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The map label for this gene is pykF [H]

Identifier: 158334134

GI number: 158334134

Start: 919030

End: 920490

Strand: Direct

Name: pykF [H]

Synonym: AM1_0950

Alternate gene names: 158334134

Gene position: 919030-920490 (Clockwise)

Preceding gene: 158334132

Following gene: 158334136

Centisome position: 14.13

GC content: 51.54

Gene sequence:

>1461_bases
TTGAGAGACTGCCTTTACTTCCAAAGAATACCGACCGTGATTGCATCCCTTACGCACCGGACAAAAATCGTCGCTACCAT
TGGTCCGGCCAGTCGTTCTCCAGAAATTATTCGCCAAATGGTGAGGGCCGGAATGGATGTGGCCCGTTTGAATTTTTCCC
ACGGCACCTACGAACAGCATGCCCAAATGGTAAAGCTGCTGCGGTCTGTGTCTGCAGAGCTAGATACGCCCATTACTTTG
CTGCAAGATTTGCAAGGCCCCAAGATTCGAGTAGGCCAGATCACCGATGGGGCCATGAACTTAGTTGCAGGGGAGCGATT
GACGTTAGTAGCGGAAGAGCACTTTCATAATCAGCCCCACACCGTTGCCATTGACTATCCCTACTTGGCGAAAGAAGCTC
AATCCGGAACGCCGATTTTGATGGATGATGGTTTGCTGGAATTACAGGTGGAACAGGTTGAAGGGGATCAAATCCACTGT
CAAATTATTCAAGGCGGCATTTTAGGCAGTCGTAAGGGCGTCAATCTCCCTAGCTTAGATTTACAGCTCCCGTCTCTCAC
GGGTAAGGATCGTCAAGATTTAGAGTTTGGTCTATCCCAAGGGGTGGACTGGGTCTCCCTTAGCTTTGTCCGTCGGGCCG
CCGATATTCATCTCCTCAAAGAGGTGCTAGCGCAGCATGGGGCGGCAGATGTACCGGTGATGGCCAAGATTGAGAAACCC
CATGCGATCGCAAATCTGGAAGCCATATTGGATGCCTGTGATGCGGTGATGGTGGCCCGAGGCGATTTGGGCGTTGAAAT
GAGTGCCGAAAAAGTCCCTAAGCTGCAGAAGCAAATCATTCGGGCCTGTAATAAGCGTAAACTTCCGGTGGTGACCGCCA
CACAAATGCTGGACAGCATGATTCGCAACCCTCGACCGACGCGAGCCGAGGCCAGTGACGTTGCCAACGCCATTATTGAC
GGTACTGATGCCGTGATGCTATCGGGGGAATCGGCTGTAGGCCAATATCCTGTTAAAGCTGTGGAGATGCTGGCCCGGAT
CGCCACGGATGTGGAGCCAGATTTAAACTTCGTCAATAATCCCCCGGCCTGTTTTGATGAGACCCATGCCCTCAGTGAGG
CCCTGAGCGCCATCCAGGATGTCCTGACCTTAAAATGTATTGCCGCGTTTACCTCATCGGGCTACACCGCAATGATGGCA
GCAGGCGAACGCCCCCGAACCCCGGTTATTGCCATTACCCCCTGTGAAAAGGTTTACCATCGCCTTAATTTAGTTTGGGG
CGTCAAGCCCATTTTGATTGACCACCAGGTGGAATCCTTTGAAGAGTTAGTATCTCAAGCCCAATCCTACTTACTGGCTA
AACAGATTGTGGAGTCTGGAGACAAAATTTTGATTATCGGTGGCATTCCTACCCAACGGGCGAGGGGGACCAACTTTATC
AAGATTCATCGGATTGAGTAG

Upstream 100 bases:

>100_bases
GAGACAAATATGGCTCAACAACTACCACGTTAAGAGAGGTTCATGAATGGCAGGATTTTCCTAAGCAATTTTTTAGATCA
GTTATCTATTCAAGATATGG

Downstream 100 bases:

>100_bases
GGGAATTTAGCGCTTTTTTTGGCGCAGCCCCCATTTTTGTAGCTCTACTGCCAAAAAGCCCAGGCTGGCAACCCCAAGAC
AGAGTAGCAGCTCAACAACA

Product: pyruvate kinase

Products: NA

Alternate protein names: PK 1 [H]

Number of amino acids: Translated: 486; Mature: 486

Protein sequence:

>486_residues
MRDCLYFQRIPTVIASLTHRTKIVATIGPASRSPEIIRQMVRAGMDVARLNFSHGTYEQHAQMVKLLRSVSAELDTPITL
LQDLQGPKIRVGQITDGAMNLVAGERLTLVAEEHFHNQPHTVAIDYPYLAKEAQSGTPILMDDGLLELQVEQVEGDQIHC
QIIQGGILGSRKGVNLPSLDLQLPSLTGKDRQDLEFGLSQGVDWVSLSFVRRAADIHLLKEVLAQHGAADVPVMAKIEKP
HAIANLEAILDACDAVMVARGDLGVEMSAEKVPKLQKQIIRACNKRKLPVVTATQMLDSMIRNPRPTRAEASDVANAIID
GTDAVMLSGESAVGQYPVKAVEMLARIATDVEPDLNFVNNPPACFDETHALSEALSAIQDVLTLKCIAAFTSSGYTAMMA
AGERPRTPVIAITPCEKVYHRLNLVWGVKPILIDHQVESFEELVSQAQSYLLAKQIVESGDKILIIGGIPTQRARGTNFI
KIHRIE

Sequences:

>Translated_486_residues
MRDCLYFQRIPTVIASLTHRTKIVATIGPASRSPEIIRQMVRAGMDVARLNFSHGTYEQHAQMVKLLRSVSAELDTPITL
LQDLQGPKIRVGQITDGAMNLVAGERLTLVAEEHFHNQPHTVAIDYPYLAKEAQSGTPILMDDGLLELQVEQVEGDQIHC
QIIQGGILGSRKGVNLPSLDLQLPSLTGKDRQDLEFGLSQGVDWVSLSFVRRAADIHLLKEVLAQHGAADVPVMAKIEKP
HAIANLEAILDACDAVMVARGDLGVEMSAEKVPKLQKQIIRACNKRKLPVVTATQMLDSMIRNPRPTRAEASDVANAIID
GTDAVMLSGESAVGQYPVKAVEMLARIATDVEPDLNFVNNPPACFDETHALSEALSAIQDVLTLKCIAAFTSSGYTAMMA
AGERPRTPVIAITPCEKVYHRLNLVWGVKPILIDHQVESFEELVSQAQSYLLAKQIVESGDKILIIGGIPTQRARGTNFI
KIHRIE
>Mature_486_residues
MRDCLYFQRIPTVIASLTHRTKIVATIGPASRSPEIIRQMVRAGMDVARLNFSHGTYEQHAQMVKLLRSVSAELDTPITL
LQDLQGPKIRVGQITDGAMNLVAGERLTLVAEEHFHNQPHTVAIDYPYLAKEAQSGTPILMDDGLLELQVEQVEGDQIHC
QIIQGGILGSRKGVNLPSLDLQLPSLTGKDRQDLEFGLSQGVDWVSLSFVRRAADIHLLKEVLAQHGAADVPVMAKIEKP
HAIANLEAILDACDAVMVARGDLGVEMSAEKVPKLQKQIIRACNKRKLPVVTATQMLDSMIRNPRPTRAEASDVANAIID
GTDAVMLSGESAVGQYPVKAVEMLARIATDVEPDLNFVNNPPACFDETHALSEALSAIQDVLTLKCIAAFTSSGYTAMMA
AGERPRTPVIAITPCEKVYHRLNLVWGVKPILIDHQVESFEELVSQAQSYLLAKQIVESGDKILIIGGIPTQRARGTNFI
KIHRIE

Specific function: Glycolysis; final step. [C]

COG id: COG0469

COG function: function code G; Pyruvate kinase

Gene ontology:
GO:0000287: Pyruvate kinase
GO:0003824: Pyruvate kinase
GO:0004743: Pyruvate kinase
GO:0006096: Pyruvate kinase
GO:0016301: Pyruvate kinase
GO:0016310: Pyruvate kinase
GO:0016740: Pyruvate kinase
GO:0016772: Pyruvate kinase
GO:0030955: Pyruvate kinase

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pyruvate kinase family [H]

Homologues:

Organism=Homo sapiens, GI33286422, Length=492, Percent_Identity=38.6178861788618, Blast_Score=327, Evalue=2e-89,
Organism=Homo sapiens, GI33286420, Length=492, Percent_Identity=38.6178861788618, Blast_Score=327, Evalue=2e-89,
Organism=Homo sapiens, GI33286418, Length=475, Percent_Identity=38.9473684210526, Blast_Score=324, Evalue=1e-88,
Organism=Homo sapiens, GI10835121, Length=434, Percent_Identity=40.5529953917051, Blast_Score=308, Evalue=1e-83,
Organism=Homo sapiens, GI32967597, Length=434, Percent_Identity=40.5529953917051, Blast_Score=307, Evalue=2e-83,
Organism=Homo sapiens, GI310128732, Length=286, Percent_Identity=40.5594405594406, Blast_Score=219, Evalue=4e-57,
Organism=Homo sapiens, GI310128730, Length=286, Percent_Identity=40.5594405594406, Blast_Score=219, Evalue=4e-57,
Organism=Homo sapiens, GI310128736, Length=234, Percent_Identity=38.4615384615385, Blast_Score=175, Evalue=1e-43,
Organism=Homo sapiens, GI310128734, Length=234, Percent_Identity=38.4615384615385, Blast_Score=175, Evalue=1e-43,
Organism=Homo sapiens, GI310128738, Length=215, Percent_Identity=36.7441860465116, Blast_Score=152, Evalue=6e-37,
Organism=Escherichia coli, GI1787965, Length=470, Percent_Identity=39.3617021276596, Blast_Score=343, Evalue=2e-95,
Organism=Escherichia coli, GI1788160, Length=478, Percent_Identity=36.1924686192469, Blast_Score=273, Evalue=2e-74,
Organism=Caenorhabditis elegans, GI17544584, Length=487, Percent_Identity=38.8090349075975, Blast_Score=322, Evalue=2e-88,
Organism=Caenorhabditis elegans, GI71984413, Length=485, Percent_Identity=36.0824742268041, Blast_Score=290, Evalue=2e-78,
Organism=Caenorhabditis elegans, GI17506829, Length=485, Percent_Identity=36.0824742268041, Blast_Score=290, Evalue=2e-78,
Organism=Caenorhabditis elegans, GI71984406, Length=485, Percent_Identity=36.0824742268041, Blast_Score=289, Evalue=3e-78,
Organism=Caenorhabditis elegans, GI17506831, Length=485, Percent_Identity=36.0824742268041, Blast_Score=288, Evalue=3e-78,
Organism=Saccharomyces cerevisiae, GI6319279, Length=477, Percent_Identity=36.2683438155136, Blast_Score=284, Evalue=2e-77,
Organism=Saccharomyces cerevisiae, GI6324923, Length=469, Percent_Identity=35.6076759061834, Blast_Score=273, Evalue=4e-74,
Organism=Drosophila melanogaster, GI24648964, Length=493, Percent_Identity=37.3225152129817, Blast_Score=309, Evalue=3e-84,
Organism=Drosophila melanogaster, GI28571814, Length=493, Percent_Identity=37.3225152129817, Blast_Score=308, Evalue=6e-84,
Organism=Drosophila melanogaster, GI24648966, Length=413, Percent_Identity=38.498789346247, Blast_Score=281, Evalue=5e-76,
Organism=Drosophila melanogaster, GI24581235, Length=478, Percent_Identity=31.5899581589958, Blast_Score=244, Evalue=1e-64,
Organism=Drosophila melanogaster, GI24646914, Length=307, Percent_Identity=37.4592833876222, Blast_Score=174, Evalue=2e-43,

Paralogues:

None

Copy number: 500 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 124 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001697
- InterPro:   IPR015813
- InterPro:   IPR011037
- InterPro:   IPR015794
- InterPro:   IPR018209
- InterPro:   IPR015793
- InterPro:   IPR015795
- InterPro:   IPR015806 [H]

Pfam domain/function: PF00224 PK; PF02887 PK_C [H]

EC number: =2.7.1.40 [H]

Molecular weight: Translated: 53133; Mature: 53133

Theoretical pI: Translated: 6.28; Mature: 6.28

Prosite motif: PS00110 PYRUVATE_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRDCLYFQRIPTVIASLTHRTKIVATIGPASRSPEIIRQMVRAGMDVARLNFSHGTYEQH
CCCHHHHHHHHHHHHHHHHCEEEEEEECCCCCCHHHHHHHHHCCCCEEEEECCCCCHHHH
AQMVKLLRSVSAELDTPITLLQDLQGPKIRVGQITDGAMNLVAGERLTLVAEEHFHNQPH
HHHHHHHHHHHHHCCCCHHHHHHCCCCCEEEEEECCHHHHHHCCCEEEEEEHHHHCCCCC
TVAIDYPYLAKEAQSGTPILMDDGLLELQVEQVEGDQIHCQIIQGGILGSRKGVNLPSLD
EEEEECCHHHHHCCCCCCEEEECCEEEEEEEECCCCEEEEEEEECCCCCCCCCCCCCCCC
LQLPSLTGKDRQDLEFGLSQGVDWVSLSFVRRAADIHLLKEVLAQHGAADVPVMAKIEKP
EECCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCC
HAIANLEAILDACDAVMVARGDLGVEMSAEKVPKLQKQIIRACNKRKLPVVTATQMLDSM
CHHHHHHHHHHHHHHHEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEHHHHHHHHH
IRNPRPTRAEASDVANAIIDGTDAVMLSGESAVGQYPVKAVEMLARIATDVEPDLNFVNN
HCCCCCCCCCHHHHHHHHHCCCCEEEEECCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCC
PPACFDETHALSEALSAIQDVLTLKCIAAFTSSGYTAMMAAGERPRTPVIAITPCEKVYH
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCEEEECCHHHHHH
RLNLVWGVKPILIDHQVESFEELVSQAQSYLLAKQIVESGDKILIIGGIPTQRARGTNFI
HHHHHHCCCCEEECHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCEE
KIHRIE
EEEECC
>Mature Secondary Structure
MRDCLYFQRIPTVIASLTHRTKIVATIGPASRSPEIIRQMVRAGMDVARLNFSHGTYEQH
CCCHHHHHHHHHHHHHHHHCEEEEEEECCCCCCHHHHHHHHHCCCCEEEEECCCCCHHHH
AQMVKLLRSVSAELDTPITLLQDLQGPKIRVGQITDGAMNLVAGERLTLVAEEHFHNQPH
HHHHHHHHHHHHHCCCCHHHHHHCCCCCEEEEEECCHHHHHHCCCEEEEEEHHHHCCCCC
TVAIDYPYLAKEAQSGTPILMDDGLLELQVEQVEGDQIHCQIIQGGILGSRKGVNLPSLD
EEEEECCHHHHHCCCCCCEEEECCEEEEEEEECCCCEEEEEEEECCCCCCCCCCCCCCCC
LQLPSLTGKDRQDLEFGLSQGVDWVSLSFVRRAADIHLLKEVLAQHGAADVPVMAKIEKP
EECCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCC
HAIANLEAILDACDAVMVARGDLGVEMSAEKVPKLQKQIIRACNKRKLPVVTATQMLDSM
CHHHHHHHHHHHHHHHEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEHHHHHHHHH
IRNPRPTRAEASDVANAIIDGTDAVMLSGESAVGQYPVKAVEMLARIATDVEPDLNFVNN
HCCCCCCCCCHHHHHHHHHCCCCEEEEECCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCC
PPACFDETHALSEALSAIQDVLTLKCIAAFTSSGYTAMMAAGERPRTPVIAITPCEKVYH
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCEEEECCHHHHHH
RLNLVWGVKPILIDHQVESFEELVSQAQSYLLAKQIVESGDKILIIGGIPTQRARGTNFI
HHHHHHCCCCEEECHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCEE
KIHRIE
EEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]