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The map label for this gene is fadD25 [H]

Identifier: 158333912

GI number: 158333912

Start: 708174

End: 710054

Strand: Direct

Name: fadD25 [H]

Synonym: AM1_0724

Alternate gene names: 158333912

Gene position: 708174-710054 (Clockwise)

Preceding gene: 158333911

Following gene: 158333913

Centisome position: 10.89

GC content: 52.63

Gene sequence:

>1881_bases
ATGGAGTATAACGTGGCTGAAACAACCAATTGGGACGCCAAGGTCGAAGGAACCTGTCCGCCAAAGGTAGACAGTTTGGT
GGATCTGCTCCGCTATCGAGCGACTCACCAGCCAGACCATAAAGGCTTTACCTTTCTCGTCGATGGTGAAGACGAAACCC
TCCATCTCACCTATGCAGAGCTGGATGCCAAAGCCCGTGCGATCGCAGCTGCGTTACAGTCGGCCTGTCAACCGGGTGAT
CGAGCCCTATTGGTTTATCAGCCTGGATTAGAATATGTGGCCGCTTTCTTTGGCTGCCTCTATGCGGGCGTCACGGCAGT
CCCCATTTATCCACCCCGTCCGAATCGCTCCCTGATGCGATTGCAGAGTGTGATCGCGGACTCCCAAGCCATTTTTGCCC
TGACCACCTCTCAAATTCTGTCGAAGCTAGAACGACAGTTGATTGATGCCCCAGAGTTAAGGGCCCTAAAGTGGTTGGCC
ACTGATAGTCTTAGCGTCACCGAACCTCGTCAAGCTTGGCAAGAGATTCATCCCCAAGGTCAAGATTTAGCCTTTCTTCA
ATACACCTCTGGCTCAACCGCAACGCCCAAAGGGGTGATGATTACCCATCACAATCTGTTGCAGAACCTGGCGCTGATTC
ATCAATGCTTTGAGCATAGTTCTCAGTGCCAAGGCGTGATTTGGCTACCGCCCTACCACGATATGGGCTTGATCGGCGGG
ATTTTGCAGCCTTTATACGGCGGGTTCCCCGTGGCCCTGATGTCTCCCTTTATGTTTTTACAGAGTCCCATTCGCTGGCT
GCAAGCCATTTCTCGCTATCGGGCGACCACCAGTGGGGGACCAAATTTTGCCTACGATTTATGTTTGCGCAAGATCAAGC
CAGAACAGATTGAAACCCTAGATCTAAGCTCCTGGCGGGTGGCCTTTAATGGCGCAGAACCCATCAGTGCTCAAACCCTC
GAAGACTTTGCCACCAAGTTTGCATCCTGTGGATTTCGGCCTGAAGCCTTTTTCCCCTGTTATGGCCTTGCCGAAGCCAC
CCTGATCGTTTCTGGGGGACAGAGACAGGCTAAACCTGCGCTTAAAACGGTCCAAGGGGCAGCCTTAGAACAACATCAAG
TCGTCTCTGCTCACTTAGGAGATACAGATGCTCGCACCTTAGTGGGCTGCGGTACTGCTCTCCAAGGCCAAGAAATTGTG
ATCGCCAATCCTGAAACCCTCCAACAATGTTCAGCCGAAGAAGTCGGCGAAATTTGGGTGTCTGGCCAAAGCATTGCCCA
GGGATACTGGCGGCAATCGGAAGTATCTGGGGAAACCTTTCAGGCCCATTTGGCCGATACCGGAGCTGGCCCCTTTTTAC
GCACCGGAGACTATGGCTTTTTGGTGGGTTCCGAGCTGTTTGTGACCGGACGCCTAAAGGACGTGATTATCATCAATGGC
CGCAATCATTATCCCCAGGACATTGAGTTGACCGTCGTCCAAAGCTATCCGGGGATTCGACCCAATTGTGCTGCAGCGTT
TTCGATTACGGAAGCCGGGGAAGAACGGCTAGTCGTAGTGGCGGAATTGGAGCGGAACTATATCAATCGCTATCAAAAAA
ATGGCAAGAAGCCCTCTTCCCAACCGCCTCAGCTCAATAATTTTGTCCCCCCCACCAGCCTGACTGAGGAATCAGAACCC
GCTGCCGTCGAAAAAGATATTCTCACTCAAATCCGTCGAGCCGTTGCCAAAGACCATGACCTGCAGATTCATGCCCTGCT
GCTGCTCAAACCCGGCAGTATTCCCAAGACCTCTAGTGGTAAAATCCAGCGGTTTACCTGTAGAGCCAAGTTTCTAGAGG
GAGACCTAGAGAGCGTGGCCAAGAGTGTGCCCCGCGCCTAA

Upstream 100 bases:

>100_bases
TCTCCCTATATCAAGGATGCCGAAACCAATCAAAAGAAATGGGACCAGCGGTTACAAGGGCTGTTCAAATCGAAAACGAA
TTCATAATCCTGTAGTCAGG

Downstream 100 bases:

>100_bases
AAGAAAATAGCAACTCTTGGAACTTTTGCAGGCAAAAAGTCAGTCTCAACTTACACACTTATCTCAGGAAGGACATCAGC
CATGCTTACCATGAGTCAGT

Product: AMP-dependent synthetase and ligase

Products: AMP; diphosphate +an acyl-CoA [C]

Alternate protein names: Acyl-CoA synthetase [H]

Number of amino acids: Translated: 626; Mature: 626

Protein sequence:

>626_residues
MEYNVAETTNWDAKVEGTCPPKVDSLVDLLRYRATHQPDHKGFTFLVDGEDETLHLTYAELDAKARAIAAALQSACQPGD
RALLVYQPGLEYVAAFFGCLYAGVTAVPIYPPRPNRSLMRLQSVIADSQAIFALTTSQILSKLERQLIDAPELRALKWLA
TDSLSVTEPRQAWQEIHPQGQDLAFLQYTSGSTATPKGVMITHHNLLQNLALIHQCFEHSSQCQGVIWLPPYHDMGLIGG
ILQPLYGGFPVALMSPFMFLQSPIRWLQAISRYRATTSGGPNFAYDLCLRKIKPEQIETLDLSSWRVAFNGAEPISAQTL
EDFATKFASCGFRPEAFFPCYGLAEATLIVSGGQRQAKPALKTVQGAALEQHQVVSAHLGDTDARTLVGCGTALQGQEIV
IANPETLQQCSAEEVGEIWVSGQSIAQGYWRQSEVSGETFQAHLADTGAGPFLRTGDYGFLVGSELFVTGRLKDVIIING
RNHYPQDIELTVVQSYPGIRPNCAAAFSITEAGEERLVVVAELERNYINRYQKNGKKPSSQPPQLNNFVPPTSLTEESEP
AAVEKDILTQIRRAVAKDHDLQIHALLLLKPGSIPKTSSGKIQRFTCRAKFLEGDLESVAKSVPRA

Sequences:

>Translated_626_residues
MEYNVAETTNWDAKVEGTCPPKVDSLVDLLRYRATHQPDHKGFTFLVDGEDETLHLTYAELDAKARAIAAALQSACQPGD
RALLVYQPGLEYVAAFFGCLYAGVTAVPIYPPRPNRSLMRLQSVIADSQAIFALTTSQILSKLERQLIDAPELRALKWLA
TDSLSVTEPRQAWQEIHPQGQDLAFLQYTSGSTATPKGVMITHHNLLQNLALIHQCFEHSSQCQGVIWLPPYHDMGLIGG
ILQPLYGGFPVALMSPFMFLQSPIRWLQAISRYRATTSGGPNFAYDLCLRKIKPEQIETLDLSSWRVAFNGAEPISAQTL
EDFATKFASCGFRPEAFFPCYGLAEATLIVSGGQRQAKPALKTVQGAALEQHQVVSAHLGDTDARTLVGCGTALQGQEIV
IANPETLQQCSAEEVGEIWVSGQSIAQGYWRQSEVSGETFQAHLADTGAGPFLRTGDYGFLVGSELFVTGRLKDVIIING
RNHYPQDIELTVVQSYPGIRPNCAAAFSITEAGEERLVVVAELERNYINRYQKNGKKPSSQPPQLNNFVPPTSLTEESEP
AAVEKDILTQIRRAVAKDHDLQIHALLLLKPGSIPKTSSGKIQRFTCRAKFLEGDLESVAKSVPRA
>Mature_626_residues
MEYNVAETTNWDAKVEGTCPPKVDSLVDLLRYRATHQPDHKGFTFLVDGEDETLHLTYAELDAKARAIAAALQSACQPGD
RALLVYQPGLEYVAAFFGCLYAGVTAVPIYPPRPNRSLMRLQSVIADSQAIFALTTSQILSKLERQLIDAPELRALKWLA
TDSLSVTEPRQAWQEIHPQGQDLAFLQYTSGSTATPKGVMITHHNLLQNLALIHQCFEHSSQCQGVIWLPPYHDMGLIGG
ILQPLYGGFPVALMSPFMFLQSPIRWLQAISRYRATTSGGPNFAYDLCLRKIKPEQIETLDLSSWRVAFNGAEPISAQTL
EDFATKFASCGFRPEAFFPCYGLAEATLIVSGGQRQAKPALKTVQGAALEQHQVVSAHLGDTDARTLVGCGTALQGQEIV
IANPETLQQCSAEEVGEIWVSGQSIAQGYWRQSEVSGETFQAHLADTGAGPFLRTGDYGFLVGSELFVTGRLKDVIIING
RNHYPQDIELTVVQSYPGIRPNCAAAFSITEAGEERLVVVAELERNYINRYQKNGKKPSSQPPQLNNFVPPTSLTEESEP
AAVEKDILTQIRRAVAKDHDLQIHALLLLKPGSIPKTSSGKIQRFTCRAKFLEGDLESVAKSVPRA

Specific function: Esterification, Concomitant With Transport, Of Exogenous Long-Chain Fatty Acids Into Metabolically Active CoA Thioesters For Subsequent Degradation Or Incorporation Into Phospholipids. [C]

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI44888818, Length=531, Percent_Identity=29.5668549905838, Blast_Score=172, Evalue=1e-42,
Organism=Homo sapiens, GI55749758, Length=599, Percent_Identity=25.7095158597663, Blast_Score=159, Evalue=7e-39,
Organism=Homo sapiens, GI225735629, Length=534, Percent_Identity=28.6516853932584, Blast_Score=157, Evalue=4e-38,
Organism=Homo sapiens, GI45827692, Length=534, Percent_Identity=28.6516853932584, Blast_Score=156, Evalue=5e-38,
Organism=Homo sapiens, GI225735625, Length=644, Percent_Identity=24.5341614906832, Blast_Score=145, Evalue=2e-34,
Organism=Homo sapiens, GI45827694, Length=546, Percent_Identity=25.8241758241758, Blast_Score=136, Evalue=7e-32,
Organism=Homo sapiens, GI225735627, Length=519, Percent_Identity=25.4335260115607, Blast_Score=127, Evalue=4e-29,
Organism=Homo sapiens, GI45827696, Length=519, Percent_Identity=25.4335260115607, Blast_Score=127, Evalue=5e-29,
Organism=Homo sapiens, GI45827698, Length=482, Percent_Identity=25.5186721991701, Blast_Score=114, Evalue=2e-25,
Organism=Escherichia coli, GI145693145, Length=620, Percent_Identity=23.5483870967742, Blast_Score=84, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI193202679, Length=623, Percent_Identity=25.0401284109149, Blast_Score=151, Evalue=1e-36,
Organism=Caenorhabditis elegans, GI17559526, Length=546, Percent_Identity=23.2600732600733, Blast_Score=102, Evalue=8e-22,
Organism=Caenorhabditis elegans, GI17558820, Length=517, Percent_Identity=24.9516441005803, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24654656, Length=486, Percent_Identity=27.5720164609054, Blast_Score=153, Evalue=4e-37,
Organism=Drosophila melanogaster, GI18859661, Length=483, Percent_Identity=25.2587991718427, Blast_Score=86, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: 6.2.1.3 [C]

Molecular weight: Translated: 68736; Mature: 68736

Theoretical pI: Translated: 6.13; Mature: 6.13

Prosite motif: PS00430 TONB_DEPENDENT_REC_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEYNVAETTNWDAKVEGTCPPKVDSLVDLLRYRATHQPDHKGFTFLVDGEDETLHLTYAE
CCCCCCCCCCCCCEECCCCCCCHHHHHHHHHHHCCCCCCCCCEEEEECCCCCEEEEEEHH
LDAKARAIAAALQSACQPGDRALLVYQPGLEYVAAFFGCLYAGVTAVPIYPPRPNRSLMR
HHHHHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHHHCCEEEECCCCCCCHHHHH
LQSVIADSQAIFALTTSQILSKLERQLIDAPELRALKWLATDSLSVTEPRQAWQEIHPQG
HHHHHHCCCEEEEEEHHHHHHHHHHHHCCCCHHHHEEEEECCCCCCCCHHHHHHHHCCCC
QDLAFLQYTSGSTATPKGVMITHHNLLQNLALIHQCFEHSSQCQGVIWLPPYHDMGLIGG
CCEEEEEECCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCHHHHHH
ILQPLYGGFPVALMSPFMFLQSPIRWLQAISRYRATTSGGPNFAYDLCLRKIKPEQIETL
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCHHCEE
DLSSWRVAFNGAEPISAQTLEDFATKFASCGFRPEAFFPCYGLAEATLIVSGGQRQAKPA
ECCCEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEECCCHHHHHH
LKTVQGAALEQHQVVSAHLGDTDARTLVGCGTALQGQEIVIANPETLQQCSAEEVGEIWV
HHHHHCCHHHHHHHHHHHCCCCCCHHEEECCCCCCCCEEEEECCHHHHHCCHHHHHHHEE
SGQSIAQGYWRQSEVSGETFQAHLADTGAGPFLRTGDYGFLVGSELFVTGRLKDVIIING
CCHHHHHHHHHHCCCCCCCEEEEECCCCCCCCEEECCCEEEECCEEEEEECCCEEEEECC
RNHYPQDIELTVVQSYPGIRPNCAAAFSITEAGEERLVVVAELERNYINRYQKNGKKPSS
CCCCCCCEEEEEEECCCCCCCCCEEEEEEECCCCCEEEEEEHHHHHHHHHHHHCCCCCCC
QPPQLNNFVPPTSLTEESEPAAVEKDILTQIRRAVAKDHDLQIHALLLLKPGSIPKTSSG
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCCCCC
KIQRFTCRAKFLEGDLESVAKSVPRA
CEEEEEEHHHHHCCHHHHHHHHCCCC
>Mature Secondary Structure
MEYNVAETTNWDAKVEGTCPPKVDSLVDLLRYRATHQPDHKGFTFLVDGEDETLHLTYAE
CCCCCCCCCCCCCEECCCCCCCHHHHHHHHHHHCCCCCCCCCEEEEECCCCCEEEEEEHH
LDAKARAIAAALQSACQPGDRALLVYQPGLEYVAAFFGCLYAGVTAVPIYPPRPNRSLMR
HHHHHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHHHCCEEEECCCCCCCHHHHH
LQSVIADSQAIFALTTSQILSKLERQLIDAPELRALKWLATDSLSVTEPRQAWQEIHPQG
HHHHHHCCCEEEEEEHHHHHHHHHHHHCCCCHHHHEEEEECCCCCCCCHHHHHHHHCCCC
QDLAFLQYTSGSTATPKGVMITHHNLLQNLALIHQCFEHSSQCQGVIWLPPYHDMGLIGG
CCEEEEEECCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCHHHHHH
ILQPLYGGFPVALMSPFMFLQSPIRWLQAISRYRATTSGGPNFAYDLCLRKIKPEQIETL
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCHHCEE
DLSSWRVAFNGAEPISAQTLEDFATKFASCGFRPEAFFPCYGLAEATLIVSGGQRQAKPA
ECCCEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEECCCHHHHHH
LKTVQGAALEQHQVVSAHLGDTDARTLVGCGTALQGQEIVIANPETLQQCSAEEVGEIWV
HHHHHCCHHHHHHHHHHHCCCCCCHHEEECCCCCCCCEEEEECCHHHHHCCHHHHHHHEE
SGQSIAQGYWRQSEVSGETFQAHLADTGAGPFLRTGDYGFLVGSELFVTGRLKDVIIING
CCHHHHHHHHHHCCCCCCCEEEEECCCCCCCCEEECCCEEEECCEEEEEECCCEEEEECC
RNHYPQDIELTVVQSYPGIRPNCAAAFSITEAGEERLVVVAELERNYINRYQKNGKKPSS
CCCCCCCEEEEEEECCCCCCCCCEEEEEEECCCCCEEEEEEHHHHHHHHHHHHCCCCCCC
QPPQLNNFVPPTSLTEESEPAAVEKDILTQIRRAVAKDHDLQIHALLLLKPGSIPKTSSG
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCCCCC
KIQRFTCRAKFLEGDLESVAKSVPRA
CEEEEEEHHHHHCCHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Salts [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.0408 {octanoate}} 0.0125 {octanoate}} 0.0059 {octanoate}} 0.00072 {octanoate}} 0.0013 {undecanoate}} 0.0022 {pentadecanoate}} 0.053 {CoA}} 0.035 {CoA}} 0.034 {CoA}} 0.0034 {tridecanoate}} 0.002 {laurate}} 0.0019 {laurate}} 0.0

Substrates: ATP; a long-chain carboxylic acid; CoA [C]

Specific reaction: ATP + a long-chain carboxylic acid + CoA = AMP + diphosphate +an acyl-CoA [C]

General reaction: Acid-thiol ligation; Phosphorylation [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]