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The map label for this gene is fadD21 [H]

Identifier: 158333908

GI number: 158333908

Start: 703854

End: 705647

Strand: Direct

Name: fadD21 [H]

Synonym: AM1_0720

Alternate gene names: 158333908

Gene position: 703854-705647 (Clockwise)

Preceding gene: 158333904

Following gene: 158333909

Centisome position: 10.82

GC content: 52.01

Gene sequence:

>1794_bases
ATGACCCAACCCTCAACTCTAGTCGACTTACTGCGAGAGCGGTCTCAGCTCCAGTCTGATTTAACGGCCTATACCTTTTT
GGCCGACGGCAAAAACGAATCCGGGACTCTCACCTACGCCACCTTGGATTTGCAAGCTCGGGCAATTGCAGCCGCAATAC
AGGCCCTAACCGCCCGAGGCACAAACGTTCTGGTCGTTTATCCCTACGATGCCGGACTCGAATTTGTATCCGCATTTTTT
GGCTGTCTCTATGCCGGGGTGGTAGCGGTTACTGATAATCCCCCCCGGAAACCTGAAGCCATTGCCAAGCTCCAAATTCG
GGCGAAAGCCTCGGAAGCCACGGTGTTGGTTTCCACTGCAGGATTTTTGGAGCATCTGAAAAACCAGATGAAAAAGAATC
CCGATTTAGCCCCGTATTTGAATCAATTGACCTGGCTGGCAACGGATACTGTACCCACGGATCAAGCCTCTACCTGGACC
CAGCCGGAGTTAGAAACCGATACTCTAGCCTTCCTCCAATACACCTCGGGTTCCACTGGAGATCCGAAAGGGGTGATGGT
TACCCACGGCAATATGCTCCATAACTCCAAGATGATTTATGAGAGCTTTGGCCATTCCGACCAGAGTCAGGGGGTGATTT
GGCTGCCTCTGTTTCACGATATGGGGTTAGTGGGGGGTGTCCTCCAGCCTTTATATGGTGGCTTTCCGGTGACCTTGATG
TCGCCGATTGCCCTGATCCAAAAACCGATTTTGTGGTTAGAAGCCGTATCTAAATATGGGGCAACCACCAGCGGTGGACC
CAACTTTGCCTACGATTTACTCTGCCAGAAGGTGAAGCCGAAGCAGCGAGACCAGCTAGATCTAAGTAAATGGGAAGTGG
CCTTTTCCGGGGCAGAACCGATCAAAGCTGATACCCTCGATAAGTTTGTGGACTTCTTTGGTCCCTGTGGCTTCCGGCGA
GAAGCTTTTTACCCCTGCTATGGTATGGCTGAAACGACCCTCTTTATTTCTGGGGGCCTGAAGTCAGAATCACCCATCGT
TCGCTATCTCGACAGTACGGCCCTGGCTGACAACAAGGTGGTTCCTGTGGAACCAGAATCTGACAAAGCCCAGGCCATTA
TCGGCTGTGGCCGCATGTGGTTAGGGGATAAGGTTGCGATCGCAAATCCCGATACCCTGACAAAATGTGCGGAAAACCAA
GTCGGTGAGATTTGGGTTGCCGGGGATGGCGTCGGCAAAGGGTACTGGAACAGACCCGACGTCACCGCCGAGGCCTTTCA
GGCCCACTTAGCCGATACGGGCGAAGGACCCTTCCTGCGAACCGGAGATTTAGGCTTTGTCCAGGATGGCGAAGTTTATA
TCACGGGCCGGATTAAAGATTTGATGATTATCTGGGGCCGAAACCAATATCCCCAGCTCATTGAACAGACCGTGGAGCAA
TGCCATCCCGCTCTCCGGCCTAATCATGGTGCCGCTTTTTCCATTCCCCTCAATGGTGAAGAGCATTTGGTGATTGTCCA
TGAGGTGGAACGAAACTTTTTCCGACGCCTCCCCGTCGAAGAGGTTATCGGAGCGATTCGTTTAGCCATTGCCGAGGACC
ATATGGCTGAAGTCTATGCCGTATTGCTCCTCAAGCCAGGTGCAATTCCCAAAACCTCTAGTGGCAAAATTCAACGCCGG
GCTTGTCGGACTAAATTTTTAGAAAGCAGCTTTCAACCTGTTGCCGAGTGGAAACAAGAAACCCAAGGCAATATCTCCGA
TTTGATGGCGCCTATGGCTGCGAATCAACCGTAA

Upstream 100 bases:

>100_bases
AACTACAGTAATGTGATGCAGATCCGCCTTCATTGTGATGCAAGTACTAGCGGGTTTAGATGCTCAAATTTAGGATTTTG
CTACTGCAGATTTTGACCCT

Downstream 100 bases:

>100_bases
TTTAAAGTTCCCCGAATATTGTCCTCCTTGGTGCAAGCCAAGGACCGATTGTCTACCCCAAAACTCCAGTGCTATGAATG
TTCTCAGTCAACCGGATGGA

Product: AMP-dependent synthetase and ligase

Products: AMP; diphosphate +an acyl-CoA [C]

Alternate protein names: Acyl-CoA synthetase [H]

Number of amino acids: Translated: 597; Mature: 596

Protein sequence:

>597_residues
MTQPSTLVDLLRERSQLQSDLTAYTFLADGKNESGTLTYATLDLQARAIAAAIQALTARGTNVLVVYPYDAGLEFVSAFF
GCLYAGVVAVTDNPPRKPEAIAKLQIRAKASEATVLVSTAGFLEHLKNQMKKNPDLAPYLNQLTWLATDTVPTDQASTWT
QPELETDTLAFLQYTSGSTGDPKGVMVTHGNMLHNSKMIYESFGHSDQSQGVIWLPLFHDMGLVGGVLQPLYGGFPVTLM
SPIALIQKPILWLEAVSKYGATTSGGPNFAYDLLCQKVKPKQRDQLDLSKWEVAFSGAEPIKADTLDKFVDFFGPCGFRR
EAFYPCYGMAETTLFISGGLKSESPIVRYLDSTALADNKVVPVEPESDKAQAIIGCGRMWLGDKVAIANPDTLTKCAENQ
VGEIWVAGDGVGKGYWNRPDVTAEAFQAHLADTGEGPFLRTGDLGFVQDGEVYITGRIKDLMIIWGRNQYPQLIEQTVEQ
CHPALRPNHGAAFSIPLNGEEHLVIVHEVERNFFRRLPVEEVIGAIRLAIAEDHMAEVYAVLLLKPGAIPKTSSGKIQRR
ACRTKFLESSFQPVAEWKQETQGNISDLMAPMAANQP

Sequences:

>Translated_597_residues
MTQPSTLVDLLRERSQLQSDLTAYTFLADGKNESGTLTYATLDLQARAIAAAIQALTARGTNVLVVYPYDAGLEFVSAFF
GCLYAGVVAVTDNPPRKPEAIAKLQIRAKASEATVLVSTAGFLEHLKNQMKKNPDLAPYLNQLTWLATDTVPTDQASTWT
QPELETDTLAFLQYTSGSTGDPKGVMVTHGNMLHNSKMIYESFGHSDQSQGVIWLPLFHDMGLVGGVLQPLYGGFPVTLM
SPIALIQKPILWLEAVSKYGATTSGGPNFAYDLLCQKVKPKQRDQLDLSKWEVAFSGAEPIKADTLDKFVDFFGPCGFRR
EAFYPCYGMAETTLFISGGLKSESPIVRYLDSTALADNKVVPVEPESDKAQAIIGCGRMWLGDKVAIANPDTLTKCAENQ
VGEIWVAGDGVGKGYWNRPDVTAEAFQAHLADTGEGPFLRTGDLGFVQDGEVYITGRIKDLMIIWGRNQYPQLIEQTVEQ
CHPALRPNHGAAFSIPLNGEEHLVIVHEVERNFFRRLPVEEVIGAIRLAIAEDHMAEVYAVLLLKPGAIPKTSSGKIQRR
ACRTKFLESSFQPVAEWKQETQGNISDLMAPMAANQP
>Mature_596_residues
TQPSTLVDLLRERSQLQSDLTAYTFLADGKNESGTLTYATLDLQARAIAAAIQALTARGTNVLVVYPYDAGLEFVSAFFG
CLYAGVVAVTDNPPRKPEAIAKLQIRAKASEATVLVSTAGFLEHLKNQMKKNPDLAPYLNQLTWLATDTVPTDQASTWTQ
PELETDTLAFLQYTSGSTGDPKGVMVTHGNMLHNSKMIYESFGHSDQSQGVIWLPLFHDMGLVGGVLQPLYGGFPVTLMS
PIALIQKPILWLEAVSKYGATTSGGPNFAYDLLCQKVKPKQRDQLDLSKWEVAFSGAEPIKADTLDKFVDFFGPCGFRRE
AFYPCYGMAETTLFISGGLKSESPIVRYLDSTALADNKVVPVEPESDKAQAIIGCGRMWLGDKVAIANPDTLTKCAENQV
GEIWVAGDGVGKGYWNRPDVTAEAFQAHLADTGEGPFLRTGDLGFVQDGEVYITGRIKDLMIIWGRNQYPQLIEQTVEQC
HPALRPNHGAAFSIPLNGEEHLVIVHEVERNFFRRLPVEEVIGAIRLAIAEDHMAEVYAVLLLKPGAIPKTSSGKIQRRA
CRTKFLESSFQPVAEWKQETQGNISDLMAPMAANQP

Specific function: Esterification, Concomitant With Transport, Of Exogenous Long-Chain Fatty Acids Into Metabolically Active CoA Thioesters For Subsequent Degradation Or Incorporation Into Phospholipids. [C]

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Partially Membrane-Associated [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI44888818, Length=599, Percent_Identity=26.3772954924875, Blast_Score=149, Evalue=1e-35,
Organism=Homo sapiens, GI225735629, Length=598, Percent_Identity=25.4180602006689, Blast_Score=132, Evalue=1e-30,
Organism=Homo sapiens, GI45827692, Length=598, Percent_Identity=25.4180602006689, Blast_Score=132, Evalue=1e-30,
Organism=Homo sapiens, GI55749758, Length=602, Percent_Identity=25.2491694352159, Blast_Score=130, Evalue=2e-30,
Organism=Homo sapiens, GI225735625, Length=615, Percent_Identity=24.8780487804878, Blast_Score=121, Evalue=2e-27,
Organism=Homo sapiens, GI45827694, Length=545, Percent_Identity=25.1376146788991, Blast_Score=106, Evalue=5e-23,
Organism=Homo sapiens, GI45827696, Length=504, Percent_Identity=25.3968253968254, Blast_Score=103, Evalue=5e-22,
Organism=Homo sapiens, GI225735627, Length=504, Percent_Identity=25.3968253968254, Blast_Score=102, Evalue=7e-22,
Organism=Homo sapiens, GI45827698, Length=481, Percent_Identity=25.1559251559252, Blast_Score=94, Evalue=4e-19,
Organism=Escherichia coli, GI1788107, Length=593, Percent_Identity=25.2951096121417, Blast_Score=119, Evalue=5e-28,
Organism=Caenorhabditis elegans, GI193202679, Length=610, Percent_Identity=24.2622950819672, Blast_Score=149, Evalue=4e-36,
Organism=Caenorhabditis elegans, GI17558820, Length=474, Percent_Identity=24.4725738396624, Blast_Score=103, Evalue=3e-22,
Organism=Drosophila melanogaster, GI24654656, Length=480, Percent_Identity=24.1666666666667, Blast_Score=120, Evalue=2e-27,
Organism=Drosophila melanogaster, GI18859661, Length=518, Percent_Identity=26.2548262548263, Blast_Score=99, Evalue=7e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: 6.2.1.3 [C]

Molecular weight: Translated: 65408; Mature: 65277

Theoretical pI: Translated: 5.16; Mature: 5.16

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQPSTLVDLLRERSQLQSDLTAYTFLADGKNESGTLTYATLDLQARAIAAAIQALTARG
CCCHHHHHHHHHHHHHHHHHHEEEEEEECCCCCCCEEEEEEECHHHHHHHHHHHHHHCCC
TNVLVVYPYDAGLEFVSAFFGCLYAGVVAVTDNPPRKPEAIAKLQIRAKASEATVLVSTA
CEEEEEEECCCCHHHHHHHHHHHHHCEEEECCCCCCCCHHHHEEEEEEECCCCEEEEEHH
GFLEHLKNQMKKNPDLAPYLNQLTWLATDTVPTDQASTWTQPELETDTLAFLQYTSGSTG
HHHHHHHHHHHCCCCHHHHHHHHEEEEECCCCCCCCCCCCCCCCCCCCEEEEEECCCCCC
DPKGVMVTHGNMLHNSKMIYESFGHSDQSQGVIWLPLFHDMGLVGGVLQPLYGGFPVTLM
CCCEEEEECCCCCCCCHHHHHHCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHCCCCHHHH
SPIALIQKPILWLEAVSKYGATTSGGPNFAYDLLCQKVKPKQRDQLDLSKWEVAFSGAEP
HHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCCCCCCCHHEEEEECCCCC
IKADTLDKFVDFFGPCGFRREAFYPCYGMAETTLFISGGLKSESPIVRYLDSTALADNKV
CCHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHCCCCCE
VPVEPESDKAQAIIGCGRMWLGDKVAIANPDTLTKCAENQVGEIWVAGDGVGKGYWNRPD
EECCCCCCCCCEEEECCCEECCCEEEECCCHHHHHHHCCCCCEEEEECCCCCCCCCCCCC
VTAEAFQAHLADTGEGPFLRTGDLGFVQDGEVYITGRIKDLMIIWGRNQYPQLIEQTVEQ
CCHHHHHHHHCCCCCCCEEEECCCCEEECCCEEEEEEEEEEEEEECCCCCHHHHHHHHHH
CHPALRPNHGAAFSIPLNGEEHLVIVHEVERNFFRRLPVEEVIGAIRLAIAEDHMAEVYA
HCCCCCCCCCCEEEEECCCCCCEEEEEHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHH
VLLLKPGAIPKTSSGKIQRRACRTKFLESSFQPVAEWKQETQGNISDLMAPMAANQP
HHEECCCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHCCCHHHHHHHHHCCCC
>Mature Secondary Structure 
TQPSTLVDLLRERSQLQSDLTAYTFLADGKNESGTLTYATLDLQARAIAAAIQALTARG
CCHHHHHHHHHHHHHHHHHHEEEEEEECCCCCCCEEEEEEECHHHHHHHHHHHHHHCCC
TNVLVVYPYDAGLEFVSAFFGCLYAGVVAVTDNPPRKPEAIAKLQIRAKASEATVLVSTA
CEEEEEEECCCCHHHHHHHHHHHHHCEEEECCCCCCCCHHHHEEEEEEECCCCEEEEEHH
GFLEHLKNQMKKNPDLAPYLNQLTWLATDTVPTDQASTWTQPELETDTLAFLQYTSGSTG
HHHHHHHHHHHCCCCHHHHHHHHEEEEECCCCCCCCCCCCCCCCCCCCEEEEEECCCCCC
DPKGVMVTHGNMLHNSKMIYESFGHSDQSQGVIWLPLFHDMGLVGGVLQPLYGGFPVTLM
CCCEEEEECCCCCCCCHHHHHHCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHCCCCHHHH
SPIALIQKPILWLEAVSKYGATTSGGPNFAYDLLCQKVKPKQRDQLDLSKWEVAFSGAEP
HHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCCCCCCCHHEEEEECCCCC
IKADTLDKFVDFFGPCGFRREAFYPCYGMAETTLFISGGLKSESPIVRYLDSTALADNKV
CCHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHCCCCCE
VPVEPESDKAQAIIGCGRMWLGDKVAIANPDTLTKCAENQVGEIWVAGDGVGKGYWNRPD
EECCCCCCCCCEEEECCCEECCCEEEECCCHHHHHHHCCCCCEEEEECCCCCCCCCCCCC
VTAEAFQAHLADTGEGPFLRTGDLGFVQDGEVYITGRIKDLMIIWGRNQYPQLIEQTVEQ
CCHHHHHHHHCCCCCCCEEEECCCCEEECCCEEEEEEEEEEEEEECCCCCHHHHHHHHHH
CHPALRPNHGAAFSIPLNGEEHLVIVHEVERNFFRRLPVEEVIGAIRLAIAEDHMAEVYA
HCCCCCCCCCCEEEEECCCCCCEEEEEHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHH
VLLLKPGAIPKTSSGKIQRRACRTKFLESSFQPVAEWKQETQGNISDLMAPMAANQP
HHEECCCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHCCCHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Salts [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.0408 {octanoate}} 0.0125 {octanoate}} 0.0059 {octanoate}} 0.00072 {octanoate}} 0.0013 {undecanoate}} 0.0022 {pentadecanoate}} 0.053 {CoA}} 0.035 {CoA}} 0.034 {CoA}} 0.0034 {tridecanoate}} 0.002 {laurate}} 0.0019 {laurate}} 0.0

Substrates: ATP; a long-chain carboxylic acid; CoA [C]

Specific reaction: ATP + a long-chain carboxylic acid + CoA = AMP + diphosphate +an acyl-CoA [C]

General reaction: Acid-thiol ligation; Phosphorylation [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]