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The map label for this gene is leuA [H]

Identifier: 158333893

GI number: 158333893

Start: 687170

End: 688810

Strand: Direct

Name: leuA [H]

Synonym: AM1_0705

Alternate gene names: 158333893

Gene position: 687170-688810 (Clockwise)

Preceding gene: 158333892

Following gene: 158333894

Centisome position: 10.57

GC content: 48.57

Gene sequence:

>1641_bases
ATGATCAAATTTCAACCCCAACAGGTCGATCGCATTCTCATTTTCGATACCACCCTTAGAGACGGCGAACAGTCTCCAGG
GGCTACTTTGCATATCGATGAAAAATTGACGATTGCGCGGCAGTTGGCACGGTTGGGGGTTGACATTATTGAAGCCGGTT
TCCCTTTTGCCAGCCCTGGTGATTTTGAAGCAGTCCAGCAGGTGGCTGCGGAGGTCGGCACCGAAAATGGCCCGATCATC
TGTGGTCTAGCCAGAGCCACTGAGCAAGATATTAAAACGGCGGCAAAAGCCTTAAAACCTGCATTCCATCACCGTATCCA
TACCTTCATTGCCACTTCCGATATTCACCTGGAATATAAGCTCAAAAAAACGCGGCCTGAAGTGTTGGCCATCGCCCAAG
ATATGGTGGCTCTCGCTAAGTCCTTTACGGATGATGTGGAGTTTTCCCCAGAAGATGCAGTCCGCTCTGATCCAGAGTTT
CTGTATCAGGTATTGGAAGCTGCGATCGCAGCCGGTGCCACTACCGTTAATATCCCCGATACCGTTGGCTATACAACCCC
TCAAGAATTTGGGGCGCTTATTCGCGGTATTAAGGAAAATGTCCCTAATATCGACCAAGCCGTAATTTCCGTTCACGGCC
ATAATGATCTAGGCTTAGCCGTTGCCAACTTCCTAGAGGCGGTTAAAAACGGGGCTCGCCAACTGGAATGCACCATCAAT
GGCATTGGTGAACGGGCAGGGAATGCTGCCCTTGAAGAATTAGTCATGGCTATGCATGTCCGTCGCCAGTACTATAACCC
TTTCCTCGGACGCCCTCCCGAGTCTGAAGCTCCCCTCACCAATATCGATACTAAACAAATTCATAAAACCTCCCGCTTGG
TCTCTAACCTGACGGGAATGCTGATTCAACCCAATAAGGCGATTGTGGGCACGAATGCCTTTGCCCATGAATCAGGGATT
CATCAAGACGGTGTGCTCAAGCACAAACTCACCTATGAAATTATGGATGCCCAATCCATTGGCTTAGGGGATAACCAAAT
TGTTTTGGGTAAGCATTCTGGTCGAAACGCCTTCCGTACTCGCCTGCAGGAATTGGGGATGGAGTTGTCCGAACAGGATC
TGAATAAAGCCTTCATTCGCTTTAAAGAGCTGGCAGATAAGAAAAAAGAAATTTCCGATCGTGATCTAGAAGCCATCGTT
AAAGACGAAACCCAGTCCCAAGTGATTGGCGGCTTCCGTCTAGAGTTGGTGCAGGTTTCCTGTGGGGATCATGCCTGTCC
CACAGCGACCGTGACTATTTGTACGCCTGGCAACCAAGAGCTTACGGATGCAGCGGTGGGGACTGGACCGGTGGATGCGG
TTTATCAAGCTATTAATCGAGTGGTTAAAGTGCCGAATCGATTAATTGAGTTTTCAGTCCAGTCTGTAACGGCTGGAATT
GACGCGATTGGTGAAGTCACGATTCGTCTAGAGCAGAATGGCAAAATTTATGCCGGATATTCTGCCAATACGGACATTAT
TGTGGCATCCGCTCAGGCTTATCTCAATGCGTTGAATCGTTTATACGACTCAACCCAACCGCAAACTAAAACTCAAAATC
AGGAAACTGATCTTTTGGTAGAGCAAACGGTCCATGCTTAG

Upstream 100 bases:

>100_bases
TATCAGCAAATCTTAAGGTCAGTACTCTAGAATAAGAACATCTAAATTTTAGATAGCGTCCTTTTCTTCTTCTACCTAAA
TATGGGTACTGGATAATATC

Downstream 100 bases:

>100_bases
GGCAGTGAGCCATTGCAATTTTGCAATGGTAGCTCAGCAATATCTGCTGAACTGAAAAGCTGTTCTTATTTAAGTTCATG
ACCCCTGATCAACCTACTCC

Product: 2-isopropylmalate synthase

Products: NA

Alternate protein names: Alpha-IPM synthase; Alpha-isopropylmalate synthase [H]

Number of amino acids: Translated: 546; Mature: 546

Protein sequence:

>546_residues
MIKFQPQQVDRILIFDTTLRDGEQSPGATLHIDEKLTIARQLARLGVDIIEAGFPFASPGDFEAVQQVAAEVGTENGPII
CGLARATEQDIKTAAKALKPAFHHRIHTFIATSDIHLEYKLKKTRPEVLAIAQDMVALAKSFTDDVEFSPEDAVRSDPEF
LYQVLEAAIAAGATTVNIPDTVGYTTPQEFGALIRGIKENVPNIDQAVISVHGHNDLGLAVANFLEAVKNGARQLECTIN
GIGERAGNAALEELVMAMHVRRQYYNPFLGRPPESEAPLTNIDTKQIHKTSRLVSNLTGMLIQPNKAIVGTNAFAHESGI
HQDGVLKHKLTYEIMDAQSIGLGDNQIVLGKHSGRNAFRTRLQELGMELSEQDLNKAFIRFKELADKKKEISDRDLEAIV
KDETQSQVIGGFRLELVQVSCGDHACPTATVTICTPGNQELTDAAVGTGPVDAVYQAINRVVKVPNRLIEFSVQSVTAGI
DAIGEVTIRLEQNGKIYAGYSANTDIIVASAQAYLNALNRLYDSTQPQTKTQNQETDLLVEQTVHA

Sequences:

>Translated_546_residues
MIKFQPQQVDRILIFDTTLRDGEQSPGATLHIDEKLTIARQLARLGVDIIEAGFPFASPGDFEAVQQVAAEVGTENGPII
CGLARATEQDIKTAAKALKPAFHHRIHTFIATSDIHLEYKLKKTRPEVLAIAQDMVALAKSFTDDVEFSPEDAVRSDPEF
LYQVLEAAIAAGATTVNIPDTVGYTTPQEFGALIRGIKENVPNIDQAVISVHGHNDLGLAVANFLEAVKNGARQLECTIN
GIGERAGNAALEELVMAMHVRRQYYNPFLGRPPESEAPLTNIDTKQIHKTSRLVSNLTGMLIQPNKAIVGTNAFAHESGI
HQDGVLKHKLTYEIMDAQSIGLGDNQIVLGKHSGRNAFRTRLQELGMELSEQDLNKAFIRFKELADKKKEISDRDLEAIV
KDETQSQVIGGFRLELVQVSCGDHACPTATVTICTPGNQELTDAAVGTGPVDAVYQAINRVVKVPNRLIEFSVQSVTAGI
DAIGEVTIRLEQNGKIYAGYSANTDIIVASAQAYLNALNRLYDSTQPQTKTQNQETDLLVEQTVHA
>Mature_546_residues
MIKFQPQQVDRILIFDTTLRDGEQSPGATLHIDEKLTIARQLARLGVDIIEAGFPFASPGDFEAVQQVAAEVGTENGPII
CGLARATEQDIKTAAKALKPAFHHRIHTFIATSDIHLEYKLKKTRPEVLAIAQDMVALAKSFTDDVEFSPEDAVRSDPEF
LYQVLEAAIAAGATTVNIPDTVGYTTPQEFGALIRGIKENVPNIDQAVISVHGHNDLGLAVANFLEAVKNGARQLECTIN
GIGERAGNAALEELVMAMHVRRQYYNPFLGRPPESEAPLTNIDTKQIHKTSRLVSNLTGMLIQPNKAIVGTNAFAHESGI
HQDGVLKHKLTYEIMDAQSIGLGDNQIVLGKHSGRNAFRTRLQELGMELSEQDLNKAFIRFKELADKKKEISDRDLEAIV
KDETQSQVIGGFRLELVQVSCGDHACPTATVTICTPGNQELTDAAVGTGPVDAVYQAINRVVKVPNRLIEFSVQSVTAGI
DAIGEVTIRLEQNGKIYAGYSANTDIIVASAQAYLNALNRLYDSTQPQTKTQNQETDLLVEQTVHA

Specific function: Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate) [H]

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:
GO:0003824: 2-isopropylmalate synthase
GO:0003852: 2-isopropylmalate synthase
GO:0008152: 2-isopropylmalate synthase
GO:0008652: 2-isopropylmalate synthase
GO:0009082: 2-isopropylmalate synthase
GO:0009098: 2-isopropylmalate synthase
GO:0016740: 2-isopropylmalate synthase
GO:0019752: 2-isopropylmalate synthase
GO:0046912: 2-isopropylmalate synthase

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786261, Length=524, Percent_Identity=48.4732824427481, Blast_Score=465, Evalue=1e-132,
Organism=Saccharomyces cerevisiae, GI6320071, Length=405, Percent_Identity=28.641975308642, Blast_Score=145, Evalue=1e-35,
Organism=Saccharomyces cerevisiae, GI6320019, Length=399, Percent_Identity=29.3233082706767, Blast_Score=144, Evalue=3e-35,
Organism=Saccharomyces cerevisiae, GI6324225, Length=555, Percent_Identity=24.6846846846847, Blast_Score=113, Evalue=1e-25,
Organism=Saccharomyces cerevisiae, GI6324682, Length=348, Percent_Identity=28.735632183908, Blast_Score=112, Evalue=1e-25,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013709
- InterPro:   IPR002034
- InterPro:   IPR013785
- InterPro:   IPR005671
- InterPro:   IPR000891 [H]

Pfam domain/function: PF00682 HMGL-like; PF08502 LeuA_dimer [H]

EC number: =2.3.3.13 [H]

Molecular weight: Translated: 59530; Mature: 59530

Theoretical pI: Translated: 5.17; Mature: 5.17

Prosite motif: PS00815 AIPM_HOMOCIT_SYNTH_1 ; PS00816 AIPM_HOMOCIT_SYNTH_2 ; PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKFQPQQVDRILIFDTTLRDGEQSPGATLHIDEKLTIARQLARLGVDIIEAGFPFASPG
CCCCCCCCCCEEEEEECCCCCCCCCCCCEEEECCHHHHHHHHHHHCHHHHHCCCCCCCCC
DFEAVQQVAAEVGTENGPIICGLARATEQDIKTAAKALKPAFHHRIHTFIATSDIHLEYK
CHHHHHHHHHHHCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHEEECEEEEEEE
LKKTRPEVLAIAQDMVALAKSFTDDVEFSPEDAVRSDPEFLYQVLEAAIAAGATTVNIPD
ECCCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHCCCHHHHHHHHHHHHHCCCEEECCCC
TVGYTTPQEFGALIRGIKENVPNIDQAVISVHGHNDLGLAVANFLEAVKNGARQLECTIN
CCCCCCHHHHHHHHHHHHHCCCCCCCCEEEEECCCCHHHHHHHHHHHHHCCCEEEEEEEE
GIGERAGNAALEELVMAMHVRRQYYNPFLGRPPESEAPLTNIDTKQIHKTSRLVSNLTGM
CHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCE
LIQPNKAIVGTNAFAHESGIHQDGVLKHKLTYEIMDAQSIGLGDNQIVLGKHSGRNAFRT
EECCCCEEEECCCHHHCCCCCCCCCCHHHHEEEEECCCCCCCCCCEEEEECCCCCHHHHH
RLQELGMELSEQDLNKAFIRFKELADKKKEISDRDLEAIVKDETQSQVIGGFRLELVQVS
HHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCHHHHHHCCEEEEEEEEE
CGDHACPTATVTICTPGNQELTDAAVGTGPVDAVYQAINRVVKVPNRLIEFSVQSVTAGI
CCCCCCCCCEEEEECCCCCCHHHHHCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
DAIGEVTIRLEQNGKIYAGYSANTDIIVASAQAYLNALNRLYDSTQPQTKTQNQETDLLV
HCCCEEEEEEECCCCEEEECCCCCCEEEECHHHHHHHHHHHHCCCCCCCCCCCCHHHHHH
EQTVHA
HHHCCC
>Mature Secondary Structure
MIKFQPQQVDRILIFDTTLRDGEQSPGATLHIDEKLTIARQLARLGVDIIEAGFPFASPG
CCCCCCCCCCEEEEEECCCCCCCCCCCCEEEECCHHHHHHHHHHHCHHHHHCCCCCCCCC
DFEAVQQVAAEVGTENGPIICGLARATEQDIKTAAKALKPAFHHRIHTFIATSDIHLEYK
CHHHHHHHHHHHCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHEEECEEEEEEE
LKKTRPEVLAIAQDMVALAKSFTDDVEFSPEDAVRSDPEFLYQVLEAAIAAGATTVNIPD
ECCCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHCCCHHHHHHHHHHHHHCCCEEECCCC
TVGYTTPQEFGALIRGIKENVPNIDQAVISVHGHNDLGLAVANFLEAVKNGARQLECTIN
CCCCCCHHHHHHHHHHHHHCCCCCCCCEEEEECCCCHHHHHHHHHHHHHCCCEEEEEEEE
GIGERAGNAALEELVMAMHVRRQYYNPFLGRPPESEAPLTNIDTKQIHKTSRLVSNLTGM
CHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCE
LIQPNKAIVGTNAFAHESGIHQDGVLKHKLTYEIMDAQSIGLGDNQIVLGKHSGRNAFRT
EECCCCEEEECCCHHHCCCCCCCCCCHHHHEEEEECCCCCCCCCCEEEEECCCCCHHHHH
RLQELGMELSEQDLNKAFIRFKELADKKKEISDRDLEAIVKDETQSQVIGGFRLELVQVS
HHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCHHHHHHCCEEEEEEEEE
CGDHACPTATVTICTPGNQELTDAAVGTGPVDAVYQAINRVVKVPNRLIEFSVQSVTAGI
CCCCCCCCCEEEEECCCCCCHHHHHCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
DAIGEVTIRLEQNGKIYAGYSANTDIIVASAQAYLNALNRLYDSTQPQTKTQNQETDLLV
HCCCEEEEEEECCCCEEEECCCCCCEEEECHHHHHHHHHHHHCCCCCCCCCCCCHHHHHH
EQTVHA
HHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12240834 [H]