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The map label for this gene is leuB [H]

Identifier: 158334082

GI number: 158334082

Start: 867627

End: 868715

Strand: Reverse

Name: leuB [H]

Synonym: AM1_0898

Alternate gene names: 158334082

Gene position: 868715-867627 (Counterclockwise)

Preceding gene: 158334084

Following gene: 158334073

Centisome position: 13.36

GC content: 51.79

Gene sequence:

>1089_bases
ATGACTCAGTCCTATCGAATTACGCTGTTGCCAGGAGACGGTATTGGTCCTGAAATTATGGCCGTGGCCGTTGATGTGCT
CAAGGTTGTAGGGCAACAGCAAAATTTGACCTTTGAATTTAATGAGGCATTGATTGGAGGAGCGGCCATAGATGCGGTGG
AGAACCCGCTACCCAAACAAACCTTAGATGCCTGTAAGCAGAGTGATGCCGTGCTTCTGGCTGCAATTGGGGGCTATAAA
TGGGACACAATGCCCCGCCATCTCCGTCCTGAAACTGGACTTTTGGGATTACGGGCAGGACTAGAACTCTTTGCTAACCT
GAGACCCGCGCAAATTTTGCCCCAACTGATTGATGCGTCCAGTCTGAAGCCAGAGGTGGTTGAGGGCGTAGACATTATGG
TGGTGCGGGAGCTAACGGGCGGCGTCTATTTCGGCTCACCCAAAGGGGTGTTTAGCACCGAAACGGGGGAACAACGCGGC
GTCAATACGATGGCCTATACCGTGGCTGAGATTGATCGGATTGGTCGAGTCGCCTTTGAAACGGCCCAAAAACGGGGTGG
AAAGCTCTGTTCGGTGGATAAAGCCAATGTATTAGAAGTGTCGCAACTGTGGCGCGATCGCATCACCGATCTCTCCTCTG
AATATCCTGATGTAGAACTCTCTCATCTGTATGTAGATAACGCAGCTATGCAGCTGGTCCGCTGGCCTAAACAGTTTGAC
ACTATCGTGACCAGTAATCTATTTGGCGATATTCTCTCGGATATTGCCGCGATGCTCACGGGCAGTATTGGCATGTTGCC
CTCGGCCAGTTTAGGGGCAGATGGCCCAGGGGTATTTGAACCTGTTCATGGCTCTGCCCCGGATATTGCGGGTCAGGATA
AGGCAAATCCTTTGGCCCAAGTGCTGAGTGTGGCGATGATGTTGCGCTATGGATTGGATCAGCCAGCAGCGGCGGATAAA
GTTGAATCTGCTGTGCTGACTTTGTTGGCTCAGGGCTATCGGACAGGTGATATTCAATCTCCAGGTACAACCGTTCTGGG
CTGTAAGGCGATAGGGGAAACCCTGATTCAGATACTGAACCAGGGCTGA

Upstream 100 bases:

>100_bases
AACGATGAGGGACTGAGTCATGAAGATTTTCAAGTTCTGTGGTGTTGGGGATTGCTGCGTCATATGATAGATAGCCTGTG
CGCTTTTGATCGAAACCATC

Downstream 100 bases:

>100_bases
CGGGGAATATAAACACTTAGATCAAGAGAGCAGTCGGACTGCGGAGGTAAAACCCTTTAATTTAGGGCTTCAGCACCACC
AACCACCTCAAGCAGTTCTT

Product: 3-isopropylmalate dehydrogenase

Products: NA

Alternate protein names: 3-IPM-DH; Beta-IPM dehydrogenase; IMDH [H]

Number of amino acids: Translated: 362; Mature: 361

Protein sequence:

>362_residues
MTQSYRITLLPGDGIGPEIMAVAVDVLKVVGQQQNLTFEFNEALIGGAAIDAVENPLPKQTLDACKQSDAVLLAAIGGYK
WDTMPRHLRPETGLLGLRAGLELFANLRPAQILPQLIDASSLKPEVVEGVDIMVVRELTGGVYFGSPKGVFSTETGEQRG
VNTMAYTVAEIDRIGRVAFETAQKRGGKLCSVDKANVLEVSQLWRDRITDLSSEYPDVELSHLYVDNAAMQLVRWPKQFD
TIVTSNLFGDILSDIAAMLTGSIGMLPSASLGADGPGVFEPVHGSAPDIAGQDKANPLAQVLSVAMMLRYGLDQPAAADK
VESAVLTLLAQGYRTGDIQSPGTTVLGCKAIGETLIQILNQG

Sequences:

>Translated_362_residues
MTQSYRITLLPGDGIGPEIMAVAVDVLKVVGQQQNLTFEFNEALIGGAAIDAVENPLPKQTLDACKQSDAVLLAAIGGYK
WDTMPRHLRPETGLLGLRAGLELFANLRPAQILPQLIDASSLKPEVVEGVDIMVVRELTGGVYFGSPKGVFSTETGEQRG
VNTMAYTVAEIDRIGRVAFETAQKRGGKLCSVDKANVLEVSQLWRDRITDLSSEYPDVELSHLYVDNAAMQLVRWPKQFD
TIVTSNLFGDILSDIAAMLTGSIGMLPSASLGADGPGVFEPVHGSAPDIAGQDKANPLAQVLSVAMMLRYGLDQPAAADK
VESAVLTLLAQGYRTGDIQSPGTTVLGCKAIGETLIQILNQG
>Mature_361_residues
TQSYRITLLPGDGIGPEIMAVAVDVLKVVGQQQNLTFEFNEALIGGAAIDAVENPLPKQTLDACKQSDAVLLAAIGGYKW
DTMPRHLRPETGLLGLRAGLELFANLRPAQILPQLIDASSLKPEVVEGVDIMVVRELTGGVYFGSPKGVFSTETGEQRGV
NTMAYTVAEIDRIGRVAFETAQKRGGKLCSVDKANVLEVSQLWRDRITDLSSEYPDVELSHLYVDNAAMQLVRWPKQFDT
IVTSNLFGDILSDIAAMLTGSIGMLPSASLGADGPGVFEPVHGSAPDIAGQDKANPLAQVLSVAMMLRYGLDQPAAADKV
ESAVLTLLAQGYRTGDIQSPGTTVLGCKAIGETLIQILNQG

Specific function: Catalyzes the oxidation of 3-carboxy-2-hydroxy-4- methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2- oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate [H]

COG id: COG0473

COG function: function code CE; Isocitrate/isopropylmalate dehydrogenase

Gene ontology:
GO:0000287: 3-isopropylmalate dehydrogenase
GO:0003862: 3-isopropylmalate dehydrogenase
GO:0005737: 3-isopropylmalate dehydrogenase
GO:0008652: 3-isopropylmalate dehydrogenase
GO:0009082: 3-isopropylmalate dehydrogenase
GO:0009098: 3-isopropylmalate dehydrogenase
GO:0016491: 3-isopropylmalate dehydrogenase
GO:0016616: 3-isopropylmalate dehydrogenase
GO:0046872: 3-isopropylmalate dehydrogenase
GO:0051287: 3-isopropylmalate dehydrogenase
GO:0055114: 3-isopropylmalate dehydrogenase

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate and isopropylmalate dehydrogenases family. LeuB type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI5031777, Length=336, Percent_Identity=32.7380952380952, Blast_Score=151, Evalue=1e-36,
Organism=Homo sapiens, GI28178821, Length=316, Percent_Identity=32.2784810126582, Blast_Score=134, Evalue=2e-31,
Organism=Homo sapiens, GI28178816, Length=316, Percent_Identity=32.2784810126582, Blast_Score=134, Evalue=2e-31,
Organism=Homo sapiens, GI4758582, Length=346, Percent_Identity=29.7687861271676, Blast_Score=120, Evalue=2e-27,
Organism=Homo sapiens, GI28178838, Length=327, Percent_Identity=29.9694189602446, Blast_Score=115, Evalue=4e-26,
Organism=Homo sapiens, GI28178819, Length=148, Percent_Identity=37.8378378378378, Blast_Score=95, Evalue=1e-19,
Organism=Escherichia coli, GI87081683, Length=364, Percent_Identity=48.3516483516484, Blast_Score=347, Evalue=7e-97,
Organism=Escherichia coli, GI1788101, Length=367, Percent_Identity=32.9700272479564, Blast_Score=184, Evalue=8e-48,
Organism=Escherichia coli, GI1787381, Length=305, Percent_Identity=25.5737704918033, Blast_Score=79, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI71986051, Length=343, Percent_Identity=34.402332361516, Blast_Score=161, Evalue=6e-40,
Organism=Caenorhabditis elegans, GI25144293, Length=341, Percent_Identity=32.5513196480938, Blast_Score=130, Evalue=1e-30,
Organism=Caenorhabditis elegans, GI17505779, Length=348, Percent_Identity=31.6091954022989, Blast_Score=129, Evalue=3e-30,
Organism=Caenorhabditis elegans, GI17550882, Length=351, Percent_Identity=29.9145299145299, Blast_Score=128, Evalue=6e-30,
Organism=Saccharomyces cerevisiae, GI6319830, Length=370, Percent_Identity=43.7837837837838, Blast_Score=280, Evalue=3e-76,
Organism=Saccharomyces cerevisiae, GI6322097, Length=359, Percent_Identity=33.1476323119777, Blast_Score=148, Evalue=1e-36,
Organism=Saccharomyces cerevisiae, GI6324709, Length=358, Percent_Identity=34.0782122905028, Blast_Score=144, Evalue=2e-35,
Organism=Saccharomyces cerevisiae, GI6324291, Length=352, Percent_Identity=31.25, Blast_Score=128, Evalue=1e-30,
Organism=Drosophila melanogaster, GI24643270, Length=342, Percent_Identity=31.8713450292398, Blast_Score=154, Evalue=1e-37,
Organism=Drosophila melanogaster, GI24643268, Length=342, Percent_Identity=31.8713450292398, Blast_Score=154, Evalue=1e-37,
Organism=Drosophila melanogaster, GI24661184, Length=356, Percent_Identity=33.1460674157303, Blast_Score=135, Evalue=4e-32,
Organism=Drosophila melanogaster, GI161078637, Length=338, Percent_Identity=27.2189349112426, Blast_Score=112, Evalue=4e-25,
Organism=Drosophila melanogaster, GI161078635, Length=338, Percent_Identity=27.2189349112426, Blast_Score=112, Evalue=5e-25,
Organism=Drosophila melanogaster, GI161078633, Length=338, Percent_Identity=27.2189349112426, Blast_Score=112, Evalue=5e-25,
Organism=Drosophila melanogaster, GI24650122, Length=338, Percent_Identity=27.2189349112426, Blast_Score=112, Evalue=5e-25,
Organism=Drosophila melanogaster, GI161078639, Length=334, Percent_Identity=27.2455089820359, Blast_Score=109, Evalue=4e-24,
Organism=Drosophila melanogaster, GI281362242, Length=355, Percent_Identity=27.3239436619718, Blast_Score=96, Evalue=3e-20,
Organism=Drosophila melanogaster, GI24648872, Length=355, Percent_Identity=27.3239436619718, Blast_Score=96, Evalue=3e-20,
Organism=Drosophila melanogaster, GI20130355, Length=218, Percent_Identity=27.5229357798165, Blast_Score=72, Evalue=5e-13,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019818
- InterPro:   IPR001804
- InterPro:   IPR004429 [H]

Pfam domain/function: PF00180 Iso_dh [H]

EC number: =1.1.1.85 [H]

Molecular weight: Translated: 38578; Mature: 38447

Theoretical pI: Translated: 4.45; Mature: 4.45

Prosite motif: PS00470 IDH_IMDH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQSYRITLLPGDGIGPEIMAVAVDVLKVVGQQQNLTFEFNEALIGGAAIDAVENPLPKQ
CCCCEEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCEEEECHHHHCCHHHHHHCCCCCHH
TLDACKQSDAVLLAAIGGYKWDTMPRHLRPETGLLGLRAGLELFANLRPAQILPQLIDAS
HHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHCCC
SLKPEVVEGVDIMVVRELTGGVYFGSPKGVFSTETGEQRGVNTMAYTVAEIDRIGRVAFE
CCCHHHHCCCCEEEEEECCCCEEECCCCCCCCCCCCCHHCCHHHHHHHHHHHHHHHHHHH
TAQKRGGKLCSVDKANVLEVSQLWRDRITDLSSEYPDVELSHLYVDNAAMQLVRWPKQFD
HHHHCCCCEECCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEEHHHHHHHHHHHHHHHH
TIVTSNLFGDILSDIAAMLTGSIGMLPSASLGADGPGVFEPVHGSAPDIAGQDKANPLAQ
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHH
VLSVAMMLRYGLDQPAAADKVESAVLTLLAQGYRTGDIQSPGTTVLGCKAIGETLIQILN
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEHHHHHHHHHHHHC
QG
CC
>Mature Secondary Structure 
TQSYRITLLPGDGIGPEIMAVAVDVLKVVGQQQNLTFEFNEALIGGAAIDAVENPLPKQ
CCCEEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCEEEECHHHHCCHHHHHHCCCCCHH
TLDACKQSDAVLLAAIGGYKWDTMPRHLRPETGLLGLRAGLELFANLRPAQILPQLIDAS
HHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHCCC
SLKPEVVEGVDIMVVRELTGGVYFGSPKGVFSTETGEQRGVNTMAYTVAEIDRIGRVAFE
CCCHHHHCCCCEEEEEECCCCEEECCCCCCCCCCCCCHHCCHHHHHHHHHHHHHHHHHHH
TAQKRGGKLCSVDKANVLEVSQLWRDRITDLSSEYPDVELSHLYVDNAAMQLVRWPKQFD
HHHHCCCCEECCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEEHHHHHHHHHHHHHHHH
TIVTSNLFGDILSDIAAMLTGSIGMLPSASLGADGPGVFEPVHGSAPDIAGQDKANPLAQ
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHH
VLSVAMMLRYGLDQPAAADKVESAVLTLLAQGYRTGDIQSPGTTVLGCKAIGETLIQILN
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEHHHHHHHHHHHHC
QG
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA