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The map label for this gene is lysC [H]

Identifier: 158333956

GI number: 158333956

Start: 747149

End: 748921

Strand: Direct

Name: lysC [H]

Synonym: AM1_0770

Alternate gene names: 158333956

Gene position: 747149-748921 (Clockwise)

Preceding gene: 158333953

Following gene: 158333957

Centisome position: 11.49

GC content: 53.3

Gene sequence:

>1773_bases
ATGGGCCTAATTGTTCAGAAGTTTGGTGGCACGTCCGTTGGTTCGGTAGAGCGGATCAAAGCGGTTGCTCAACGGGTAAA
GCAGACCGTTGCCCAGGGCAACTCGGTGGTGGTGGTGGTATCGGCCATGGGCAAGAGCACTGATACCCTGGTCCAGCTCG
CCCAAGAAGTATCCTCCAATCCCTGTCGGCGGGAGATGGACATGTTGCTATCGACGGGAGAACAGGTCTCGATTTCCCTG
CTGAGTATGGCCCTCCAAGCACTGGGACAGCCCGCTATTTCCTTGACCGGGGCTCAGGTGGGCATTGTCACAGAGCCTGA
ACATACCCGCGCTCGCATCCTCCACATTGCCACGGAACGATTAGAGCGCCATCTAGAAGAAGGAAAAGTGGTTGTAGTCG
CTGGATTTCAGGGCACTTCTAACACTTCCGATTTGGAAGTGACAACATTAGGGCGAGGGGGATCGGATACCTCTGCGGTG
GCATTAGCAGCGGCCCTTCATGCAGAAAAATGTGAGATCTATACGGATGTAGCTGGGGTCCTCACCACGGACCCTCGGCT
GGTGGAGAAGGCCCAACTGATGACAGAGATTACCTGCGAGGAAATGTTGGAGCTTGCAAGTCTAGGAGCCAACGTACTTC
ATCCTCGGGCCGTAGAAATTGCTCGCAATTATGGGGTGCAGATGGCCGTTCGCTCCAGCTGGACCGATGAACCGGGCACC
CAAATCATTTCAACCGGGACCCAGCCTAAGACCTTAACGGACCTGGAGTTGGGTCGGCCTGTGGATGCGGTGCATTTTGA
TCTGAACCAGGCCAAGGTGGCGTTGCTCCATGTGGCGGATCGTCCCGGTGTCGCAGCTGGGTTGTTTGGCGAGTTAGCCA
ATCAGGGTCTGGATGTGGACCTGATCATTCAATCCATCCATGATGGTAATTCAAATGATATTGCCTTCACCGTTGCCCAG
GACTGTTGTGTTCAAGCGGAGGCTGTGGCAAATGCCTTTAATTCTACGGTGCCTTCTGGAAGTACCTCGGAAGTGCTAGT
CGAACCGAATGTTGCCAAGGTCAGTATTGTCGGGGCAGGGATTATTGGTCGCCCACAAATTGCGGCCCAGATGTTTCAGA
CCCTGAGTGATTTAGGCATTAATTTGCAGATGATTTCCACTTCTGAAGTGAATGTCAGCTGCACCATTACCGCAGCAGAT
TGTGAACGGGCAACCAAGGCTCTGTGCGATGTGTTTGAAGTGACGAGCTCTTCGATTGATAGTGGAATCTCTTTGCCGGA
TTATCCGATTCCTCCAGTGCGAGGGGTGGCCTTGGACTTAGAGCAGGCTCAGTTAGCCATTCGGAATGTCCCGGATCGTC
CGGGCATGGCGGCTCATATTTTCCAGGTCTTGGCCAAGCGAAATATCAGTGTTGATATGATTATTCAGTCCCAGCGCTCC
CGCCAGGTGGAAGGGCAGATTACTCGAGATATCGCCTTTACCGTAGCTGAGGGGGATTTAGAGGTGGCTAAGGGACTCTT
ACAGGATTTGTCCCGCAATCTAGGATGTGGGGAAGTGGCATCCGATTCTGCGATCGCAAAAGTCAGCATTGTTGGTATCG
GTATGTTGGGGCAGCCGGGGGTAGCAGCTCAGATGTTTATGGCCTTAGCCCAGCAGAAAATCAACATTCAAATGATCGCC
ACCTCGGAAATTCGAGTCAGTTGCGTCGTGGCCCAAGGAGATGGGAGTACTGCGTTACAAGCCATTCATCAGGCATTTGG
CCTAGAGGGCTAG

Upstream 100 bases:

>100_bases
ATAACGGTTTCATACCATAATGCTGATTAGCAGTTTGTGGGATATAGAATGGCGTTCTTGCTGGATCTAGTCCTGCGTTT
TACAGATAGATTTAACAATC

Downstream 100 bases:

>100_bases
CTTTTCGGCTGCAAGCTTCACCCTCCCTTATACTGCAAAAAAGAAAATGACCTCGTGATCAAGCGAGGTCATTCAAGGAT
TTGTATTTGGATCTAGGTAA

Product: aspartate kinase

Products: NA

Alternate protein names: Aspartate kinase 2; Aspartokinase II [H]

Number of amino acids: Translated: 590; Mature: 589

Protein sequence:

>590_residues
MGLIVQKFGGTSVGSVERIKAVAQRVKQTVAQGNSVVVVVSAMGKSTDTLVQLAQEVSSNPCRREMDMLLSTGEQVSISL
LSMALQALGQPAISLTGAQVGIVTEPEHTRARILHIATERLERHLEEGKVVVVAGFQGTSNTSDLEVTTLGRGGSDTSAV
ALAAALHAEKCEIYTDVAGVLTTDPRLVEKAQLMTEITCEEMLELASLGANVLHPRAVEIARNYGVQMAVRSSWTDEPGT
QIISTGTQPKTLTDLELGRPVDAVHFDLNQAKVALLHVADRPGVAAGLFGELANQGLDVDLIIQSIHDGNSNDIAFTVAQ
DCCVQAEAVANAFNSTVPSGSTSEVLVEPNVAKVSIVGAGIIGRPQIAAQMFQTLSDLGINLQMISTSEVNVSCTITAAD
CERATKALCDVFEVTSSSIDSGISLPDYPIPPVRGVALDLEQAQLAIRNVPDRPGMAAHIFQVLAKRNISVDMIIQSQRS
RQVEGQITRDIAFTVAEGDLEVAKGLLQDLSRNLGCGEVASDSAIAKVSIVGIGMLGQPGVAAQMFMALAQQKINIQMIA
TSEIRVSCVVAQGDGSTALQAIHQAFGLEG

Sequences:

>Translated_590_residues
MGLIVQKFGGTSVGSVERIKAVAQRVKQTVAQGNSVVVVVSAMGKSTDTLVQLAQEVSSNPCRREMDMLLSTGEQVSISL
LSMALQALGQPAISLTGAQVGIVTEPEHTRARILHIATERLERHLEEGKVVVVAGFQGTSNTSDLEVTTLGRGGSDTSAV
ALAAALHAEKCEIYTDVAGVLTTDPRLVEKAQLMTEITCEEMLELASLGANVLHPRAVEIARNYGVQMAVRSSWTDEPGT
QIISTGTQPKTLTDLELGRPVDAVHFDLNQAKVALLHVADRPGVAAGLFGELANQGLDVDLIIQSIHDGNSNDIAFTVAQ
DCCVQAEAVANAFNSTVPSGSTSEVLVEPNVAKVSIVGAGIIGRPQIAAQMFQTLSDLGINLQMISTSEVNVSCTITAAD
CERATKALCDVFEVTSSSIDSGISLPDYPIPPVRGVALDLEQAQLAIRNVPDRPGMAAHIFQVLAKRNISVDMIIQSQRS
RQVEGQITRDIAFTVAEGDLEVAKGLLQDLSRNLGCGEVASDSAIAKVSIVGIGMLGQPGVAAQMFMALAQQKINIQMIA
TSEIRVSCVVAQGDGSTALQAIHQAFGLEG
>Mature_589_residues
GLIVQKFGGTSVGSVERIKAVAQRVKQTVAQGNSVVVVVSAMGKSTDTLVQLAQEVSSNPCRREMDMLLSTGEQVSISLL
SMALQALGQPAISLTGAQVGIVTEPEHTRARILHIATERLERHLEEGKVVVVAGFQGTSNTSDLEVTTLGRGGSDTSAVA
LAAALHAEKCEIYTDVAGVLTTDPRLVEKAQLMTEITCEEMLELASLGANVLHPRAVEIARNYGVQMAVRSSWTDEPGTQ
IISTGTQPKTLTDLELGRPVDAVHFDLNQAKVALLHVADRPGVAAGLFGELANQGLDVDLIIQSIHDGNSNDIAFTVAQD
CCVQAEAVANAFNSTVPSGSTSEVLVEPNVAKVSIVGAGIIGRPQIAAQMFQTLSDLGINLQMISTSEVNVSCTITAADC
ERATKALCDVFEVTSSSIDSGISLPDYPIPPVRGVALDLEQAQLAIRNVPDRPGMAAHIFQVLAKRNISVDMIIQSQRSR
QVEGQITRDIAFTVAEGDLEVAKGLLQDLSRNLGCGEVASDSAIAKVSIVGIGMLGQPGVAAQMFMALAQQKINIQMIAT
SEIRVSCVVAQGDGSTALQAIHQAFGLEG

Specific function: Catalyzes 2 nonconsecutive reactions in the common biosynthetic pathway leading from Asp to diaminopimelate and Lys, to Met, and to Thr and Ile. [C]

COG id: COG0527

COG function: function code E; Aspartokinases

Gene ontology:
GO:0004072: Aspartokinase
GO:0008152: Aspartokinase
GO:0008652: Aspartokinase
GO:0009089: Aspartokinase
GO:0016301: Aspartokinase
GO:0016310: Aspartokinase
GO:0016597: Aspartokinase
GO:0016740: Aspartokinase

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 ACT domains [H]

Homologues:

Organism=Escherichia coli, GI1786183, Length=351, Percent_Identity=30.7692307692308, Blast_Score=119, Evalue=7e-28,
Organism=Escherichia coli, GI1790455, Length=294, Percent_Identity=30.6122448979592, Blast_Score=91, Evalue=2e-19,
Organism=Escherichia coli, GI1790376, Length=332, Percent_Identity=27.4096385542169, Blast_Score=81, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002912
- InterPro:   IPR001048
- InterPro:   IPR005260
- InterPro:   IPR001341
- InterPro:   IPR018042 [H]

Pfam domain/function: PF00696 AA_kinase; PF01842 ACT [H]

EC number: =2.7.2.4 [H]

Molecular weight: Translated: 62058; Mature: 61927

Theoretical pI: Translated: 4.61; Mature: 4.61

Prosite motif: PS00324 ASPARTOKINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGLIVQKFGGTSVGSVERIKAVAQRVKQTVAQGNSVVVVVSAMGKSTDTLVQLAQEVSSN
CCEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHCCC
PCRREMDMLLSTGEQVSISLLSMALQALGQPAISLTGAQVGIVTEPEHTRARILHIATER
CHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCEEECCCEEEEEECCCHHHHHHHHHHHHH
LERHLEEGKVVVVAGFQGTSNTSDLEVTTLGRGGSDTSAVALAAALHAEKCEIYTDVAGV
HHHHHHCCCEEEEEECCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
LTTDPRLVEKAQLMTEITCEEMLELASLGANVLHPRAVEIARNYGVQMAVRSSWTDEPGT
HCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCEEEEEECCCCCCCCC
QIISTGTQPKTLTDLELGRPVDAVHFDLNQAKVALLHVADRPGVAAGLFGELANQGLDVD
EEEECCCCCCEECCCCCCCCCCEEEECCCCCEEEEEEECCCCCCHHHHHHHHHHCCCCCE
LIIQSIHDGNSNDIAFTVAQDCCVQAEAVANAFNSTVPSGSTSEVLVEPNVAKVSIVGAG
EHEEHHCCCCCCCEEEEEHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCEEEEEEECC
IIGRPQIAAQMFQTLSDLGINLQMISTSEVNVSCTITAADCERATKALCDVFEVTSSSID
CCCCHHHHHHHHHHHHHCCCEEEEEECCCEEEEEEEEEHHHHHHHHHHHHHHHHHHHHHC
SGISLPDYPIPPVRGVALDLEQAQLAIRNVPDRPGMAAHIFQVLAKRNISVDMIIQSQRS
CCCCCCCCCCCCCCCEEECHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCEEEEEECCCC
RQVEGQITRDIAFTVAEGDLEVAKGLLQDLSRNLGCGEVASDSAIAKVSIVGIGMLGQPG
CCCCCEEEEEEEEEEECCCHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEEEEECCCCCC
VAAQMFMALAQQKINIQMIATSEIRVSCVVAQGDGSTALQAIHQAFGLEG
HHHHHHHHHHHCCCEEEEEEECCEEEEEEEEECCCHHHHHHHHHHHCCCC
>Mature Secondary Structure 
GLIVQKFGGTSVGSVERIKAVAQRVKQTVAQGNSVVVVVSAMGKSTDTLVQLAQEVSSN
CEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHCCC
PCRREMDMLLSTGEQVSISLLSMALQALGQPAISLTGAQVGIVTEPEHTRARILHIATER
CHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCEEECCCEEEEEECCCHHHHHHHHHHHHH
LERHLEEGKVVVVAGFQGTSNTSDLEVTTLGRGGSDTSAVALAAALHAEKCEIYTDVAGV
HHHHHHCCCEEEEEECCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
LTTDPRLVEKAQLMTEITCEEMLELASLGANVLHPRAVEIARNYGVQMAVRSSWTDEPGT
HCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCEEEEEECCCCCCCCC
QIISTGTQPKTLTDLELGRPVDAVHFDLNQAKVALLHVADRPGVAAGLFGELANQGLDVD
EEEECCCCCCEECCCCCCCCCCEEEECCCCCEEEEEEECCCCCCHHHHHHHHHHCCCCCE
LIIQSIHDGNSNDIAFTVAQDCCVQAEAVANAFNSTVPSGSTSEVLVEPNVAKVSIVGAG
EHEEHHCCCCCCCEEEEEHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCEEEEEEECC
IIGRPQIAAQMFQTLSDLGINLQMISTSEVNVSCTITAADCERATKALCDVFEVTSSSID
CCCCHHHHHHHHHHHHHCCCEEEEEECCCEEEEEEEEEHHHHHHHHHHHHHHHHHHHHHC
SGISLPDYPIPPVRGVALDLEQAQLAIRNVPDRPGMAAHIFQVLAKRNISVDMIIQSQRS
CCCCCCCCCCCCCCCEEECHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCEEEEEECCCC
RQVEGQITRDIAFTVAEGDLEVAKGLLQDLSRNLGCGEVASDSAIAKVSIVGIGMLGQPG
CCCCCEEEEEEEEEEECCCHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEEEEECCCCCC
VAAQMFMALAQQKINIQMIATSEIRVSCVVAQGDGSTALQAIHQAFGLEG
HHHHHHHHHHHCCCEEEEEEECCEEEEEEEEECCCHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1444390 [H]