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The map label for this gene is pchA [H]

Identifier: 158334290

GI number: 158334290

Start: 1086646

End: 1087938

Strand: Reverse

Name: pchA [H]

Synonym: AM1_1109

Alternate gene names: 158334290

Gene position: 1087938-1086646 (Counterclockwise)

Preceding gene: 158334295

Following gene: 158334289

Centisome position: 16.73

GC content: 49.65

Gene sequence:

>1293_bases
TTGGAACTCCCCAAGTTAGACCCACTGGCTGCTCTAGATTTACTGCAACAGGCAGACCATCCTTATTTTTACTGGGAAAA
ACAGTCGCAACGGACTGCGATCGCAGCAACGGAACCATTACTCCATTTACAAATCGACGGAACGCAGAGATTTACCCAGG
CTCAACACTTTATCTGTACTTCTTTGGTGGATACCGTTTCTGCGGGTGCCCTCCACCTTCCCTTTGCAGGAGCTCATTTT
TTCTGCAGTTTTACCTTCTTTGATCACACCAATCAAGAACAGGCGTTTTTCCCCGCAGCTTCCGTTTTTCTTCCCCGCTG
GCAAGTCGCCTGTGTTCAGGACCAAGCAGTACTGGTGGCTAACGCAGTTATTGATACAGACACTGTCTTAGAACCCTTGG
CCCGGAACATCTGGGAACGATGGCAGCTATTACAAACGCCTCGCCGCTGTCCTTCACCCCGATCTCAAGCATCCCAATTC
TATCCCCAGTTAACGAATGCAGACGCTTTTACAACAGCAGTCGCCGATACCTTAGGACTTATTAAGGCTAACAAACTGCA
AAAGCTTGTCTTAGCTCATACCTTAGATGTCAAAACACCTCAACCGCTGGATTTATGCCATGCCTTGGGAACTCTCCGGG
AACGCTATCCAGAATGTTATGTCTTTGCCGTTAGTAATGGCCAAGGCCAGAGCTTTATTGGCGCCAGCCCTGAGCGGCTG
ATTGAATTACATGATCACCACCTGGTGACGGATGCATTGGCCGGATCAGCACCTCGAGGGCTTTCCCATGAAGAGGACCA
GGCCTTAGGAAATCAGCTGCTCAGCAGTAAAAAAGATGGCCATGAACATCGCGTTGTCCTAGACTTCATTCGCGATAGCC
TCTGGGAACTGGGGATTACCCCCCAAGTGACTTCTCTGCCCCATCTGCTCCAGCTCCCTAATATTCAACATTTACGCACC
CTGATCACCGCTGAAGTCCCTCAACATCTGCATCTACTGGAAATTTTGGCAGCCTTACATCCAACCCCTGCTGTAGCAGG
AATGTCACGAGAAGTCGCTCAACAGCAAATTCACAAACAAGAAACCTTTGAACGTCATCTCTATGCAGCTCCTTTAGGGT
GGATTGATTATCAGGGGAATGGTGAATTTACAGTCGGCATTCGGTCCGCATTGATAAACGGCTCCCAAGCCCGGCTCTAT
GCTGGAGCAGGGATTGTGGCTGGCTCTGACCCCCAACGAGAACTGGCCGAAATTCAGCTCAAACTCCATACGCTACTCGA
TGCACTGTTATAG

Upstream 100 bases:

>100_bases
TGCCAATTTATTTCAGGACCGCAGGTCACTCCACGAGTTTCTCGCTTCCTGTCGTACTATCGCCCTTGAGAAGGAACATG
CCCAAGTTGTCAGTCTTTCT

Downstream 100 bases:

>100_bases
GAAGATAAGCTTGTATCAGAAGCGGCTGCAGTCCTCTGCTTTCAACCCAACAGCCTCTTCAGATCAAATTAACTGTATAT
GATGCGAGGACGATGACCCT

Product: isochorismate synthase

Products: NA

Alternate protein names: Isochorismate mutase [H]

Number of amino acids: Translated: 430; Mature: 430

Protein sequence:

>430_residues
MELPKLDPLAALDLLQQADHPYFYWEKQSQRTAIAATEPLLHLQIDGTQRFTQAQHFICTSLVDTVSAGALHLPFAGAHF
FCSFTFFDHTNQEQAFFPAASVFLPRWQVACVQDQAVLVANAVIDTDTVLEPLARNIWERWQLLQTPRRCPSPRSQASQF
YPQLTNADAFTTAVADTLGLIKANKLQKLVLAHTLDVKTPQPLDLCHALGTLRERYPECYVFAVSNGQGQSFIGASPERL
IELHDHHLVTDALAGSAPRGLSHEEDQALGNQLLSSKKDGHEHRVVLDFIRDSLWELGITPQVTSLPHLLQLPNIQHLRT
LITAEVPQHLHLLEILAALHPTPAVAGMSREVAQQQIHKQETFERHLYAAPLGWIDYQGNGEFTVGIRSALINGSQARLY
AGAGIVAGSDPQRELAEIQLKLHTLLDALL

Sequences:

>Translated_430_residues
MELPKLDPLAALDLLQQADHPYFYWEKQSQRTAIAATEPLLHLQIDGTQRFTQAQHFICTSLVDTVSAGALHLPFAGAHF
FCSFTFFDHTNQEQAFFPAASVFLPRWQVACVQDQAVLVANAVIDTDTVLEPLARNIWERWQLLQTPRRCPSPRSQASQF
YPQLTNADAFTTAVADTLGLIKANKLQKLVLAHTLDVKTPQPLDLCHALGTLRERYPECYVFAVSNGQGQSFIGASPERL
IELHDHHLVTDALAGSAPRGLSHEEDQALGNQLLSSKKDGHEHRVVLDFIRDSLWELGITPQVTSLPHLLQLPNIQHLRT
LITAEVPQHLHLLEILAALHPTPAVAGMSREVAQQQIHKQETFERHLYAAPLGWIDYQGNGEFTVGIRSALINGSQARLY
AGAGIVAGSDPQRELAEIQLKLHTLLDALL
>Mature_430_residues
MELPKLDPLAALDLLQQADHPYFYWEKQSQRTAIAATEPLLHLQIDGTQRFTQAQHFICTSLVDTVSAGALHLPFAGAHF
FCSFTFFDHTNQEQAFFPAASVFLPRWQVACVQDQAVLVANAVIDTDTVLEPLARNIWERWQLLQTPRRCPSPRSQASQF
YPQLTNADAFTTAVADTLGLIKANKLQKLVLAHTLDVKTPQPLDLCHALGTLRERYPECYVFAVSNGQGQSFIGASPERL
IELHDHHLVTDALAGSAPRGLSHEEDQALGNQLLSSKKDGHEHRVVLDFIRDSLWELGITPQVTSLPHLLQLPNIQHLRT
LITAEVPQHLHLLEILAALHPTPAVAGMSREVAQQQIHKQETFERHLYAAPLGWIDYQGNGEFTVGIRSALINGSQARLY
AGAGIVAGSDPQRELAEIQLKLHTLLDALL

Specific function: Involved in the conversion of chorismate to salicylate (Probable) [H]

COG id: COG1169

COG function: function code HQ; Isochorismate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isochorismate synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786809, Length=260, Percent_Identity=36.1538461538462, Blast_Score=155, Evalue=3e-39,
Organism=Escherichia coli, GI87082077, Length=249, Percent_Identity=37.3493975903614, Blast_Score=137, Evalue=2e-33,
Organism=Escherichia coli, GI1788114, Length=202, Percent_Identity=27.7227722772277, Blast_Score=90, Evalue=4e-19,
Organism=Escherichia coli, GI1787518, Length=260, Percent_Identity=26.1538461538462, Blast_Score=77, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6320935, Length=216, Percent_Identity=30.0925925925926, Blast_Score=85, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005801
- InterPro:   IPR015890
- InterPro:   IPR004561 [H]

Pfam domain/function: PF00425 Chorismate_bind [H]

EC number: =5.4.4.2 [H]

Molecular weight: Translated: 47767; Mature: 47767

Theoretical pI: Translated: 6.27; Mature: 6.27

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELPKLDPLAALDLLQQADHPYFYWEKQSQRTAIAATEPLLHLQIDGTQRFTQAQHFICT
CCCCCCCHHHHHHHHHHCCCCEEEEECCCCCEEEEECCCEEEEEECCHHHHHHHHHHHHH
SLVDTVSAGALHLPFAGAHFFCSFTFFDHTNQEQAFFPAASVFLPRWQVACVQDQAVLVA
HHHHHHCCCCEECCCCCCEEEEEEEEECCCCCCCHHCCHHHHHCCCCEEEEECCCEEEEE
NAVIDTDTVLEPLARNIWERWQLLQTPRRCPSPRSQASQFYPQLTNADAFTTAVADTLGL
HHHHCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCCCCCHHHHHHHHHHHHH
IKANKLQKLVLAHTLDVKTPQPLDLCHALGTLRERYPECYVFAVSNGQGQSFIGASPERL
HHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCHHHH
IELHDHHLVTDALAGSAPRGLSHEEDQALGNQLLSSKKDGHEHRVVLDFIRDSLWELGIT
HHHHCCHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCC
PQVTSLPHLLQLPNIQHLRTLITAEVPQHLHLLEILAALHPTPAVAGMSREVAQQQIHKQ
CCHHCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCHHHHHHHHHHHH
ETFERHLYAAPLGWIDYQGNGEFTVGIRSALINGSQARLYAGAGIVAGSDPQRELAEIQL
HHHHHHHHCCCCCCEEECCCCCEEEEHHHHHHCCCCEEEEECCEEECCCCHHHHHHHHHH
KLHTLLDALL
HHHHHHHHHC
>Mature Secondary Structure
MELPKLDPLAALDLLQQADHPYFYWEKQSQRTAIAATEPLLHLQIDGTQRFTQAQHFICT
CCCCCCCHHHHHHHHHHCCCCEEEEECCCCCEEEEECCCEEEEEECCHHHHHHHHHHHHH
SLVDTVSAGALHLPFAGAHFFCSFTFFDHTNQEQAFFPAASVFLPRWQVACVQDQAVLVA
HHHHHHCCCCEECCCCCCEEEEEEEEECCCCCCCHHCCHHHHHCCCCEEEEECCCEEEEE
NAVIDTDTVLEPLARNIWERWQLLQTPRRCPSPRSQASQFYPQLTNADAFTTAVADTLGL
HHHHCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCCCCCHHHHHHHHHHHHH
IKANKLQKLVLAHTLDVKTPQPLDLCHALGTLRERYPECYVFAVSNGQGQSFIGASPERL
HHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCHHHH
IELHDHHLVTDALAGSAPRGLSHEEDQALGNQLLSSKKDGHEHRVVLDFIRDSLWELGIT
HHHHCCHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCC
PQVTSLPHLLQLPNIQHLRTLITAEVPQHLHLLEILAALHPTPAVAGMSREVAQQQIHKQ
CCHHCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCHHHHHHHHHHHH
ETFERHLYAAPLGWIDYQGNGEFTVGIRSALINGSQARLYAGAGIVAGSDPQRELAEIQL
HHHHHHHHCCCCCCEEECCCCCEEEEHHHHHHCCCCEEEEECCEEECCCCHHHHHHHHHH
KLHTLLDALL
HHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7500944; 10984043 [H]