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The map label for this gene is menE [H]

Identifier: 158334422

GI number: 158334422

Start: 1228024

End: 1229529

Strand: Reverse

Name: menE [H]

Synonym: AM1_1243

Alternate gene names: 158334422

Gene position: 1229529-1228024 (Counterclockwise)

Preceding gene: 158334423

Following gene: 158334420

Centisome position: 18.9

GC content: 52.32

Gene sequence:

>1506_bases
ATGATCTTTACTCTGCTGAGAAAACTGCATCGGACGCGCCTATTAACCTTGGTTGGTTTGTATCGTTTGCTGGAAGCGAT
CTGGACGACGGGGGTTAACTTGATGGCTCTGTTGCGAATTGCGGCCAAGCTGCATCCTGAGCGGATTGCTGTGATTGATG
ACCAGGAAGAATTAACCTACTCCAAGTTATGGAAGCAAGCAGAGGCCGTTGCGGTCGGATTGCAGAGAGACTGTGGAATT
CAGCCTCGACAGAGGGTTGCGATCGCAACCCGTAACCATGCCGCAGCCATCAAAGCCATTTTTGCCGTCTCTCGGTTAGG
GGCAGATTTGTTTCTGGTCAATCCCGAAATGAGTGCGGATCAGATCCGGGCCCTGGAACATCAGCTTCAGTTTGACTACT
ACATTTATGATCCAGAACTTACTCCGATCGTTGATGACCTTTCTCTAGGCCATAAGGCCATTCCCACCTATCATCCTTCC
AGCAATTCTGTGGATCGGATGTCTGTGATGCCTCCATCCGCCACTCCCCTGCGTAAGGTGAAGACCGGGAAAGTAGTGGT
GATGACGGGGGGCACGACGGGCCAACCGAAACCGGCCAGCCGTAAACCCTCCCTATTTGATTTCCTCCCTCCCTTTATTG
CCCTACTTTCGCAGATTCATTTGGATCGGTATCGCACGGTGTATATTGCCACCCCGATTTATCATGGCTTTGGGCTAGCC
GCTCTTTTAATTGGGGTGACTTTGGGGGCGCAACAGTATTTCACCCGCCGATTTGATGCCAGTCGCGCCTGTGACCTGAT
TGCCCAAGACAACATTGAGGTGATTACTCTAGTACCGTTGATGTTGCGGCGGATGCTCAACCAAAATGCCCAGGCTTTGT
CTTCCCTCCAGCGTATCCTCACGGGAGGGGCGGCTCTGCGACCCGCGTTGGCCCAAGCCACCCTAGAGCAGTTTGGTCCG
GTTTTGTTTAACCTGTATGGCACGTCTGAAGCAGGGTTTTGTATCTTGGCGGATCCAGAGTTGTTGCAAAAGCAGCCAGA
GTCCATTGGCAAGCCGATTACCGGGGTTCAGGTCCAGATCCAGCCGGATGGACCTTCGTCAAAAGGGCAGCTTTGTATCC
GCAGTGCCTGGTCTGCGGGGGCTAAAGGGTGGATAGAAACGGGAGATTTGGCTTATCGAGATGGGGAAGGGAATCTCTTT
TTGTGTGGCCGGGTGGATGACATGATTGTGTCTGGGGGGGAGAATGTTTATCCCCTGGAACTGGAAAATGTGCTGCTCCA
ACATCCAGGAATTGAGGCGGCAGTGGTCCTGGGGATTCCTGATCCAGAGTTTGGACAGCGATTGAAAGCGATTATTATCC
CTAAGTCTGCTGAGAACTTAGAGCAATCAACGGTATTGGCATGGCTAAAGCCACGGGTGGCTCGCTATCAAATGCCTGCT
GTGATTGAGTTTCGCCAAGCCCTTCCCTATACGTCTCTGGGTAAGTTGAACCGCAAAGCCTTGTGA

Upstream 100 bases:

>100_bases
GCCCCTTGGTGGTTGATGATGGGGGAGTTGGTATCTGTCCTGCTGCGATGGCCGGGAGAAGTAATTTGGAGTGGGGTGAT
GAGGTGGAGACGATAGAGCC

Downstream 100 bases:

>100_bases
ACTGATAGGGTTTGGGAAGTCTCCAGATATCTCAAAGCTCGTATGGGCTGTTGTATAGACCCATACGAGCTATAAAAACG
GTTGACATTGCCCAAGGCTG

Product: 2-succinylbenzoate--CoA ligase

Products: NA

Alternate protein names: OSB-CoA synthetase; o-succinylbenzoyl-CoA synthetase [H]

Number of amino acids: Translated: 501; Mature: 501

Protein sequence:

>501_residues
MIFTLLRKLHRTRLLTLVGLYRLLEAIWTTGVNLMALLRIAAKLHPERIAVIDDQEELTYSKLWKQAEAVAVGLQRDCGI
QPRQRVAIATRNHAAAIKAIFAVSRLGADLFLVNPEMSADQIRALEHQLQFDYYIYDPELTPIVDDLSLGHKAIPTYHPS
SNSVDRMSVMPPSATPLRKVKTGKVVVMTGGTTGQPKPASRKPSLFDFLPPFIALLSQIHLDRYRTVYIATPIYHGFGLA
ALLIGVTLGAQQYFTRRFDASRACDLIAQDNIEVITLVPLMLRRMLNQNAQALSSLQRILTGGAALRPALAQATLEQFGP
VLFNLYGTSEAGFCILADPELLQKQPESIGKPITGVQVQIQPDGPSSKGQLCIRSAWSAGAKGWIETGDLAYRDGEGNLF
LCGRVDDMIVSGGENVYPLELENVLLQHPGIEAAVVLGIPDPEFGQRLKAIIIPKSAENLEQSTVLAWLKPRVARYQMPA
VIEFRQALPYTSLGKLNRKAL

Sequences:

>Translated_501_residues
MIFTLLRKLHRTRLLTLVGLYRLLEAIWTTGVNLMALLRIAAKLHPERIAVIDDQEELTYSKLWKQAEAVAVGLQRDCGI
QPRQRVAIATRNHAAAIKAIFAVSRLGADLFLVNPEMSADQIRALEHQLQFDYYIYDPELTPIVDDLSLGHKAIPTYHPS
SNSVDRMSVMPPSATPLRKVKTGKVVVMTGGTTGQPKPASRKPSLFDFLPPFIALLSQIHLDRYRTVYIATPIYHGFGLA
ALLIGVTLGAQQYFTRRFDASRACDLIAQDNIEVITLVPLMLRRMLNQNAQALSSLQRILTGGAALRPALAQATLEQFGP
VLFNLYGTSEAGFCILADPELLQKQPESIGKPITGVQVQIQPDGPSSKGQLCIRSAWSAGAKGWIETGDLAYRDGEGNLF
LCGRVDDMIVSGGENVYPLELENVLLQHPGIEAAVVLGIPDPEFGQRLKAIIIPKSAENLEQSTVLAWLKPRVARYQMPA
VIEFRQALPYTSLGKLNRKAL
>Mature_501_residues
MIFTLLRKLHRTRLLTLVGLYRLLEAIWTTGVNLMALLRIAAKLHPERIAVIDDQEELTYSKLWKQAEAVAVGLQRDCGI
QPRQRVAIATRNHAAAIKAIFAVSRLGADLFLVNPEMSADQIRALEHQLQFDYYIYDPELTPIVDDLSLGHKAIPTYHPS
SNSVDRMSVMPPSATPLRKVKTGKVVVMTGGTTGQPKPASRKPSLFDFLPPFIALLSQIHLDRYRTVYIATPIYHGFGLA
ALLIGVTLGAQQYFTRRFDASRACDLIAQDNIEVITLVPLMLRRMLNQNAQALSSLQRILTGGAALRPALAQATLEQFGP
VLFNLYGTSEAGFCILADPELLQKQPESIGKPITGVQVQIQPDGPSSKGQLCIRSAWSAGAKGWIETGDLAYRDGEGNLF
LCGRVDDMIVSGGENVYPLELENVLLQHPGIEAAVVLGIPDPEFGQRLKAIIIPKSAENLEQSTVLAWLKPRVARYQMPA
VIEFRQALPYTSLGKLNRKAL

Specific function: Converts 2-succinylbenzoate (OSB) to 2-succinylbenzoyl- CoA (OSB-CoA) [H]

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Partially Membrane-Associated [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family. MenE subfamily [H]

Homologues:

Organism=Homo sapiens, GI156151445, Length=532, Percent_Identity=24.6240601503759, Blast_Score=112, Evalue=8e-25,
Organism=Homo sapiens, GI187761345, Length=525, Percent_Identity=22.4761904761905, Blast_Score=108, Evalue=9e-24,
Organism=Homo sapiens, GI187761343, Length=525, Percent_Identity=22.4761904761905, Blast_Score=108, Evalue=9e-24,
Organism=Homo sapiens, GI38505220, Length=491, Percent_Identity=24.6435845213849, Blast_Score=99, Evalue=1e-20,
Organism=Homo sapiens, GI122937307, Length=361, Percent_Identity=27.7008310249307, Blast_Score=97, Evalue=5e-20,
Organism=Homo sapiens, GI157311624, Length=487, Percent_Identity=24.6406570841889, Blast_Score=94, Evalue=3e-19,
Organism=Homo sapiens, GI157311622, Length=487, Percent_Identity=24.6406570841889, Blast_Score=94, Evalue=3e-19,
Organism=Homo sapiens, GI42544132, Length=352, Percent_Identity=25, Blast_Score=91, Evalue=2e-18,
Organism=Homo sapiens, GI115511026, Length=394, Percent_Identity=26.1421319796954, Blast_Score=88, Evalue=2e-17,
Organism=Homo sapiens, GI58082049, Length=487, Percent_Identity=24.0246406570842, Blast_Score=86, Evalue=6e-17,
Organism=Escherichia coli, GI1788107, Length=534, Percent_Identity=26.0299625468165, Blast_Score=143, Evalue=2e-35,
Organism=Escherichia coli, GI221142682, Length=463, Percent_Identity=23.3261339092873, Blast_Score=112, Evalue=7e-26,
Organism=Escherichia coli, GI145693145, Length=505, Percent_Identity=24.5544554455446, Blast_Score=103, Evalue=3e-23,
Organism=Escherichia coli, GI1786810, Length=500, Percent_Identity=25.4, Blast_Score=99, Evalue=8e-22,
Organism=Escherichia coli, GI1790505, Length=276, Percent_Identity=24.2753623188406, Blast_Score=84, Evalue=3e-17,
Organism=Escherichia coli, GI1788595, Length=304, Percent_Identity=26.9736842105263, Blast_Score=83, Evalue=5e-17,
Organism=Escherichia coli, GI1786529, Length=246, Percent_Identity=26.8292682926829, Blast_Score=74, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17558820, Length=492, Percent_Identity=25.609756097561, Blast_Score=124, Evalue=8e-29,
Organism=Caenorhabditis elegans, GI32563687, Length=484, Percent_Identity=23.7603305785124, Blast_Score=117, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI17531443, Length=357, Percent_Identity=27.4509803921569, Blast_Score=115, Evalue=4e-26,
Organism=Caenorhabditis elegans, GI17559526, Length=354, Percent_Identity=25.9887005649718, Blast_Score=109, Evalue=4e-24,
Organism=Caenorhabditis elegans, GI71994703, Length=533, Percent_Identity=23.827392120075, Blast_Score=108, Evalue=5e-24,
Organism=Caenorhabditis elegans, GI71994694, Length=533, Percent_Identity=23.827392120075, Blast_Score=108, Evalue=6e-24,
Organism=Caenorhabditis elegans, GI71994690, Length=533, Percent_Identity=23.827392120075, Blast_Score=108, Evalue=7e-24,
Organism=Caenorhabditis elegans, GI17560140, Length=494, Percent_Identity=22.2672064777328, Blast_Score=100, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI17557194, Length=300, Percent_Identity=26.6666666666667, Blast_Score=95, Evalue=9e-20,
Organism=Caenorhabditis elegans, GI17538037, Length=295, Percent_Identity=26.7796610169492, Blast_Score=91, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17560308, Length=364, Percent_Identity=26.0989010989011, Blast_Score=84, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI71996755, Length=446, Percent_Identity=25.1121076233184, Blast_Score=83, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI25141359, Length=351, Percent_Identity=26.4957264957265, Blast_Score=79, Evalue=6e-15,
Organism=Caenorhabditis elegans, GI71985884, Length=363, Percent_Identity=22.5895316804408, Blast_Score=69, Evalue=5e-12,
Organism=Saccharomyces cerevisiae, GI6319699, Length=344, Percent_Identity=26.7441860465116, Blast_Score=119, Evalue=2e-27,
Organism=Drosophila melanogaster, GI24581924, Length=529, Percent_Identity=21.7391304347826, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI21355181, Length=346, Percent_Identity=24.5664739884393, Blast_Score=98, Evalue=2e-20,
Organism=Drosophila melanogaster, GI21358303, Length=243, Percent_Identity=26.3374485596708, Blast_Score=93, Evalue=5e-19,
Organism=Drosophila melanogaster, GI281365686, Length=319, Percent_Identity=24.1379310344828, Blast_Score=89, Evalue=6e-18,
Organism=Drosophila melanogaster, GI18859661, Length=303, Percent_Identity=25.4125412541254, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI21356947, Length=503, Percent_Identity=21.4711729622266, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI20130357, Length=347, Percent_Identity=23.6311239193084, Blast_Score=85, Evalue=9e-17,
Organism=Drosophila melanogaster, GI24648257, Length=498, Percent_Identity=24.0963855421687, Blast_Score=84, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24653035, Length=341, Percent_Identity=21.9941348973607, Blast_Score=79, Evalue=1e-14,
Organism=Drosophila melanogaster, GI62472339, Length=122, Percent_Identity=36.0655737704918, Blast_Score=77, Evalue=3e-14,
Organism=Drosophila melanogaster, GI24667959, Length=122, Percent_Identity=36.0655737704918, Blast_Score=76, Evalue=4e-14,
Organism=Drosophila melanogaster, GI24667955, Length=122, Percent_Identity=36.0655737704918, Blast_Score=76, Evalue=4e-14,
Organism=Drosophila melanogaster, GI24648260, Length=344, Percent_Identity=23.8372093023256, Blast_Score=76, Evalue=5e-14,
Organism=Drosophila melanogaster, GI24648255, Length=391, Percent_Identity=24.2966751918159, Blast_Score=75, Evalue=8e-14,
Organism=Drosophila melanogaster, GI24656500, Length=555, Percent_Identity=21.981981981982, Blast_Score=75, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24648253, Length=391, Percent_Identity=24.0409207161125, Blast_Score=73, Evalue=5e-13,
Organism=Drosophila melanogaster, GI21356441, Length=293, Percent_Identity=24.2320819112628, Blast_Score=72, Evalue=1e-12,
Organism=Drosophila melanogaster, GI161076582, Length=347, Percent_Identity=21.3256484149856, Blast_Score=69, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000873
- InterPro:   IPR010192
- InterPro:   IPR023015 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.26 [H]

Molecular weight: Translated: 55227; Mature: 55227

Theoretical pI: Translated: 9.18; Mature: 9.18

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIFTLLRKLHRTRLLTLVGLYRLLEAIWTTGVNLMALLRIAAKLHPERIAVIDDQEELTY
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCEEEEECCHHHHHH
SKLWKQAEAVAVGLQRDCGIQPRQRVAIATRNHAAAIKAIFAVSRLGADLFLVNPEMSAD
HHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCHHHHHHHHHHHHHCCCEEEEECCCCCHH
QIRALEHQLQFDYYIYDPELTPIVDDLSLGHKAIPTYHPSSNSVDRMSVMPPSATPLRKV
HHHHHHHHHEEEEEEECCCCCCHHHHHCCCCCCCCCCCCCCCCCCEEEECCCCCCCHHHC
KTGKVVVMTGGTTGQPKPASRKPSLFDFLPPFIALLSQIHLDRYRTVYIATPIYHGFGLA
CCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCEEEEEECCHHHHHHHH
ALLIGVTLGAQQYFTRRFDASRACDLIAQDNIEVITLVPLMLRRMLNQNAQALSSLQRIL
HHHHHHHHCHHHHHHHHCCCHHHHHHHHCCCCEEEHHHHHHHHHHHCCCHHHHHHHHHHH
TGGAALRPALAQATLEQFGPVLFNLYGTSEAGFCILADPELLQKQPESIGKPITGVQVQI
HCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCEEEECCHHHHHCCHHHHCCCCCCEEEEE
QPDGPSSKGQLCIRSAWSAGAKGWIETGDLAYRDGEGNLFLCGRVDDMIVSGGENVYPLE
ECCCCCCCCCEEEHHHHCCCCCCCEECCCEEEECCCCCEEEEECCCHHHCCCCCCEEEEH
LENVLLQHPGIEAAVVLGIPDPEFGQRLKAIIIPKSAENLEQSTVLAWLKPRVARYQMPA
HHHHHHHCCCCCEEEEEECCCCHHHCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCH
VIEFRQALPYTSLGKLNRKAL
HHHHHHHCCCCHHHCCCCCCC
>Mature Secondary Structure
MIFTLLRKLHRTRLLTLVGLYRLLEAIWTTGVNLMALLRIAAKLHPERIAVIDDQEELTY
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCEEEEECCHHHHHH
SKLWKQAEAVAVGLQRDCGIQPRQRVAIATRNHAAAIKAIFAVSRLGADLFLVNPEMSAD
HHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCHHHHHHHHHHHHHCCCEEEEECCCCCHH
QIRALEHQLQFDYYIYDPELTPIVDDLSLGHKAIPTYHPSSNSVDRMSVMPPSATPLRKV
HHHHHHHHHEEEEEEECCCCCCHHHHHCCCCCCCCCCCCCCCCCCEEEECCCCCCCHHHC
KTGKVVVMTGGTTGQPKPASRKPSLFDFLPPFIALLSQIHLDRYRTVYIATPIYHGFGLA
CCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCEEEEEECCHHHHHHHH
ALLIGVTLGAQQYFTRRFDASRACDLIAQDNIEVITLVPLMLRRMLNQNAQALSSLQRIL
HHHHHHHHCHHHHHHHHCCCHHHHHHHHCCCCEEEHHHHHHHHHHHCCCHHHHHHHHHHH
TGGAALRPALAQATLEQFGPVLFNLYGTSEAGFCILADPELLQKQPESIGKPITGVQVQI
HCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCEEEECCHHHHHCCHHHHCCCCCCEEEEE
QPDGPSSKGQLCIRSAWSAGAKGWIETGDLAYRDGEGNLFLCGRVDDMIVSGGENVYPLE
ECCCCCCCCCEEEHHHHCCCCCCCEECCCEEEECCCCCEEEEECCCHHHCCCCCCEEEEH
LENVLLQHPGIEAAVVLGIPDPEFGQRLKAIIIPKSAENLEQSTVLAWLKPRVARYQMPA
HHHHHHHCCCCCEEEEEECCCCHHHCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCH
VIEFRQALPYTSLGKLNRKAL
HHHHHHHCCCCHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA