTGATTGACATATTCAAATAATTGCATATAGTGAGAATATGCACTTTGTTGCATATTACATCGTCTGC
GTTGCCTAGGAGACCCACCT

Downstream 100 bases:

>100_bases
CCCTCATCAAGCAAGGGGAGAAGGAAGCAAAGTACAATGCTACACACCTCAGCAATTCATCCTTTCGCCAAACGTTTGTG
AGATAGTAACTGAGGCCCGC

Product: hypothetical protein

Products: NA

Alternate protein names: ACO; 8'-apo-beta-carotenal 15,15'-oxygenase; Diox1 [H]

Number of amino acids: Translated: 479; Mature: 479

Protein sequence:

>479_residues
MITQSHPQQFQQPWYGMFAQAAPEFPTTRLPILSGAIPENLKGSLYRNGPGRLKRGGQRVGHWFDGDGAVLAVHFDSVNA
TGVYRYVQTAGYQAEEQADQFLMGGYGMLPAGSLWQRLRQGATKNAANTSVLALPDKLLTLWEGGHPHALDLNTLETQRL
DDLGFLAPDQSFSAHPKWDPISSEIYNFGVSYSKQGHLHVYRCNQEGAVQRQTKIPLQGLPMIHDFVMAGPYLIFCISPV
FLNPLPMLAQLKSYSDSLQWKPQQGTHILVIDRESLEVVSQGEADPWYQWHFGNGYVDDQENVVIDVVRYADFSTNQFLK
EVASGHPHTQAEGQLWRLHLDPQTSQVLETYKLMDRTGEFPVVDPHQVGQAHRHTYLSTRPTSDPTTELFREIVSFDHVS
QQLSSVQMGDQAYCSEPIYAPDPDHPGQGWILTVVYQAASHTSEVWILAAHRLEEGPICRLGLPSVVPMGFHGTWQPSL

Sequences:

>Translated_479_residues
MITQSHPQQFQQPWYGMFAQAAPEFPTTRLPILSGAIPENLKGSLYRNGPGRLKRGGQRVGHWFDGDGAVLAVHFDSVNA
TGVYRYVQTAGYQAEEQADQFLMGGYGMLPAGSLWQRLRQGATKNAANTSVLALPDKLLTLWEGGHPHALDLNTLETQRL
DDLGFLAPDQSFSAHPKWDPISSEIYNFGVSYSKQGHLHVYRCNQEGAVQRQTKIPLQGLPMIHDFVMAGPYLIFCISPV
FLNPLPMLAQLKSYSDSLQWKPQQGTHILVIDRESLEVVSQGEADPWYQWHFGNGYVDDQENVVIDVVRYADFSTNQFLK
EVASGHPHTQAEGQLWRLHLDPQTSQVLETYKLMDRTGEFPVVDPHQVGQAHRHTYLSTRPTSDPTTELFREIVSFDHVS
QQLSSVQMGDQAYCSEPIYAPDPDHPGQGWILTVVYQAASHTSEVWILAAHRLEEGPICRLGLPSVVPMGFHGTWQPSL
>Mature_479_residues
MITQSHPQQFQQPWYGMFAQAAPEFPTTRLPILSGAIPENLKGSLYRNGPGRLKRGGQRVGHWFDGDGAVLAVHFDSVNA
TGVYRYVQTAGYQAEEQADQFLMGGYGMLPAGSLWQRLRQGATKNAANTSVLALPDKLLTLWEGGHPHALDLNTLETQRL
DDLGFLAPDQSFSAHPKWDPISSEIYNFGVSYSKQGHLHVYRCNQEGAVQRQTKIPLQGLPMIHDFVMAGPYLIFCISPV
FLNPLPMLAQLKSYSDSLQWKPQQGTHILVIDRESLEVVSQGEADPWYQWHFGNGYVDDQENVVIDVVRYADFSTNQFLK
EVASGHPHTQAEGQLWRLHLDPQTSQVLETYKLMDRTGEFPVVDPHQVGQAHRHTYLSTRPTSDPTTELFREIVSFDHVS
QQLSSVQMGDQAYCSEPIYAPDPDHPGQGWILTVVYQAASHTSEVWILAAHRLEEGPICRLGLPSVVPMGFHGTWQPSL

Specific function: Cleaves a number of carotenals and carotenols in the all-trans configuration at the 15-15' double bond producing retinal or retinol, respectively. Also shows activity toward lycopenals and the corresponding alcohols. Does not cleave beta- carotene or lyco

COG id: COG3670

COG function: function code Q; Lignostilbene-alpha,beta-dioxygenase and related enzymes

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the carotenoid oxygenase family [H]

Homologues:

Organism=Homo sapiens, GI82617624, Length=528, Percent_Identity=24.2424242424242, Blast_Score=102, Evalue=1e-21,
Organism=Homo sapiens, GI82617628, Length=528, Percent_Identity=24.2424242424242, Blast_Score=102, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI71998242, Length=304, Percent_Identity=24.6710526315789, Blast_Score=96, Evalue=4e-20,
Organism=Caenorhabditis elegans, GI32564756, Length=309, Percent_Identity=22.3300970873786, Blast_Score=89, Evalue=7e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004294 [H]

Pfam domain/function: PF03055 RPE65 [H]

EC number: =1.14.99.41 [H]

Molecular weight: Translated: 53664; Mature: 53664

Theoretical pI: Translated: 6.02; Mature: 6.02

Prosite motif: PS00678 WD_REPEATS_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MITQSHPQQFQQPWYGMFAQAAPEFPTTRLPILSGAIPENLKGSLYRNGPGRLKRGGQRV
CCCCCCCHHHHCHHHHHHHHCCCCCCCCCCCHHCCCCCCCCCCCEEECCCHHHHCCCHHC
GHWFDGDGAVLAVHFDSVNATGVYRYVQTAGYQAEEQADQFLMGGYGMLPAGSLWQRLRQ
CCEECCCCEEEEEEECCCCCCHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHC
GATKNAANTSVLALPDKLLTLWEGGHPHALDLNTLETQRLDDLGFLAPDQSFSAHPKWDP
CCCCCCCCCEEEECCHHHHHHHCCCCCCEEECCCHHHHHHCCCCCCCCCCCCCCCCCCCC
ISSEIYNFGVSYSKQGHLHVYRCNQEGAVQRQTKIPLQGLPMIHDFVMAGPYLIFCISPV
CHHHHHHCCCCCCCCCCEEEEEECCCCCCHHHHCCCCCCCCHHHHHHHHCCEEEEECCHH
FLNPLPMLAQLKSYSDSLQWKPQQGTHILVIDRESLEVVSQGEADPWYQWHFGNGYVDDQ
HHCCHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCHHHHHCCCCCCCEEEECCCCEECCC
ENVVIDVVRYADFSTNQFLKEVASGHPHTQAEGQLWRLHLDPQTSQVLETYKLMDRTGEF
CCEEEEEEECCCCCHHHHHHHHHCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHCCCCC
PVVDPHQVGQAHRHTYLSTRPTSDPTTELFREIVSFDHVSQQLSSVQMGDQAYCSEPIYA
CCCCHHHHCHHHHCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCC
PDPDHPGQGWILTVVYQAASHTSEVWILAAHRLEEGPICRLGLPSVVPMGFHGTWQPSL
CCCCCCCCCEEEEEEEHHHCCCCEEEEEEEECCCCCCEEEECCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MITQSHPQQFQQPWYGMFAQAAPEFPTTRLPILSGAIPENLKGSLYRNGPGRLKRGGQRV
CCCCCCCHHHHCHHHHHHHHCCCCCCCCCCCHHCCCCCCCCCCCEEECCCHHHHCCCHHC
GHWFDGDGAVLAVHFDSVNATGVYRYVQTAGYQAEEQADQFLMGGYGMLPAGSLWQRLRQ
CCEECCCCEEEEEEECCCCCCHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHC
GATKNAANTSVLALPDKLLTLWEGGHPHALDLNTLETQRLDDLGFLAPDQSFSAHPKWDP
CCCCCCCCCEEEECCHHHHHHHCCCCCCEEECCCHHHHHHCCCCCCCCCCCCCCCCCCCC
ISSEIYNFGVSYSKQGHLHVYRCNQEGAVQRQTKIPLQGLPMIHDFVMAGPYLIFCISPV
CHHHHHHCCCCCCCCCCEEEEEECCCCCCHHHHCCCCCCCCHHHHHHHHCCEEEEECCHH
FLNPLPMLAQLKSYSDSLQWKPQQGTHILVIDRESLEVVSQGEADPWYQWHFGNGYVDDQ
HHCCHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCHHHHHCCCCCCCEEEECCCCEECCC
ENVVIDVVRYADFSTNQFLKEVASGHPHTQAEGQLWRLHLDPQTSQVLETYKLMDRTGEF
CCEEEEEEECCCCCHHHHHHHHHCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHCCCCC
PVVDPHQVGQAHRHTYLSTRPTSDPTTELFREIVSFDHVSQQLSSVQMGDQAYCSEPIYA
CCCCHHHHCHHHHCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCC
PDPDHPGQGWILTVVYQAASHTSEVWILAAHRLEEGPICRLGLPSVVPMGFHGTWQPSL
CCCCCCCCCEEEEEEEHHHCCCCEEEEEEEECCCCCCEEEECCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]