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The map label for this gene is gatB

Identifier: 158334124

GI number: 158334124

Start: 907984

End: 909468

Strand: Direct

Name: gatB

Synonym: AM1_0940

Alternate gene names: 158334124

Gene position: 907984-909468 (Clockwise)

Preceding gene: 158334123

Following gene: 158334125

Centisome position: 13.96

GC content: 47.54

Gene sequence:

>1485_bases
ATGGTCACAAAGGCTCCTGCAAAGACTGAATACGAAGCGGTGATTGGTTTAGAGACCCATTGCCAGCTCAGTACAAAAAC
CAAAATCTTTTGTAACTGCTCCACTGCCTTTGGCGCAGATCCAAATCACCAAGTTTGCTCAATCTGCATGGGTATGCCAG
GTGTTTTACCTGTCCTCAACGAAACCGTTTTAGAATATGCCGTCAAGGCAGGTCGCGCCCTTAATTGTGAGATTGCTAAA
TACAGCAAATTCGACCGGAAGCAATACTTTTACCCCGACCTGCCCAAAAACTATCAAGTCTCCCAATTTGACTTACCCAT
TGCCGAGCATGGTTGGTTAGAAGTCGAAATTGTTGATGAACAAGGGGAAGCCACCCGCAAAAAAATCGGCATTACCCGTC
TACATATGGAAGAAGATGCGGGCAAATTGGTCCATGGCGGCAGCGATCGCCTAGCCGGCTCCACCCACTCTCTGGTGGAC
TTTAACCGAACTGGCATCCCTCTCATAGAGATTGTCTCTGAACCCGATATGCGCACGGGGTTAGAAGCCGCCGAATATGC
CCAAGAGATTCGCCGAATTGTTCGATATCTCGGGGTGAGTGACGGCAATATGCAAGAAGGTTCCCTGCGATGCGACGTCA
ATATTTCTGTTCGTCCTCGGGGACAGAAAGAATTTGGCACCAAAGTAGAAATCAAGAATATGAACTCCTTTAGTGCCATT
CATCGAGCCATCGACTATGAAATTGCTCGTCAAATTGAAGCCATTGAAACCGGTGAACCCATTATTCAAGAAACGCGGCT
ATGGGAAGAAGGCGCACAACGAACCATTGGCATGCGCAGCAAAGAAGGCTCCAGTGACTATCGATATTTCCCAGAACCAG
ACCTAACCCCCATTGAAGTTGCCCCCAGTCTGCTCAAGCAATGGCAATCTGAATTACCTGAACTGCCTGCCGCCAAGCGT
CATCGGTATGAGGAAGAGATTGGGTTGTCCCCTTATGACACCCGTATCCTCACGGATGATCACGCCATCACTCAATATTT
CGAGGCAACCTTAAAAGCAGGCGCTTCGGCCAAACAGGCAGCAAACTGGCTAATGGGAGATATCACTGGCTACCTCAACA
ACGAGAGCTTATCCATTACAGACATTGCCTTAACCCCTGAAGCCTTAGCTGAGCTCATTGAGCTTATCGAAGCCAACACC
ATCAGTGGCAAAATTGCCAAAGAGCTTTTGCCTGAGCTGCTGACAAAAGGCGGTTCTCCTAAAAAACTAGTTAAAAAGAA
AGGACTCACTCAGATCTCGGATGCCGCTACCTTAGAATCTTTGATTGATGAAGTATTAGCAGCCCATCCCCAAGAGCTGG
AACAATACCGCAATGGTAAAACTAAACTGCAAGGCTTTTTCATGGGACAAGTCATGAAACGCACTAGTGGACGGGCAGAT
CCTAAAGCCACGAATCAAATGTTGGCTAAAAAGTTGAAAGGTTAG

Upstream 100 bases:

>100_bases
TTAGTTGACCAAGATTGCGGTTGCGATCACACCTGCCATTCGTTCTCCTCCCCAAACCTTCTAAAATAGGGAAGAGTTAT
TGCAATAAGGCAAACGCTAC

Downstream 100 bases:

>100_bases
ACTTGTGCTTACATCGGAAATCATAAAGCTTTTGAGTAGACATGGGTCTTGAAGTAGTTGAACTCGTGATCGCAATTGAA
GATCGTTTTCGAACGAATCT

Product: aspartyl/glutamyl-tRNA amidotransferase subunit B

Products: NA

Alternate protein names: Asp/Glu-ADT subunit B

Number of amino acids: Translated: 494; Mature: 494

Protein sequence:

>494_residues
MVTKAPAKTEYEAVIGLETHCQLSTKTKIFCNCSTAFGADPNHQVCSICMGMPGVLPVLNETVLEYAVKAGRALNCEIAK
YSKFDRKQYFYPDLPKNYQVSQFDLPIAEHGWLEVEIVDEQGEATRKKIGITRLHMEEDAGKLVHGGSDRLAGSTHSLVD
FNRTGIPLIEIVSEPDMRTGLEAAEYAQEIRRIVRYLGVSDGNMQEGSLRCDVNISVRPRGQKEFGTKVEIKNMNSFSAI
HRAIDYEIARQIEAIETGEPIIQETRLWEEGAQRTIGMRSKEGSSDYRYFPEPDLTPIEVAPSLLKQWQSELPELPAAKR
HRYEEEIGLSPYDTRILTDDHAITQYFEATLKAGASAKQAANWLMGDITGYLNNESLSITDIALTPEALAELIELIEANT
ISGKIAKELLPELLTKGGSPKKLVKKKGLTQISDAATLESLIDEVLAAHPQELEQYRNGKTKLQGFFMGQVMKRTSGRAD
PKATNQMLAKKLKG

Sequences:

>Translated_494_residues
MVTKAPAKTEYEAVIGLETHCQLSTKTKIFCNCSTAFGADPNHQVCSICMGMPGVLPVLNETVLEYAVKAGRALNCEIAK
YSKFDRKQYFYPDLPKNYQVSQFDLPIAEHGWLEVEIVDEQGEATRKKIGITRLHMEEDAGKLVHGGSDRLAGSTHSLVD
FNRTGIPLIEIVSEPDMRTGLEAAEYAQEIRRIVRYLGVSDGNMQEGSLRCDVNISVRPRGQKEFGTKVEIKNMNSFSAI
HRAIDYEIARQIEAIETGEPIIQETRLWEEGAQRTIGMRSKEGSSDYRYFPEPDLTPIEVAPSLLKQWQSELPELPAAKR
HRYEEEIGLSPYDTRILTDDHAITQYFEATLKAGASAKQAANWLMGDITGYLNNESLSITDIALTPEALAELIELIEANT
ISGKIAKELLPELLTKGGSPKKLVKKKGLTQISDAATLESLIDEVLAAHPQELEQYRNGKTKLQGFFMGQVMKRTSGRAD
PKATNQMLAKKLKG
>Mature_494_residues
MVTKAPAKTEYEAVIGLETHCQLSTKTKIFCNCSTAFGADPNHQVCSICMGMPGVLPVLNETVLEYAVKAGRALNCEIAK
YSKFDRKQYFYPDLPKNYQVSQFDLPIAEHGWLEVEIVDEQGEATRKKIGITRLHMEEDAGKLVHGGSDRLAGSTHSLVD
FNRTGIPLIEIVSEPDMRTGLEAAEYAQEIRRIVRYLGVSDGNMQEGSLRCDVNISVRPRGQKEFGTKVEIKNMNSFSAI
HRAIDYEIARQIEAIETGEPIIQETRLWEEGAQRTIGMRSKEGSSDYRYFPEPDLTPIEVAPSLLKQWQSELPELPAAKR
HRYEEEIGLSPYDTRILTDDHAITQYFEATLKAGASAKQAANWLMGDITGYLNNESLSITDIALTPEALAELIELIEANT
ISGKIAKELLPELLTKGGSPKKLVKKKGLTQISDAATLESLIDEVLAAHPQELEQYRNGKTKLQGFFMGQVMKRTSGRAD
PKATNQMLAKKLKG

Specific function: Allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp- tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases. The reaction take

COG id: COG0064

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase B subunit (PET112 homolog)

Gene ontology:
GO:0000166: Aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase subunit B
GO:0005524: Aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase subunit B
GO:0006412: Aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase subunit B
GO:0016874: Aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase subunit B
GO:0016884: Aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase subunit B

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gatB/gatE family. GatB subfamily

Homologues:

Organism=Homo sapiens, GI4758894, Length=500, Percent_Identity=38.8, Blast_Score=327, Evalue=2e-89,
Organism=Caenorhabditis elegans, GI115534094, Length=330, Percent_Identity=39.0909090909091, Blast_Score=233, Evalue=2e-61,
Organism=Saccharomyces cerevisiae, GI6319391, Length=521, Percent_Identity=32.8214971209213, Blast_Score=242, Evalue=9e-65,
Organism=Drosophila melanogaster, GI45550808, Length=500, Percent_Identity=34.6, Blast_Score=288, Evalue=5e-78,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GATB_ACAM1 (B0BZQ6)

Other databases:

- EMBL:   CP000828
- RefSeq:   YP_001515296.1
- ProteinModelPortal:   B0BZQ6
- SMR:   B0BZQ6
- GeneID:   5679766
- GenomeReviews:   CP000828_GR
- KEGG:   amr:AM1_0940
- HOGENOM:   HBG395951
- OMA:   LRYLGTC
- ProtClustDB:   PRK05477
- BioCyc:   AMAR329726:AM1_0940-MONOMER
- GO:   GO:0006412
- HAMAP:   MF_00121
- InterPro:   IPR017959
- InterPro:   IPR018027
- InterPro:   IPR003789
- InterPro:   IPR023168
- InterPro:   IPR004413
- InterPro:   IPR006075
- InterPro:   IPR017958
- Gene3D:   G3DSA:1.10.10.410
- PANTHER:   PTHR11659
- SMART:   SM00845
- TIGRFAMs:   TIGR00133

Pfam domain/function: PF02934 GatB_N; PF02637 GatB_Yqey; SSF89095 GatB_Yqey

EC number: 6.3.5.-

Molecular weight: Translated: 54949; Mature: 54949

Theoretical pI: Translated: 5.72; Mature: 5.72

Prosite motif: PS01234 GATB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVTKAPAKTEYEAVIGLETHCQLSTKTKIFCNCSTAFGADPNHQVCSICMGMPGVLPVLN
CCCCCCCCCCCCEEEECCCCEEECCCEEEEEECCCCCCCCCCHHHHHHHHCCCCHHHHHH
ETVLEYAVKAGRALNCEIAKYSKFDRKQYFYPDLPKNYQVSQFDLPIAEHGWLEVEIVDE
HHHHHHHHHCCCEEEEEHHHHHCCCCCCCCCCCCCCCCCCCEECCCCCCCCEEEEEEECC
QGEATRKKIGITRLHMEEDAGKLVHGGSDRLAGSTHSLVDFNRTGIPLIEIVSEPDMRTG
CCHHHHHHHCCEEEEECCCCCCEEECCCCCCCCCCCHHEECCCCCCCCEEEECCCCHHHC
LEAAEYAQEIRRIVRYLGVSDGNMQEGSLRCDVNISVRPRGQKEFGTKVEIKNMNSFSAI
CHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEEEECCCCCHHCCCEEEEECCHHHHHH
HRAIDYEIARQIEAIETGEPIIQETRLWEEGAQRTIGMRSKEGSSDYRYFPEPDLTPIEV
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHH
APSLLKQWQSELPELPAAKRHRYEEEIGLSPYDTRILTDDHAITQYFEATLKAGASAKQA
HHHHHHHHHHHCCCCCCHHHHCCHHHCCCCCCCCEEECCCHHHHHHHHHHHHCCCCHHHH
ANWLMGDITGYLNNESLSITDIALTPEALAELIELIEANTISGKIAKELLPELLTKGGSP
HHHHHHHHHHHCCCCCEEEEEEEECHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCH
KKLVKKKGLTQISDAATLESLIDEVLAAHPQELEQYRNGKTKLQGFFMGQVMKRTSGRAD
HHHHHHCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCC
PKATNQMLAKKLKG
CHHHHHHHHHHHCC
>Mature Secondary Structure
MVTKAPAKTEYEAVIGLETHCQLSTKTKIFCNCSTAFGADPNHQVCSICMGMPGVLPVLN
CCCCCCCCCCCCEEEECCCCEEECCCEEEEEECCCCCCCCCCHHHHHHHHCCCCHHHHHH
ETVLEYAVKAGRALNCEIAKYSKFDRKQYFYPDLPKNYQVSQFDLPIAEHGWLEVEIVDE
HHHHHHHHHCCCEEEEEHHHHHCCCCCCCCCCCCCCCCCCCEECCCCCCCCEEEEEEECC
QGEATRKKIGITRLHMEEDAGKLVHGGSDRLAGSTHSLVDFNRTGIPLIEIVSEPDMRTG
CCHHHHHHHCCEEEEECCCCCCEEECCCCCCCCCCCHHEECCCCCCCCEEEECCCCHHHC
LEAAEYAQEIRRIVRYLGVSDGNMQEGSLRCDVNISVRPRGQKEFGTKVEIKNMNSFSAI
CHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEEEECCCCCHHCCCEEEEECCHHHHHH
HRAIDYEIARQIEAIETGEPIIQETRLWEEGAQRTIGMRSKEGSSDYRYFPEPDLTPIEV
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHH
APSLLKQWQSELPELPAAKRHRYEEEIGLSPYDTRILTDDHAITQYFEATLKAGASAKQA
HHHHHHHHHHHCCCCCCHHHHCCHHHCCCCCCCCEEECCCHHHHHHHHHHHHCCCCHHHH
ANWLMGDITGYLNNESLSITDIALTPEALAELIELIEANTISGKIAKELLPELLTKGGSP
HHHHHHHHHHHCCCCCEEEEEEEECHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCH
KKLVKKKGLTQISDAATLESLIDEVLAAHPQELEQYRNGKTKLQGFFMGQVMKRTSGRAD
HHHHHHCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCC
PKATNQMLAKKLKG
CHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA