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The map label for this gene is leuD [H]

Identifier: 158334089

GI number: 158334089

Start: 873050

End: 873640

Strand: Reverse

Name: leuD [H]

Synonym: AM1_0904

Alternate gene names: 158334089

Gene position: 873640-873050 (Counterclockwise)

Preceding gene: 158334095

Following gene: 158334084

Centisome position: 13.43

GC content: 50.25

Gene sequence:

>591_bases
ATGAGCCAAATTCATTCCCATACTGGCGCTGGTATTCCCTTGACTGGCAACGATATCGATACGGATCGGATTATTCCTGC
CCGATTCTTGCGATGCGTTACCTTTGACAGCCTGGGACAACAGGTATTTGCAGATGATCGCCAGCAAACGGGAGGCCAGC
ATCCGTTTGATTTACCCCAATTCCAAGGGGCAACGGTATTAGTTGTTAATGGTAATTTCGGCTGTGGCTCGTCTAGGGAA
CATGCCCCCCAAGCGATTTCAAAATGGGGGATTCAGGCGATTATTGGCGAGAGTTTTGCCGAAATCTTTTTTGGGAATTG
TGTGGCAATTGGTATTCCCTGCGTTACGGCTGCTGCTGATGTGGTGCAGCAACTGCAATCCCATCTGACTGAGCATCCTG
GGACGTCGGTGCAAATTAATTTAGACCAAATGACTGTAGCCTGGGGGGATGTGTCCCATCCCATCCATATGGGCGAAGGC
CCTCGACAGATGATGCTGTCGGGTACCTGGGATGCCTGTGGTCAATTGTTGGGGCAGGTCAAACAAATTGAAGCAACGAC
AGAACGGCTCCCTTATTTGGCTTGGAGTTGA

Upstream 100 bases:

>100_bases
ATAAGACCCACTCTCCCCAGACTGACCGGTTGATTTCTTTGACGTCATCGGTAGAAATGATGTCAGAATAAAGACCGACC
TCATCTGTATTACCCCAATC

Downstream 100 bases:

>100_bases
GGTTGCGATCGCACCATGGTTTCCAGGTTTTGCCAATCTTTAGCGTGAATGATCCACGGAGACAGTCTCACTTTCTTCAA
GGCGTCTCCTTGCTGAACAT

Product: isopropylmalate isomerase small subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase [H]

Number of amino acids: Translated: 196; Mature: 195

Protein sequence:

>196_residues
MSQIHSHTGAGIPLTGNDIDTDRIIPARFLRCVTFDSLGQQVFADDRQQTGGQHPFDLPQFQGATVLVVNGNFGCGSSRE
HAPQAISKWGIQAIIGESFAEIFFGNCVAIGIPCVTAAADVVQQLQSHLTEHPGTSVQINLDQMTVAWGDVSHPIHMGEG
PRQMMLSGTWDACGQLLGQVKQIEATTERLPYLAWS

Sequences:

>Translated_196_residues
MSQIHSHTGAGIPLTGNDIDTDRIIPARFLRCVTFDSLGQQVFADDRQQTGGQHPFDLPQFQGATVLVVNGNFGCGSSRE
HAPQAISKWGIQAIIGESFAEIFFGNCVAIGIPCVTAAADVVQQLQSHLTEHPGTSVQINLDQMTVAWGDVSHPIHMGEG
PRQMMLSGTWDACGQLLGQVKQIEATTERLPYLAWS
>Mature_195_residues
SQIHSHTGAGIPLTGNDIDTDRIIPARFLRCVTFDSLGQQVFADDRQQTGGQHPFDLPQFQGATVLVVNGNFGCGSSREH
APQAISKWGIQAIIGESFAEIFFGNCVAIGIPCVTAAADVVQQLQSHLTEHPGTSVQINLDQMTVAWGDVSHPIHMGEGP
RQMMLSGTWDACGQLLGQVKQIEATTERLPYLAWS

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate [H]

COG id: COG0066

COG function: function code E; 3-isopropylmalate dehydratase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leuD family. LeuD type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786258, Length=192, Percent_Identity=34.8958333333333, Blast_Score=117, Evalue=7e-28,
Organism=Saccharomyces cerevisiae, GI6321429, Length=112, Percent_Identity=37.5, Blast_Score=76, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004431
- InterPro:   IPR012305
- InterPro:   IPR015937
- InterPro:   IPR015928
- InterPro:   IPR000573 [H]

Pfam domain/function: PF00694 Aconitase_C [H]

EC number: =4.2.1.33 [H]

Molecular weight: Translated: 21167; Mature: 21036

Theoretical pI: Translated: 5.10; Mature: 5.10

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQIHSHTGAGIPLTGNDIDTDRIIPARFLRCVTFDSLGQQVFADDRQQTGGQHPFDLPQ
CCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCCCCCC
FQGATVLVVNGNFGCGSSREHAPQAISKWGIQAIIGESFAEIFFGNCVAIGIPCVTAAAD
CCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHEECCCHHHHHHH
VVQQLQSHLTEHPGTSVQINLDQMTVAWGDVSHPIHMGEGPRQMMLSGTWDACGQLLGQV
HHHHHHHHHHHCCCCEEEEEECCEEEEECCCCCCEECCCCHHHHEECCCHHHHHHHHHHH
KQIEATTERLPYLAWS
HHHHHHHHHCCCCCCC
>Mature Secondary Structure 
SQIHSHTGAGIPLTGNDIDTDRIIPARFLRCVTFDSLGQQVFADDRQQTGGQHPFDLPQ
CCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCCCCCC
FQGATVLVVNGNFGCGSSREHAPQAISKWGIQAIIGESFAEIFFGNCVAIGIPCVTAAAD
CCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHEECCCHHHHHHH
VVQQLQSHLTEHPGTSVQINLDQMTVAWGDVSHPIHMGEGPRQMMLSGTWDACGQLLGQV
HHHHHHHHHHHCCCCEEEEEECCEEEEECCCCCCEECCCCHHHHEECCCHHHHHHHHHHH
KQIEATTERLPYLAWS
HHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]