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The map label for this gene is nusA [H]

Identifier: 158334115

GI number: 158334115

Start: 898344

End: 899702

Strand: Direct

Name: nusA [H]

Synonym: AM1_0931

Alternate gene names: 158334115

Gene position: 898344-899702 (Clockwise)

Preceding gene: 158334114

Following gene: 158334116

Centisome position: 13.81

GC content: 50.04

Gene sequence:

>1359_bases
ATGTCCATGGTCCAGTTACCGGGTTTAGCGGAAATGATTAATGTCATCAGCCGTGAACGTAATCTGCCTAAACCTGCGGT
GCAAGCTGCCCTCCAAGAAGCTCTGCTGAAAGGCTATGAACGCTATCGACGCACCCTCCTTTTAGATAGTGGGGCTCAAT
TTGACGAAGAGTATTTTTATAATTTCGATGTCGAACTGGACGTAGAAGAAGAAGGCTTTCTGGTTCTAGCCACCAAAATG
ATCGTGGACGAGGTGGAAAGTACGGACCACCAAATTGCGTTAACCGAAGTGCGGGAAGTTGCCCCCGAAGCTCAACTGGG
TGATACGGTTGTCTTGGATGTGACGCCAGAACAGAATGAATTTGGGCGAATGGCAGCCATCCAAACCAAGCAGGTCCTCT
CCCAAAAACTAAGGGATCAACAGCGCAAGCTGATTCAAGAAGAGTTTCAGGACCTCGAAAAAACCGTATTACAAGCCCGT
GTTCTGAGGTTTGAGCGGCGTTCCGTGATCATGGCTGTCACCAGTGCCTTTGGCCAGCCAGAAGTCGAAGCAGAATTGCC
CAAGCGCGAACAACTTCCCAATGACAACTATCGGGCCAATGCCACCTTTAAAGTATTTCTCAAGCGAGTGGCAGATGGTC
CCCATCGGGGTCCTCAACTGGTGGTCTCTAGAGCCGATGCGGGCTTAGTCGTGTATTTATTTGCAAACGAAGTCCCCGAA
ATAGAAGATGAGGTCGTTCGGATTGTCGCCGTCGCCCGTGAAGCTAATCCCCCCTCCCGCCAGGTCGGTCCCCGCACCAA
AATTGCAGTGGATACCCTTGAACGAGATGTCGATCCCGTCGGGGCTTGTATCGGTGCCCGAGGATCCAGAATTCAGGCTG
TAGTCAATGAACTCAGAGGTGAAAAGATTGACGTCATTCGTTGGTCACCCGATCCAGCCACCTATATTGCCAATGCCCTC
AGCCCTGCTAGAGTTGATGCAGTTCGTCTAATTGATACGGAAGAACGCCAAGCCCATGTCCTCGTTGCCGAAGATCAGCT
CAGCTTAGCCATTGGTAAAGAAGGCCAAAATGTCCGATTAGCGGCCCGTCTAACCGGGTGGAAAATCGATATCAAAGATA
TTGCCAACTATGATCAAGAAGCTGAAGATCTGCAATTGAGCGAACTTCAAGCACAGAAAGTCGATGAGGATGAAACAGCC
GAAATAGAACAACCTGAGCTTGAGACACCTGAAGCATCAGACGCTAGCCCTGAATCAAGTGGCGAAGCTTTAACTGAAGA
ATTTACAGCCCATGCAGATGAGGAAGAAGTCCCAGAAGTAGAGGAGGCAGCCGACACTTCTGAAGATGTGGAAGAGTGA

Upstream 100 bases:

>100_bases
ATTCTTCAGGTGCAGCTCCACGACTCTCCTGAATAACTCCCAAGACATCTACCTGTCTCGAATCTCTCCCTCACACCCTA
GTTCAGCAAGGAGTAAGCCT

Downstream 100 bases:

>100_bases
TACGTTAGATCAACTTTTTGCCCAGACTGGCAAACTGAAGTCACAAATCCTGCCAACAGCTTGCCCTCTTCGATGCGCAG
AATTTCTGAAAGATGCCATG

Product: transcription elongation factor NusA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 452; Mature: 451

Protein sequence:

>452_residues
MSMVQLPGLAEMINVISRERNLPKPAVQAALQEALLKGYERYRRTLLLDSGAQFDEEYFYNFDVELDVEEEGFLVLATKM
IVDEVESTDHQIALTEVREVAPEAQLGDTVVLDVTPEQNEFGRMAAIQTKQVLSQKLRDQQRKLIQEEFQDLEKTVLQAR
VLRFERRSVIMAVTSAFGQPEVEAELPKREQLPNDNYRANATFKVFLKRVADGPHRGPQLVVSRADAGLVVYLFANEVPE
IEDEVVRIVAVAREANPPSRQVGPRTKIAVDTLERDVDPVGACIGARGSRIQAVVNELRGEKIDVIRWSPDPATYIANAL
SPARVDAVRLIDTEERQAHVLVAEDQLSLAIGKEGQNVRLAARLTGWKIDIKDIANYDQEAEDLQLSELQAQKVDEDETA
EIEQPELETPEASDASPESSGEALTEEFTAHADEEEVPEVEEAADTSEDVEE

Sequences:

>Translated_452_residues
MSMVQLPGLAEMINVISRERNLPKPAVQAALQEALLKGYERYRRTLLLDSGAQFDEEYFYNFDVELDVEEEGFLVLATKM
IVDEVESTDHQIALTEVREVAPEAQLGDTVVLDVTPEQNEFGRMAAIQTKQVLSQKLRDQQRKLIQEEFQDLEKTVLQAR
VLRFERRSVIMAVTSAFGQPEVEAELPKREQLPNDNYRANATFKVFLKRVADGPHRGPQLVVSRADAGLVVYLFANEVPE
IEDEVVRIVAVAREANPPSRQVGPRTKIAVDTLERDVDPVGACIGARGSRIQAVVNELRGEKIDVIRWSPDPATYIANAL
SPARVDAVRLIDTEERQAHVLVAEDQLSLAIGKEGQNVRLAARLTGWKIDIKDIANYDQEAEDLQLSELQAQKVDEDETA
EIEQPELETPEASDASPESSGEALTEEFTAHADEEEVPEVEEAADTSEDVEE
>Mature_451_residues
SMVQLPGLAEMINVISRERNLPKPAVQAALQEALLKGYERYRRTLLLDSGAQFDEEYFYNFDVELDVEEEGFLVLATKMI
VDEVESTDHQIALTEVREVAPEAQLGDTVVLDVTPEQNEFGRMAAIQTKQVLSQKLRDQQRKLIQEEFQDLEKTVLQARV
LRFERRSVIMAVTSAFGQPEVEAELPKREQLPNDNYRANATFKVFLKRVADGPHRGPQLVVSRADAGLVVYLFANEVPEI
EDEVVRIVAVAREANPPSRQVGPRTKIAVDTLERDVDPVGACIGARGSRIQAVVNELRGEKIDVIRWSPDPATYIANALS
PARVDAVRLIDTEERQAHVLVAEDQLSLAIGKEGQNVRLAARLTGWKIDIKDIANYDQEAEDLQLSELQAQKVDEDETAE
IEQPELETPEASDASPESSGEALTEEFTAHADEEEVPEVEEAADTSEDVEE

Specific function: Participates in both the termination and antitermination of transcription. Interacts with RNA polymerase and binds RNA [H]

COG id: COG0195

COG function: function code K; Transcription elongation factor

Gene ontology:
GO:0003700: Transcription termination factor NusA
GO:0003723: Transcription termination factor NusA
GO:0006351: Transcription termination factor NusA
GO:0031554: Transcription termination factor NusA

Cell location: Cytoplasmic

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Escherichia coli, GI1789560, Length=361, Percent_Identity=31.8559556786704, Blast_Score=178, Evalue=7e-46,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1612 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004087
- InterPro:   IPR015946
- InterPro:   IPR009019
- InterPro:   IPR004088
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR003029
- InterPro:   IPR022967
- InterPro:   IPR013735
- InterPro:   IPR010213 [H]

Pfam domain/function: PF08529 NusA_N; PF00575 S1 [H]

EC number: NA

Molecular weight: Translated: 50478; Mature: 50347

Theoretical pI: Translated: 4.15; Mature: 4.15

Prosite motif: PS50084 KH_TYPE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSMVQLPGLAEMINVISRERNLPKPAVQAALQEALLKGYERYRRTLLLDSGAQFDEEYFY
CCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCHHEE
NFDVELDVEEEGFLVLATKMIVDEVESTDHQIALTEVREVAPEAQLGDTVVLDVTPEQNE
EEEEEEEECCCCEEEEEHHHHHHHHHCCCCEEHHHHHHHHCCCCCCCCEEEEEECCCCCH
FGRMAAIQTKQVLSQKLRDQQRKLIQEEFQDLEKTVLQARVLRFERRSVIMAVTSAFGQP
HCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
EVEAELPKREQLPNDNYRANATFKVFLKRVADGPHRGPQLVVSRADAGLVVYLFANEVPE
CCCCCCCHHHCCCCCCCCCCHHHHHHHHHHHCCCCCCCEEEEEECCCCEEEEEECCCCCC
IEDEVVRIVAVAREANPPSRQVGPRTKIAVDTLERDVDPVGACIGARGSRIQAVVNELRG
HHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHCCCHHHHHHCCCCHHHHHHHHHHCC
EKIDVIRWSPDPATYIANALSPARVDAVRLIDTEERQAHVLVAEDQLSLAIGKEGQNVRL
CEEEEEEECCCHHHHHHHCCCCHHHCEEEEECCCCCCEEEEEECCCEEEEECCCCCCEEE
AARLTGWKIDIKDIANYDQEAEDLQLSELQAQKVDEDETAEIEQPELETPEASDASPESS
EEEECCCEEEHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCC
GEALTEEFTAHADEEEVPEVEEAADTSEDVEE
CHHHHHHHHCCCCHHCCCCHHHHCCCHHHCCC
>Mature Secondary Structure 
SMVQLPGLAEMINVISRERNLPKPAVQAALQEALLKGYERYRRTLLLDSGAQFDEEYFY
CCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCHHEE
NFDVELDVEEEGFLVLATKMIVDEVESTDHQIALTEVREVAPEAQLGDTVVLDVTPEQNE
EEEEEEEECCCCEEEEEHHHHHHHHHCCCCEEHHHHHHHHCCCCCCCCEEEEEECCCCCH
FGRMAAIQTKQVLSQKLRDQQRKLIQEEFQDLEKTVLQARVLRFERRSVIMAVTSAFGQP
HCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
EVEAELPKREQLPNDNYRANATFKVFLKRVADGPHRGPQLVVSRADAGLVVYLFANEVPE
CCCCCCCHHHCCCCCCCCCCHHHHHHHHHHHCCCCCCCEEEEEECCCCEEEEEECCCCCC
IEDEVVRIVAVAREANPPSRQVGPRTKIAVDTLERDVDPVGACIGARGSRIQAVVNELRG
HHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHCCCHHHHHHCCCCHHHHHHHHHHCC
EKIDVIRWSPDPATYIANALSPARVDAVRLIDTEERQAHVLVAEDQLSLAIGKEGQNVRL
CEEEEEEECCCHHHHHHHCCCCHHHCEEEEECCCCCCEEEEEECCCEEEEECCCCCCEEE
AARLTGWKIDIKDIANYDQEAEDLQLSELQAQKVDEDETAEIEQPELETPEASDASPESS
EEEECCCEEEHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCC
GEALTEEFTAHADEEEVPEVEEAADTSEDVEE
CHHHHHHHHCCCCHHCCCCHHHHCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11058132 [H]