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The map label for this gene is insB1 [H]

Identifier: 158334413

GI number: 158334413

Start: 1211742

End: 1212263

Strand: Direct

Name: insB1 [H]

Synonym: AM1_1235

Alternate gene names: 158334413

Gene position: 1211742-1212263 (Clockwise)

Preceding gene: 158334406

Following gene: 158334417

Centisome position: 18.63

GC content: 47.51

Gene sequence:

>522_bases
ATGGGGTTACAAGGTATCGAACGAGTCATAGGCGTTGCTCATACCACTGTGATCCGTTGGGTTCAACAAGTTGGAGTTCT
ATTACCCGATGCTTATGACCCTGACGATATCCCCCAGGTAGGAGAATTAGATGAACTCGAAACCTTCGTGGGCCAAAAAC
GCAACAAGGTCTGGATTTGGACTGTGGTGGATCATTTTCACCCCGGCATTCTAGGCTGGGTTGTGGGTGACCATAGTGCT
GAGACCTTTCGACCGTTATGGCAAGCAATCGTCTGTTGGCAATGTTTTTTCTGGGTCAGTGATGGCAATCCCGTCTATCC
AGGCTTTATCCCAGAGGGTGACCAGATTGTTAGCAAGACCTACATGACACGAGTAGAGGGAGAAAACACGCGTTTACGAC
ATTACTTGGCACGGCTCCATCGCAAGACTTTATGTTATTCAAAGTCGGTGGACATGCTCAAGCATTCAATTCGACTATTG
ATCCACTATCTCAAGTTTGGTGAAGTACCTATTCCTGCGTGA

Upstream 100 bases:

>100_bases
TATTTGTGTGGCCTGTGGACGACAGTTTCTTTCAACTTACTCAAAGCGTGGATACAGTGATTGGACGAAACGACTCTGCC
TGAGAATGTATGTCAACGGA

Downstream 100 bases:

>100_bases
GTCATCCCGTCATTGAGCAACGCCCAAAATTCTACCCCTCTAATATCTGTTTTAACAATCTCCGAATTATTTAGAATGGC
ACCATTTAGATCTAAATCTT

Product: IS1 transposase

Products: NA

Alternate protein names: IS1a [H]

Number of amino acids: Translated: 173; Mature: 172

Protein sequence:

>173_residues
MGLQGIERVIGVAHTTVIRWVQQVGVLLPDAYDPDDIPQVGELDELETFVGQKRNKVWIWTVVDHFHPGILGWVVGDHSA
ETFRPLWQAIVCWQCFFWVSDGNPVYPGFIPEGDQIVSKTYMTRVEGENTRLRHYLARLHRKTLCYSKSVDMLKHSIRLL
IHYLKFGEVPIPA

Sequences:

>Translated_173_residues
MGLQGIERVIGVAHTTVIRWVQQVGVLLPDAYDPDDIPQVGELDELETFVGQKRNKVWIWTVVDHFHPGILGWVVGDHSA
ETFRPLWQAIVCWQCFFWVSDGNPVYPGFIPEGDQIVSKTYMTRVEGENTRLRHYLARLHRKTLCYSKSVDMLKHSIRLL
IHYLKFGEVPIPA
>Mature_172_residues
GLQGIERVIGVAHTTVIRWVQQVGVLLPDAYDPDDIPQVGELDELETFVGQKRNKVWIWTVVDHFHPGILGWVVGDHSAE
TFRPLWQAIVCWQCFFWVSDGNPVYPGFIPEGDQIVSKTYMTRVEGENTRLRHYLARLHRKTLCYSKSVDMLKHSIRLLI
HYLKFGEVPIPA

Specific function: Absolutely required for transposition of IS1 [H]

COG id: COG1662

COG function: function code L; Transposase and inactivated derivatives, IS1 family

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transposase 27 family [H]

Homologues:

Organism=Escherichia coli, GI1789853, Length=118, Percent_Identity=31.3559322033898, Blast_Score=74, Evalue=6e-15,
Organism=Escherichia coli, GI1788203, Length=118, Percent_Identity=31.3559322033898, Blast_Score=74, Evalue=6e-15,
Organism=Escherichia coli, GI1786203, Length=118, Percent_Identity=31.3559322033898, Blast_Score=74, Evalue=6e-15,
Organism=Escherichia coli, GI1787223, Length=118, Percent_Identity=31.3559322033898, Blast_Score=73, Evalue=1e-14,
Organism=Escherichia coli, GI1786470, Length=130, Percent_Identity=31.5384615384615, Blast_Score=73, Evalue=1e-14,
Organism=Escherichia coli, GI1786459, Length=130, Percent_Identity=31.5384615384615, Blast_Score=73, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005063 [H]

Pfam domain/function: PF03400 Transposase_27 [H]

EC number: NA

Molecular weight: Translated: 19937; Mature: 19806

Theoretical pI: Translated: 6.95; Mature: 6.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGLQGIERVIGVAHTTVIRWVQQVGVLLPDAYDPDDIPQVGELDELETFVGQKRNKVWIW
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCEEEE
TVVDHFHPGILGWVVGDHSAETFRPLWQAIVCWQCFFWVSDGNPVYPGFIPEGDQIVSKT
EEHHHHCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHEECCCCEECCCCCCCCHHHHHHH
YMTRVEGENTRLRHYLARLHRKTLCYSKSVDMLKHSIRLLIHYLKFGEVPIPA
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
GLQGIERVIGVAHTTVIRWVQQVGVLLPDAYDPDDIPQVGELDELETFVGQKRNKVWIW
CHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCEEEE
TVVDHFHPGILGWVVGDHSAETFRPLWQAIVCWQCFFWVSDGNPVYPGFIPEGDQIVSKT
EEHHHHCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHEECCCCEECCCCCCCCHHHHHHH
YMTRVEGENTRLRHYLARLHRKTLCYSKSVDMLKHSIRLLIHYLKFGEVPIPA
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 375010; 1849492; 1630901; 9278503 [H]